Then You Fall Off : Youth Experiences and Responses to Transitioning to Homelessness

Introduction: This study aims to broaden our understanding of the experience of homelessness and unstable housing in youth. While quantitative research highlights risk factors associated with experiencing homelessness as a youth, little qualitative research has been conducted to explore the lived experience of this transition to homelessness or unstable housing and how youth respond to becoming homeless. This study utilizes data from youth descriptions of their experiences to understand the context of the transition to homelessness and how youth manage this transition. Methods: A qualitative study with a quantitative component was conducted with a nonprobability sample of homeless youth aged 14-24 recruited from shelters, drop-in centers, and magnet events in a large urban area in the Southwest. Four qualitative researchers used content analysis to assess themes that emerged related to transitions to homelessness. Results: A predominately minority (88%) sample of sheltered (67%) and unsheltered (33%) youth (n=64) described their experience of and responses to transitioning to homelessness. Three main themes emerged relating to transitioning to homelessness; family homelessness, histories of foster care, and non-supportive family processes. Youth described how these experiences manifested and influenced their transition into homelessness. In response to homelessness three dominant themes emerged; self-reliance, hope, and resilience. Discussion: The data highlight the unique issues of homeless youth and how they respond to circumstantial challenges. While homeless youth experience lifetime adversities that lead to homelessness, they respond to these circumstantial challenges with self-reliance, hope, and maintaining resilience. Interventions aiming to facilitate health behaviors and improve self-sufficiency in homeless youth should tap into these positive responses to improve self-care strategies, service utilization, and help homeless youth reduce risk behaviors

SISTEMA DE ABASTECIMIENTO Y LA GESTIÓN ADMINISTRATIVA DE LA UNIDAD DE LOGISTICA EN LA UNIDAD EJECUTORA 403 RED DE SALUD LEONCIO PRADO, 2019

La tesis asumió como objetivo principal analizar de qué manera el sistema de abastecimiento se relaciona con la gestión administrativa en la Unidad de Logística de la Unidad Ejecutora 403 Red de Salud Leoncio Prado, 2019. El propósito fue desarrollado a un nivel descriptivo – correlacional, con diseño transversal de tipo no experimental, metodológicamente se obtuvo mediante una encuesta, empleando un instrumento; uno para la variable independiente: sistema de abastecimiento, que consta de siete preguntas respecto a; programación multianual de bienes y servicios, gestión de adquisiciones y administración de bienes; segundo para la variable dependiente: gestión administrativa, que consta de siete preguntas respecto a; planificación, organización y control. La muestra fue de 26 servidores públicos. Para el análisis de datos se utilizó estadísticas descriptivas no paramétricas y pruebas normalidad de rho de Spearman para demostrar las hipótesis. Los resultados de análisis de correlación de rho Spearman se obtuvo un valor 0,815 y la significancia de p ≤ 0.0001, demostrando relación entre el sistema de abastecimiento y la gestión administrativa Las importantes dimensiones del sistema de abastecimiento que se relacionan significativamente con la gestión administrativa en la Unidad de Logística de la Unidad Ejecutora 403 Red de Salud Leoncio Prado, son principalmente la programación multianual de bienes y servicios, gestión de adquisiciones y administración de bienes.Tesi

Avaliação da qualidade microbiológica de queijos Minas Frescal comercializados em supermercados da região Extremo-Oeste de Santa Catarina, Brasil

O queijo Minas Frescal possui características físico-químicas adequadas para o crescimento de microrganismos e tem sido frequentemente associado a várias doenças transmitidas por alimentos (DTAs). Assim, o objetivo deste trabalho foi avaliar a qualidade microbiológica de queijos Minas Frescal comercializados em supermercados da região Extremo-Oeste catarinense, por meio da contagem de Staphylococcus coagulase positiva, coliformes termotolerantes e da presença de Salmonella sp. e Listeria monocytogenes. Foram coletadas 30 amostras de queijo Minas Frescal comercializadas em supermercados das cidades de São Miguel do Oeste, Maravilha, Pinhalzinho e Modelo, SC. As análises microbiológicas foram realizadas de acordo com a recomendação e exigências da RDC n. 12 de 2 de janeiro de 2001 (Anvisa) e a metodologia utilizada para efetuar as análises microbiológicas foi baseada na Instrução Normativa n. 62 de 26 de agosto de 2003 do Ministério da Agricultura, Pecuária e Abastecimento (MAPA). Os resultados revelaram que 7% das amostras estavam fora dos padrões estabelecidos pela legislação, apresentando contaminação por coliformes termotolerantes. Não houve contaminação por L. monocytogenes, Salmonella sp. e Staphylococcus coagulase positiva. Embora o número de amostras contaminadas tenha sido baixo, indica que houve falhas nas práticas de fabricação desses alimentos, o que demonstra a importância de manter o rigor no sistema de controle de qualidade nos laticínios. Palavras-chave:  Contaminação microbiológica. Doenças transmitidas por alimentos. Controle de qualidade. Segurança dos alimentos

Effects of training multiple form classes on acquisition, generalization and maintenance of word retrieval in a single subject

A study by McNeil et nl. reported no generalization effects in two individuals with aphasia following application of a word finding treatment in which subjects were trained sequentially on lexical items arranged by form class. The present investigation examined, in one of the two subjects from the original study, whether training on lexical items from a variety of form classes concurrently would result in greater response generalization than was observed previously in this subject. Results replicated earlier findings with positive acquisition and maintenance effects and little evidence of generalization to untrained items within or across form classes

DNA Double-Strand Break Repair Genes and Oxidative Damage in Brain Metastasis of Breast Cancer

Background Breast cancer frequently metastasizes to the brain, colonizing a neuro-inflammatory microenvironment. The molecular pathways facilitating this colonization remain poorly understood. Methods Expression profiling of 23 matched sets of human resected brain metastases and primary breast tumors by two-sided paired t test was performed to identify brain metastasis–specific genes. The implicated DNA repair genes BARD1 and RAD51 were modulated in human (MDA-MB-231-BR) and murine (4T1-BR) brain-tropic breast cancer cell lines by lentiviral transduction of cDNA or short hairpin RNA (shRNA) coding sequences. Their functional contribution to brain metastasis development was evaluated in mouse xenograft models (n = 10 mice per group). Results Human brain metastases overexpressed BARD1 and RAD51 compared with either matched primary tumors (1.74-fold, P < .001; 1.46-fold, P < .001, respectively) or unlinked systemic metastases (1.49-fold, P = .01; 1.44-fold, P = .008, respectively). Overexpression of either gene in MDA-MB-231-BR cells increased brain metastases by threefold to fourfold after intracardiac injections, but not lung metastases upon tail-vein injections. In 4T1-BR cells, shRNA-mediated RAD51 knockdown reduced brain metastases by 2.5-fold without affecting lung metastasis development. In vitro, BARD1- and RAD51-overexpressing cells showed reduced genomic instability but only exhibited growth and colonization phenotypes upon DNA damage induction. Reactive oxygen species were present in tumor cells and elevated in the metastatic neuro-inflammatory microenvironment and could provide an endogenous source of genotoxic stress. Tempol, a brain-permeable oxygen radical scavenger suppressed brain metastasis promotion induced by BARD1 and RAD51 overexpression. Conclusions BARD1 and RAD51 are frequently overexpressed in brain metastases from breast cancer and may constitute a mechanism to overcome reactive oxygen species–mediated genotoxic stress in the metastatic brain

Interdisciplinarity in the methodology of teaching in Health: A Literature Review.

The objective of this article is to carry out a sweep of the current medical literature on the relationship between interdisciplinarity in the methodology of teaching in Health. keywords “Teaching, Health, Interdisciplinarity, Health Education”. Articles with more than 20 years of publication or that did not fit within the scope of the research were excluded. In all, 10 articles that fit within the search patterns were selected. It is concluded that most publications promote the need for changes in Health Education in higher education and in learning, knowledge and management of the SUS, in order to promote the Significant Learning of graduates in their professional careers.The objective of this article is to carry out a sweep of the current medical literature on the relationship between interdisciplinarity in the methodology of teaching in Health. keywords “Teaching, Health, Interdisciplinarity, Health Education”. Articles with more than 20 years of publication or that did not fit within the scope of the research were excluded. In all, 10 articles that fit within the search patterns were selected. It is concluded that most publications promote the need for changes in Health Education in higher education and in learning, knowledge and management of the SUS, in order to promote the Significant Learning of graduates in their professional careers

Relato de experiência em Educação em Saúde para gestantes na pandemia da COVID-19: utilização de tecnologias virtuais para a promoção da autonomia e prevenção de agravos

Introduction: Health education for pregnant women is one of the main ways to guarantee comprehensive and person-centered care. However, in the midst of a viral pandemic scenario, face-to-face and collective spaces aimed at exchanging experiences and linking information between pregnant women and health professionals in the primary health care network were suspended for being considered a health risk. The objective was to report the experiences lived through socio-educational work in order to encourage self-care and promote access to information about the reproductive and sexual rights of women during pregnancy. Report: The activity was divided into two periods for the organization and application of the activity, the first to select themes, train members, produce material, establish partnerships, enable means of dissemination with Community Health Agents, present the proposal to pregnant women for the adhesion to the project and creation of the group with a previous presentation interaction. The second period was contemplated for the execution of the programmed actions through videos and teaching materials via the WhatsApp platform, in addition to the creation of a space for women to clear up their doubts and express their anxieties. Finally, as a form of impact assessment, a personalized and thematic questionnaire for pregnant women was applied, with the inclusion of qualitative aspects of the absorbed knowledge and evaluations of the lived experience. Discussion: Factors related to the importance of health education, access to health services, the context of COVID-19 and pregnancy and the preservation of rights, as promoters of health and autonomy. Conclusion: The project proved to be effective in strengthening a support network in the midst of the pandemic, with the creation of links between the participants and coordinators, which fostered the initiative of sharing knowledge in health promotion and prevention in the period. pregnancy.Introdução: A educação em saúde para gestantes é uma das principais formas da garantia de um atendimento integral e centrado na pessoa. Entretanto, em meio a um cenário de pandemia viral, os espaços presenciais e coletivos voltados para troca de experiências e vinculação de informações entre gestantes e profissionais da saúde na rede primária de saúde foram suspensos por serem considerados um risco sanitário. Objetivou-se relatar as experiências vividas através de um trabalho socioeducativo a fim de estimular o autocuidado e promover acesso a informações sobre os direitos reprodutivos e sexuais das mulheres em meio a gestação. Relato: A atividade foi dividida em dois períodos para a organização e aplicação da atividade, o primeiro para selecionar temáticas, capacitar integrantes, produção de material, estabelecer parcerias, viabilizar meios de divulgação com Agentes Comunitários de Saúde, apresentar a proposta às gestantes para a adesão ao projeto e criação do grupo com uma interação prévia de apresentação. Já o segundo período foi contemplado para a execução das ações programadas por meio de vídeos e materiais didáticos via aplicativo de mensagens, além da criação de um espaço para as mulheres tirarem suas dúvidas e expressarem suas angústias. Por fim, como forma de avaliação de impacto aplicou-se um questionário personalizado e temático para as gestantes, com a inclusão de aspectos qualitativos dos conhecimentos absorvidos e avaliativos da experiência vivenciada. Discussão: Fatores relacionados a importância da educação em saúde, acesso ao serviço de saúde, contexto do COVID-19 e gestação e a preservação de direitos, enquanto promotores de saúde e autonomia. Conclusão: O projeto mostrou-se efetivo no âmbito do fortalecimento de uma rede de apoio em meio a pandemia, com a criação de vínculos entre as participantes e coordenadores, o que fomentou a iniciativa do compartilhamento de conhecimentos de promoção e prevenção em saúde no período gravídico

Feeder layer- and animal product-free culture of neonatal foreskin keratinocytes: improved performance, usability, quality and safety

Since 1987, keratinocytes have been cultured at the Queen Astrid Military Hospital. These keratinocytes have been used routinely as auto and allografts on more than 1,000 patients, primarily to accelerate the healing of burns and chronic wounds. Initially the method of Rheinwald and Green was used to prepare cultured epithelial autografts, starting from skin samples from burn patients and using animal-derived feeder layers and media containing animal-derived products. More recently we systematically optimised our production system to accommodate scientific advances and legal changes. An important step was the removal of the mouse fibroblast feeder layer from the cell culture system. Thereafter we introduced neonatal foreskin keratinocytes (NFK) as source of cultured epithelial allografts, which significantly increased the consistency and the reliability of our cell production. NFK master and working cell banks were established, which were extensively screened and characterised. An ISO 9001 certified Quality Management System (QMS) governs all aspects of testing, validation and traceability. Finally, as far as possible, animal components were systematically removed from the cell culture environment. Today, quality controlled allograft production batches are routine and, due to efficient cryopreservation, stocks are created for off-the-shelf use. These optimisations have significantly increased the performance, usability, quality and safety of our allografts. This paper describes, in detail, our current cryopreserved allograft production process

Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome.

The evolution of land flora transformed the terrestrial environment. Land plants evolved from an ancestral charophycean alga from which they inherited developmental, biochemical, and cell biological attributes. Additional biochemical and physiological adaptations to land, and a life cycle with an alternation between multicellular haploid and diploid generations that facilitated efficient dispersal of desiccation tolerant spores, evolved in the ancestral land plant. We analyzed the genome of the liverwort Marchantia polymorpha, a member of a basal land plant lineage. Relative to charophycean algae, land plant genomes are characterized by genes encoding novel biochemical pathways, new phytohormone signaling pathways (notably auxin), expanded repertoires of signaling pathways, and increased diversity in some transcription factor families. Compared with other sequenced land plants, M. polymorpha exhibits low genetic redundancy in most regulatory pathways, with this portion of its genome resembling that predicted for the ancestral land plant. PAPERCLIP