Solar water disinfection (SODIS): Impact on hepatitis A virus and on a human Norovirus surrogate under natural solar conditions

This study evaluates the effectiveness of solar water disinfection (SODIS) in the reduction and inactivation of hepatitis A virus (HAV) and of the human Norovirus surrogate, murine Norovirus (MNV-1), under natural solar conditions. Experiments were performed in 330 ml polyethylene terephthalate (PET) bottles containing HAV or MNV-1 contaminated waters (103 PFU/ml) that were exposed to natural sunlight for 2 to 8 h. Parallel experiments under controlled temperature and/or in darkness conditions were also included. Samples were concentrated by electropositive charged filters and analysed by RT-real time PCR (RT-qPCR) and infectivity assays. Temperature reached in bottles throughout the exposure period ranged from 22 to 40ºC. After 8 h of solar exposure (cumulative UV dose of ~828 kJ/m2 and UV irradiance of ~20 kJ/l), the results showed significant (P < 0.05) reductions from 4.0 (±0.56) ×104 to 3.15 (±0.69) × 103 RNA copies/100 ml (92.1%, 1.1 log) for HAV and from 5.91 (±0.59) × 104 to 9.24 (±3.91) × 103 RNA copies/100 ml (84.4%, 0.81 log) for MNV-1. SODIS conditions induced a loss of infectivity between 33.4% and 83.4% after 4 to 8 h in HAV trials, and between 33.4% and 66.7% after 6 h to 8 h in MNV-1 trials. The results obtained indicated a greater importance of sunlight radiation over the temperature as the main factor for viral reduction. [Int Microbiol 2015; 18(1):41-49]Keywords: Solar water disinfection (SODIS) · water disinfection · hepatitis A virus (HAV) · murine Norovirus (MNV-1

La comunicación digital del sector oleícola. El caso de las almazaras de la provincia de Jaén

Recent events such as COVID-19 and the conflict in Ukraine have increased the de-mand for olive oil, a key product in the Spanish agri-food system due to its history and foreign trade. Jaén accounts for 37% of national production. Through content analysis, the websites of the 74 olive oil mills in this province were investigated to identify the distinctive elements of their digital communication and the way they connect with their audiences. The results indicate that this is a traditional and con-servative sector that is starting to go digital, mainly through its presence on social networks.Recientes acontecimientos como la COVID-19 y el conflicto en Ucrania han aumentado la demanda del aceite de oliva, producto clave en el sistema agroalimentario español por su legado y comercio exterior. Jaén acumula el 37% de la producción a nivel nacional. A través del análisis de contenido se investigan las páginas webs de las 74 almazaras de esta provincia para identificar los elementos distintivos de su comunicación digital y el modo de conectar con sus públicos. Los resultados indican que es un sector tradicional y conservador que empieza a apostar por lo digital, fundamentalmente mediante la presencia en redes sociales

Personal branding and politics: analysis of Isabel Díaz Ayuso and Yolanda Díaz’s communication on Facebook

Isabel Díaz Ayuso, President of the Community of Madrid, and Yolanda Díaz, Second Vice-President of the Government and Minister of Labor and Social Economy, are two of the most influential Spanish politicians when it comes to female political leadership in Spain (Top 100, 2021; Yo Dona, 2021, 2022) because of their social significance. Both have strengthened their communication strategy on social networks and especially on Facebook, where they regularly provide agenda setting content that is also of interest to citizens. This research carries out an exhaustive quantitative and qualitative content analysis of all the posts published on this digital platform by both female leaders during the first six months of 2022. The results underline their use of Facebook to strengthen their personal brand while they tend to disassociate themselves from political symbolism, which emphasizes, in this context, their tendencies towards personalization through individual leadership and their humanization as political figures. Taking a distinctive approach, Yolanda Díaz opts for relaxed body language and cheerful facial expressions, while Isabel Díaz Ayuso is faithful to her traditional reservedness and seriousness when it comes to gestural communication. Both focus their messages towards citizens and the fulfilment of their electoral programs.Isabel Díaz Ayuso, presidenta de la Comunidad de Madrid, y Yolanda Díaz, vicepresidenta segunda del Gobierno y ministra de Trabajo y Economía Social, son dos de las políticas españolas consideradas más influyentes en cuanto a liderazgo político femenino en España (Top 100, 2021; Yo Dona, 2021, 2022) por su significación social. Ambas han fortalecido su estrategia de comunicación en redes sociales y especialmente en Facebook, donde asiduamente facilitan contenido de interés para la agenda setting y la ciudadanía. Esta investigación realiza un exhaustivo análisis de contenido cuantitativo y cualitativo de la totalidad de los posts publicados en esta plataforma digital por ambas mandatarias durante los seis primeros meses del año 2022. Los resultados subrayan el empleo de Facebook para fortalecer su marca personal al mismo tiempo que tienden a desvincularse de simbología política, enfatizando en su contexto las tendencias a la personalización mediante el liderazgo individual y la humanización de sus figuras. Desde un enfoque claramente diferenciador, Yolanda Díaz opta por una postura corporal siempre relajada y facciones alegres, mientras que Isabel Díaz Ayuso es fiel a sus tradicionales sobriedad y seriedad en comunicación gestual. Ambas focalizan sus mensajes en la ciudadanía y el cumplimiento de sus programas electorales

Efecto de distintas fertilizaciones de fósforo en la resistencia de brinzales de encina y alcornoque a "Phytophthora cinnamomi" Rands

El factor de contribución más virulento en el proceso de decaimiento forestal en el sur de la península ibérica es el Oomiceto Phytophthora cinnamomi Rands. Recientes investigaciones avalan el uso de fertilizaciones fosfóricas y fosfitos como fungicida contra Phytophthora cinnamomi. El objetivo general de este trabajo es estudiar el efecto de la fertilización fosfórica en brinzales de Quercus ilex y Q. suber sobre la resistencia a la podredumbre radical producida por P. cinnamomi. Se realizó un ensayo con brinzales de encina y alcornoque que previamente habían sido cultivados con un programa de fertilización fosfórica, dando lugar a cuatro tratamientos: Fosfato-A (3 mg P2O5 por planta), Fosfato-B (6 mg de P2O5 por planta), Fosfito (0,15 mg de P2O5 por planta) y no fertilizado. El ensayo se realizó en invernadero y tuvo una duración de 7 meses. Consistió en inocular las plantas de los distintos tratamientos con Phytophthora cinnamomi Rands. y estudiar la evolución de sus atributos morfológicos (altura, diámetro del cuello de la raíz y biomasa de raíz secundaria) y fisiológicos (contenido foliar de nutrientes). Los resultados mostraron que la fertilización con fosfatos no había mejorado la resistencia a la podredumbre radical causada por P. cinnamomi, mientras que el fosfito logró controlar totalmente la infección del patógeno, dando resultados negativos el reaislamiento de la especie fúngica inoculada en este tratamiento. Las plantas tratadas con fosfito presentaron un estado morfológico y fisiológico igual, y en algunos aspectos mejor, que el Control no inoculado. Se puede concluir que sería posible lograr una acción protectora frente a P. cinnamomi mediante el tratamiento con fosfitos durante el cultivo en vivero.The oomycete Phytophthora cinnamomi Rands is the mean responsible for oak decline in southern Spain. This paper investigates the relationship between phosphoric fertilization of Quercus ilex and Q. suber seedlings and resistance to the disease caused by Phytophthora cinnamomi. The study was conducted on holm oak and cork oak seedlings previously submitted to a phosphoric fertilization programme involving four different treatments, namely: Phosphate-A (3 mg P2O5 seedling), Phosphate-B (6 mg P2O5 seedling), Phosphite (0.15 mg de P2O5 seedling) and No Fertilization. Tests were conducted in greenhouse over a period of 7 months and a number of treatments to plants inoculated with Phytophthora cinnamomi Rands. were applied, assessing the changes in their morphological (viz. height, root and collar diameter, and secondary root biomass) and physiological characteristics (viz. leaf nutrient contents). Based on the results, phosphate failed to improve plant resistance to P. cinnamomi; by contrast, phosphite successfully avoided infection by this pathogen. In fact, the phosphitetreated plants exhibited a morphological and physiological status similar to or even better than that of the non-inoculated controls. The protective effect of phosphite against P. cinnamomi can be achieved by applying it during seedling cultivation in nurseries

Urban wastewater disinfection for agricultural reuse: Effect of solar driven AOPs in the inactivation of a multidrug resistant E. coli strain

The occurrence of antibiotics in urban wastewater treatment plants (UWTPs) may result in the development of antibiotic resistance and subsequently in the release of multidrug resistant bacteria (MDR) and genes into the effluent. Conventional disinfection processes are only partially effective in controlling ARB spread, so advanced oxidation processes (AOPs) have been investigated as alternative option in this work. In particular, the aim of this work was to comparatively assess the efficiency of solar disinfection and solar driven AOPs (namely H2O2/sunlight, TiO2/sunlight, H2O2/TiO2/sunlight, natural photo-Fenton) for the inactivation of a multidrug (namely ampicillin, ciprofloxacin and tetracycline) resistant E. coli strain isolated from the effluent of the biological process of an UWTP. Different concentrations of H2O2 (0.588–1.470–2.205 mM), TiO2 (50–100 mg L−1), H2O2/TiO2 (0.147 mM/50 mg L−1, 0.588 mM/100 mg L−1) and Fe2+/H2O2 (0.090/0.294, 0.179/0.588, 0.358/1.176 mM) were evaluated at pilot-scale (in compound parabolic collector reactor) in real biologically treated wastewater. All investigated processes resulted in a complete inactivation (5-log decrease) of bacteria until detection limit, but the best disinfection efficiency in terms of treatment time (20 min to reach the detection limit) and required energy (0.98 kJ L−1) was observed for photo-Fenton at pH 4 (Fe2+/H2O2:0.090/0.294 mM). Antimicrobial susceptibility was tested by Kirby-Bauer disk diffusion method. Ampicillin and ciprofloxacin (to which the selected strain is resistant), cefuroxime and nitrofurantoin were chosen as tested antibiotics. None of the investigated processes affected antibiotic resistance of survived colonies

Extrusion of Ingot and Powder Metallurgy Aluminum Matrix Composites Profiles

Several profiles with the same extrusion ratio were extruded from three monolithic alloys and five aluminum matrix composites (AMCs) prepared by ingot and powder metallurgy (PM). Specifically, AA6061 and AA6063 were reinforced by 10 and 25 vol.% of Al2O3 and MoSi2 intermetallic particles. To study the wear of the dies, high temperature pin-on-disk tests were performed. The results show that the composite profiles present high surface and dimensional quality and that the die steel wears slightly more against the cast composite. Maximum extrusion pressure (Pmax) depends on profile shape and increases in the following order: bar, T, L and U, and tube. It also increases as volume fraction of reinforcement increases. In general, cast materials are easier to extrude than PM ones, and MoSi2 reinforced AMCs are easier to extrude than Al2O3 ones. AMCs processed by PM have significantly higher hardness than the cast AMC and the monolithic alloys. The PM composites do not need any heat treatment to acquire maximum hardness. By adding reinforcing particles to aluminum, a significant increase in tensile strength and stiffness occur. The high tensile strength and modulus of the present PM composites, without a decrease in specific properties, makes these AMCs interesting for structural applications.Spanish project TRACE2009_0251 from MICINN, Spain, and EXTRUGASA, Galicia, Spain, are gratefully acknowledged. Thanks are also due to Amalia San Román and Miguel Acedo for their help with the experimental work.Peer reviewe

Social networks as a marketing tool: comparative analysis in the case of Dulceida

Today, social networks are emerging as platforms that redefine the marketing strategies of advertisers through influencers. This research studies the figure of Dulceida, a fashion and beauty influencer, through content analysis of advertising activity on her Instagram profile and her YouTube channel during 2018. The results underline the strength of her personal brand in her prescription work, with different trends and potentialities appreciated by both platforms.Actualmente las redes sociales se erigen como plataformas que redefinen las estrategias de marketing de los anunciantes mediante los influencers. Esta investigación estudia la figura de Dulceida, influencer de moda y belleza, a través del análisis de contenido de la actividad publicitaria en su perfil de Instagram y su canal de YouTube durante 2018. Los resultados subrayan la fuerza de su marca personal en su labor de prescripción, apreciándose diferentes tendencias y potencialidades por parte de ambas plataformas

Murciano-Granadina goat performance and methane emission after replacing barley grain with fibrous by-products

[ES] El objetivo de este trabajo fue estudiar los efectos de la sustitución de grano de cebada en la dieta por pulpa de naranja o vainas de soja en el balance de nitrógeno y carbono, las emisiones de metano y el rendimiento de la producción de leche en cabras. Para ello, se seleccionaron doce cabras lecheras Murciano-Granadina y se dividieron en tres grupos basados en un peso corporal similar (42,1 ± 1,2 kg) y la producción de leche (2,16 ± 0,06 kg/cabra/día). El experimento se realizó en un diseño cruzado donde un grupo de cuatro cabras fue alimentado con una ración mixta de grano de cebada, en otro grupo de cuatro cabras se reemplazó el grano de cebada por pulpa de naranja y el último grupo de cuatro cabras se alimentó con soja. Después de la adaptación a las dietas, las cabras se establecieron en jaulas individuales y se analizaron las heces, la orina y la leche. Asimismo, las mediciones de intercambio gaseoso fueron registradas por un sistema móvil de calorimetría en circuito abierto. El consumo de materia seca fue similar en los tres grupos (2,03 kg/d, en promedio). No se observó ninguna influencia de la dieta en el balance energético. Las dietas basadas en pulpa de naranja y cascarilla de soja mostraron una mayor movilización de grasa que en el caso de grano de cebada. El ácido pentadecanoico y el ácido heptadecanoico fueron potenciales biomarcadores de la función del rumen debido a los contenidos más elevados encontrados en la leche de cabras con la dieta de pulpa de naranja y vainas de soja frente al detectado en las cabras con dieta de grano de cebada, lo que sugiere un impacto de estas dietas sobre el metabolismo bacteriano rumen. Esto probablemente esté relacionado con un suministro de nitrógeno inferior en el caso de la dieta de pulpa de naranja para sintetizar proteína microbiana y un mayor contenido de grasa en el caso de la dieta de cascarilla de soja. La sustitución de grano de cereal con subproductos fibrosos no aumentó las emisiones de metano (54,7 L/cabra por día, en promedio). Por lo tanto, se podría utilizar pulpa de naranja y cascarilla de soja en la dieta de las cabras lactantes sin tener un impacto negativo en el rendimiento de la producción de leche.[EN] The aim of this experiment was to study the effects of substituting dietary barley grain with orange pulp or soybean hulls on energy, nitrogen and carbon balance, methane emission and milk performance in dairy goats. Twelve Murciano-Granadina dairy goats in midlactation were selected and divided into three groups based on similar body weight (42.1 +/- 1.2 kg) and milk yield (2.16 +/- 0.060 kg/goat/day). The experiment was conducted in an incomplete crossover design where one group of four goats was fed a mixed ration of barley grain (BRL), another group of four goats replaced barley grain with orange pulp (OP) and the last group of four goats with soybean hulls (SH). After adaptation to diets, the goats were allocated to individual metabolism cages and intake, faeces, urine and milk were recorded and analysed. Then, gas exchange measurements were recorded by a mobile open-circuit indirect calorimetry system using a head box. Dry matter intake was similar for all three groups (2.03 kg/d, on average). No influence of the diet was observed for energy balance and the efficiency of use of metabolizable energy for milk production was 0.61. The OP and SH diets showed greater (P th Symposium on Energy Metabolism. EAAP. Publ. 11. 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(2010). Effects of stage of lactation and dietary concentrate level on energy utilization by Alpine dairy goats. Journal of Dairy Science, 93(10), 4818-4828. doi:10.3168/jds.2010-3315Bava, L., Rapetti, L., Crovetto, G. M., Tamburini, A., Sandrucci, A., Galassi, G., & Succi, G. (2001). Effects of a Nonforage Diet on Milk Production, Energy, and Nitrogen Metabolism in Dairy Goats throughout Lactation. Journal of Dairy Science, 84(11), 2450-2459. doi:10.3168/jds.s0022-0302(01)74695-4López, M. C., & Fernández, C. (2013). Energy partitioning and substrate oxidation by Murciano-Granadina goats during mid lactation fed soy hulls and corn gluten feed blend as a replacement for corn grain. Journal of Dairy Science, 96(7), 4542-4552. doi:10.3168/jds.2012-6473Palmquist, D. L., & Jenkins, T. C. (1980). Fat in Lactation Rations : Review. Journal of Dairy Science, 63(1), 1-14. doi:10.3168/jds.s0022-0302(80)82881-5Kebreab E, Strathe AB, Dijkstra J, Mills JAN, Reynolds CK, Crompton LA, et al. (2010) Energy and protein interactions and their effects on nitrogen excretion in dairy cows. Pages 417–426 in Symp. on Energy and Protein Metabolism and Nutrition, Parma, Italy.Chilliard, Y., Ferlay, A., Rouel, J., & Lamberet, G. (2003). A Review of Nutritional and Physiological Factors Affecting Goat Milk Lipid Synthesis and Lipolysis. Journal of Dairy Science, 86(5), 1751-1770. doi:10.3168/jds.s0022-0302(03)73761-8Vlaeminck, B., Fievez, V., Cabrita, A. R. J., Fonseca, A. J. M., & Dewhurst, R. J. (2006). Factors affecting odd- and branched-chain fatty acids in milk: A review. Animal Feed Science and Technology, 131(3-4), 389-417. doi:10.1016/j.anifeedsci.2006.06.017Fievez, V., Colman, E., Castro-Montoya, J. M., Stefanov, I., & Vlaeminck, B. (2012). Milk odd- and branched-chain fatty acids as biomarkers of rumen function—An update. Animal Feed Science and Technology, 172(1-2), 51-65. doi:10.1016/j.anifeedsci.2011.12.008Vlaeminck, B., Gervais, R., Rahman, M. M., Gadeyne, F., Gorniak, M., Doreau, M., & Fievez, V. (2015). Postruminal synthesis modifies the odd- and branched-chain fatty acid profile from the duodenum to milk. Journal of Dairy Science, 98(7), 4829-4840. doi:10.3168/jds.2014-9207Jorjong, S., van Knegsel, A. T. M., Verwaeren, J., Bruckmaier, R. M., De Baets, B., Kemp, B., & Fievez, V. (2015). Milk fatty acids as possible biomarkers to diagnose hyperketonemia in early lactation. Journal of Dairy Science, 98(8), 5211-5221. doi:10.3168/jds.2014-8728Ulbricht, T. L. V., & Southgate, D. A. T. (1991). Coronary heart disease: seven dietary factors. The Lancet, 338(8773), 985-992. doi:10.1016/0140-6736(91)91846-mKnapp, J. R., Laur, G. L., Vadas, P. A., Weiss, W. P., & Tricarico, J. M. (2014). Invited review: Enteric methane in dairy cattle production: Quantifying the opportunities and impact of reducing emissions. Journal of Dairy Science, 97(6), 3231-3261. doi:10.3168/jds.2013-7234Angelidaki, I., & Sanders, W. (2004). Assessment of the anaerobic biodegradability of macropollutants. Reviews in Environmental Science and Bio/Technology, 3(2), 117-129. doi:10.1007/s11157-004-2502-3Velthof, G. L., Nelemans, J. A., Oenema, O., & Kuikman, P. J. (2005). Gaseous Nitrogen and Carbon Losses from Pig Manure Derived from Different Diets. Journal of Environmental Quality, 34(2), 698-706. doi:10.2134/jeq2005.0698Jarret, G., Cozannet, P., Martinez, J., & Dourmad, J. Y. (2011). Effect of different quality wheat dried distiller’s grain solubles (DDGS) in pig diets on composition of excreta and methane production from faeces and slurry. Livestock Science, 140(1-3), 275-282. doi:10.1016/j.livsci.2011.04.006Triolo, J. M., Sommer, S. G., Møller, H. B., Weisbjerg, M. R., & Jiang, X. Y. (2011). A new algorithm to characterize biodegradability of biomass during anaerobic digestion: Influence of lignin concentration on methane production potential. Bioresource Technology, 102(20), 9395-9402. doi:10.1016/j.biortech.2011.07.026Klevenhusen, F., Kreuzer, M., & Soliva, C. R. (2010). Enteric and manure-derived methane and nitrogen emissions as well as metabolic energy losses in cows fed balanced diets based on maize, barley or grass hay. animal, 5(03), 450-461. doi:10.1017/s1751731110001795SPEK, J. W., DIJKSTRA, J., VAN DUINKERKEN, G., & BANNINK, A. (2012). A review of factors influencing milk urea concentration and its relationship with urinary urea excretion in lactating dairy cattle. The Journal of Agricultural Science, 151(3), 407-423. doi:10.1017/s0021859612000561Van Knegsel, A. T. M., van den Brand, H., Dijkstra, J., van Straalen, W. M., Heetkamp, M. J. W., Tamminga, S., & Kemp, B. (2007). Dietary Energy Source in Dairy Cows in Early Lactation: Energy Partitioning and Milk Composition. Journal of Dairy Science, 90(3), 1467-1476. doi:10.3168/jds.s0022-0302(07)71632-