Looking at cell mechanics with atomic force microscopy: Experiment and theory

This review reports on the use of the atomic force microscopy in the investigation of the mechanical properties of cells. It is shown that the technique is able to deliver information about the cell surface properties (e.g., topography), the Young modulus, the viscosity, and the cell the relaxation times. Another aspect that this short review points out is the utilization of the atomic force microscope to investigate basic questions related to materials physics, biology, and medicine. The review is written in a chronological way to offer an overview of phenomenological facts and quantitative results to the reader. The final section discusses in detail the advantages and disadvantages of the Hertz and JKR models. A new implementation of the JKR model derived by Dufresne is presented

Formyl peptide chemotaxis receptors on the rat neutrophil: Experimental evidence for negative cooperativity

To examine the existence of negative cooperativity among formyl peptide chemotaxis receptors, steady-state binding of f Met-Leu-[ 3 H]Phe to viable rat neutrophils and their purified plasma membranes was measured and the data were subjected to statistical analysis and to computer curve fitting using the NONLIN computer program. Curvilinear, concave upward Scatchard plots were obtained. NONLIN and statistical analyses of the binding data indicated that a two-saturable-sites model was preferable to a one-saturable-site model and statistically valid by the F-test (P < 0.1). In addition, Hill coefficients of 0.80 ± 0.02 were obtained. Kinetic dissociation experiments using purified plasma membranes showed evidence of site-site interactions of the destabilizing type (negative cooperativity). Thus, unlabeled f Met-Leu-Phe accelerated the dissociation of f Met-Leu-[ 3 H]Phe under conditions where no rebinding of radioligand occurred. The rate of dissociation of f Met-Leu-[ 3 H]Phe from the plasma membranes was dependent on the fold excess of unlabeled f Met-Leu-Phe used in the dilution medium; at the highest concentration tested (10,000-fold excess), the dissociation rate was more than double the dissociation rate seen with dilution alone, In addition, occupancy-dependent affinity was ascertained directly by studying the effect of increasing fractional receptor saturation with labeled ligand on the dissociation rate of the receptor-bound labeled ligand. These data showed that the f Met-Leu-[ 3 H]Phe dissociation rate was dependent on the degree of binding site occupancy over the entire biologically relevant range of formyl peptide concentrations. Furthermore, monitoring of the time course of dissociation of the receptor/f Met-Leu-[ 3 H]Phe complex as a function of receptor occupancy revealed that receptor affinity for f Met-Leu-Phe remained occupancy-dependent during the entire time of dissociation examined (up to 10 min). Finally, the average affinity profile of the equilibrium binding data demonstrated a 60% decrease in receptor affinity in changing from the high affinity to the low affinity conformation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/38446/1/240270406_ftp.pd

The Jewish Merchant-Diplomat Isaac Surgun and the Dutch-Mysore Conflict, 1765-1791

Fischel Walter Joseph. The Jewish Merchant-Diplomat Isaac Surgun and the Dutch-Mysore Conflict, 1765-1791. In: Revue des études juives, tome 126, n°1, janvier-mars 1967. pp. 27-53

The Activities of a Jewish Merchant House in Bengal (1786-1798). A Contribution to the Economic History of London Jews in India

Fischel Walter Joseph. The Activities of a Jewish Merchant House in Bengal (1786-1798). A Contribution to the Economic History of London Jews in India. In: Revue des études juives, tome 123, n°3-4, juillet-décembre 1964. pp. 433-498