Dorsalization of the neural tube by the non-neural ectoderm

The patterning of cell types along the dorsoventral axis of the spinal cord requires a complex set of inductive signals. While the chordamesoderm is a well-known source of ventralizing signals, relatively little is known about the cues that induce dorsal cell types, including neural crest. Here, we demonstrate that juxtaposition of the non-neural and neural ectoderm is sufficient to induce the expression of dorsal markers, Wnt-1, Wnt-3a and Slug, as well as the formation of neural crest cells. In addition, the competence of neural plate to express Wnt-1 and Wnt-3a appears to be stage dependent, occurring only when neural tissue is taken from stage 8–10 embryos but not from stage 4 embryos, regardless of the age of the non-neural ectoderm. In contrast to the induction of Wnt gene expression, neural crest cell formation and Slug expression can be induced when either stage 4 or stage 8–10 neural plates are placed in contact with the non-neural ectoderm. These data suggest that the non-neural ectoderm provides a signal (or signals) that specifies dorsal cell types within the neural tube, and that the response is dependent on the competence of the neural tissue

Discovery of Selected Water Dispensers by Newborn Pigs

Newborn pigs in 86 litters were offered drinking water from four types of dispenser to determine how quickly each design would be discovered. On average piglets discovered water within about 24 h from an exposed water surface (bowl or cup), whereas discovery time was delayed to more than 72 h with nipple or push-lever dispensers. A prototype dispenser with a wide bowl and continuous bubbling action reduced (P \u3c 0.05) average discovery time to about 14 h

Modifying water nipples for newborn pigs

The use of bite nipples by piglets during the first six days after birth was monitored by time-lapse video recording. Simple bite nipples, mounted at a downward angle, were not discovered by most piglets within the six days, even after the nipple had been modified to drip water continuously. However, two modifications resulted in most piglets discovering the nipple within three days. These modifications were (1) mounting the nipple close to the floor with an upward angle and (2) adding a short length of chain to the valve leve

Silica grain catalysis of methanol formation

The specific catalytic effect of a silica grain on the formation of methanol via the sequential addition of H atoms to CO adsorbed on the surface is investigated. A negatively charged defect on a siliceous edingtonite surface is found to reduce the gas phase barriers for the H + COads and H + H2C=O-ads reactions by 770 and 399 K, respectively, when compared to the same reactions in the gas phase. The catalytic effect of negatively charged surface sites could also be applicable to the hydrogenation of other adsorbed unsaturated species. However, the activation energies on the surface defect are still too large (1150 and 2230 K) for CH3OH to form efficiently at 10-20 K in the interstellar medium via a classical mechanism. It is therefore suggested that quantum mechanical tunnelling through the activation barrier is required for these hydrogen addition reactions to proceed at such temperatures. The calculations show that because the adsorption energies of CO and H2C=O on the negatively charged defect are substantial, CH3OH may form efficiently during the warm-up period in star-forming regions

Soft-tissue specimens from pre-European extinct birds of New Zealand

We provide the first complete review of soft tissue remains from New Zealand birds that became extinct prior to European settlement (c. AD 1800). These rare specimens allow insights into the anatomy and appearance of the birds that are not attainable from bones. Our review includes previously unpublished records of ‘lost’ specimens, and descriptions of recently discovered specimens such as the first evidence of soft tissues from the South Island goose (Cnemiornis calcitrans). Overall, the soft tissue remains are dominated by moa (with specimens from each of the six genera), but also include specimens from Finsch's duck (Chenonetta finschi) and the New Zealand owlet-nightjar (Aegotheles novaezealandiae). All desiccated soft tissue specimens that have radiocarbon or stratigraphic dates are late Holocene in age, and most have been found in the semi-arid region of Central Otago

A Memetic Algorithm for whole test suite generation

The generation of unit-level test cases for structural code coverage is a task well-suited to Genetic Algorithms. Method call sequences must be created that construct objects, put them into the right state and then execute uncovered code. However, the generation of primitive values, such as integers and doubles, characters that appear in strings, and arrays of primitive values, are not so straightforward. Often, small local changes are required to drive the value toward the one needed to execute some target structure. However, global searches like Genetic Algorithms tend to make larger changes that are not concentrated on any particular aspect of a test case. In this paper, we extend the Genetic Algorithm behind the EvoSuiTE test generation tool into a Memetic Algorithm, by equipping it with several local search operators. These operators are designed to efficiently optimize primitive values and other aspects of a test suite that allow the search for test cases to function more effectively. We evaluate our operators using a rigorous experimental methodology on over 12,000 Java classes, comprising open source classes of various different kinds, including numerical applications and text processors. Our study shows that increases in branch coverage of up to 53% are possible for an individual class in practice