Regulation of stearoyl-CoA desaturase-1 after central and peripheral nerve lesions

BACKGROUND: Interruption of mature axons activates a cascade of events in neuronal cell bodies which leads to various outcomes from functional regeneration in the PNS to the failure of any significant regeneration in the CNS. One factor which seems to play an important role in the molecular programs after axotomy is the stearoyl Coenzyme A-desaturase-1 (SCD-1). This enzyme is needed for the conversion of stearate into oleate. Beside its role in membrane synthesis, oleate could act as a neurotrophic factor, involved in signal transduction pathways via activation of protein kinases C. RESULTS: In situ hybridization and immunohistochemistry demonstrated a strong up-regulation of SCD at mRNA and protein level in regenerating neurons of the rat facial nucleus whereas non-regenerating Clarke's and Red nucleus neurons did not show an induction of this gene. CONCLUSION: This differential expression points to a functionally significant role for the SCD-1 in the process of regeneration

Longitudinal Pathways between Maternal Mental Health in Infancy and Offspring Romantic Relationships in Adulthood: A 30‐year Prospective Study

Longitudinal pathways between maternal mental health in infancy and offspring romantic relationship outcomes in adulthood were examined using a 30‐year prospective longitudinal study of 196 mothers and their children. Structural equation modeling revealed that maternal mental health at 30 months was related to offspring relationship status and relationship attachment insecurity at 30 years. The adolescent variables of offspring mental health and parental relationship status mediated the link between maternal mental health and offspring relationship status. In addition, adolescent mental health and family conflict mediated the pathway between maternal mental health and offspring attachment insecurity. These results highlight the importance of individual and parental mental health to romantic relationship outcomes, and emphasize the value of taking a developmental approach to the study of romantic relationships.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90142/1/j.1467-9507.2011.00610.x.pd

Implementing a dynamic riparian vegetation model in three European river systems

Riparian ecosystems are required to be preserved to achieve a good ecological status. The Water Framework Directive (2000/60/EC) specifically supports the assessment of new management tools that allow the European Member States to achieve good ecological status of river-related ecosystems. Within several approaches, a dynamic riparian vegetation distributed model (CASiMiR-vegetation), with a time step of 1 year, has been selected as a useful first-step tool to achieve the Water Framework Directive requirements. The model has been implemented into three river reaches with different climatic and hydrologic settings, located in three European countries. Common bases were established in the model setup. The model was calibrated independently in the Kleblach reach (Drau River, Austria), the Ribeira reach (Odelouca River, Portugal), and the Terde reach (Mijares River, Spain) with simulation periods of 8, 11 and 41 years, respectively. The parameter values and the results were comparable between the different countries. The calibration performance achieved high correctly classified instances (60%). Additionally, weighted kappa values ranged from 0·52 to 0·66 in distinguishing riparian succession phases. The model behaved similarly in the validation, even offering better results in most cases. This work demonstrates the applicability of this model in the simulation of the riparian vegetation dynamic distribution over a wide range of environments. As it performs in a robust manner and with good results in reaches with different hydrological characteristics, the model could be also applied to analyse different hydrological scenarios or to predict changes after restoration measures within a reach. Copyright © 2012 John Wiley & Sons, Ltd.The Austrian team would like to thank the Lebensministerium (Austrian Ministry of Environment) and Professor Helmut Habersack and his group from the University of Natural Resources and Life Science Vienna for the supply of the hydrological data. The Portuguese team would like to thank Antonio Pinheiro for his supervision of the hydraulic modelling. The hydrological data were supplied by the Portuguese National Hydrologic Resource Information System (SNIRH) and aerial photographs by the Portuguese Geographic Institute (IGP) under the FIGIEE programme. Patricia M. Rodriguez-Gonzalez benefited from a postdoctoral grant from FCT (SFRH/BPD/47140/2008). Antonio Albuquerque was a valuable assistant in field work. The Spanish team would like to thank in addition the Hydrological Studies Centre (CEH-CEDEX), the Jucar River Basin Authority (CHJ) and the Spanish National Geographic Information Centre (CNIG) for supplying the hydrological data and the aerial photographs for the Spanish study site.García-Arias, A.; Francés García, FR.; Ferreira, T.; Egger, G.; Martinez-Capel, F.; Garófano-Gómez, V.; Andrés Doménech, I.... (2013). Implementing a dynamic riparian vegetation model in three European river systems. Ecohydrology. 6(4):635-651. https://doi.org/10.1002/eco.1331S6356516

Mental health of refugees following state-sponsored repatriation from Germany

von Lersner U, Elbert T, Neuner F. Mental health of refugees following state-sponsored repatriation from Germany. BMC Psychiatry. 2008;8(1): 88.BACKGROUND: In recent years, Voluntary Assisted Return Programmes (VARPs) have received increasing funding as a potential way of reducing the number of refugees in EU member states. A number of factors may affect the mental well-being of returnees. These include adjustment to the home country following return, difficult living conditions, and long-term effects resulting from the severe traumatic stress that had originally driven the affected out of their homes. Little is known about the extent to which these and other factors may promote or inhibit the willingness of refugees to return to their country of origin. The present pilot study investigated refugees who returned to their country of origin after having lived in exile in Germany for some 13 years. METHODS: Forty-seven VARP participants were interviewed concerning their present living conditions, their views of their native country, and their attitudes towards a potential return prior to actually returning. 33 participants were interviewed nine months after returning to their country of origin. Mental health and well-being were assessed using the questionnaires Posttraumatic Stress Diagnostic Scale (PDS) and EUROHIS and the structured Mini International Neuropsychiatric Interview (M.I.N.I.).Our objectives were to examine the mental health status of refugees returning to their home country following an extended period of exile. We also aimed to assess the circumstances under which people decided to return, the current living conditions in their home country, and retrospective returnee evaluations of their decision to accept assisted return. RESULTS: Prior to returning to their home country, participants showed a prevalence rate of 53% for psychiatric disorders. After returning, this rate increased to a sizeable 88%. Substantial correlations were found between the living situation in Germany, the disposition to return, and mental health. For two thirds of the participants, the decision to return was not voluntary. CONCLUSION: Psychological strain among study participants was of a considerable magnitude. As a result of traumatic stress experienced during war and refuge, victims were vulnerable and not well equipped to cope with either post-migration stressors in exile or with a return to their country of origin. It is noteworthy that the majority returned under pressure from immigration authorities. Living conditions after return (such as housing, work, and health care) were poor and unstable. Participants also had great difficulty readapting to the cultural environment after having lived abroad for an average of 13 years. Current VARPs do not take these factors into account and are therefore not able to assist in a humanitarian reintegration of voluntary returnees

E4 ligase–specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis

Multiple protein ubiquitination events at DNA double-strand breaks (DSBs) regulate damage recognition, signaling and repair. It has remained poorly understood how the repair process of DSBs is coordinated with the apoptotic response. Here, we identified the E4 ubiquitin ligase UFD-2 as a mediator of DNA-damage-induced apoptosis in a genetic screen in Caenorhabditis elegans. We found that, after initiation of homologous recombination by RAD-51, UFD-2 forms foci that contain substrate-processivity factors including the ubiquitin-selective segregase CDC-48 (p97), the deubiquitination enzyme ATX-3 (Ataxin-3) and the proteasome. In the absence of UFD-2, RAD-51 foci persist, and DNA damage-induced apoptosis is prevented. In contrast, UFD-2 foci are retained until recombination intermediates are removed by the Holliday-junction-processing enzymes GEN-1, MUS-81 or XPF-1. Formation of UFD-2 foci also requires proapoptotic CEP-1 (p53) signaling. Our findings establish a central role of UFD-2 in the coordination between the DNA-repair process and the apoptotic response

Living Well with Diabetes: a randomized controlled trial of a telephone-delivered intervention for maintenance of weight loss, physical activity and glycaemic control in adults with type 2 diabetes

Background By 2025, it is estimated that approximately 1.8 million Australian adults (approximately 8.4% of the adult population) will have diabetes, with the majority having type 2 diabetes. Weight management via improved physical activity and diet is the cornerstone of type 2 diabetes management. However, the majority of weight loss trials in diabetes have evaluated short-term, intensive clinic-based interventions that, while producing short-term outcomes, have failed to address issues of maintenance and broad population reach. Telephone-delivered interventions have the potential to address these gaps. Methods/Design Using a two-arm randomised controlled design, this study will evaluate an 18-month, telephone-delivered, behavioural weight loss intervention focussing on physical activity, diet and behavioural therapy, versus usual care, with follow-up at 24 months. Three-hundred adult participants, aged 20-75 years, with type 2 diabetes, will be recruited from 10 general practices via electronic medical records search. The Social-Cognitive Theory driven intervention involves a six-month intensive phase (4 weekly calls and 11 fortnightly calls) and a 12-month maintenance phase (one call per month). Primary outcomes, assessed at 6, 18 and 24 months, are: weight loss, physical activity, and glycaemic control (HbA1c), with weight loss and physical activity also measured at 12 months. Incremental cost-effectiveness will also be examined. Study recruitment began in February 2009, with final data collection expected by February 2013. Discussion This is the first study to evaluate the telephone as the primary method of delivering a behavioural weight loss intervention in type 2 diabetes. The evaluation of maintenance outcomes (6 months following the end of intervention), the use of accelerometers to objectively measure physical activity, and the inclusion of a cost-effectiveness analysis will advance the science of broad reach approaches to weight control and health behaviour change, and will build the evidence base needed to advocate for the translation of this work into population health practice

Determination of nutrient salts by automatic methods both in seawater and brackish water: the phosphate blank

9 páginas, 2 tablas, 2 figurasThe main inconvenience in determining nutrients in seawater by automatic methods is simply solved: the preparation of a suitable blank which corrects the effect of the refractive index change on the recorded signal. Two procedures are proposed, one physical (a simple equation to estimate the effect) and the other chemical (removal of the dissolved phosphorus with ferric hydroxide).Support for this work came from CICYT (MAR88-0245 project) and Conselleria de Pesca de la Xunta de GaliciaPeer reviewe