Entornos virtuales en asignaturas del área de las tecnologías educativas: estudio de casos múltiples y marco de análisis TPACK

Comunicamos resultados seleccionados de una investigación en torno del Conocimiento Didáctico Tecnológico del Contenido en docentes universitarios, específicamente sobre los niveles de integración curricular de las TIC y su articulación a procesos de innovación didáctica en tres materias vinculadas al campo de las Tecnologías Educativas. Recientemente Koehler, Mishra y Cain aportan la dimensión tecnológica al Conocimiento Didáctico del Contenido, programa iniciado por Shulman a mediados de la decada del 80, cuya incorporación en las aulas universitarias supone considerar su potencialidad en la transformación del contenido de enseñanza, la cognición, entre otros. Adoptamos la estrategia de estudios de casos múltiples, a los que construimos con narrativas docentes y Entornos Virtuales de Enseñanza y Aprendizaje. Las primeras aproximaciones ponen en evidencia que en la mayoría de los proyectos se otorga a las TIC un nivel de uso en el que el aprendizaje y la cognición tienen un papel periférico. Uno, muestra un nivel de integración curricular con visibilidad en el aprender, donde el entorno virtual constituye un potenciador de la propuesta pedagógico-didáctica en lugar de banalizarla, pues los contextos de uso de las TIC generan dinámicas de innovación y mejora en los procesos de enseñanza y aprendizaje.We communicate preliminary results of an investigation around the Didactic Technological Knowledge of the Content in university teachers, specifically on the levels of ICT curricular integration and its articulation to processes of didactic innovation in three subjects related to the field of Educational Technologies. Recently Koehler, Mishra and Cain provide the technological dimension to the Didactic Knowledge of Content, program initiated by Shulman in the mid-1980s, whose incorporation in the university classrooms means considering its potential in the transformation of teaching content, cognition, among others. We adopted the multi-case study strategy, to those we build with teaching narratives and Virtual Environments of Teaching and Learning. The first approximations show that in most projects ICT is given a level of use in which learning and cognition have a peripheral role. One, shows a level of curricular integration with visibility in learning, where the virtual environment is an enhancer of the pedagogical-didactic proposal instead of trivializing it, since the contexts of ICT use generate dynamics of innovation and improvement in the teaching and learning processes.Fil: Flores, Fernando Agustin Santiago. Universidad Nacional del Nordeste. Facultad de Humanidades. Instituto de Ciencias de la Educación; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentin

El modelo Tpack en la praxis docente en una universidad argentina: conocimientos y prácticas docentes en torno al conocimiento didáctico-tecnológico del contenido (CDTC) en aulas universitarias

Presentamos una aproximación a los Conocimientos y prácticas docentes en torno al Conocimiento Didáctico-Tecnológico del Contenido (CDTC) o Technological Pedagogical Content Knowledge (TPACK) en aulas universitarias de carreras humanísticas en una universidad argentina.Metodológicamente, construimos un caso múltiple constituido por equipos docentes de seis asignaturas vinculadas con Tecnología educativa de Profesorados y Licenciaturas de una de las Unidades Académicas. También, realizamos una doble triangulación: intra-metodológica (fuentes primarias y secundarias) y de investigadores y docentes participantes como estrategia de validación pertinente en un estudio cualitativo.Encontramos que las maneras de abordar la relación teoría-práctica devienen envariadas configuraciones del CDTC, según las trayectorias profesionales de las profesoras y entramados de experiencias de formación permanente, gestión e investigación. Configuraciones construidas individualmente y re-construidas colectivamente en los territorios disciplinaresacadémicos y diferentes contextos.act We present an approach to the Knowledge and teaching practices around the Technological Pedagogical Content Knowledge (TPACK) or Conocimiento Didáctico-Tecnológico del Contenido (CDTC) in university classrooms of humanistic careers in an Argentine university. Methodologically, we built a multiple case consisting of teaching teams of six subjects linked to the Educational Technology of Teachers and Degrees from one of the Academic Units. Also, we perform a double triangulation: intra - methodological (primary and secondary sources) and of participating researchers and teachers as a relevant validation strategy in a qualitative study. We find that the ways of approaching the theory-practice relationship come in various configurations of the TPACK, according to the professional trajectories of the teachers and networks of experiences of permanent training, management and research. Configurations built individually and collectively re-built in the disciplinary-academic territories and different contexts.Fil: Flores, Fernando Agustin Santiago. Universidad Nacional del Nordeste. Facultad de Humanidades. Instituto de Ciencias de la Educación; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Ortiz, Margarita Cristina. Universidad Nacional del Nordeste. Facultad de Humanidades. Instituto de Ciencias de la Educación; Argentin

Contextual aspects in the development of professional knowledge of teachers: Study of cases

Sintetizamos avances de una investigación en curso, cuyo objetivo apunta al análisis de la dimensión tecnológica en el conocimiento profesional del profesorado de bachillerato. El estudio integra el Programa de Investigación: Conocimiento y Formación Docente (CyFoD), radicado en una universidad pública de la región nordeste de Argentina. En esta ocasión, analizamos lineamientos de políticas educativas que aparecieron en la última década con el propósito de reorganizar la enseñanza secundaria y mejorar sus condiciones pedagógicas y tecnológicas. La estrategia metodológica es el estudio de caso. Su armado se realiza a partir de triangular entrevistas semiestructuradas, observación no participante de clases y análisis documental; su validación surge de procesos recursivos mediante construcciones narrativas. Aunque nunca antes se hizo un uso tan extensivo, variado e impuesto de las TIC, la frontera entre su uso e integración curricular devela una aguda tensión, su debate cobra un nuevo impulso y trasciende la coyuntura pandémica.We synthesize advances of an ongoing investigation, whose objective points to the analysis of the technological dimension in the professional knowledge of high school teachers. The study is part of the Research Program: Knowledge and Teacher Training (CyFoD), based in a public university in the northeast region of Argentina. On this occasion, we analyze educational policy guidelines that appeared in the last decade with the purpose of reorganizing secondary education and improving its pedagogical and technological conditions. The methodological strategy is the case study. Its assembly is carried out from semi-structured triangular interviews, non-participant observation of classes and documentary analysis; its validation arises from recursive processes through narrative constructions. Although never before has such an extensive, varied and imposed use of ICT been made, the border between its use and curricular integration reveals an acute tension, its debate gains new momentum and transcends the pandemic situation.Fil: Flores, Fernando Agustin Santiago. Universidad Nacional del Nordeste. Facultad de Humanidades. Instituto de Ciencias de la Educación; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Chan- Te- Nez, Alejandro Fabián. Universidad Nacional del Nordeste. Facultad de Humanidades. Instituto de Ciencias de la Educación; Argentin

A produção de materiais didáticos a partir de perspectivas convergentes: Design Gráfico e TPCK

We present selected results of a recently developed study, carried out within the framework of a research program on knowledge and teacher training, in a public university in the northeast of Argentina (NEA Geographic region); This time we examine the configuration of the content technological didactic knowledge (CDTC) of an experienced teacher in the area of ​​Educational Technology of an academic unit, when teaching how to produce ICT-mediated didactic materials. The TPCK model and conceptual contributions from the Graphic Design area on visual communication are used as a reference for analysis. The methodological strategy is the single case study with a holistic approach. The narrative construction of the case is supported by the triangulation of different sources and data collection instruments. The results reveal that the perspective of graphic design substantially improves the quality of the didactic material, although its functionality in the pedagogical proposal is subject to the resulting framework of all the components of the TPCK model.Presentamos resultados seleccionados de un estudio desarrollado recientemente, realizado en el marco de un programa de investigación sobre conocimiento y formación docente, en una universidad pública del nordeste argentino (región geográfica NEA); en esta ocasión examinamos la configuración del conocimiento didáctico tecnológico del contenido (CDTC) de una docente experimentada del área de Tecnología educativa de una unidad académica, al enseñar a producir materiales didácticos mediados por TIC. Se utiliza como referencia de análisis el modelo TPCK y aportes conceptuales del área del Diseño Gráfico sobre comunicación visual. La estrategia metodológica es el estudio de caso único de enfoque holístico. La construcción narrativa del caso se apoya en la triangulación de distintas fuentes e instrumentos de recolección de datos. Los resultados revelan que la perspectiva del diseño gráfico mejora sustancialmente la calidad del material didáctico aunque su funcionalidad en la propuesta pedagógica queda supeditada al entramado resultante de todos los componentes del modelo TPCK.Apresentamos resultados selecionados de um estudo recentemente desenvolvido, realizada no âmbito de um programa de pesquisa sobre formação e conhecimento docente, em uma universidade pública do nordeste argentino (região geográfica NEA); na ocasião, examinamos a configuração do conteúdo tecnológico didático conhecimento (CDTC) de um professor experiente na área de tecnologia educacional uma unidade acadêmica, ao ensinar como produzir materiais didáticos mediados por TIC. O modelo TPCK e as contribuições conceituais da área de Design Gráfico sobre comunicação visual são usados ​​como referência para análise. A estratégia metodológica é o estudo de caso único com abordagem holística. A construção narrativa do caso é sustentada pela triangulação de diferentes fontes e instrumentos de coleta de dados. Os resultados revelam que a perspectiva do design gráfico melhora substancialmente a qualidade do material didático, embora sua funcionalidade na proposta pedagógica esteja sujeita ao enquadramento resultante de todos os componentes do modelo TPCK

A produção de materiais didáticos a partir de perspectivas convergentes: Design Gráfico e TPCK

We present selected results of a recently developed study, carried out within the framework of a research program on knowledge and teacher training, in a public university in the northeast of Argentina (NEA Geographic region); This time we examine the configuration of the content technological didactic knowledge (CDTC) of an experienced teacher in the area of ​​Educational Technology of an academic unit, when teaching how to produce ICT-mediated didactic materials. The TPCK model and conceptual contributions from the Graphic Design area on visual communication are used as a reference for analysis. The methodological strategy is the single case study with a holistic approach. The narrative construction of the case is supported by the triangulation of different sources and data collection instruments. The results reveal that the perspective of graphic design substantially improves the quality of the didactic material, although its functionality in the pedagogical proposal is subject to the resulting framework of all the components of the TPCK model.Presentamos resultados seleccionados de un estudio desarrollado recientemente, realizado en el marco de un programa de investigación sobre conocimiento y formación docente, en una universidad pública del nordeste argentino (región geográfica NEA); en esta ocasión examinamos la configuración del conocimiento didáctico tecnológico del contenido (CDTC) de una docente experimentada del área de Tecnología educativa de una unidad académica, al enseñar a producir materiales didácticos mediados por TIC. Se utiliza como referencia de análisis el modelo TPCK y aportes conceptuales del área del Diseño Gráfico sobre comunicación visual. La estrategia metodológica es el estudio de caso único de enfoque holístico. La construcción narrativa del caso se apoya en la triangulación de distintas fuentes e instrumentos de recolección de datos. Los resultados revelan que la perspectiva del diseño gráfico mejora sustancialmente la calidad del material didáctico aunque su funcionalidad en la propuesta pedagógica queda supeditada al entramado resultante de todos los componentes del modelo TPCK.Apresentamos resultados selecionados de um estudo recentemente desenvolvido, realizada no âmbito de um programa de pesquisa sobre formação e conhecimento docente, em uma universidade pública do nordeste argentino (região geográfica NEA); na ocasião, examinamos a configuração do conteúdo tecnológico didático conhecimento (CDTC) de um professor experiente na área de tecnologia educacional uma unidade acadêmica, ao ensinar como produzir materiais didáticos mediados por TIC. O modelo TPCK e as contribuições conceituais da área de Design Gráfico sobre comunicação visual são usados ​​como referência para análise. A estratégia metodológica é o estudo de caso único com abordagem holística. A construção narrativa do caso é sustentada pela triangulação de diferentes fontes e instrumentos de coleta de dados. Os resultados revelam que a perspectiva do design gráfico melhora substancialmente a qualidade do material didático, embora sua funcionalidade na proposta pedagógica esteja sujeita ao enquadramento resultante de todos os componentes do modelo TPCK

The technological didactic knowledge of the content (TPACK) in university teachers: images and modes of expression in classroom practices

Presentamos resultados preliminares en el marco del proyecto de investigación: “La construcción del conocimiento didáctico del contenido en profesores experimentados y principiantes de la Universidad Nacional del Nordeste. Estudio de casos múltiples”; específicamente en torno a las visiones que del Conocimiento Didáctico Tecnológico del Contenido sustentan profesores/as y sus modos complejos de manifestarse en las prácticas de asignaturas de Profesorados y Licenciaturas de la Facultad de Humanidades. En esta oportunidad, ubicamos el análisis en las imágenes acerca del uso de las tecnologías en la enseñanza y su incorporación efectiva a un ciclo didáctico concreto. La propuesta teórica que asumimos es el Modelo CDTC (“Conocimiento Didáctico Tecnológico del Contenido”). Adoptamos como estrategia metodológica al estudio de casos múltiples, para su construcción empleamos la revisión y análisis de documentos profesionales (CV y planificaciones), entrevistas semi estructuradas y observación no participante de Clases. Los casos están constituidos por docentes de tres (3) asignaturas. El predominio de uso de las tecnologías asimilativas (apoyo a la exposición), da cuenta de una cultura profesional que aún no ha logrado internalizar el modelo CDTC en la unidad académica.We present preliminary results in the framework of the research project: "The construction of the didactic knowledge of content in experienced teachers and beginners of the National University of the Northeast. Multiple case study "; specifically around the visions that of the Technological Didactic Knowledge of the Content support professors and their complex ways of manifesting in the practices of subjects of Teachers and Degrees of the Faculty of Humanities. In this opportunity, we place the analysis in the images about the use of technologies in teaching and its effective incorporation into a specific didactic cycle. The theoretical proposal that we assume is the Model TPACK (“Technological Pedagogical Content Knowledge”). We adopt as a methodological strategy the study of multiple cases, for its construction we use the review and analysis of professional documents (CV and schedules), semi-structured interviews and non-participant observation of classes. The cases are constituted by teachers of three (3) subjects. The predominance of the use of assimilative technologies (support for exposure), it shows a professional culture that has not yet managed to internalize the TPACK model in the academic unit.Fil: Flores, Fernando Agustin Santiago. Universidad Nacional del Nordeste. Facultad de Humanidades. Instituto de Ciencias de la Educación; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Ortiz, Margarita Cristina. Universidad Nacional del Nordeste. Facultad de Humanidades. Instituto de Ciencias de la Educación; Argentin

TPACK: a model to analyze university teaching practices: the case of an expert teacher

En este artículo analizamos desde las dimensiones del modelo TPACK la praxis docente de una profesora de la asignatura Educación a Distancia de la Licenciatura en Ciencias de la Educación en el marco del proyecto: La construcción del Conocimiento Didáctico del Contenido en profesores experimentados y principiantes de una universidad del nordeste argentino. Estudio de casos múltiples.Con una perspectiva cualitativa, construimos un caso narrativo, a partir de la doble triangulación: a) de fuentes primarias- observaciones y entrevistas-,secundarias -análisis de documentos personales y curriculares-; y b) de las interpretaciones de la docente e investigadores; con el objetivo de elucidar dimensiones del TPACK en clases presenciales y entornos virtuales.Organizamos los resultados según las categorías emergentes que se detallan: status del TPACK en el conocimiento profesional docente; auto-percepción acerca del uso de las nuevas tecnologías en la asignatura; tipos y frecuencia de uso de las TIC en la enseñanza; visiones sobre los modos de integración del constructo TPACK en la praxis; características centrales de las propuestas de enseñanza y aprendizaje con tecnologías; manifestaciones del TPACK en un ciclo didáctico de la asignatura.A partir del cruce y convergencia de estas categorías y de la trayectoria y posición de la profesora en la función docente, abordamos el entramado de saberes y prácticas con características propias de la experticia docente a partir del TPACK.In this article we analyze the teaching practice of a teacher of the subject Distance Education of the Degree in Educational Sciences, from the dimensions of the TPACK model. The framework of the article is the project: “The construction of the Pedagogical Content Knowledge in experienced and beginners teachers of the Universidad Nacional del Nordeste in Argentina. Multiple case study”.With a qualitative perspective, we build a narrative case, based on double triangulation: a) from primary sources-observations and interviews-, secondary -analysis of personal and curricular documents-; and b) the interpretations of the teacher and researchers; with the objective of identifying TPACK dimensions in face-to-face classes and virtual environments.We organize the results according to the emerging categories that are detailed: status of the TPACK in the professional knowledge of teachers; self-perception about the use of new technologies in the subject; types and frequency of use of new technologies in education; visions on the integration modes of the TPACK construct in praxis; main characteristics of the teaching and learning proposals with technologies; TPACK manifestations in the pedagogical cycle of the subject.From the convergence of these categories and the teacher’s career and position in the teaching role, we tackled the framework of knowledge and practices with characteristics of the teaching expertise from the TPACK.Fil: Flores, Fernando Agustin Santiago. Universidad Nacional del Nordeste. Facultad de Humanidades. Instituto de Ciencias de la Educación; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Ortiz, Margarita Cristina. Universidad Nacional del Nordeste. Facultad de Humanidades. Instituto de Ciencias de la Educación; ArgentinaFil: Buontempo, María Paula. Universidad Nacional del Nordeste. Facultad de Humanidades. Instituto de Ciencias de la Educación; Argentin

7th Drug hypersensitivity meeting: part two

Table of contents Poster walk 11: miscellaneous drug hypersensitivity 2 (P92–P94, P96–P101) P92 16 years of experience with proton pump inhibitors (PPIs) Javier Dionicio Elera, Cosmin Boteanu, Maria Aranzazu Jimenez Blanco, Rosario Gonzalez-Mendiola, Irene Carrasco García, Antonio Alvarez, Jose Julio Laguna Martinez P93 Allergy evaluation of quinolone induced adverse reactions Jaume Martí Garrido, Carla Torán Barona, Carolina Perales Chorda, Ramón López Salgueiro, Miguel Díaz Palacios, Dolores Hernández Fernández De Rojas P94 Bupropion-induced acute urticaria and angioedema, a case report Emre Ali Acar, Ayse Aktas, Aylin Türel Ermertcan, Peyker Temiz P96 Delayed type hypersensitivity and study of cross-reactivity between proton-pump inhibitors Chien-Yio Lin, Chung-Yee Rosaline Hui, Ya-Ching Chang, Chih-Hsun Yang, Wen-Hung Chung P97 Diagnostic work-up in suspected hypersensitivity to proton-pump inhibitors: looking at cross-reactivity Fabrícia Carolino, Diana Silva, Eunice Dias De Castro, Josefina R. Cernadas P98 Management of infusion-related hypersensitivity reactions to enzyme replacement therapy for lysosomal diseases Luis Felipe Ensina, Carolina Aranda, Ines Camelo Nunes, Alex Lacerda, Ana Maria Martins, Ekaterini Goudouris, Marcia Ribeiro, José Francisco Da Silva Franco, Leandra Queiroz, Dirceu Solé P99 Management of insulin allergy with continuous subcutaneous insulin infusion Ceyda Tunakan Dalgiç, Aytül Zerrin Sin, Fatma Düsünür Günsen, Gökten Bulut, Fatma Ömür Ardeniz, Okan Gülbahar, Emine Nihal Mete Gökmen, Ali Kokuludag P100 Off-label use of icatibant for management of serious angioedema associated with angiotensin inhibitors Ana M. Montoro De Francisco, Talía Mª De Vicente Jiménez, Adriana M. Mendoza Parra, Angella M. Burgos Pimentel, Amelia García Luque P101 Thiocolchicoside anaphylaxis: an unusual suspect? Luis Amaral, Fabricia Carolino, Leonor Carneiro Leão, Eunice Castro, Josefina Cernadas Poster walk 12: betalactam hypersensitivity (P102–P111) P102 A curious delayed reading: a case report of a β-lactam allergy in a child Nicole Pinto, Joana Belo, João Marques, Pedro Carreiro-Martins, Paula Leiria-Pinto P103 Betalactam-induced hypersensitivity: a 10-years’ experience Amel Chaabane, Haifa Ben Romdhane, Nadia Ben Fredj, Zohra Chadly, Naceur A. Boughattas, Karim Aouam P104 Cefazolin hypersensitivity: towards optimized diagnosis Astrid P. Uyttebroek, Chris H. Bridts, Antonino Romano, Didier G. Ebo, Vito Sabato P105 Clavulanic acid allergy: two cases report Anabela Lopes, Joana Cosme, Rita Aguiar, Tatiana Lourenço, Maria-João Paes, Amélia Spínola-Santos, Manuel Pereira-Barbosa P106 Diagnosis of betalactam allergy in an allergy department Cíntia Rito Cruz, Rute Pereira Dos Reis, Elza Tomaz, Ana Paula Pires, Filipe Inácio P107 Diagnostic work-up of 410 patients with suspicion of betalactam antibiotic hypersensitivity Filipe Benito-Garcia, Inês Mota, Magna Correia, Ângela Gaspar, Marta Chambel, Susana Piedade, Mário Morais-Almeida P108 Immediate selective hypersensitivity reactions to clavulanic acid Alla Nakonechna, Yurij Antipkin, Tetiana Umanets, Fernando Pineda, Francisca Arribas, Volodymyr Lapshyn P109 Prevalence and incidence of penicillin hypersensitivity reactions in Colombia Pablo Andrés Miranda, Bautista De La Cruz Hoyos P110 Selective sensitization to amoxicilin and clavulanic acid Jose Julio Laguna Martinez, Aranzazu Jimenez Blanco, Javier Dionicio Elera, Cosmin Boteanu, Rosario Gonzalez-Mendiola, Marta Del Pozo P111 Infliximab-specific T cells are detectable also in treated patients who have not developed anti-drug antibodies Alessandra Vultaggio, Francesca Nencini, Sara Pratesi, Andrea Matucci, Enrico Maggi Poster walk 13: biologicals, local anesthetics, others (P112–P118) P112 A case report of allergic immediate systemic reaction to adalimumab and certolizumab Ceyda Tunakan Dalgiç, Fatma Düsünür Günsen, Gökten Bulut, Fatma Ömür Ardeniz, Okan Gülbahar, Emine Nihal Mete Gökmen, Aytül Zerrin Sin, Ali Kokuludag P113 Allergy to local anesthetics: negative predictive value of skin tests Ivana Cegec, Danica Juricic Nahal, Viktorija Erdeljic Turk, Matea Radacic Aumiler, Ksenija Makar Ausperger, Iva Kraljickovic, Iveta Simic P114 Cutaneous adverse reactions of molecular targeted agents: a retrospective analysis in 150 patients in our department Yukie Yamaguchi, Tomoya Watanabe, Megumi Satoh, Tomohiko Tanegashima, Kayoko Oda, Hidefumi Wada, Michiko Aihara P115 Generalized paralysis induced by local lidocaine injection Jaechun Jason Lee, Jay Chol Choi, Hwa Young Lee P116 Hypersensitivity to local anaesthetics: a 10 year review Rosa-Anita Rodrigues Fernandes, Emília Faria, Joana Pita, Nuno Sousa, Carmelita Ribeiro, Isabel Carrapatoso, Ana Todo Bom P117 Local anaesthetics: a rare culprit in hypersensitivity reactions Ana Rodolfo, Eunice Dias-Castro, Josefina Cernadas P118 Stevens–Johnson syndrome in clinical practice: a variant of clinical course Marina Voronova Poster walk 14: RCM (P119–P128) P119 13 cases of severe anaphylactic reactions due to radiocontrast media Jaume Martí Garrido, Ramon Lopez Salgueiro, Diana Kury Valle, Verónica Pacheco Coronel, Carolina Perales Chordá, Dolores Hernandez Fernandez De Rojas P120 Anaphylactic shock after administration of iodinated contrast medium during cardiac catheterization Roselle Catherine Yu Madamba, Marta Ferrer, Maria Jose Goikoetxea, Carmen D’Amelio, Amalia Bernad, Olga Vega, Gabriel Gastaminza P121 Anaphylactic shock and cardiac arrest induced by gadolinium-based contrast agents Beatriz Pola Bibián, Marina Lluncor Salazar, Gemma Vilà Nadal, Ana María Fiandor Roman, Javier Dominguez Ortega, Miguel Gonzalez Muñoz, Santiago Quirce Gancedo, Maria Rosario Cabañas Moreno P122 Anaphylaxis to gadobenate and cross-reactivity to other gadolinium-based contrast agents in two patients Kathrin Scherer Hofmeier P123 Anaphylaxis to glatiramer acetate in a patient with multiple sclerosis Fabrícia Carolino, Vladyslava Barzylovych, Josefina R. Cernadas P124 Delayed hypersensitivity reaction to radiocontrast media Fabrícia Carolino, Diana Silva, Leonor Leão, Josefina R. Cernadas P125 Drug reaction with eosinophilia and systemic symptoms induced by iodixanol Gemma Vilà-Nadal, Beatriz Pola, Marina Lluncor, Ana Fiandor, Teresa Bellón, Javier Domínguez, Santiago Quirce P126 Electronic consultation support system for radiocontrast media hypersensitivity changes clinician’s behavior Min-Suk Yang, Sun-Sin Kim, Sae-Hoon Kim, Hye-Ryun Kang, Heung-Woo Park, Sang-Heon Cho, Kyung-Up Min, Yoon-Seok Chang P127 Hypersensitivity reactions to iodinated contrast media: skin testing and follow-up Danica Juricic Nahal, Ivana Cegec, Viktorija Erdeljic Turk, Iva Kraljickovic, Matea Radacic Aumiler, Ksenija Makar Ausperger, Iveta Simic P128 Would iodine allergy exist? Clémence Delahaye, Jenny Flabbee, Julie Waton, Olivia Bauvin, Annick Barbaud Poster walk 15: MPE/type 4 (P129–P137) P129 Delayed hypersensitivity cutaneous reactions: a case/control study from a tunisian database Karim Aouam, Najah Ben Fadhel, Zohra Chadly, Nadia Ben Fredj, Naceur A. Boughattas, Amel Chaabane P130 Delayed hypersensitivity reactions to cephalosporins: a review of seven cases Joana Cosme, Anabela Lopes, Amélia Spínola-Santos, Manuel Pereira-Barbosa P131 Diclofenac induced allergic contact dermatitis: case series of four patients Sandra Jerkovic Gulin, Anca Chiriac P132 Late-onset maculopapular rash to irbesartan Bárbara Kong Cardoso, Elza Tomaz, Regina Viseu, Filipe Inácio P133 Nonimmediate hypersensitivity reactions to betalactams: a retrospective analysis Ana Moreira, Susana Cadinha, Ana Castro Neves, Patricia Barreira, Daniela Malheiro, J. P. Moreira Da Silva P134 Occupational airborne contact dermatitis to omeprazole Ružica Jurakic-Toncic, Suzana Ljubojevic, Petra Turcic P135 Ornidazole-induced fixed drug eruption confirmed by positive patch test on a residual pigmented lesion Liesbeth Gilissen, Sara Huygens, An Goossens P136 Repeated delayed reaction induced by amoxicillin and amoxicillin clavulanate Inmaculada Andreu, Ramon Lopez-Salgueiro, Alicia Martinez Romero, Pau Gomez Cabezas P137 Systemic photosensitivity from fenofibrate in a patient photo-sensitized to ketoprofen Liesbeth Gilissen, An Goossens Poster walk 16: HLA genetics (P138–P146) P138 A copy number variation in ALOX5 and PTGER1 is associated with nonsteroidal anti-inflammatory drugs induced urticaria and/or angioedema Pedro Ayuso Parejo, Maria Del Carmen Plaza-Serón, Inmaculada Doña, Natalia Blanca López, Carlos Flores, Luisa Galindo, Ana Molina, James Richard Perkins, Jose Antonio Cornejo-García, José Augusto García-Agúndez, Elena García-Martín, Paloma Campo, María Gabriela Canto, Miguel Blanca P139 Association of galectin-3 (LGALS3) single nucleotide polymorphisms with non-steroidal anti-inflammatory drugs-induced urticaria/angioedema José Antonio Cornejo-Garcia, Inmaculada Doña, Rosa María Guéant-Rodríguez, Natalia Blanca-López, María Carmen Plaza-Serón, Raquel Jurado-Escobar, Esther Barrionuevo, María Salas, María Luisa Galindo, Gabriela Canto, Miguel Blanca, Jean-Louis Guéant P140 Detection of T cell responses to ticlopidine using peripheral blood mononuclear cells from HLA-A*33:03+ healthy donors Toru Usui, Arun Tailor, Lee Faulkner, John Farrell, Ana Alfirevic, B. Kevin Park, Dean J. Naisbitt P141 Epistasis approaches to identify novel genes potentially involved in NSAIDs hypersensitivity James Richard Perkins, Jose Antonio Cornejo García, Oswaldo Trelles, Inmaculada Doña, Esther Barrionuevo, María Salas, María Auxiliadora Guerrero, Miguel Blanca, Alex Upton P142 Genetic predisposition of cold medicine related SJS/TEN with severe ocular complications Mayumi Ueta, Hiromi Sawai, Chie Sotozono, Katushi Tokunaga, Shigeru Kinoshita P143 HLA-B*13:01 and dapsone induced hypersensitivity in Thai population Chonlaphat Chonlaphat Sukasem, Patompong Satapornpong, Therdpong Tempark, Pawinee Rerknimitr, Kulprapat Pairayayutakul, Jettanong Klaewsongkram P144 HLA-B*15:02 alleles and lamotrigine-induced cutaneous adverse drug reactions in Thai Chonlaphat Sukasem, N. Koomdee, T. Jantararoungtong, S. Santon, A. Puangpetch, U. Intusoma, W. Tassaneeyakul, V. Theeramoke P145 HLA-B*38:01 and HLA-A*24:02 allele frequencies in Spanish patients with lamotrigine-induced SCARs Teresa Bellón, Elena Ramirez, Alberto Manuel Borobia, Hoi Tong, Jose Luis Castañer, Francisco José De Abajo P146 Overrepresentation of a class II HLA haplotype in severe hypersensitivity type I reactions to carboplatin Violeta Régnier Galvao, Rebecca Pavlos, Elizabeth Mckinnon, Kristina Williams, Alicia Beeghly-Fadiel, Alec Redwood, Elizabeth Phillips, Mariana Castells Poster walk 17: in vivo diagnosis + sIgE (P147–P154) P147 Absence of specific Ig-e against beta-lactams 9 months after an allergic reaction to amoxicillin-clavulanic acid Elisa Boni, Marina Russello, Marina Mauro P148 Drug provocation tests in suspected opioid allergy Kok Loong Ue, Krzysztof Rutkowski P149 Improvement to the specific IgE cut-off in the assess of β-lactamic allergy Victor Soriano Gomis, Jorge Frances Ferre, Angel Esteban Rodriguez, Vicente Cantó Reig, Javier Fernandez Sanchez P150 Initial false negative specific IgE to gelatin in a patient with gelatin-induced anaphylaxis Christine Breynaert, Erna Van Hoeyveld, Rik Schrijvers P151 Inmediate reactions to beta-lactam antibiotics: pattern of skin test response over the time Jose Julio Laguna Martinez, Rosario Gonzalez Mendiola, Javier Dionicio Elera, Cosmin Boteanu, Aranzazu Jimenez Blanco, Marta Del Pozo, Raquel Fuentes Irigoyen P152 New fluorescent dendrimeric antigens for the evaluation of dendritic cell maturation as a test to detect allergy reactions to amoxicillin Daniel Collado, Yolanda Vida, Francisco Najera, Ezequiel Perez-Inestrosa, Pablo Mesa-Antunez, Cristobalina Mayorga, María José Torres, Miguel Blanca P153 Positive skin test or positive specific IgE to penicillin does not predict penicillin allergy Line K. Tannert, Charlotte G. Mortz, Per Stahl Skov, Carsten Bindslev-Jensen P154 Significance of skin testing and in vitro-analysis of neuromuscular blocking agents in diagnosis of perioperative drug hypersensitivity: evaluation of a negative control population Wolfgang Pfützner, Hannah Dörnbach, Johanna Visse, Michele Rauber, Christian Möbs Poster walk 18: in vitro/ex vivo (P155–P158, P160–P164) P155 Diagnostic value of the lymphocyte toxicity assay (LTA) and the in vitro platelet toxicity assay (IPTA) for β-lactam allergy Abdelbaset A. Elzagallaai, Lindsey Chow, Awatif M. Abuzgaia, Michael J. Rieder P156 Enzyme linked immunospot assay used in the diagnosis of severe cutaneous adverse reactions to antimicrobials Alec Redwood, Jason Trubiano, Rebecca Pavlos, Emily Woolnough, Kaija Stautins, Christina Cheng, Elizabeth Phillips P157 Evaluation of in vitro diagnostic methods for identifying the culprit drugs in drug hypersensitivity Kenichi Kato, Hiroaki Azukizawa, Takaaki Hanafusa, Ichiro Katayama P158 Ex-vivo expanded skin-infiltrating T cells from severe drug eruptions are reactive with causative drugs: a possible novel method for determination of causative drugs Toshiharu Fujiyama, Hideo Hashizume, Takatsune Umayahara, Taisuke Ito, Yoshiki Tokura P160 In vitro release of IL-2, IL-5 and IL-13 in diagnosis of patients with delayed-type nickel hypersensitivity Mira Silar, Mihaela Zidarn, Helena Rupnik, Peter Korosec P161 Single cell analysis of drug responsive T cells; identification of candidate drug reactive T cell receptors in abacavir and carbamazepine hypersensitivity Alec James Redwood, Kaija Strautins, Katie White, Abha Chopra, Katherine Konvinse, Shay Leary, Rebecca Pavlos, Simon Mallal, Elizabeth Phillips P162 Specificity and sensitivity of LTT in DRESS: analysis of agreement with the Spanish pharmacovigilance system probability algorithm Rosario Cabañas, Elena Ramirez, Ana María Fiandor, Teresa Bellón P163 The role of interleukin-22 in β-lactam hypersensitivity Andrew Sullivan, Paul Whitaker, Daniel Peckham, B. Kevin Park, Dean J. Naisbitt P164 Vancomycin-specific T cell responses and teicoplanin cross-reactivity Wei Yann Haw, Marta E. Polak, Carolann Mcguire, Michael R. Ardern-Jones Poster walk 19: BAT and biomarkers (P165–P173) P165 A combination of early biomarkers useful for the prediction of severe ADRs Yumi Aoyama, Tetsuo Shiohara P166 Basophil activation test in the diagnostic approach of reactions during general anaesthesia Ana Moreira, Susana Cadinha, Patrícia Barreira, Ana Castro Neves, Daniela Malheiro, Sara Correia, J. P. Moreira Da Silva P167 IL-10 can be related to successful desensitization Asli Gelincik, Semra Demir, Fatma Sen, Hamza Ugur Bozbey, Muge Olgac, Derya Unal, Raif Coskun, Bahauddin Colakoglu, Suna Buyuozturk, Esin Çatin-Aktas, Gunnur Deniz P168 Immediate reactions to proton pump inhibitors: value of basophil activation test Maria Salas, Jose Julio Laguna, Esther Barrionuevo, J. Dionicio, Tahia Fernandez, R. Gonzalez-Mendiola, I. Olazabal, Maria Dolores Ruiz, Miguel Blanca, Cristobalina Mayorga, Maria José Torres P169 Improvement of the elevated tryptase criterion to discriminate IgE from non-IgE mediated allergic reactions Gabriel Gastaminza, Alberto Lafuente, Carmen D’Amelio, Amalia Bernad, Olga Vega, Roselle Catherine Madamba, M. Jose Goikoetxea, Marta Ferrer, Jorge Núñez P170 Low expression of Tim-3 could serve as a biomarker for control and diagnose maculopapular exanthema induced by drugs Tahia Diana Fernández, Inmaculada Doña, Francisca Palomares, Rubén Fernández, Maria Salas, Esther Barrionuevo, Maria Isabel Sanchez, Miguel Blanca, Maria José Torres, Cristobalina Mayorga P171 Role of basophil activation test using two different activation markers for the diagnosis of allergy to fluoroquinolones Esther Barrionuevo, Tahía Fernandez, Arturo Ruiz, Adriana Ariza, Maria Salas, Inmaculada Doña, Ana Molina, Miguel Blanca, Maria Jose Torres, Cristobalina Mayorga P172 The importance of basophil activation test in anaphylaxis due to celecoxib Amalia Bernad Alonso, Carmen D’Amelio Garófalo, Olga Vega Matute, Marta Ferrer Puga, María José Goikoetxea Lapresa, Roselle Catherine Yu Madamba, Gabriel Gastaminza Lasarte P173 The role of basophil activation test in the diagnosis of immediate type drug hypersensitivity to betalactam antibiotics Antonia Thinnes, Hans F. Merk, Jens Malte Baron, Martin Leverkus, Galina Balakirski Poster walk 20: TCR recognition, cellular (P174–P183) P174 Characterisation of the effect of co-inhibitory signalling on the activation of drug-derived antigen-specific T-cells Andrew Gibson, Monday Ogese, Lee Faulkner, B. Kevin Park, Dean J. Naisbitt P175 Characterization of drug hapten-specific T cell responses in piperacillin hypersensitive patients Zaid Al-Attar, Fiazia Yaseen, Xiaoli Meng, Rozalind Jenkins, Paul Whitaker, Daniel Peckham, Lee Faulkner, John Farrel, Kevin Park, Dean Naisbitt P176 Characterization of the response of T-cells to telaprevir and its metabolite in normal volunteers Zaid Al-Attar, Khetam Alhilali, Yanni Xue, John Farrell, Lee Faulkner, Kevin Park, Dean Naisbitt P177 Characterization of the T cell receptor signatures of drug-responsive T cells Patricia Illing, Nicole Mifsud, Heidi Fettke, Jeffrey Lai, Rebecca Ho, Patrick Kwan, Anthony Purcell P178 Defining the signals between hepatocytes and immune cells in idiosyncratic drug-induced liver injury (DILI) Monday O. Ogese, Lee Faulkner, B. Kevin Park, Catherine Betts, Dean J. Naisbitt P179 Development of novel chemicals that do not bind to HLA-B*57:01 or activate CD8 + T-cells through modification of the 6-amino cyclopropyl group of abacavir Paul Thomson, John Farrell, Mohammad Alhaidari, Neill Berry, Paul M. O’Neill, B. Kevin Park, Dean J. Naisbitt P180 Generation and characterization of dapsone- and nitroso-dapsone-specific T-cell clones using lymphocytes from healthy volunteers Abdulaziz Alzahrani, Monday O. Ogese, John Farrell, Lee Faulkner, Andrew Gibson, Arun Tailor, B. Kevin Park, Dean J. Naisbitt P181 Identification of benzylpenicillin-hapten peptides responsible for naïve T-cell activation and immunization of allergic patients to penicillin Marie Eliane Azoury, Lucia Fili, Rami Bechara, Noémie Scornet, Cathy Nhim, Richard Weaver, Nancy Claude, Delphine Joseph, Bernard Maillere, Paola Parronchi, Marc Pallardy P182 Massive expansion of clonotypic and polycytotoxic CD8+ T cells in toxic epidermal necrolysis Axel Patrice Villani, Aurore Rozières, Benoît Bensaïd, Mathilde Tardieu, Floriane Albert, Virginie Mutez, Tugba Baysal, Marc Pallardy, Janet Maryanski, Jean-François Nicolas, Osami Kanagawa, Marc Vocanson P183 Pharmaco-immunological synapse of HLA-drug-TCR in SCAR Shuen-Iu Hung Poster walk 21: new in vitro methods, haptens, etc. (P184–P194) P184 Amoxicillin-clavulanate forms distinct multiple haptenic structures on human serum albumin in patients Xiaoli Meng, Arun Tailor, Caroline J. Harrison, Rosalind E. Jenkins, Paul Whitaker, Neil S. French, Dean J. Naisbitt, B. Kevin Park P185 Dendrimeric antigens for studying the influence of penicillin determinants orientation on IgE recognition Maria Isabel Montañez, Cristobalina Mayorga, Francisco Najera, Adriana Ariza, Tahia D. Fernandez, Maria Salas, Angela Martin-Serrano, Miguel Blanca, Ezequiel Perez-Inestrosa, Maria Jose Torres P186 Dendrimeric antigens on solid supports: designed materials for IgE quantification Yolanda Vida, Maria Isabel Montañez, Noemi Molina, Daniel Collado, Francisco Najera, Adriana Ariza, Maria Jose Torres, Cristobalina Mayorga, Ezequiel Perez-Inestrosa P187 Development of a screening assay for drug hypersensitivity using naïve T cells from donors with seven different HLA class I risk alleles Lee Faulkner, Sally Wood, Ana Alfirevic, Munir Pirmohamed, Dean J. Naisbitt, B. Kevin Park P188 Different patterns of recognition of structures derived from amoxicillin by IgE antibodies from patients with immediate hypersensitivity reactions to betalactams Adriana Ariza, Cristobalina Mayorga, María Isabel Montañez, María Salas, Inmaculada Doña, Ángela Martín-Serrano, Ezequiel Pérez-Inestrosa, Dolores Pérez-Sala, Miguel Blanca, Antonio E. Guzmán, María José Torres P189 High-resolution typing of HLA polymorphism and T-cell receptor repertoire for severe adverse drug reactions based on the cost-effective next-generation sequencing approaches Tai-Ming Ko, Yuan-Tsong Chen, Jer-Yuarn Wu P190 Identification and fate of intracellular proteins haptenated by amoxicillin Francisco J. Sánchez-Gómez, Juan M. González-Morena, Yolanda Vida, Ezequiel Pérez-Inestrosa, Miguel Blanca, María J. Torres, Dolores Pérez-Sala P191 In vitro detection of terbinafine protein adducts Arun Tailor, Toru Usui, Yanni Xue, Xiaoli Meng, Dean J. Naisbitt, B. Kevin Park P192 MicroRNAs dysregulation in PBMCs from drug hypersensitivity patients during drug challenge in vitro Alejandra Monroy Arreola, Jesus Agustin Badillo Corona, Silvia Mendez Flores, Judith Dominguez Cherit, Dean J. Naisbitt, Noe Valentin Duran Figueroa, Jose Luis Castrejon Flores P193 NSAIDs-exacerbated cutaneous disease: high throughput gene expression profiling José Antonio Cornejo-García, James Perkins, Natalia Blanca-López, Diana Pérez-Alzate, Raquel Jurado-Escobar, Inmaculada Doña, Gador Bogas, María J. Torres, Gabriela Canto, Miguel Blanca P194 Utility of skin tests in non-immediate reactions to amoxicillin Luis Mario Tubella Marti, Fernando Pineda De La Losa, Francisca Arribas Poves, Jaime Tubella Lopez, Teodora Lopez Santiag

Poor timing and failure of source control are risk factors for mortality in critically ill patients with secondary peritonitis

Purpose: To describe data on epidemiology, microbiology, clinical characteristics and outcome of adult patients admitted in the intensive care unit (ICU) with secondary peritonitis, with special emphasis on antimicrobial therapy and source control. Methods: Post hoc analysis of a multicenter observational study (Abdominal Sepsis Study, AbSeS) including 2621 adult ICU patients with intra-abdominal infection in 306 ICUs from 42 countries. Time-till-source control intervention was calculated as from time of diagnosis and classified into 'emergency' (< 2 h), 'urgent' (2-6 h), and 'delayed' (> 6 h). Relationships were assessed by logistic regression analysis and reported as odds ratios (OR) and 95% confidence interval (CI). Results: The cohort included 1077 cases of microbiologically confirmed secondary peritonitis. Mortality was 29.7%. The rate of appropriate empiric therapy showed no difference between survivors and non-survivors (66.4% vs. 61.3%, p = 0.1). A stepwise increase in mortality was observed with increasing Sequential Organ Failure Assessment (SOFA) scores (19.6% for a value ≤ 4-55.4% for a value > 12, p < 0.001). The highest odds of death were associated with septic shock (OR 3.08 [1.42-7.00]), late-onset hospital-acquired peritonitis (OR 1.71 [1.16-2.52]) and failed source control evidenced by persistent inflammation at day 7 (OR 5.71 [3.99-8.18]). Compared with 'emergency' source control intervention (< 2 h of diagnosis), 'urgent' source control was the only modifiable covariate associated with lower odds of mortality (OR 0.50 [0.34-0.73]). Conclusion: 'Urgent' and successful source control was associated with improved odds of survival. Appropriateness of empirical antimicrobial treatment did not significantly affect survival suggesting that source control is more determinative for outcome

Antimicrobial Lessons From a Large Observational Cohort on Intra-abdominal Infections in Intensive Care Units

evere intra-abdominal infection commonly requires intensive care. Mortality is high and is mainly determined by disease-specific characteristics, i.e. setting of infection onset, anatomical barrier disruption, and severity of disease expression. Recent observations revealed that antimicrobial resistance appears equally common in community-acquired and late-onset hospital-acquired infection. This challenges basic principles in anti-infective therapy guidelines, including the paradigm that pathogens involved in community-acquired infection are covered by standard empiric antimicrobial regimens, and second, the concept of nosocomial acquisition as the main driver for resistance involvement. In this study, we report on resistance profiles of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis and Enterococcus faecium in distinct European geographic regions based on an observational cohort study on intra-abdominal infections in intensive care unit (ICU) patients. Resistance against aminopenicillins, fluoroquinolones, and third-generation cephalosporins in E. coli, K. pneumoniae and P. aeruginosa is problematic, as is carbapenem-resistance in the latter pathogen. For E. coli and K. pneumoniae, resistance is mainly an issue in Central Europe, Eastern and South-East Europe, and Southern Europe, while resistance in P. aeruginosa is additionally problematic in Western Europe. Vancomycin-resistance in E. faecalis is of lesser concern but requires vigilance in E. faecium in Central and Eastern and South-East Europe. In the subcohort of patients with secondary peritonitis presenting with either sepsis or septic shock, the appropriateness of empiric antimicrobial therapy was not associated with mortality. In contrast, failure of source control was strongly associated with mortality. The relevance of these new insights for future recommendations regarding empiric antimicrobial therapy in intra-abdominal infections is discussed.Severe intra-abdominal infection commonly requires intensive care. Mortality is high and is mainly determined by diseasespecific characteristics, i.e. setting of infection onset, anatomical barrier disruption, and severity of disease expression. Recent observations revealed that antimicrobial resistance appears equally common in community-acquired and late-onset hospital-acquired infection. This challenges basic principles in anti-infective therapy guidelines, including the paradigm that pathogens involved in community-acquired infection are covered by standard empiric antimicrobial regimens, and second, the concept of nosocomial acquisition as the main driver for resistance involvement. In this study, we report on resistance profiles of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis and Enterococcus faecium in distinct European geographic regions based on an observational cohort study on intra-abdominal infections in intensive care unit (ICU) patients. Resistance against aminopenicillins, fluoroquinolones, and third-generation cephalosporins in E. coli, K. pneumoniae and P. aeruginosa is problematic, as is carbapenem-resistance in the latter pathogen. For E. coli and K. pneumoniae, resistance is mainly an issue in Central Europe, Eastern and South-East Europe, and Southern Europe, while resistance in P. aeruginosa is additionally problematic in Western Europe. Vancomycin-resistance in E. faecalis is of lesser concern but requires vigilance in E. faecium in Central and Eastern and South-East Europe. In the subcohort of patients with secondary peritonitis presenting with either sepsis or septic shock, the appropriateness of empiric antimicrobial therapy was not associated with mortality. In contrast, failure of source control was strongly associated with mortality. The relevance of these new insights for future recommendations regarding empiric antimicrobial therapy in intra-abdominal infections is discussed