Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the developing male Wistar(Han) rat II: chronic dosing causes developmental delay

We have investigated whether fetal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes defects in the male reproductive system of the rat, using chronically exposed rats to ensure continuous exposure of the fetus. 5-6 week old rats were exposed to control diet, or diet containing TCDD, to attain an average dose of 2.4, 8 and 46 ng TCDD kg-1 day-1 for twelve weeks, whereupon the rats were mated, and allowed to litter; rats were switched to control diet after parturition. Male offspring were allowed to develop until kills on PND70 (25 per group), or PND120 (all remaining animals). Offspring from the high dose group showed an increase in total litter loss, and the number of animals alive on post-natal day (PND) 4 in the high dose group was ~26% less than control. The high and medium dose offspring showed decreased weights at various ages. Balano-preputial separation was significantly delayed in all three dose groups, compared to control. There were no significant effects of maternal treatment when the offspring were subjected to a functional observational battery, or learning tests, with the exception that the high dose group showed a deficit in motor activity. 20 rats per group were mated to females, and there were no significant effects of maternal treatment on the fertility of these rats, nor on the F1 or F2 sex ratio. Sperm parameters at PND70 and 120 showed no significant effect of maternal treatment, with the exception that there was an increase in the proportion of abnormal sperm in the high dose group at PND70; this is associated with the developmental delay in puberty in this dose group. There were no remarkable findings of maternal treatment on organ weights, with the exception that testis weights were reduced by ~10% at PND70 (but not PND120), and although the experiment was sufficiently powered to detect small changes, ventral prostate weight was not reduced. There were no significant effects of maternal treatment upon histopathological comparison of high dose and control group organs. These data confirm that developmental exposure to TCDD shows no potent effect on adult sperm parameters or accessory sexual organs, but show that delay in BPS occurs after exposure to low doses of TCDD, and this is dependent upon whether TCDD is administered acutely or chronically

Microwave plasma chemical vapour deposition diamond nucleation on ferrous substrates with Ti and Cr interlayers

Diamond-coated steel is considered an important issue in synthetic diamond technology due to the great economical importance of enhancing the wear resistance and surface hardness of commercial Fe-based alloys. However, direct diamond coating by chemical vapour deposition (CVD) is rather problematic-adhesion and growth are seriously affected. The use of interlayers is a common approach to minimise these problems. This work reports an investigation on the establishment of good nucleation and growth conditions of diamond films by microwave plasma CVD (MPCVD) on ferrous substrates coated with Ti and Cr interlayers. Commercial grade ferrous substrates were pre-coated with commercial interlayers by sputtering (Ti, Cr) and electroplating (Cr) techniques. Steel substrates led to better results than iron cast substrates. The best films were obtained on Ti pre-coated steel substrate. The results on Cr interlayers pointed to the advantage of electroplating over the physical vapour deposition (PVD) sputtering. From the two selected parameter sets for diamond deposition, the one using lower power level conducted to the best results. Initial roughness and growth parameters were found to counteract on the uniformity of the diamond films. The morphology was studied by scanning electron microscopy (SEM), the roughness was estimated by profilometry, while diamond quality and stress state were evaluated by mu-Raman spectroscopy. (C) 2002 Elsevier Science B.V. All rights reserved

Long-term optical photometric monitoring of the quasar SDSS J153259.96-003944.1

We report optical Cousins R and I band monitoring observations of the high redshift (z = 4.67) QSO SDSS J153259.96-003944.1 that does not show detectable emission lines in its optical spectrum. We show this object varies with a maximum amplitude of ~0.4 mag during a year and three months of monitoring. Combined with two other epochs of photometric data available in the literature, we show the object has gradually faded by ~0.9 mag during the period June 1998 - April 2001. A linear least squares fit to all available observations gives a slope of ~0.35 mag/yr which translates to ~1.9 mag/yr in the rest frame of the quasar. Such a variability is higher than that typically seen in QSOs but consistent with that of BL Lacs, suggesting that the optical continuum is Doppler boosted. Alternatively, within photometric errors, the observed lightcurve is also consistent with the object going through a microlensing event. Photoionization model calculations show the mass of the Broad Line Region to be few tens of solar mass similar to that of low luminosity Seyfert galaxies, but about orders of magnitude less than that of luminous quasars. Further frequent photometric/spectroscopic monitoring is needed to support or refute the different alternatives discussed here on the nature of SDSS J153259.96-003944.1.Comment: 7 pages with 4 figures, uses mn2e.cls, Accepted for publication in MNRA

Modeling and simulation of high-pressure industrial autoclave polyethylene reactor

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)High-pressure technology for polyethylene production has been widely used by industries around the world. A good model for the reactor fluid dynamics is essential to set the operating conditions of an autoclave reactor. The high-pressure autoclave reactor model developed in this work was based on a non-isothermal dynamic model, where PID control equations are used to maintain the operation at the unstable steady state. The kinetic mechanism to describe the polymerization rate and molecular weight averages are presented. The model is capable of computing temperature, concentration gradients and polymer characteristics. The model was validated for an existing industrial reactor and data for production of homopolymer polyethylene and has represented well the behavior of the autoclave reactor used in ethylene homopolymerization.215764PolitenoFinepConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Transplantation of embryonic spleen tissue reveals a role for adult non-lymphoid cells in initiating lymphoid tissue organization

In this report we describe a transplantation system where embryonic spleens are grafted into adult hosts. This model can be used to analyze the cellular and molecular requirements for the development and organization of splenic microenvironments.Whole embryonic day 15 (ED15) spleens, grafted under the kidney capsule of adult mice, were colonized by host-derived lymphocytes and DC and developed normal splenic architecture. Grafts were also able to form germinal centers in response to T-dependent antigen. Using this system we demonstrated that adult host-derived lymphotoxin (LT) a was sufficient for the development of ED15 LTa/ grafts. Grafting of ED15 LTa/ spleens into RAG/ hosts followed by transfer of LT a/ splenocytes revealed no requirement for lymphocyte-derived LT a in the induction of CCL21 or the development of T-zone stroma. These data suggest that interactions between adult lymphoid-tissue inducer-like cells and embryonic stromal cells initiated T-zone development. Furthermore,adult lymphoid tissue inducer-like cells were shown to develop from bone marrow-derived progenitors. The model described here demonstrates a method of transferring whole splenic microenvironments and dissecting the stromal and hematopoietic signals involved in spleen development and organization

A QSO host galaxy and its Lyalpha emission at z=6.43

We report an optical detection of an extended structure around a QSO at z=6.43 (CFHQSJ2329-0301, the highest redshift QSO currently known) in deep z' and z_r-band images of the Subaru/Suprime-Cam. After a careful PSF (QSO) subtraction, a structure in the z'-band extends more than 4'' on the sky (R_e=11 kpc), and thus, is well-resolved (16sigma detection). The PSF-subtracted z_r-band structure is in a similar shape to that in the z'-band, but less significant with a 3 sigma detection. In the z'-band, a radial profile of the QSO+host shows a clear excess over that of the averaged PSF in 0.8-3'' radius. Since the z'-band includes a Lya emission at z=6.43, the z' flux is perhaps a mixture of the host (continuum light) and its Lya emission, whereas the z_r-band flux is from the host. Through a SED modeling, we estimate 40% of the PSF-subtracted z'-band light is from the host (continuum) and 60% is from Lya emission. The absolute magnitude of the host is M_{1450}=-23.9 (c.f. M_{1450}=-26.4 for the QSO). A lower limit of the SFR(Lya) is 1.6 Msun yr^{-1} with stellar mass ranging 6.2 x 10^8 to 1.1 x 10^10 Msun when 100 Myrs of age is assumed. The detection shows that a luminous QSO is already harbored by a large, star-forming galaxy in the early Universe only after ~840 Myr after the big bang. The host may be a forming giant galaxy, co-evolving with a super massive black hole.Comment: Accepted for publication in MNRAS. A higher resolution pdf is at http://ifa.hawaii.edu/~tomo/QSOhost/QSOhost_v7.pd

Policy and performance in customs: Evaluating the trade facilitation agreement

This is the author accepted manuscript. The final version is available from Wiley via the DOI in this record.This paper links cross‐country variation in trade facilitation policies to prominent indicators of national customs and logistics performance. We test the hypotheses that the policy data explain variation in the performance indicators, independent of other country characteristics that are plausibly exogenous to changes in trade facilitation policies. We use a LASSO procedure to choose among the many potential explanatory variables in the model. In general we find that country characteristics related to geography, income, and the general quality of governance better explain cross‐country variation in customs and logistics performance than do the measures of trade facilitation policy.Government of SwedenGovernment of NorwayGovernment of the United Kingdo

Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the developing male Wistar(Han) rat II: chronic dosing causes developmental delay

We have investigated whether fetal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes defects in the male reproductive system of the rat, using chronically exposed rats to ensure continuous exposure of the fetus. 5-6 week old rats were exposed to control diet, or diet containing TCDD, to attain an average dose of 2.4, 8 and 46 ng TCDD kg-1 day-1 for twelve weeks, whereupon the rats were mated, and allowed to litter; rats were switched to control diet after parturition. Male offspring were allowed to develop until kills on PND70 (25 per group), or PND120 (all remaining animals). Offspring from the high dose group showed an increase in total litter loss, and the number of animals alive on post-natal day (PND) 4 in the high dose group was ~26% less than control. The high and medium dose offspring showed decreased weights at various ages. Balano-preputial separation was significantly delayed in all three dose groups, compared to control. There were no significant effects of maternal treatment when the offspring were subjected to a functional observational battery, or learning tests, with the exception that the high dose group showed a deficit in motor activity. 20 rats per group were mated to females, and there were no significant effects of maternal treatment on the fertility of these rats, nor on the F1 or F2 sex ratio. Sperm parameters at PND70 and 120 showed no significant effect of maternal treatment, with the exception that there was an increase in the proportion of abnormal sperm in the high dose group at PND70; this is associated with the developmental delay in puberty in this dose group. There were no remarkable findings of maternal treatment on organ weights, with the exception that testis weights were reduced by ~10% at PND70 (but not PND120), and although the experiment was sufficiently powered to detect small changes, ventral prostate weight was not reduced. There were no significant effects of maternal treatment upon histopathological comparison of high dose and control group organs. These data confirm that developmental exposure to TCDD shows no potent effect on adult sperm parameters or accessory sexual organs, but show that delay in BPS occurs after exposure to low doses of TCDD, and this is dependent upon whether TCDD is administered acutely or chronically

Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the developing male Wistar(Han) rat I: no decrease in epididymal sperm count after a single acute dose

It has been reported that fetal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes defects in the male reproductive system of the rat. We set out to replicate and extend these effects using a robust experimental design. Groups of 75 (control vehicle) or 55 (50, 200 or 1000 ng of TCDD kg-1 bodyweight) female Wistar(Han) rats were exposed to TCDD on Gestational Day (GD) 15, then allowed to litter. The high dose group dams showed no sustained weight loss compared to control, but four animals had total litter loss. Pups in the high dose group showed reduced body weight up till day 21, and pups in the medium dose group showed reduced body weight in the first week post partum. Balano-preputial separation (BPS) was significantly delayed in the high dose group male offspring. There were no significant effects of treatment when the offspring were subjected to a functional observational battery, or mated with females to assess reproductive capability. 25 males per group were killed on post natal day (PND) 70, and ~60 animals per group (~30 for the high dose group) on PND120 to assess seminology and other endpoints. At PND120, the two highest dose groups showed a statistically significant elevation of sperm counts, compared to control; however, this effect was small (~30%), within the normal range of sperm counts for this strain of rat, was not reflected in testicular spermatid counts nor PND70 data, and is therefore postulated to have no biological significance. Although there was an increase in the proportion of abnormal sperm at PND70, seminology parameters were otherwise unremarkable. Testis weights in the high dose group were slightly decreased at PND 70 and 120, and at PND120, brain weights were decreased in the high dose group, liver to body weight ratios were increased for all three dose groups, with an increase in inflammatory cell foci in the epididymis in the high dose group. These data show that TCDD is a potent developmental toxin after exposure of the developing fetus, but that acute developmental exposure to TCDD on GD15 caused no decrease in sperm counts