Resistance of endodontically treated roots restored with different fibre post systems with or without post space preparation: in vitro analysis and SEM investigation.

Aim: To compare the mechanical resistance to fracture of two conical post systems placed with no preparation of the root canal with that of double taper fibre posts seated in endodontically treated single roots after standard post space preparation using dedicated drills. Methodology: Thirty fibre posts with double (G1, n = 10, DT Light Post) and single taper (G2, n = 10, SurgiPost Multiconical; G3, n = 10, Tech ES Endoshape) were luted with self-adhesive cement in endodontically treated single roots using different post space preparation techniques. The bonded posts were experimentally loaded until failure and the maximum load to fracture was registered. Fracture patterns were qualitatively evaluated and SEM analysis was performed to assess the quality of endodontic treatments and cementation. Data were statistically analysed by means of one-way ANOVA. Results: The mean maximum load to fracture was 165.05 23.46 N in G1, 151.52 16.23 N in G2 and 129.09 15.25 N in G3. Statistically significant differences were pointed out between G1 and G3 (p < 0.01) and G2 and G3 (p < 0.05). No root fractures were evidenced. SEM analyses showed slightly thicker cement ayers at the apical and middle thirds of single taper posts (G2 and G3). Conclusions: DT Light Post and SurgiPost Multiconical fibre posts showed similar properties in terms of mechanical resistance to fracture and higher than those of Tech ES Endoshape. Unrestorable root fractures did not occur with any of the tested posts

Search for 14.4 keV solar axions from M1 transition of Fe-57 with CUORE crystals

We report the results of a search for axions from the 14.4 keV M1 transition from Fe-57 in the core of the sun using the axio-electric effect in TeO2 bolometers. The detectors are 5x5x5 cm3 crystals operated at about 10 mK in a facility used to test bolometers for the CUORE experiment at the Laboratori Nazionali del Gran Sasso in Italy. An analysis of 43.65 kg d of data was made using a newly developed low energy trigger which was optimized to reduce the detectors energy threshold. An upper limit of 0.63 c kg-1 d-1 was established at 95% C.L.. From this value, a lower bound at 95% C.L. was placed on the Peccei-Quinn energy scale of fa >= 0.76 10**6 GeV for a value of S=0.55 for the flavor-singlet axial vector matrix element. Bounds are given for the interval 0.15 < S < 0.55.Comment: 14 pages, 6 figures, submitted to JCA

Validation of techniques to mitigate copper surface contamination in CUORE

In this article we describe the background challenges for the CUORE experiment posed by surface contamination of inert detector materials such as copper, and present three techniques explored to mitigate these backgrounds. Using data from a dedicated test apparatus constructed to validate and compare these techniques we demonstrate that copper surface contamination levels better than 10E-07 - 10E-08 Bq/cm2 are achieved for 238U and 232Th. If these levels are reproduced in the final CUORE apparatus the projected 90% C.L. upper limit on the number of background counts in the region of interest is 0.02-0.03 counts/keV/kg/y depending on the adopted mitigation technique.Comment: 10 pages, 6 figures, 6 table

The Brazilian Registry of Adult Patient Undergoing Cardiovascular Surgery, the BYPASS Project: Results of the First 1,722 Patients

Objective: To report the early results of the BYPASS project - the Brazilian registrY of adult Patient undergoing cArdiovaScular Surgery - a national, observational, prospective, and longitudinal follow-up registry, aiming to chart a profile of patients undergoing cardiovascular surgery in Brazil, assessing the data harvested from the initial 1,722 patients. Methods: Data collection involved institutions throughout the whole country, comprising 17 centers in 4 regions: Southeast (8), Northeast (5), South (3), and Center-West (1). The study population consists of patients over 18 years of age, and the types of operations recorded were: coronary artery bypass graft (CABG), mitral valve, aortic valve (either conventional or transcatheter), surgical correction of atrial fibrillation, cardiac transplantation, mechanical circulatory support and congenital heart diseases in adults. Results: 83.1% of patients came from the public health system (SUS), 9.6% from the supplemental (private insurance) healthcare systemsand 7.3% from private (out-of-pocket) clinic. Male patients comprised 66%, 30% were diabetics, 46% had dyslipidemia, 28% previously sustained a myocardial infarction, and 9.4% underwent prior cardiovascular surgery. Patients underwent coronary artery bypass surgery were 54.1% and 31.5% to valve surgery, either isolated or combined. The overall postoperative mortality up to the 7th postoperative day was 4%for CABG was 2.6%, and for valve operations, 4.4%. Conclusion: This first report outlines the consecution of the Brazilian surgical cardiac database, intended to serve primarily as a tool for providing information for clinical improvement and patient safety and constitute a basis for production of research protocols.Univ Fed Sao Paulo UNIFESP EPM, Hosp Sao Paulo, Sao Paulo, SP, BrazilHosp Caridade Sao Vicente Paulo, Jundiai, SP, BrazilInst Med Integral Prof Fernando Figueira IMIP, Recife, PE, BrazilHosp Base FUNFARME & FAMERP, Sao Jose Do Rio Preto, SP, BrazilIMC, Sao Jose Do Rio Preto, SP, BrazilIrmandade Santa Casa Sao Paulo INCT HPV, Fac Ciencias Med Santa Casa Sao Paulo, Sao Paulo, SP, BrazilFundacao Univ Cardiol, Inst Cardiol Rio Grande do Sul, Porto Alegre, RS, BrazilInst Coracao Natal, Natal, RN, BrazilInst Cardiol Dist Fed, Brasilia, DF, BrazilUniv Fed Maranhao HU UFMA, Univ Hosp, Sao Luis, MA, BrazilHosp Evangelico, Cachoeiro De Itapemirim, ES, BrazilHosp Coracao Sergipe, Aracaju, SE, BrazilHosp Nossa Senhora Salete, Inst Cirurgia Cardiovasc ICCV, Cascavel, PR, BrazilHosp Wilson Rosado, Mossoro, RN, BrazilHosp Bosque Saude, Sao Paulo, SP, BrazilHosp Univ Santa Maria, Santa Maria, RS, BrazilHosp Coracao HCor, Sao Paulo, SP, BrazilHosp Coracao IP HCor, Ins Pesquisa, Sao Paulo, SP, BrazilInst Coracao InCor, Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP EPM, Hosp Sao Paulo, Sao Paulo, SP, BrazilWeb of Scienc

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer‐reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state‐of‐the‐art handbook for basic and clinical researchers.DFG, 389687267, Kompartimentalisierung, Aufrechterhaltung und Reaktivierung humaner Gedächtnis-T-Lymphozyten aus Knochenmark und peripherem BlutDFG, 80750187, SFB 841: Leberentzündungen: Infektion, Immunregulation und KonsequenzenEC/H2020/800924/EU/International Cancer Research Fellowships - 2/iCARE-2DFG, 252623821, Die Rolle von follikulären T-Helferzellen in T-Helferzell-Differenzierung, Funktion und PlastizitätDFG, 390873048, EXC 2151: ImmunoSensation2 - the immune sensory syste

Le calque et l'écran. « Genèse architecturale et nouveaux médias ». Entretien

Das Vordringen der neuen Technologien in die Architekturbüros hat die Schaffensbedingungen der Architekten grundlegend veràndert : Die virtuelle Schicht und das graphische EDV-Programm haben Durchschlagpapier und Zeichenstift ersetzt, Bildschirme den Zeichentisch, die digitalisierte Form der Plane und hat den für die Archivierung notigen Biiroraum durch zwei geteilt ; Beziehungen von Konzeption, Schreiben und Zeichnung wurden neu definiert, bildlicher Darstellungsraum hat sich auf Maße des Bildschirms und auf genormte Formate der Drucker reduziert. Aber die langsame Erstellung der Endfassung konnte nur einer schwerfàlligen Dateneingabe weichen, die Maschinen verleiten zu mechanischen Fehlern usw. Arbeitsgeschwindigkeit, Reflektionsvorgànge und Projektierungstechniken àndern sich. Die früheren Tràger (Modellbau, Zeichenhefte, Durchschlàge) behalten eine beschrànkte aber entscheidende Stelle im Konzeptionsprozess.A entrada das novas tecnologias nos ateliers alterou completamente as condicoes da criacao em arquitectura. O piano virtual e a programacao grafica substituiram o papel vegetal e o lapis, os monitores substituiram as mesas de desenhador e o arquivo de plantas tornouse numerico, reduzindo para metade o espaco das instalacoes. As relacoes entre concepcao, escrita e desenho foram redefinidas, o espaco da representacao fechouse nas dimensoes do monitor e do formato estandardizado das impressoras. Mas o lento processo de reproducao por copia dos documentos cedeu o lugar a uma pesada acumulacao de dados ; as maquinas induzem em perigosos automatismos, etc. A velocidade do trabalho, os processos de reflexao e as tecnicas de projectar alteramse. Os antigos suportes (maquetes, cadernos de esbocar, decalques) mantem um um papel reduzido mas decisivo no processo de concepcao.The eruption of new technologies into the studios has brought sweeping changes to the conditions of architectural creation : virtual overlays and graphics programs take over from tracing paper and pencils, screens replace drawing tables, and blueprint archives go digital, cutting office space in half. The relations between conception, writing, and drawing are redefined as the space of representation shrinks to fit the size of the monitor and the standardized formats of printers, but the slow production of final copies has only given way to cumbersome data-entry, with machines inducing the risk of automatism, etc. Working speed, procedures of reflection, and project techniques are all in transformation. Nevertheless the old media (scale models, sketch pads, tracing paper) retain a diminished but decisive role in the design process.L'irruzione delle nuove tecnologie negli studi di architettura ha rivoluzionato le condizioni della creazione architettonica : la componente virtuale e il software grafico hanno preso il posto délia carta copiativa e della matita, gli schermi hanno sostituito i tavoli da disegno e l'archiviazione dei progetti è stata digitalizzata, dimezzando gli spazi dello studio, i rapporti tra concezione, scrittura e disegno si sono ridefiniti, lo spazio délia rappresentazione si è concentrato nella misura dello schermo e nei formati standard delle stampanti ; ma la lenta messa a fuoco dei documenti è scomparsa per lasciare il posto a una complessa registrazione di dati, le macchine inducono dei rischi di automatismi ecc. Velocità di lavoro, procedure di riflessione e tecniche di progettazione si trasformano. I vecchi strumenti (modelli in scala, quaderni di schizzi, calco) hanno un posto ridotto ma decisivo nei processi creativi.L'irruption des nouvelles technologies dans les agences a bouleversé les conditions de la création architecturale : la couche virtuelle et le logiciel graphique se sont substitués au papier calque et au crayon, les écrans ont remplacé les tables à dessin et l'archivage des plans est devenu numérique en divisant par deux l'espace de l'agence ; les relations entre conception, écriture et dessin se sont redéfinies, l'espace de la représentation s'est concentré sur la taille de l'écran et les formats standardisés des imprimantes, mais la lente mise au net des documents n'a disparu qu'au profit d'une lourde saisie des données, les machines induisent des risques d'automatismes, etc. Vitesse de travail, procédures de réflexion et techniques de projet se transforment. Les anciens supports (maquettes, carnets de croquis, calque) gardent une place réduite mais décisive dans le processus de conception.La irrupcion de las nuevas tecnologias en las agencias ha transformado las condiciones de la creacion arquitectural : la capa virtual y el programa grafico han reemplazado al papel de calcar y al lapiz ; las mesas de dibujo han sido sustituidas por las pantallas y el archivo de los pianos ha pasado a ser numerico reduciendo por la mitad el espacio de la agencia ; las relaciones entre concepcion, escritura y dibujo han debido ser redefinidas ; el espacio de la representation se ha ajustado a las dimensiones de la pantalla y al formato estandar de las impresoras, pero si la tarea laboriosa de puesta en limpio de los documentos ha desaparecido, su lugar a sido ocupado por la captura masiva de datos, por los riesgos de automatimatismos que traen aparejados las maquinas, etc. La velocidad de trabajo, los procedimientos de reflexion y las tecnicas de proyecto se ven transformados. Los antiguos soportes (maquetas, libretas de croquis, calcos) conservan un lugar redu-cido, aunque decisivo, en el proceso de conception.Montès Fernando, Biasi Pierre-Marc de. Le calque et l'écran. « Genèse architecturale et nouveaux médias ». Entretien. In: Genesis (Manuscrits-Recherche-Invention), numéro 14, 2000. Architecture, sous la direction de Pierre-Marc de Biasi et Réjean Legault. pp. 129-152

Clinical and radiographic evaluation of canine stifle joint after surgical stabilization for the treatment of cranial cruciate ligament rupture: retrospective study of three years<br>Avaliação clínica e radiográfica do joelho de cães submetidos à cirurgia para correção da ruptura do ligamento cruzado cranial: estudo retrospectivo de três anos

This study aimed a clinical and radiographic evaluation of stifle joint of dogs with up to two and a half years of postoperative correction of cranial cruciate ligament rupture. A retrospective study of three years has been done, based on the animals files treated at the Hospital Veterinário da Universidade Estadual de Londrina, underwent this surgery during the period from February 2008 to February 2011. Totalizing 28 cases and 33 stifle joints, which were performed different surgical techniques for joint stabilization. The prevalence was female, middle aged over seven years and dogs weighing over 25 kg. Eighty-two percent of the animals showed satisfactory clinical improvement, independent of surgical technique. Despite the progression of arthrosis and the persistence of discrete drawer movement in the postoperative period, no evident clinical differences were noted in relation to lameness. Lighter animals showed better prognosis. There was no evident difference between the surgical techniques in relation to the parameters evaluated in the postoperative period. <P><P> O presente trabalho objetivou avaliar clínica e radiograficamente o joelho de cães com até dois anos e meio de pós-operatório da correção da ruptura de ligamento cruzado cranial. Realizou-se um estudo retrospectivo de três anos dos prontuários dos animais atendidos no Hospital Veterinário da Universidade Estadual de Londrina, submetidos a esta cirurgia no período compreendido entre fevereiro de 2008 a fevereiro de 2011. Totalizando 28 casos e 33 joelhos, nos quais foram realizadas diferentes técnicas cirúrgicas para a estabilização articular. A prevalência foi de fêmeas, média de idade superior a sete anos e cães com peso acima de 25 kg. Oitenta e dois por cento dos animais apresentaram melhora clínica satisfatória, independente da técnica cirúrgica utilizada. Apesar da progressão da artrose e da persistência de discreto movimento de gaveta no pós-operatório, não houve diferença clínica evidente com relação ao grau de claudicação. Animais mais leves apresentaram prognóstico melhor. Não ocorreu diferença significativa entre as técnicas cirúrgicas com relação aos parâmetros avaliados neste período pós-operatório