Genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matrices

Journal of Osseointegration Volume 7, Issue 1, 1 March 2015, Pages 2-7 Open Access Genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matrices (Article) Pozzuoli, A.a, Gardin, C.b, Aldegheri, R.a, Bressan, E.c, Isola, M.d, Calvo-Guirado, J.L.e, Biz, C.a, Arrigoni, P.a, Feroni, L.b, Zavan, B.b a Department of Surgical,Oncological and Gastroenterological Sciences, University of Padua, Padua, Italy b Department of Biomedical Sciences, University of Padua, Padua, Italy c Department of Neurosciences, University of Padua, Padua, Italy d Department of Animal Medicine, Production and Health (MAPS), Italy e Department of General Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain Hide additional affiliations View references (44) Abstract Aim: Tissue engineering is a rapidly expanding field with regard to the use of biomaterials and stem cells in the orthopedic surgery. Many experimental studies have been done to understand the best characteristics of cells, materials and laboratory methods for safe clinical applications. The aim of this study was to compare the ability of 2 different human demineralized bone matrices (DBMs), the one enriched and the other not enriched with hyaluronic acid, to stimulate in vitro the proliferation and the osteogenic differentiation of human adipose-derived stem cells (ADSCs) seeded onto an osteoconductive scaffold. Materials and Methods: ADSCs were isolated, by enzymatic digestion, from abdominal adipose tissue of 5 patients undergoing cosmetic lipoaspiration surgery. ADSCs were then seeded onto a 3D scaffold in the presence of the two different osteoinductive matrices of human demineralized bone and evaluated for proliferation and osteogenic differentiation. The safety of the methods was verified using array-Comparative Genomic Hybridization (array-CGH). Results: ADSCs were able to differentiate in osteogenic sense. Both DBMs showed the ability to induce osteogenic differentiation of the cells. Conclusion: array-CGH showed no changes at genome level, thus confirming the safety of materials and method

Polyphase folding at upper structural levels in the Borbera valley (Northern Apennines, Italy): implications for the tectonic evolution of the linkage area between Alps and Apennines

The Borbera Valley (northwestern Italy) is located in a complex geological area where the linkage between Alps and Apennines occurs. In this area the Antola Unit (Late Cretaceous–Palaeocene) is unconformably overlain by the Upper Eocene–Miocene succession of the Tertiary Piedmont Basin. The structural analysis indicates the occurrence of a folding phase of Late Oligocene–Early Miocene age, characterised by recumbent F2 folds. These folds are superposed onto D1 structures related to an early folding phase of Middle Eocene, affecting only the Antola Unit. The occurrence of map-scale D2 folding phase structures that affect the Tertiary Piedmont Basin succession suggests that the linkage area between Alps and Apennines was reactivated during the Late Oligocene–Early Miocene

Contrasting styles of (U)HP rock exhumation along the Cenozoic Adria-Europe plate boundary (Western Alps, Calabria, Corsica)

Since the first discovery of ultrahigh pressure (UHP) rocks 30 years ago in the Western Alps, the mechanisms for exhumation of (U)HP terranes worldwide are still debated. In the western Mediterranean, the presently accepted model of synconvergent exhumation (e.g., the channel-flow model) is in conflict with parts of the geologic record. We synthesize regional geologic data and present alternative exhumation mechanisms that consider the role of divergence within subduction zones. These mechanisms, i.e., (i) the motion of the upper plate away from the trench and (ii) the rollback of the lower plate, are discussed in detail with particular reference to the Cenozoic Adria-Europe plate boundary, and along three different transects (Western Alps, Calabria-Sardinia, and Corsica-Northern Apennines). In the Western Alps, (U)HP rocks were exhumed from the greatest depth at the rear of the accretionary wedge during motion of the upper plate away from the trench. Exhumation was extremely fast, and associated with very low geothermal gradients. In Calabria, HP rocks were exhumed from shallower depths and at lower rates during rollback of the Adriatic plate, with repeated exhumation pulses progressively younging toward the foreland. Both mechanisms were active to create boundary divergence along the Corsica-Northern Apennines transect, where European southeastward subduction was progressively replaced along strike by Adriatic northwestward subduction. The tectonic scenario depicted for the Western Alps trench during Eocene exhumation of (U)HP rocks correlates well with present-day eastern Papua New Guinea, which is presented as a modern analog of the Paleogene Adria-Europe plate boundary

Facies analysis of the Late Quaternary deposits along the coast between Livorno and Piombino: paleoenvironmental and neotectonic implications

The coast between Livorno and Piombino (Southern Tuscany) displays geological evidences of quaternary tectono-eustatic interaction. Through an accurate review of previous works and a detailed geological survey, 116 sites where outcrop Late Pleistocene deposits have been located. Facies analysis allowed the identification of 11 different lithostratigraphic units, constituting a larger number than the 3 or 4 units recognized in previous studies (Cortemiglia et al., 1983; Hearty & Dai Pra, 1987; Costantini et al., 1993 and Mauz, 1999).They are constituted by mainly sandstone levels formed in upper shoreface to foreshore/backshore and coastal dune environments, separated by silty and sandy pedogenized continental deposits. In particular, aeolian deposits have been documented in this area, on the basis of sedimentological features (e.g. presence of contorted bedding, pin stripe laminae, convex-upward stratification) and fossiliferous evidences (oligotipycal non-marine mollusc fauna, Caprid track-ways). A stratigraphical correlation sketches, based on recent chronostratigraphic data (Hearty & Dai Pra, 1987; Mauz, 1999), have been proposed for the study area and, compared with the last 130 ky global sea-level curves, suggest a tectono-eustatic interaction. The superposition of the 11 lithostratigraphic units laid between MIS5 and Holocene (MIS1), associated with their depositional environment interpretation, implies repeated phases of subsidence followed by a very recent uplift. Coastal sectors with differential subsidence/uplift rates have also been recognized along the study area. These data are in strong conflict with other neotectonic reconstructions, based on MIS 5.5 markers (Nisi et al., 2003; Ferranti et al., 2006), which suggest stability or gentle uplift for this area

The Romagna Appennines, Italy: an eroded duplex

The study of clast composition carried out on the alluvial gravels of the Romagna Apennines of northern Italy has provided evidence for an extensive covering of allochthonous units (Ligurian nappe and Epiligurian succession) above the Miocene fore-deep deposits (Marnoso-Arenacea Formation), which has been subsequently eroded during the Late Miocene-Pleistocene uplift. This result is confirmed by the burial history outlined in the Marnoso-Arenacea Formation through vitrinite reflectance and apatite fission-track analyses. The Romagna Apennines represent, therefore, a regional tectonic window where the thrust system that displaced the Marnoso-Arenacea Formation crops out. The geometric relations between this thrust system and the basal thrust of the Ligurian nappe, exposed at the boundaries of the Romagna Apennines (Sillaro Zone and Val Marecchia klippe), are consistent with a duplex structure. Thus, the Romagna Apennines thrust system is an eroded duplex. The duplex roof-thrust corresponds to the surface of the synsedimentary overthrust of the Ligurian nappe on the Marnoso-Arenacea Formation; the floor-thrust is located in the pelagic pre-foredeep deposits (Schlier Formation)