Projeto Escola de Tempo Integral como pol?tica p?blica em escolas de educa??o b?sica de Diamantina: mais tempo de uma outra educa??o?

Esta pesquisa tem como objetivo principal investigar a implanta??o do Projeto Escola de Tempo Integral (PROETI) como pol?tica p?blica em escolas de educa??o b?sica em Diamantina-MG, a sua trajet?ria, bem como analisar se o ?mais tempo? nestas escolas ressignifica mais tempo de uma outra educa??o, na percep??o dos profissionais envolvidos no Projeto. Este estudo teve como recorte temporal o per?odo de 2008 a 2016. Para conduzir esta investiga??o, a metodologia utilizada envolveu a pesquisa qualitativa, tendo como procedimentos o estudo de caso, a pesquisa bibliogr?fica e a documental, al?m do instrumento metodol?gico da entrevista semiestruturada com os envolvidos no Projeto. O aporte te?rico desta pesquisa foi norteado por autores que abordam a tem?tica da educa??o integral/tempo integral como pol?tica p?blica, dentre eles: ARROYO (1998, 2000, 2006, 2012), CAVALIERE (2002, 2007), GUAR? (2006), MOLL (2004, 2009, 2012), MORAES (2013) e TEIXEIRA (1969, 1976, 1999). A discuss?o e a interpreta??o dos dados coletados foram realizadas mediante a an?lise de conte?do na perspectiva de Bardin (2011). Como resultado, este estudo concluiu que os projetos e programas relacionados ? educa??o integral nas escolas pesquisadas est?o em processo de implementa??o, mas ainda n?o est?o consolidados conforme as diretrizes estabelecidas pela SEE/MG. O tempo a mais nestas escolas, do PROETI ao PEI, ainda n?o significa mais tempo de uma outra educa??o, mas uma importante experi?ncia da amplia??o da jornada de perman?ncia di?ria dos alunos, tendo em vista o car?ter assistencialista desempenhado por estes projetos. Estima-se que o resultado desta pesquisa possa contribuir para reflex?es acerca da implanta??o e funcionamento dos projetos e programas educacionais com amplia??o de jornada escolar di?ria, servindo de subs?dios para implanta??o em outras escolas, e principalmente, direcionando um olhar mais atento para as dificuldades apontadas, bem como para as possibilidades de desenvolvimento de atividades que realmente sejam traduzidas em mais educa??o, entendida como educa??o integral.Disserta??o (Mestrado Profissional) ? Programa de P?s-Gradua??o em Educa??o, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017.This research has as main objective to investigate the implantation of the School of Integral Time Project (PROETI) as a public policy in primary schools in Diamantina-MG, its trajectory, as well as to analyze if the "more time" in these schools re-signifies more time of another education, in the perception of the professionals involved in the Project. This study had as a temporary clipping the period from 2008 to 2016. In order to conduct this research, the methodology used involved qualitative research, having as procedures the case study, bibliographical and documentary research, besides the methodological instrument of the semi-structured interview with those involved in the Project. The theoretical contribution of this research was guided by authors who approach the theme of integral education / full time as public policy, among them: ARROYO (1998, 2000, 2006, 2012), CAVALIERE (2002, 2007), GUAR? (2006), MOLL (2004, 2009, 2012), MORAES (2013) and TEIXEIRA (1969, 1976, 1999). The discussion, as well as the interpretation of the data collected, were carried out through content analysis from the perspective of Bardin (2011). As a result, this study concluded that the projects and programs related to integral education in the schools surveyed are in the process of being implemented, but are not yet consolidated according to the guidelines established by the SEE / MG. The longer time in these schools, from PROETI to PEI, still does not mean more time for another education, but an important experience of extending the students' daily stay, in view of the assistentialist character played by these projects. It is estimated that the results of this research may contribute to reflections on the implementation and functioning of educational projects and programs with an increase in the daily school day, serving as subsidies for implantation in other schools, and especially, directing a closer look at the difficulties pointed out, as also for the possibilities of developing activities that really translate into more education, understood as integral education

MicroRNA-153 targeting of KCNQ4 contributes to vascular dysfunction in hypertension.

AIMS: Kv7.4, a voltage-dependent potassium channel expressed throughout the vasculature, controls arterial contraction and is compromised in hypertension by an unknown mechanism. MicroRNAs (miRs) are post-transcriptional regulators of protein production and are altered in disease states such as hypertension. We investigated whether miRs regulate Kv7.4 expression. METHODS AND RESULTS: In renal and mesenteric arteries (MAs) of the spontaneously hypertensive rat (SHR), Kv7.4 protein decreased compared with the normotensive (NT) rat without a decrease in KCNQ4 mRNA, inferring that Kv7.4 abundance was determined by post-transcriptional regulation. In silico analysis of the 3' UTR of KCNQ4 revealed seed sequences for miR26a, miR133a, miR200b, miR153, miR214, miR218, and let-7d with quantitative polymerase chain reaction showing miR153 increased in those arteries from SHRs that exhibited decreased Kv7.4 levels. Luciferase reporter assays indicated a direct targeting effect of miR153 on the 3' UTR of KCNQ4. Introduction of high levels of miR153 to MAs increased vascular wall thickening and reduced Kv7.4 expression/Kv7 channel function compared with vessels receiving a non-targeting miR, providing a proof of concept of Kv7.4 regulation by miR153. CONCLUSION: This study is the first to define a role for aberrant miR153 contributing to the hypertensive state through targeting of KCNQ4 in an animal model of hypertension, raising the possibility of the use of miR153-related therapies in vascular disease

Array-comparative genomic hybridization as a novel high-throughput approach in bladder cancer treatment

info:eu-repo/semantics/publishedVersio

Associação entre consumo de álcool e alimentação com baixo valor nutritivo entre adolescentes escolares

Objective: to verify the association between alcohol consumption with a sociodemographic profile and inadequate diet among school adolescents. Materials and methods: epidemiological study with cross-sectional design, conducted with high school students. The sample was of the probabilistic type by clusters. For data collection, a self-administered questionnaire was used. The dependent variable was alcohol consumption. Binary Logistic Regression was conducted, adopting a significance level of 5% and the analyzes were corrected by the design effect. Results: 2.040 adolescents in 21 schools participated in the study, 54.4% (1103) of whom were female, with almost 60% (1198) of adolescents aged 16 to 17 years. The use of alcohol by adolescents was related to 39.1% (774), with this consumption being higher among boys (40.3%) when compared to girls (36.2%). There was a higher probability of alcohol consumption among adolescents who had higher education (OR=1.358) and who consumed soda frequently (OR=1,580). Conclusion: in this study, alcohol consumption was associated with the sociodemographic profile and inadequate diet of school adolescents.Objetivo: verificar associação entre consumo de álcool com perfil sociodemográfico e alimentação inadequada entre adolescentes escolares. Materiais e métodos: estudo epidemiológico com delineamento transversal, realizado com estudantes de ensino médio. A amostra foi do tipo probabilística por conglomerados. Para a coleta de dados, utilizou-se questionário autoaplicável. A variável dependente foi o consumo de álcool. Foi conduzida Regressão Logística Binária, adotando nível de significância de 5% e as análises foram corrigidas pelo efeito do desenho. Resultados: participaram do estudo 2.040 adolescentes distribuídos em 21 escolas, deles 54,4% (1103) eram do sexo feminino, com quase 60% (1198) dos adolescentes com idade entre 16 a 17 anos. O uso de álcool pelos adolescentes foi referente a 39,1% (774), sendo esse consumo maior entre os meninos (40,3%), quando comparado com as meninas (36,2%). Houve maior probabilidade de consumo de álcool entre os adolescentes que apresentaram maior escolaridade (OR=1.358) e que consumiam refrigerante frequentemente (OR=1.580). Conclusão: neste estudo, o consumo de álcool foi associado com o perfil sociodemográfico e a alimentação inadequada dos adolescentes escolares

Oral Ang-(1-7) treatment improves white adipose tissue remodeling and hypertension in rats with metabolic syndrome.

Objective: Angiotensin (Ang)-(1-7) has preventive effects on metabolic syndrome (MetS). The aim of this study was to evaluate the therapeutic effect of oral Ang-(1-7) on mean arterial pressure (MAP), insulin resistance (IR), inflammatory process, and remodeling of white adipose tissue (WAT) in rats with establishedMetS. Methods: Rats were subjected to control (CT; AIN-93M) or high-fat (HF) diets for 13 wk to induce MetS and treated with Ang-(1-7) or vehicle (V) for the last 6 wk. At the end of 13 wk, MAP, biochemical and histological parameters, and uncoupling protein (UCP) and inflammatory gene expression were determined by quantitative reverse transcription polymerase chain reaction. Results: HF-V rats showed increased visceral fat deposition, inflammatory cytokine expression, hyperplasia, and hypertrophy in retroperitoneal (WAT) and brown adipose tissue (BAT). Additionally, the gastrocnemius muscle reduced UCP-3 and increased the UCP-1 expression in BAT. HF-V also elevated levels of plasma insulin, glucose, homeostatic model assessment (HOMA) of IR and HOMA-b, and increased body mass, adiposity, and MAP. Ang-(1-7) treatment in rats with MetS [HF-Ang-(1-7)] reduced WAT area, number of adipocytes, and expression of proinflammatory adipokines in WAT and BAT and increased UCP-3 in gastrocnemius muscle and UCP-1 expression in BAT compared with the HF-V group. These events prevented body mass gain, reduced adiposity, and normalized fasting plasma glucose, insulin levels, HOMA-IR, HOMA-b, and MAP. Conclusion: Data from the present study demonstrated that oral Ang-(1-7) treatment is effective in restoring biochemical parameters and hypertension in established MetS by improving hypertrophy and hyperplasia in WAT and inflammation in adipose tissue, and regulating metabolic processes in the gastrocnemius muscle and BAT

Antioxidant effects of oral Ang-(1-7) restore insulin pathway and RAS components ameliorating cardiometabolic disturbances in rats.

In prevention studies of metabolic syndrome (MetS), Ang-(1-7) has shown to improve the insulin signaling. We evaluated the HP?CD/Ang-(1-7) treatment on lipid metabolism, renin-angiotensin system (RAS) components, oxidative stress, and insulin pathway in the liver and gastrocnemius muscle and hepatic steatosis in rats with established MetS. After 7 weeks of high-fat (FAT) or control (CT) diets, rats were treated with cyclodextrin (HP?CD) or HP?CD/Ang-(1-7) in the last 6 weeks. FATHP?CD/ empty rats showed increased adiposity index and body mass, gene expression of ACE/ANG II/AT1R axis, and oxidative stress. These results were accompanied by imbalances in the insulin pathway, worsening of liver function, hyperglycemia, and dyslipidemia. Oral HP?CD/Ang-(1-7) treatment decreased ACE and AT1R, increased ACE2 gene expression. in the liver, and restored thiobarbituric acid reactive substances (TBARS), catalase (CAT), superoxide dismutase (SOD), insulin receptor substrate (Irs-1), glucose transporter type 4 (GLUT4), and serine/threonine kinase 2 (AKT-2) gene expression in the liver and gastrocnemius muscle improving hepatic function, cholesterol levels, and hyperglycemia in MetS rats. Overall, HP?CD/Ang-(1-7) treatment restored the RAS components, oxidative stress, and insulin signaling in the liver and gastrocnemius muscle contributing to the establishment of blood glucose and lipid homeostasis in MetS rats

Performance of CMS muon reconstruction in pp collision events at sqrt(s) = 7 TeV

The performance of muon reconstruction, identification, and triggering in CMS has been studied using 40 inverse picobarns of data collected in pp collisions at sqrt(s) = 7 TeV at the LHC in 2010. A few benchmark sets of selection criteria covering a wide range of physics analysis needs have been examined. For all considered selections, the efficiency to reconstruct and identify a muon with a transverse momentum pT larger than a few GeV is above 95% over the whole region of pseudorapidity covered by the CMS muon system, abs(eta) < 2.4, while the probability to misidentify a hadron as a muon is well below 1%. The efficiency to trigger on single muons with pT above a few GeV is higher than 90% over the full eta range, and typically substantially better. The overall momentum scale is measured to a precision of 0.2% with muons from Z decays. The transverse momentum resolution varies from 1% to 6% depending on pseudorapidity for muons with pT below 100 GeV and, using cosmic rays, it is shown to be better than 10% in the central region up to pT = 1 TeV. Observed distributions of all quantities are well reproduced by the Monte Carlo simulation.Comment: Replaced with published version. Added journal reference and DO

Rapid in vivo analysis of synthetic promoters for plant pathogen phytosensing

<p>Abstract</p> <p>Background</p> <p>We aimed to engineer transgenic plants for the purpose of early detection of plant pathogen infection, which was accomplished by employing synthetic pathogen inducible promoters fused to reporter genes for altered phenotypes in response to the pathogen infection. Toward this end, a number of synthetic promoters consisting of inducible regulatory elements fused to a red fluorescent protein (RFP) reporter were constructed for use in phytosensing.</p> <p>Results</p> <p>For rapid analysis, an <it>Agrobacterium</it>-mediated transient expression assay was evaluated, then utilized to assess the inducibility of each synthetic promoter construct <it>in vivo</it>. Tobacco (<it>Nicotiana tabacum </it>cv. Xanthi) leaves were infiltrated with <it>Agrobacterium </it>harboring the individual synthetic promoter-reporter constructs. The infiltrated tobacco leaves were re-infiltrated with biotic (bacterial pathogens) or abiotic (plant defense signal molecules salicylic acid, ethylene and methyl jasmonate) agents 24 and 48 hours after initial agroinfiltration, followed by RFP measurements at relevant time points after treatment. These analyses indicated that the synthetic promoter constructs were capable of conferring the inducibility of the RFP reporter in response to appropriate phytohormones and bacterial pathogens, accordingly.</p> <p>Conclusions</p> <p>These observations demonstrate that the <it>Agrobacterium</it>-mediated transient expression is an efficient method for <it>in vivo </it>assays of promoter constructs in less than one week. Our results provide the opportunity to gain further insights into the versatility of the expression system as a potential tool for high-throughput <it>in planta </it>expression screening prior to generating stably transgenic plants for pathogen phytosensing. This system could also be utilized for temporary phytosensing; e.g., not requiring stably transgenic plants.</p