On-line separation and determination of trivalent and hexavalent chromium with a new liquid membrane annular contactor coupled to inductively coupled plasma optical emission spectrometry

We describe a new on-line sensitive and selective procedure for the determination of trivalent and hexavalent chromium in liquid samples by a tailor-made contactor (TMC), specifically a liquid membrane annular TMC, coupled with inductively coupled plasma with optical detection. The TMC was designed and developed to integrate the extraction and stripping phases of the analyte in one module to minimize the membrane solvent’s consumption and maximize the speed of transport through the liquid membrane. Moreover, the particular geometry studied, which consists of two coaxial hollow fibers, allows the TMC to be used for both separating and preconcentrating purposes. Both (−)-N-dodecyl-N-methylephedrinium bromide (30 mM) in dichloroethane and HNO3 (0.75 M) were used as the liquid membrane and receiving solution, re-spectively. The proposed method’s performance was evaluated in terms of the hexavalent chro-mium extraction efficiency and the coefficient of variation percentages; these were higher than 85% and less than 5%, respectively. In addition, the proposed procedure was applied to two real sam-ples: a tap water sample and an eluate from solid urban waste. In both cases, the analytical per-formances were good and comparable to those obtained using synthetic standard solutions

Bridging aortic valve surgery to 21st century. what can a surgeon do

Aortic valve stenosis is the most clinically relevant valvular heart disease in the elderlies. Surgical aortic valve replacement (SAVR) represented, for decades, the standard of care for the treatment of severe aortic stenosis. Although SAVR still represents a valid option in this clinical scenario, transcatheter aortic valve implantation proved to be superior to medical therapy and comparable to SAVR in several randomized trials in patients at high or intermediate operative risk. At the same time, the growing aging population carrying on greater morbidities and high risk profiles has led to the development of minimally invasive technologies, as rapid deployment aortic valve replacement or Sutureless, to minimize surgical impact on patients. The Heart Team is nowadays tasked to determine the best option tailored for each patient considering patient-related factors and mastering all the surgical options in terms of both different techniques and types of available valves. Nevertheless, some open issues need to be already answered as: which has the longest durability, which the lower complication rate and the lower overall mortality. The aim of this review is to briefly resume the main features of these different options and explore what kind of open questions these newer-generation prosthetic valves and delivery devices carry

Role of Diacylglycerol Kinases in Acute Myeloid Leukemia

: Diacylglycerol kinases (DGKs) play dual roles in cell transformation and immunosurveillance. According to cancer expression databases, acute myeloid leukemia (AML) exhibits significant overexpression of multiple DGK isoforms, including DGKA, DGKD and DGKG, without a precise correlation with specific AML subtypes. In the TGCA database, high DGKA expression negatively correlates with survival, while high DGKG expression is associated with a more favorable prognosis. DGKA and DGKG also feature different patterns of co-expressed genes. Conversely, the BeatAML and TARGET databases show that high DGKH expression is correlated with shorter survival. To assess the suitability of DGKs as therapeutic targets, we treated HL-60 and HEL cells with DGK inhibitors and compared cell growth and survival with those of untransformed lymphocytes. We observed a specific sensitivity to R59022 and R59949, two poorly selective inhibitors, which promoted cytotoxicity and cell accumulation in the S phase in both cell lines. Conversely, the DGKA-specific inhibitors CU-3 and AMB639752 showed poor efficacy. These findings underscore the pivotal and isoform-specific involvement of DGKs in AML, offering a promising pathway for the identification of potential therapeutic targets. Notably, the DGKA and DGKH isoforms emerge as relevant players in AML pathogenesis, albeit DGKA inhibition alone seems insufficient to impair AML cell viability

Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells s

Simple Summary: The aim of this study was to identify new and innovative strategies to improve the tissue-regeneration process. Concentrated growth factor (CGF) is an autologous biomaterial rich in growth factors and multipotent stem cells. The purpose of our study was to evaluate the osteogenic differentiation of CGF primary cells in the presence of a hydroxyapatite–silicon scaffold, which represents a very interesting material in the field of bone reconstructive surgery. Our findings showed that the hydroxyapatite–silicon scaffold provided support to primary CGF cells by enhancing osteogenic differentiation. These data suggest interesting perspectives in the use of CGF together with scaffolds in the field of regenerative medicine. Abstract: The application of scaffolding materials together with stem cell technologies plays a key role in tissue regeneration. Therefore, in this study, CGF (concentrated growth factor), which represents an autologous and biocompatible blood-derived product rich in growth factors and multipotent stem cells, was used together with a hydroxyapatite and silicon (HA-Si) scaffold, which represents a very interesting material in the field of bone reconstructive surgery. The aim of this work was to evaluate the potential osteogenic differentiation of CGF primary cells induced by HASi scaffolds. The cellular viability of CGF primary cells cultured on HA-Si scaffolds and their structural characterization were performed by MTT assay and SEM analysis, respectively. Moreover, the matrix mineralization of CGF primary cells on the HA-Si scaffold was evaluated through Alizarin red staining. The expression of osteogenic differentiation markers was investigated through mRNA quantification by real-time PCR. We found that the HA-Si scaffold was not cytotoxic for CGF primary cells, allowing their growth and proliferation. Furthermore, the HASi scaffold was able to induce increased levels of osteogenic markers, decreased levels of stemness markers in these cells, and the formation of a mineralized matrix. In conclusion, our results suggest that HA-Si scaffolds can be used as a biomaterial support for CGF application in the field of tissue regeneration

mCerulean3-Based Cameleon Sensor to Explore Mitochondrial Ca2+ Dynamics In Vivo

Genetically Encoded Ca2+ Indicators (GECIs) are extensively used to study organelle Ca2+ homeostasis, although some available probes are still plagued by a number of problems, e.g., low fluorescence intensity, partial mistargeting, and pH sensitivity. Furthermore, in the most commonly used mitochondrial F\uf6rster Resonance Energy Transfer based-GECIs, the donor protein ECFP is characterized by a double exponential lifetime that complicates the fluorescence lifetime analysis. We have modified the cytosolic and mitochondria-targeted Cameleon GECIs by (1) substituting the donor ECFP with mCerulean3, a brighter and more stable fluorescent protein with a single exponential lifetime; (2) extensively modifying the constructs to improve targeting efficiency and fluorescence changes caused by Ca2+ binding; and (3) inserting the cDNAs into adeno-associated viral vectors for in vivo expression. The probes have been thoroughly characterized in situ by fluorescence microscopy and Fluorescence Lifetime Imaging Microscopy, and examples of their ex vivo and in vivo applications are described

Brazils Highway BR-319: The road to the collapse of the Amazon and the violation of indigenous rights

One of the greatest threats to the Brazilian Amazon is the reconstruction and paving of the formerly abandoned Highway BR-319, which would link one of the most conserved blocks in the Amazon forest to the “arc of deforestation” on the southern edge of the region where most forest has already been destroyed. BR-319 and its planned side roads would allow the actors and processes from the arc of deforestation to move into vast areas of unprotected rainforest. In the specific case of this highway, a judicial decision that is not subject to further appeal established that environmental studies for the first section of the highway to be reconstructed (“Lot C”) must be carried out before paving. The federal highway department and the “Civil House” of President Bolsonaro’s presidential office ignored this decision and issued a call for bids for the construction work. Due to the current lack of governance in the BR-319 area and the history of deforestation whenever Amazonian highways are built, the decision on whether to suspend the contract for the “Lot C” is critical for the maintenance of both the ecosystem services of the Amazon forest and the way of life of indigenous and riverside people. This decision is expected to be made shortly by a single person

Factors Associated With Severe Gastrointestinal Diagnoses in Children With SARS-CoV-2 Infection or Multisystem Inflammatory Syndrome

Importance Severe gastrointestinal (GI) manifestations have been sporadically reported in children with COVID-19; however, their frequency and clinical outcome are unknown. Objective To describe the clinical, radiological, and histopathologic characteristics of children with COVID-19 presenting with severe GI manifestations to identify factors associated with a severe outcome. Design, Setting, and Participants A multicenter retrospective cohort study (February 25, 2020, to January 20, 2021) enrolled inpatient and outpatient children (aged <18 years) with acute SARS-CoV-2 infection, confirmed by positive real-time reverse-transcriptase–polymerase chain reaction on nasopharyngeal swab or fulfilling the US Centers for Disease Control and Prevention criteria for multisystem inflammatory syndrome in children (MIS-C). The study was conducted by pediatricians working in primary care or hospitals in Italy participating in the COVID-19 Registry of the Italian Society of Pediatric Infectious Diseases. Main Outcomes and Measures The occurrence of severe GI manifestations, defined by a medical and/or radiological diagnosis of acute abdomen, appendicitis (complicated or not by perforation and/or peritonitis), intussusception, pancreatitis, abdominal fluid collection, and diffuse adenomesenteritis requiring surgical consultation, occurring during or within 4 to 6 weeks after infection with SARS-CoV-2 infection. Logistic regression was used to estimate odds ratios (ORs) with 95% CIs of factors potentially associated with severe outcomes. Results Overall, 685 children (386 boys [56.4%]; median age, 7.3 [IQR, 1.6-12.4] years) were included. Of these children, 628 (91.7%) were diagnosed with acute SARS-CoV-2 infection and 57 (8.3%) with MIS-C. The presence of GI symptoms was associated with a higher chance of hospitalization (OR, 2.64; 95% CI, 1.89-3.69) and intensive care unit admission (OR, 3.90; 95% CI, 1.98–7.68). Overall, 65 children (9.5%) showed severe GI involvement, including disseminated adenomesenteritis (39.6%), appendicitis (33.5%), abdominal fluid collection (21.3%), pancreatitis (6.9%), or intussusception (4.6%). Twenty-seven of these 65 children (41.5%) underwent surgery. Severe GI manifestations were associated with the child’s age (5-10 years: OR, 8.33; 95% CI, 2.62-26.5; >10 years: OR, 6.37; 95% CI, 2.12-19.1, compared with preschool-age), abdominal pain (adjusted OR [aOR], 34.5; 95% CI, 10.1-118), lymphopenia (aOR, 8.93; 95% CI, 3.03-26.3), or MIS-C (aOR, 6.28; 95% CI, 1.92-20.5). Diarrhea was associated with a higher chance of adenomesenteritis (aOR, 3.13; 95% CI, 1.08-9.12) or abdominal fluid collection (aOR, 3.22; 95% CI, 1.03-10.0). Conclusions and Relevance In this multicenter cohort study of Italian children with SARS-CoV-2 infection or MIS-C, 9.5% of the children had severe GI involvement, frequently associated with MIS-C. These findings suggest that prompt identification may improve the management of serious complications

Infliximab in paediatric inflammatory bowel disease

AbstractInfliximab has been widely used in paediatric Crohn's disease, mainly in luminal and fistulous disease refractory to standard treatment and for extraintestinal manifestations. Moreover, there is growing experience with its use in refractory ulcerative colitis. Infliximab has shown similar efficacy and safety in children as in adult population. It is postulated that its early use in paediatric inflammatory bowel disease, as a bridging treatment until the onset of action of other immunomodulators, could reduce the use of steroids and change the natural history of the disease as well. The effect of infliximab on mucosal healing could also contribute to the normal growth and sexual maturation in these patients

Entrevistas a los ex integrantes de los Consejos de Redacción de Lecciones y Ensayos (1956-2016)

Fil: Ortiz, Tulio. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Teoría del Estado-Profesor Emérito. Buenos Aires, ArgentinaFil: Díaz de Vivar, Elisa Matilde. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Derecho Civil I. Buenos Aires, ArgentinaFil: Dulitzky, Ariel. Universidad de Buenos Aires. Facultad de Derecho.Cátedra Derechos Humanos. Buenos Aires, ArgentinaFil: Dulitzky, Ariel. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Derecho Constitucional. Buenos Aires, ArgentinaFil: Ferrante, Marcelo. Universidad de Buenos Aires. Facultad de Derecho. Buenos Aires, ArgentinaFil: Bloch, Ivana Verónica. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Elementos de Derecho Penal y Procesal Penal. Buenos Aires, ArgentinaFil: Bergallo, Paola. CONICET-Universidad de Buenos Aires. Facultad de Derecho. Buenos Aires, ArgentinaFil: Filippini, Leonardo G. Universidad de Buenos Aires. Facultad de Derecho. Centro de Estudios de Ejecución Penal (CEEP). Buenos Aires, ArgentinaFil: Sigal, Martín. Universidad de Buenos Aires. Facultad de Derecho. Centro de Derechos Humanos (CDH). Buenos Aires, ArgentinaFil: Bloch, Demise. Universidad de Buenos Aires. Facultad de Derecho. Buenos Aires, ArgentinaFil: Freedman, Diego. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Finanzas Públicas y Derecho Tributario. Buenos Aires, ArgentinaFil: Pezzot, Romina. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Derecho Internacional Público. Buenos Aires, ArgentinaFil: Hopp, Cecilia. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Derecho Administrativo. Buenos Aires, ArgentinaFil: Rojas, Mishkila. Universidad de Buenos Aires. Facultad de Derecho. Proyectos DeCyT. Buenos Aires, ArgentinaFil: Garaventa, Carlos A. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Derecho Público II. Buenos Aires, ArgentinaFil: Kenny, Patricio Enrique. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Teoría General del Derecho. Buenos Aires, ArgentinaFil: Ramallo, María de los Ángeles. Universidad de Buenos Aires. Facultad de Derecho. Centro de Derechos Humanos (CDH). Buenos Aires, ArgentinaFil: Furfaro, Lautaro. Universidad de Buenos Aires. Facultad de Derecho; ArgentinaFil: Piqué, María Luisa. Universidad de Buenos Aires. Facutad de Derecho. Cátedra Elementos de Derecho Constitucional. Buenos Aires, ArgentinaFil: Benente, Mauro. Universidad de Buenos Aires. Facultad de Derecho. Instituto de Investigaciones Jurídicas y Sociales Ambrosio L. Gioja. Buenos Aires, ArgentinaFil: Etchegorry, María Alejandra. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Derecho Internacional Privado. Buenos Aires, ArgentinaFil: Monti, Ezequiel. Universidad de Buenos Aires. Facultad de Derecho. Teoría General y Filosofía del Derecho. Buenos Aires, ArgentinaFil: Green Martínez, Sebastián. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Derecho Internacional Público. Buenos Aires, ArgentinaFil: Bulit Goñi, Magdalena. Universidad de Buenos Aires. Facultad de Derecho. Buenos Aires, ArgentinaFil: Brodsky, Jonathan Matías. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Obligaciones Civiles y Comerciales. Buenos Aires, ArgentinaFil: Olivera, Federico Eduardo. Universidad de Buenos Aires. Facultad de Derecho. Proyecto UBACyT. Buenos Aires, Argentin

European registry of type A aortic dissection (ERTAAD) - rationale, design and definition criteria

Correction: Volume16 Issue1 Article Number225 DOI10.1186/s13019-021-01606-8Background: Acute Stanford type A aortic dissection (TAAD) is a life-threatening condition. Surgery is usually performed as a salvage procedure and is associated with significant postoperative early mortality and morbidity. Understanding the patient's conditions and treatment strategies which are associated with these adverse events is essential for an appropriate management of acute TAAD. Methods: Nineteen centers of cardiac surgery from seven European countries have collaborated to create a multicentre observational registry (ERTAAD), which will enroll consecutive patients who underwent surgery for acute TAAD from January 2005 to March 2021. Analysis of the impact of patient's comorbidities, conditions at referral, surgical strategies and perioperative treatment on the early and late adverse events will be performed. The investigators have developed a classification of the urgency of the procedure based on the severity of preoperative hemodynamic conditions and malperfusion secondary to acute TAAD. The primary clinical outcomes will be in-hospital mortality, late mortality and reoperations on the aorta. Secondary outcomes will be stroke, acute kidney injury, surgical site infection, reoperation for bleeding, blood transfusion and length of stay in the intensive care unit. Discussion: The analysis of this multicentre registry will allow conclusive results on the prognostic importance of critical preoperative conditions and the value of different treatment strategies to reduce the risk of early adverse events after surgery for acute TAAD. This registry is expected to provide insights into the long-term durability of different strategies of surgical repair for TAAD.Peer reviewe