Use of technical computing systems in the context of engineering problems

[EN] This paper presents a teaching innovation project based on applying technical computing systems as a resource to improve learning in the classroom and as a way of evaluating transversal competences (TC). By these means, students analyze complex kinematic and dynamic mechanical systems in the context of the subject Dynamics of Mechanical Systems of the Master’s Degree in Mechatronics Engineering at Universitat Politècnica de València (Spain). We have observed that the use of such tools improves the students learning on the contents of the subject, allows to acquire the transversal competence related to the analysis and problem solving, and enhances the ability to understand concepts intuitively. Furthermore, results clearly show a positive influence on the use of such tools for improving the professional and ethical commitment to the issues raised.Llopis Albert, C.; Rubio Montoya, FJ.; Valle-Falcones, L.; Grima-Olmedo, C. (2020). Use of technical computing systems in the context of engineering problems. Multidisciplinary Journal for Education, Social and Technological Sciences. 7(2):84-99. https://doi.org/10.4995/muse.2020.14283OJS849972Artobolevsky, I.I. 1975. Mechanisms in Modern Engineering Design: A Handbook for Engineers, Designers and Inventors. Seven books. Mir Pubblishers, Moscow.Bloom, B.S. 1956. Taxonomy of Educational Objectives: The Classification of Educational Goals. David McKay Company, p. 201-7.Llopis-Albert, C., Rubio, F., Valero, F. 2015. Improving productivity using a multi-objective optimization of robotic trajectory planning. Journal of Business Research, 68 (7), 1429-1431. https://doi.org/10.1016/j.jbusres.2015.01.027Llopis-Albert, C., Rubio, F., Valero, F. (2018). Optimization approaches for robot trajectory planning. Multidisciplinary Journal for Education, Social and Technological Sciences, 5(1), 1-16. https://doi.org/10.4995/muse.2018.9867Llopis-Albert, C., Rubio, F., Valero, F. (2019). Fuzzy-set qualitative comparative analysis applied to the design of a network flow of automated guided vehicles for improving business productivity. Journal of Business Research, 101, 737-742. https://doi.org/10.1016/j.jbusres.2018.12.076Llopis-Albert, C., Rubio, F., Valero, F., Liao, H., Zeng, S. 2019a. Stochastic inverse finite element modeling for characterization of heterogeneous material properties. Materials Research Express, 6(11), 115806. https://doi.org/10.1088/2053-1591/ab4c72Llopis-Albert, C., Valero, F., Mata, V., Pulloquinga, J.L., Zamora-Ortiz, P., Escarabajal, R.J. 2020. Optimal Reconfiguration of a Parallel Robot for Forward Singularities Avoidance in Rehabilitation Therapies. A Comparison via Different Optimization Methods. Sustainability, 12(14), 5803. https://doi.org/10.3390/su12145803Llopis-Albert, C., Valero, F., Mata, V., Zamora-Ortiz, P., Escarabajal, R.J., Pulloquinga, J.L. 2020a. Optimal Reconfiguration of a Limited Parallel Robot for Forward Singularities Avoidance. Multidisciplinary Journal for Education, Social and Technological Sciences, 7(1), 113-127. https://doi.org/10.4995/muse.2020.13352Rubio, F., Llopis-Albert, C., Valero, F., Suñer, J.L. 2015. Assembly Line Productivity Assessment by Comparing Optimization-Simulation Algorithms of Trajectory Planning for Industrial Robots. Mathematical Problems in Engineering, 10 pages. Article ID 931048. https://doi.org/10.1155/2015/931048Rubio, F., Llopis-Albert, C., Valero, F., & Suñer, J. L. 2016. Industrial robot efficient trajectory generation without collision through the evolution of the optimal trajectory. Robotics and Autonomous Systems, 86, 106-112. https://doi.org/10.1016/j.robot.2016.09.008Rubio, F., Llopis-Albert, C. 2019. Viability of using wind turbines for electricity generation in electric vehicles. Multidisciplinary Journal for Education, Social and Technological Sciences, 6(1), 115-126. https://doi.org/10.4995/muse.2019.11743Rubio, F., Valero, F., & Llopis-Albert, C. 2019a. A review of mobile robots: Concepts, methods, theoretical framework, and applications. International Journal of Advanced Robotic Systems, 16(2), 172988141983959. https://doi.org/10.1177/1729881419839596SolidWorks software. 2020. Dassault Systèmes SolidWorks Corporation. 175 Wyman Street Waltham, MA 02451, USA. https://www.solidworks.com/UPV, 2020. Proyecto institucional competencias transversales. Universitat Politècnica de València (UPV). Valencia. Spain. https://www.upv.es/entidades/ICE/info/Proyecto_Institucional_CT.pdfWolfram Mathematica software. 2020. The Wolfram Centre. Lower Road, Long Hanborough. Oxfordshire OX29 8FD, United Kingdom. https://www.wolfram.com/mathematica/Valero, F., Rubio, F., Llopis-Albert, C., Cuadrado, J.I. (2017). Influence of the Friction Coefficient on the Trajectory Performance for a Car-Like Robot. Mathematical Problems in Engineering, 9 pages. Article ID 4562647. https://doi.org/10.1155/2017/4562647Valero, F., Rubio, F., Llopis-Albert, C. 2019. Assessment of the Effect of Energy Consumption on Trajectory Improvement for a Car-like Robot. Robotica, 37(11), 1998-2009. https://doi.org/10.1017/S0263574719000407Valero, F., Rubio, F., Besa, A.J. 2019a. Efficient trajectory of a car-like mobile robot. Industrial Robot: the international journal of robotics research and application, 46(2), 211-222. https://doi.org/10.1108/IR-10-2018-021

IL-1β, TNF-α, TGF-β, and bFGF expression in bone biopsies before and after parathyroidectomy

IL-1β, TNF-α, TGF-β, and b FGF expression in bone biopsies before and after parathyroidectomy.BackgroundThere is growing evidence pointing to an involvement of cytokines and growth factors in renal osteodystrophy. In this study, the expression of interleukin-lβ (IL-1β), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), and basic fibroblast growth factor (bFGF) in bone biopsies taken from uremic patients before and 1 year after parathyroidectomy (PTX) was evaluated. Biochemical features and histomorphometric outcome were also studied.MethodsIliac bone biopsies were taken before and 1 year after PTX in nine uremic patients with severe hyperparathyroidism (HPT). Immunohistochemical techniques were used to identify the expression of IL-1β, TNF-α, TGF-β, and bFGF in these bone samples.ResultsAt the time of the second bone biopsy, the mean serum total alkaline phosphatase activity was normal, whereas mean serum intact parathyroid hormone (iPTH) level was slightly above the upper limit of normal values. Histomorphometric analysis showed a decrease in resorption parameters and static bone formation parameters after PTX. Dynamically, mineral apposition rate (MAR) and mineralization surface (MS/BS) decreased significantly. There was a marked local expression of IL-1β, TNF-α, TGF-β, and bFGF in bone biopsies before PTX, particularly in fibrous tissue and resorption areas. One year after PTX, IL-1β decreased from 23.6 ± 7.5% to 9.9 ± 3.1%, TNF-α from 4.5 ± 1.5% to 0.7 ± 0.8%, TGF-β from 49.6 ± 9.8% to 15.2 ± 4.6%, and bFGF from 50.9 ± 12.7% to 12.9 ± 7.9% (P < 0.001). A significant correlation was documented between cytokines and growth factors expression in bone with iPTH levels before and after PTX (P < 0.05).ConclusionsBased on these results, we suggest that IL-1β, TNF-α, TGF-β, and bFGF are involved in bone remodeling regulation, acting as local effectors, possibly under the control of PTH

In situ detection of AE2 anion-exchanger mRNA in the human liver

Na+-independent anion exchangers, a family of membrane proteins that mediate electroneutral exchanges of chloride and bicarbonate ions across the cell membrane, are considered to be involved in intracellular pH regulation as well as in transepithelial acid/base transport. Previous immunohistochemical data have shown that anion-exchanger-2 (AE2) protein is expressed in the liver parenchyma, localizing at both the canaliculi and the luminal surfaces of intrahepatic bile ducts, where it may have a role in the biliary secretion of bicarbonate. In the present study, we have carried out in situ hybridization experiments on biopsies of human liver using three overlapping antisense anion-exchanger-2 riboprobes. Anion-exchanger-2 mRNA signals were localized mainly in the cytoplasm of terminal and interlobular bile-duct cells, whereas weaker signals were observed in bile-duct cells of larger intrahepatic ducts. Furthermore, some hepatocytes, mostly periportal, contained detectable anion-exchanger-2 mRNA signals in their cytoplasm. No hybridization signals were observed in controls with sense riboprobes, with omission of the antisense probe, or with treatment of the sections with RNase before hybridizations. Finally, intense anion-exchanger-2 hybridization signals were observed in lymphomononuclear cells in sinusoids and in portal infiltrates. Immunocytochemical data from reverse-phase sections suggest that these cells correspond to some of the CD45R+ (UCHL1+) T lymphocytes resident in the liver

Effectiveness of XP-endo finisher in the reduction of bacterial load in oval-shaped root canals

This study investigated the effectiveness of XP-Endo Finisher (XPF) associated with XP-Endo Shaper (XPS) or Reciproc Blue (RB) files in reducing bacterial load in oval-shaped root canals (RC) during chemomechanical preparation (CMP) using 0.9% saline solution (NaCl) or 2.5% sodium hypochlorite (NaOCl). Eighty mandibular incisors with single oval-shaped RC were contaminated with Enterococcus faecalis. The teeth were randomly assigned to eight experimental groups (n = 10) according to the CMP, as follows: G1: XPS, G2: XPS + XPF, G3: RB, and G4: RB + XPF. CMP was performed with NaCl or NaOCl. The reduction of bacterial load was assessed by colony-forming unit count before (S1) and after (S2) CMP. Data normality was verified by using Shapiro-Wilk test. ANOVA, Tukey's test, and Bonferroni post-hoc test were used at a 5% significance level. Culturable bacteria were present in all S1 samples (p>0.05). All instrumentation techniques were effective in reducing bacterial load, irrespective of the irrigating solution (p < 0.05). With the use of NaCl, RB was more effective than XPS (p = 0.035). With the use of NaOCl, XPS and RB presented similar effectiveness (p = 0.779). XPF enhanced the bacterial reduction of both systems tested (p < 0.05). The use of NaOCl improved the CMP, irrespective of the instrumentation technique used (p < 0.05). In conclusion, XPS and RB files are effective in reducing bacterial levels in oval-shaped RC. The use of XPF as a method of agitation of the irrigating solution improved the cleaning efficiency of both file systems tested. Mechanical preparation performed with saline solution decreased culturable bacteria from the root canal, but antimicrobial substances such as NaOCl should be used to achieve a significantly better disinfection33CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP308162/2014-5não tem2015/23479-

Luxación recidivante de prótesis total de cadera

Más del 50% de los casos de caderas inestables se pueden resolver mediante la reducción cerrada de la luxación. Un intento sistemático para entender la causa de la inestabilidad, normalmente suele conducir a un tratamiento eficaz del problema cuando hace falta cirugía. Los componentes constreñidos o de retención han mejorado las posibilidades de resolver los problemas difíciles de inestabilidad, pero los efectos negativos potenciales de estos componentes deben tenerse también en cuenta. Los implantes tripolares, cuyos resultados clínicos en la literatura son muy alentadores, ocupan un lugar de elección en el arsenal terapéutico de la inestabilidad protética

Effect of serum phosphate on parathyroid hormone secretion during hemodialysis

Effect of serum phosphate on parathyroid hormone secretion during hemodialysis.BackgroundRecent studies have demonstrated that a high concentration of phosphate directly stimulates parathyroid hormone (PTH) secretion. High serum levels of phosphate are usually observed in patients with end-stage renal disease. The aim of the present study was to evaluate whether serum phosphate concentration had an acute effect on PTH secretion in hemodialysis patients. The levels of serum phosphate were manipulated during the hemodialysis session by using a phosphate free dialysate or a dialysate with a high content of phosphate.MethodsTen stable hemodialysis patients with PTH values above 300 pg/ml were included in the study. A PTH-calcium curve was obtained during both high phosphate and phosphate free hemodialysis.ResultsThe serum phosphate concentration remained high (2.17 ± 0.18mM) throughout the high phosphate hemodialysis and decreased progressively to normal levels (1.02 ± 0.06mM) during the phosphate free hemodialysis. The serum PTH levels at maximal inhibition by hypercalcemia (minimal PTH) were greater during the high phosphate than the phosphate free hemodialysis (413 ± 79 vs. 318 ± 76 pg/ml, P < 0.003). In all patients the values of minimum PTH were greater during the high phosphorus than the phosphorus free hemodialysis. The values of maximally stimulated PTH during hypocalcemia and the set point of the PTH-calcium curve were similar during the high phosphate and the phosphate free hemodialysis.ConclusionThe maintenance of high serum phosphorus levels during hemodialysis prevented, in part, the inhibition of PTH secretion by calcium, which strongly suggests that in hemodialysis patients high serum phosphate contributes directly to the elevation of PTH levels despite normal or high serum calcium concentration

Revista de Vertebrados de la Estación Biológica de Doñana

Dimorfismo sexual en Microtus cabrerae en base a los caracteres de su pelvis.Notas sobre la distribución y ecología de Microtus cabreae, Thomas, 1906.Alimentación de la culebra bastarda (Malpolon monspessulanus, Ophidia, Colubridae) en el S. O. de España.Selectividad en la predación de la lechuza común (Tyto alba) sobre Rano ridibunda.Variations in the food habits of the european Eagle Owl. (Bubo bubo)Contaminación en huevos de aves silvestres de lSuroeste de España por residuos organoclorados (Insecticidas y bifenilos policlorados)Sobre el status taxonómico del águila imperial ibéricaEstudio filogenético y comparativo de Microtus cabrerae y Microtus brecciensisDescripción de una nueva especie de liebre (Lepus castroviejoi), endémica de la Cordillera CantábricaPeer reviewe

A Community Program of Integrated Care for Frail Older Adults: +AGIL Barcelona

Objectives: To assess the 3-month impact on physical function of a program for community-dwelling frail older adults, based on the integration of primary care, geriatric medicine, and community resources, implemented in 'real life'. Design: Interventional cohort study. Setting: Primary care in Barcelona, Spain. Participants: Individuals aged ≥80 years (n=134), presenting at least one sign of frailty (i.e., slow gait speed, weakness, memory complaints, involuntary weight loss, poor social support). Intervention: After frailty screening by the primary care team, candidates were referred to a geriatric team (geriatrician + physical therapist), who performed a comprehensive geriatric assessment and designed a tailored multidisciplinary intervention in the community, including a) multi-modal physical activity (PA) sessions, b) promotion of adherence to a Mediterranean diet c) health education and d) medication review. Measurements: Participants were assessed based on a comprehensive geriatric assessment including physical performance (Short Physical Performance Battery -SPPB- and gait speed), at baseline and at a three month follow-up. Results: A total of 112 (83.6%) participants (mean age=80.8 years, 67.9% women) were included in this research. Despite being independent in daily life, participants' physical performance was impaired (SPPB=7.5, SD=2.1, gait speed=0.71, SD=0.20 m/sec). After three months, 90.2% of participants completed ≥7.5 physical activity sessions. The mean improvements were +1.47 (SD 1.64) points (p<0.001) for SPPB, +0.08 (SD 0.13) m/sec (p<0.001) for gait speed, -5.5 (SD 12.10) sec (p<0.001) for chair stand test, and 53% (p<0.001) improved their balance. Results remained substantially unchanged after stratifying the analyses according to the severity of frailty. Conclusions: Our results suggested that a 'real-world' multidisciplinary intervention, integrating primary care, geriatric care, and community services may improve physical function, a marker of frailty, within 3 months. Further studies are needed to address the long-term impact and scalability of this implementation program

Robustness of dead Cas9 activators in human pluripotent and mesenchymal stem cells

Human pluripotent stem cells (hPSCs) and mesenchymal stromal/stem cells (hMSCs) are clinically relevant sources for cellular therapies and for modeling human development and disease. Many stem cell-based applications rely on the ability to activate several endogenous genes simultaneously to modify cell fate. However, genetic intervention of these cells remains challenging. Several catalytically dead Cas9 (dCas9) proteins fused to distinct activation domains can modulate gene expression when directed to their regulatory regions by a specific single-guide RNA (sgRNA). In this study, we have compared the ability of the first-generation dCas9-VP64 activator and the second-generation systems, dCas9-SAM and dCas9-SunTag, to induce gene expression in hPSCs and hMSCs. Several stem cell lines were tested for single and multiplexed gene activation. When the activation of several genes was compared, all three systems induced specific and potent gene expression in both single and multiplexed settings, but the dCas9-SAM and dCas9-SunTag systems resulted in the highest and most consistent level of gene expression. Simultaneous targeting of the same gene with multiple sgRNAs did not result in additive levels of gene expression in hPSCs nor hMSCs. We demonstrate the robustness and specificity of second-generation dCas9 activators as tools to simultaneously activate several endogenous genes in clinically relevant human stem cells.We thank CERCA/Generalitat de Catalunya and Fundació Josep Carreras-Obra Social la Caixa for their institutional support. We thank Jose Luis Sardina (IJC, Barcelona) for technical assistance with the teratoma assays. Financial support for this work was obtained from the Catalunya Goverment (SGR330 and PERIS 2017-2019), the Spanish Ministry of Economy and Competitiveness (SAF2016-80481-R), the European Research Council (CoG-2014-646903), and the Fundación Leo Messi to P.M.; the Spanish Association against Cancer (AECC-CI-2015) and the Health Institute Carlos III (ISCIII/FEDER, PI17/01028) to C.B.; the Biotechnology and Biological Sciences Research Council (BBRSC) to L.M.F. and A.F.; and the Spanish National Research and Development Plan (ISCIII/FEDER, PI17/02303) and the AEI/MICIU EXPLORA Project (BIO2017-91272-EXP) to S.R.-P. P.M. is an investigator of the Spanish Cell Therapy Cooperative Network (TERCEL). R.T.-R. is supported by a postdoctoral fellowship from the Asociación Española Contra el Cáncer (AECC).S

Revista de Vertebrados de la Estación Biológica de Doñana

Descripción de una nueva subespecie de lagarto ágil (Lacerta agilis garzoni) de los PirineosDiet of the Montagu's Harrier (Circus pygargus) in southwestern Spain SpainObservaciones ornitológicas en la Guayana francesaDaily feeding rhythm of ducks on the marismas of the Guadalquivir and their responses to birds of preyA note on the emetic technique for obtaining food samples from passerine birds.Distribución de contaminantes organoclorados en tejidos de garza imperial (Ardea purpurea) y pato cuchara (Anas clypeata) de la Reserva Biológica de Doñana.Etograma cuantificado del gamo (Dama dama) en libertad.Peer reviewe