Time management of sport managers in fitness centers

El objetivo de este trabajo es conocer el tiempo que dedican a las diferentes tareas laborales los directores deportivos o máximos coordinadores deportivos (DDCC) en centros de fitness (CCFF) privados según las dimensiones de la instalación, el número de socios, y la experiencia del responsable. Para ello se empleó una muestra de 37 DDCC (5 mujeres y 32 hombres) con edades superiores a los 21 años, de CCFF privados del municipio de Sevilla, los cuales respondieron un cuestionario específico para este estudio. Los resultados obtenidos indican una relación entre el aumento de la superficie de las instalaciones y una mayor dedicación en tareas de gestión administrativa, gestión de RRHH (recursos humanos) y reuniones. Aumento del número de socios, con más dedicación en tareas de marketing, gestión administrativa, gestión de RRHH, reuniones y planificación de nuevos servicios. Y por último se observó que a mayor experiencia, mayor es la dedicación a reuniones y labores de técnico

El uso de las redes sociales y el engagement de los clubes de la Liga Endesa ACB

Las redes sociales se han convertido en una plataforma idónea para dar a conocer y aumentar la visibilidad de una marca o empresa y también, para interactuar y analizar las acciones de los fans y seguidores de estas. Debido a ello, el presente estudio pretende averiguar las diferentes redes sociales que emplean los clubes de baloncesto de la Liga Endesa ACB, examinar el Engagement de Facebook y Twitter y, analizar si existe una correlación entre la posición en el ranking al final de la liga, número de victorias, años desde la fundación del club y aforo de espectadores a sus estadios, con el Engagement en Facebook y Twitter. Participaron la totalidad de clubes de la Liga Endesa ACB (18 clubes de baloncesto) de la temporada 2015-2016, utilizando la hoja de observación, y posterior análisis descriptivo y de regresión lineal múltiple. Los resultados indican el uso por parte de todos los clubes de Facebook y Twitter, y como tercera red social de YouTube. Los tres primeros equipos en el ranking mostraron los valores más elevados de Engagement en Facebook, y los dos primeros y el noveno en el Engagement en Twitter. Finalmente, el número de victorias resultó ser predictor del Engagement en Facebook y Twitter. Aunque el número de estudios referentes a las redes sociales se están incrementando, estos hallazgos muestran la necesidad de seguir analizando posibles variables predictoras del Engagement, para con ello, poder determinar variables que puedan repercutir en el compromiso y la relación de los fans y seguidores de los clubes de baloncesto.

Aplicación de la teoría de la generalizabilidad en la evaluación de servicios en gestión deportiva

This paper addresses from the use of the Generalizability Theory (TG) the optimization of resources in sports management. We used a design structure composed of the facets users (U), activities (A), scales (E) and items (I), following not orthogonal design, partially nested and multifaceted, within the General Linear Model. 537 participants (32.11 ± 11.11 years) completed the Questionnaire of Evaluation of the Perceived Quality in Sports Services obtaining excellent results both indexes of reliability (ξρ²(δ) > 0.98) and generalizability (ξρ²(Δ) > 0.98). The optimization showed that half of participants and fewer activities, we obtain a high degree of generalization and at the same time, reduced the total number of observations, being important for future studies which will facilitate the evaluation of sport service more efficiently.Esta investigación aborda a partir de la Teoría de la Generalizabilidad (TG) la optimización de recursos en la gestión deportiva. Se utilizó una estructura de diseño compuesta por las facetas usuarios (U), actividades (A), escalas (E) e ítems (I), siguiendo un diseño no ortogonal, parcialmente anidado y multifaceta dentro del Modelo General Lineal (GLM). Los 537 participantes (32.11 ± 11.11 años) cumplimentaron el Cuestionario de Evaluación de la Calidad Percibida en Servicios Deportivos obteniendo resultados excelentes de fiabilidad (ξρ²(δ) > 0.98) y generalizabilidad (ξρ²(Δ) > 0.98). La optimización mostró que con una reducción del número de participantes a la mitad y con un menor número de actividades evaluadas, obtenemos un alto grado de generalización al mismo tiempo que se reduce el número total de observaciones de cara a futuros registros, lo que facilitará la evaluación del servicio deportivo de manera más eficiente

Fabrication of human myocardium using multidimensional modelling of engineered tissues

Biofabrication of human tissues has seen a meteoric growth triggered by recent technical advancements such as human induced pluripotent stem cells (hiPSCs) and additive manufacturing. However, generation of cardiac tissue is still hampered by lack of adequate mechanical properties and crucially by the often unpredictable post-fabrication evolution of biological components. In this study we employ melt electrowriting (MEW) and hiPSC-derived cardiac cells to generate fibre-reinforced human cardiac minitissues. These are thoroughly characterized in order to build computational models and simulations able to predict their post-fabrication evolution. Our results show that MEW-based human minitissues display advanced maturation 28 post-generation, with a significant increase in the expression of cardiac genes such as MYL2, GJA5, SCN5A and the MYH7/MYH6 and MYL2/MYL7 ratios. Human iPSC-cardiomyocytes are significantly more aligned within the MEW-based 3D tissues, as compared to conventional 2D controls, and also display greater expression of C ×43. These are also correlated with a more mature functionality in the form of faster conduction velocity. We used these data to develop simulations capable of accurately reproducing the experimental performance. In-depth gauging of the structural disposition (cellular alignment) and intercellular connectivity (C ×43) allowed us to develop an improved computational model able to predict the relationship between cardiac cell alignment and functional performance. This study lays down the path for advancing in the development of in silico tools to predict cardiac biofabricated tissue evolution after generation, and maps the route towards more accurate and biomimetic tissue manufacture

Risk profiles and one-year outcomes of patients with newly diagnosed atrial fibrillation in India: Insights from the GARFIELD-AF Registry.

BACKGROUND: The Global Anticoagulant Registry in the FIELD-Atrial Fibrillation (GARFIELD-AF) is an ongoing prospective noninterventional registry, which is providing important information on the baseline characteristics, treatment patterns, and 1-year outcomes in patients with newly diagnosed non-valvular atrial fibrillation (NVAF). This report describes data from Indian patients recruited in this registry. METHODS AND RESULTS: A total of 52,014 patients with newly diagnosed AF were enrolled globally; of these, 1388 patients were recruited from 26 sites within India (2012-2016). In India, the mean age was 65.8 years at diagnosis of NVAF. Hypertension was the most prevalent risk factor for AF, present in 68.5% of patients from India and in 76.3% of patients globally (P < 0.001). Diabetes and coronary artery disease (CAD) were prevalent in 36.2% and 28.1% of patients as compared with global prevalence of 22.2% and 21.6%, respectively (P < 0.001 for both). Antiplatelet therapy was the most common antithrombotic treatment in India. With increasing stroke risk, however, patients were more likely to receive oral anticoagulant therapy [mainly vitamin K antagonist (VKA)], but average international normalized ratio (INR) was lower among Indian patients [median INR value 1.6 (interquartile range {IQR}: 1.3-2.3) versus 2.3 (IQR 1.8-2.8) (P < 0.001)]. Compared with other countries, patients from India had markedly higher rates of all-cause mortality [7.68 per 100 person-years (95% confidence interval 6.32-9.35) vs 4.34 (4.16-4.53), P < 0.0001], while rates of stroke/systemic embolism and major bleeding were lower after 1 year of follow-up. CONCLUSION: Compared to previously published registries from India, the GARFIELD-AF registry describes clinical profiles and outcomes in Indian patients with AF of a different etiology. The registry data show that compared to the rest of the world, Indian AF patients are younger in age and have more diabetes and CAD. Patients with a higher stroke risk are more likely to receive anticoagulation therapy with VKA but are underdosed compared with the global average in the GARFIELD-AF. CLINICAL TRIAL REGISTRATION-URL: http://www.clinicaltrials.gov. Unique identifier: NCT01090362

A utilização de redes sociais e o Engagement dos clubes na Liga Endesa ACB

Las redes sociales se han convertido en una plataforma idónea para dar a conocer y aumentar la visibilidad de una marca o empresa y también, para interactuar y analizar las acciones de los fans y seguidores de estas. Debido a ello, el presente estudio pretende averiguar las diferentes redes sociales que emplean los clubes de baloncesto de la Liga Endesa ACB, examinar el Engagement de Facebook y Twitter y, analizar si existe una correlación entre la posición en el ranking al fnal de la liga, número de victorias, años desde la fundación del club y aforo de espectadores a sus estadios, con el Engagement en Facebook y Twitter. Participaron la totalidad de clubes de la Liga Endesa ACB (18 clubes de baloncesto) de la temporada 2015-2016, utilizando la hoja de observación, y posterior análisis descriptivo y de regresión lineal múltiple. Los resultados indican el uso por parte de todos los clubes de Facebook y Twitter, y como tercera red social de YouTube. Los tres primeros equipos en el ranking mostraron los valores más elevados de Engagement en Facebook, y los dos primeros y el noveno en el Engagement en Twitter. Finalmente, el número de victorias resultó ser predictor del Engagement en Facebook y Twitter. Aunque el nú- mero de estudios referentes a las redes sociales se están incrementando, estos hallazgos muestran la necesidad de seguir analizando posibles variables predictoras del Engagement, para con ello, poder determinar variables que puedan repercutir en el compromiso y la relación de los fans y seguidores de los clubes de baloncesto.Abstract: Social networks have become an ideal platform to raise awareness and increase the visibility of a brand or company and also to interact and analyze the actions of the fans and followers of these ones. Because of this, the present study aims to fnd out the diferent social networks which are employed by the basketball clubs Endesa ACB League, and examine if the use of Facebook and Twitter, have a correlation, in terms of Engagement, with the position in the ranking at the end of the league, number of wins, years since the founding of the club and attendance of spectators in stadiums. All League clubs Endesa ACB (18 basketball clubs) of the 2015-2016 season were researched by using an observation sheet, and subsequent descriptive and multiple linear regression analysis. Te results indicate the use, by all the clubs, of Facebook and Twitter, and a third social network called YouTube. Te top three teams in the rankings showed the highest values of Engagement on Facebook, and the frst two and the ninth in the Engagement on Twitter. Lastly, the number of victories of a team seemed to be the predictor of Engagement on Facebook and Twitter. Although the number of studies on social networks are increasing, these fndings show the need to further analyze possible predicting factors of Engagement, to thereby be able to determine which variables may have an efect in the commitment and the relationship of fans and followers of basketball clubs.Resumo: As redes sociais tornaram-se uma plataforma idónea para sensibilizar e aumentar a visibilidade de uma marca, de uma empresa e também para interagir e analisar as ações dos fãs e seguidores. No seguimento, o presente estudo tem como objetivo investigar as diferentes redes sociais utilizadas pelos clubes de basquetebol da Liga Endesa ACB, analisando o envolvimento com Facebook e o Twitter e se existe uma correlação entre a posição no ranking no fnal do campeonato, número de vitórias, anos desde a fundação do clube e número de espetadores nos estádios, com o envolvimento no Facebook e no Twitter. Participaram no estudo todos os clubes da Liga Endesa ACB (18 clubes de basquetebol) na temporada 2015- 2016, utilizando-se para o estudo a fcha de observação, e posteriormente, a análise descritiva e a regressão linear múltipla. Os resultados indicam que todos os clubes usam o Facebook e Twitter e, como uma terceira rede social, o YouTube. As três melhores equipas do ranking apresentaram os maiores valores de envolvimento com o Facebook, e os dois primeiros e o nono do ranking um envolvimento com o Twitter. Finalmente, o número de vitórias provou ser um indicador de envolvimento com o Facebook e com o Twitter. Embora o número de estudos sobre as redes sociais tenha vindo a aumentar, estes resultados demostram a necessidade de uma análise mais aprofundada dos possíveis indicadores de envolvimento, para assim ser possível determinar variáveis que possam afetar o envolvimento e a relação de fãs e seguidores de clubes de basquetebol

The structural basis for signal promiscuity in a bacterial chemoreceptor

Signalling through chemosensory pathways is typically initiated by the binding of signal molecules to the chemoreceptor ligand binding domain (LBD). The PcaY_PP chemoreceptor from Pseudomonas putida KT2440 is characterized by an unusually broad signal range, and minimal requisites for signal binding are the presence of a C6-membered ring and that of a carboxyl group. Previous studies have shown that only some of the multiple signals recognized by this chemoreceptor are of apparent metabolic value. We report here high-resolution structures of PcaY_PP-LBD in the absence and presence of four cognate chemoeffectors and glycerol. The domain formed a four-helix bundle (4HB), and both ligand binding sites of the dimer were occupied with the high-affinity ligands protocatechuate and quinate, whereas the lower-affinity ligands benzoate and salicylate were present in only one site. Ligand binding was verified by microcalorimetric titration of site-directed mutants revealing important roles of an arginine and number of polar residues that establish an extensive hydrogen bonding network with bound ligands. The comparison of the apo and holo structures did not provide evidence for this receptor employing a transmembrane signalling mechanism that involves piston-like shifts of the final helix. Instead, ligand binding caused rigid-body scissoring movements of both monomers of the dimer. Comparisons with the 4HB domains of the Tar and Tsr chemoreceptors revealed significant structural differences. Importantly, the ligand binding site in PcaY_PP-LBD is approximately 8 Å removed from that of the Tar and Tsr receptors. Data indicate a significant amount of structural and functional diversity among 4HB domains. Databases: The coordinates and structure factors have been deposited in the protein data band with the following IDs: 6S1A (apo form), 6S18 (bound glycerol), 6S33 (bound protocatechuate), 6S38 (bound quinate), 6S3B (bound benzoate) and 6S37 (bound salicylate).We greatly appreciate the provision of beam time at ALBA and ESRF and the support of the beam linestaff during data collection. This work was supported by FEDER funds and Fondo Social Europeo through grants from the Spanish Ministry for Science, Innovation and Universities to MAM (PID2019-103972GA-I00), and the Spanish Ministry of Economy and Competitiveness to JAG (BIO2016-74875-P) and TK(BIO2016-76779-P

The structural basis for signal promiscuity in a bacterial chemoreceptor

Signalling through chemosensory pathways is typically initiated by the binding of signal molecules to the chemoreceptor ligand binding domain (LBD). The PcaY_PP chemoreceptor from Pseudomonas putida KT2440 is characterized by an unusually broad signal range, and minimal requisites for signal binding are the presence of a C6-membered ring and that of a carboxyl group. Previous studies have shown that only some of the multiple signals recognized by this chemoreceptor are of apparent metabolic value. We report here high-resolution structures of PcaY_PP-LBD in the absence and presence of four cognate chemoeffectors and glycerol. The domain formed a four-helix bundle (4HB), and both ligand binding sites of the dimer were occupied with the high-affinity ligands protocatechuate and quinate, whereas the lower-affinity ligands benzoate and salicylate were present in only one site. Ligand binding was verified by microcalorimetric titration of site-directed mutants revealing important roles of an arginine and number of polar residues that establish an extensive hydrogen bonding network with bound ligands. The comparison of the apo and holo structures did not provide evidence for this receptor employing a transmembrane signalling mechanism that involves piston-like shifts of the final helix. Instead, ligand binding caused rigid-body scissoring movements of both monomers of the dimer. Comparisons with the 4HB domains of the Tar and Tsr chemoreceptors revealed significant structural differences. Importantly, the ligand binding site in PcaY_PP-LBD is approximately 8 Å removed from that of the Tar and Tsr receptors. Data indicate a significant amount of structural and functional diversity among 4HB domains. Databases: The coordinates and structure factors have been deposited in the protein data band with the following IDs: 6S1A (apo form), 6S18 (bound glycerol), 6S33 (bound protocatechuate), 6S38 (bound quinate), 6S3B (bound benzoate) and 6S37 (bound salicylate).We greatly appreciate the provision of beam time at ALBA and ESRF and the support of the beam linestaff during data collection. This work was supported by FEDER funds and Fondo Social Europeo through grants from the Spanish Ministry for Science, Innovation and Universities to MAM (PID2019-103972GA-I00), and the Spanish Ministry of Economy and Competitiveness to JAG (BIO2016-74875-P) and TK(BIO2016-76779-P

Production of cross-linked lipase crystals at a preparative scale

[EN] The autoimmobilization of enzymes via cross-linked enzyme crystals (CLECs) has regained interest in recent years, boosted by the extensive knowledge gained in protein crystallization, the decrease of cost and laboriousness of the process, and the development of potential applications. In this work, we present the crystallization and preparative-scale production of reinforced cross-linked lipase crystals (RCLLCs) using a commercial detergent additive as a raw material. Bulk crystallization was carried out in 500 mL of agarose media using the batch technique. Agarose facilitates the homogeneous production of crystals, their cross-linking treatment, and their extraction. RCLLCs were active in an aqueous solution and in hexane, as shown by the hydrolysis of p-nitrophenol butyrate and α-methylbenzyl acetate, respectively. RCLLCs presented both high thermal and robust operational stability, allowing the preparation of a packed-bed chromatographic column to work in a continuous flow. Finally, we determined the three-dimensional (3D) models of this commercial lipase crystallized with and without phosphate at 2.0 and 1.7 Å resolutions, respectively.The work was supported by the project BIO2016-74875-P (MINECO), Spain, co-funded by the Fondo Europeo de Desarrollo Regional (FEDER funds), European Union. The authors are grateful to the Spanish Radiation Synchrotron source (ALBA), Barcelona, Spain, and the European Synchrotron Radiation Facility (ESRF), Grenoble, France, for the provision of time and the staff at XALOC (Alba) and ID30B and ID30A-3 (ESRF) beamlines for assistance during data collection.Peer reviewe