Taken to the grave. An archaeozoological approach assessing the role of animals as crematory offerings in first millennium AD Britain.

The crematory funerary rites practiced by those living in parts of mainland Britain during the first millennium AD included burning complete or parts of animals on the pyre. This thesis highlights the potential for archaeozoological analysis of faunal pyre goods using assemblages from the first millennium AD as a dataset. Experimental study and the integration of current research from a number of disciplines is used to suggest that although pyrolysis and cremation practices fragment and distort burnt bone assemblages, careful analysis can reveal a wealth of data leading to the interpretation of various forms of pyre good. The results of the author¿s analysis of material from the sites of Brougham, Cumbria, St. Stephen¿s, Hertfordshire, Castleford, West Yorkshire and Heath Wood, Derbyshire are combined with data from other published cemeteries to suggest a series of chronological and regional continuities in the use of animals but with a distinct change at the start of the Early Medieval period. The results from Brougham are particularly significant as they alter preconceived views on the utilisation of animals in Romano-British funerary practice. Cremation burials in first millennium AD Britain are shown to include the burnt remains of predominantly domestic taxa with occasional wild species. The pyre goods are interpreted as representing food offerings, companions, amulets, gaming items and sacrifices. This thesis demonstrates that cremated animal bone should not be disregarded but rather valued as source of archaeozoological data, and a significant functional tool for interpreting past funerary behaviour and animal utilisation

Understanding Middle Neolithic food and farming in and around the Stonehenge World Heritage Site: an integrated approach

Little synthesis of evidence for Middle Neolithic food and farming in Wiltshire, particularly in and around the Stonehenge World Heritage Site (WHS) has been possible, until now, due to a paucity of assemblages. The excavation of a cluster of five Middle Neolithic pits and an inhumation burial at West Amesbury Farm (WAF) has prompted a review of our understanding of pit sites of this period from the county. Bioarchaeological assemblages are used to investigate evidence for the consumption of animal and plant-based foods, and for agricultural and pastoral farming. For the first time Middle Neolithic zooarchaeological evidence, including strontium isotope data, is considered alongside archaeobotanical data, and radiocarbon dating. The absence of cultivated plants from WAF and contemporary sites in the county is consistent with the hypothesis that the reduction in cereal cultivation and greater reliance of wild plants witnessed in the later part of the Neolithic in southern England began in the Middle Neolithic. The zooarchaeological evidence from the same sites demonstrates that the shift away from cereal cultivation may be concurrent with, rather than earlier than, an increase in the relative proportion of deposited pig bones relative to cattle. Both cattle and pigs deposited in pits at WAF have strontium and sulphur isotope values consistent with the local biosphere, and therefore were potentially raised in the area. Zooarchaeological data from WAF compliments that from human dental calculus and lipid residues in associated Peterborough Ware pottery that local cattle husbandry included exploitation of dairy. It also highlights the presence of consistent food preparation methods between pits as seen through butchery practice. The faunal and archaeobotanical remains from contemporary pit deposits suggest that similar farming and subsistence strategies can be proposed across the county, though with some inter-site variation in deposition. Together these excavated pit sites are likely to represent only a sample of those present in the area. Application of a similar integrated approach to material from other Middle Neolithic pits holds potential for better understanding of food and farming in this previously neglected period

Wild to domestic and back again: the dynamics of fallow deer management in medieval England (c.11th-16th century AD)

This paper presents the results of the first comprehensive scientific study of the fallow deer, a non-native species whose medieval-period introduction to Britain transformed the cultural landscape. It brings together data from traditional zooarchaeological analyses with those derived from new ageing techniques as well as the results of a programme of radiocarbon dating, multi-element isotope studies and genetic analyses. These new data are here integrated with historical and landscape evidence to examine changing patterns of fallow deer translocation and management in medieval England between the 11th and 16th century AD

Middle Neolithic pits and a burial at West Amesbury, Wiltshire

Excavations on the south-eastern slopes of King Barrow Ridge, 1.5 km east of Stonehenge, revealed five pits, a grave and other features of Middle Neolithic date. Analysis of the pit assemblages and the partial inhumation interred in the grave has provided insights into lifeways in this landscape in the late fourth millennium cal BC. Evidence suggests that the area was visited by a pastoralist, mobile community on a semi-regular basis for a significant period, in late autumn or winter. Selected remnants of craft-working and consumption were deposited in pits, before deliberate infilling. These depositions repeatedly memorialised activity on the hillside at a time of contemporary activity elsewhere on King Barrow Ridge and at the future site of Stonehenge. Middle Neolithic pits are present in significant numbers across King Barrow Ridge, and alongside pits in the Durrington area, form one of the densest concentrations of such activity in the region. Long distance mobility is suggested by the possible Irish origins of the inhumation, the first Middle Neolithic individual excavated in the environs of Stonehenge. Whilst of significance for understanding the Middle Neolithic in the WHS and the region, this research also hints at the roots of Late Neolithic monumentalisation of this landscape

The Drosophila melanogaster Genetic Reference Panel

A major challenge of biology is understanding the relationship between molecular genetic variation and variation in quantitative traits, including fitness. This relationship determines our ability to predict phenotypes from genotypes and to understand how evolutionary forces shape variation within and between species. Previous efforts to dissect the genotype–phenotype map were based on incomplete genotypic information. Here, we describe the Drosophila melanogaster Genetic Reference Panel (DGRP), a community resource for analysis of population genomics and quantitative traits. The DGRP consists of fully sequenced inbred lines derived from a natural population. Population genomic analyses reveal reduced polymorphism in centromeric autosomal regions and the X chromosome, evidence for positive and negative selection, and rapid evolution of the X chromosome. Many variants in novel genes, most at low frequency, are associated with quantitative traits and explain a large fraction of the phenotypic variance. The DGRP facilitates genotype–phenotype mapping using the power of Drosophila genetics

Taken to the grave : an archaeozoological approach assessing the role of animals as crematory offerings in first millennium AD Britain

The crematory funerary rites practiced by those living in parts of mainland Britain during the first millennium AD included burning complete or parts of animals on the pyre. This thesis highlights the potential for archaeozoological analysis of faunal pyre goods using assemblages from the first millennium AD as a dataset. Experimental study and the integration of current research from a number of disciplines is used to suggest that although pyrolysis and cremation practices fragment and distort burnt bone assemblages, careful analysis can reveal a wealth of data leading to the interpretation of various forms of pyre good. The results of the author's analysis of material from the sites of Brougham, Cumbria, St. Stephen's, Hertfordshire, Castleford, West Yorkshire and Heath Wood, Derbyshire are combined with data from other published cemeteries to suggest a series of chronological and regional continuities in the use of animals but with a distinct change at the start of the Early Medieval period. The results from Brougham are particularly significant as they alter preconceived views on the utilisation of animals in Romano-British funerary practice. Cremation burials in first millennium AD Britain are shown to include the burnt remains of predominantly domestic taxa with occasional wild species. The pyre goods are interpreted as representing food offerings, companions, amulets, gaming items and sacrifices. This thesis demonstrates that cremated animal bone should not be disregarded but rather valued as source of archaeozoological data, and a significant functional tool for interpreting past funerary behaviour and animal utilisation.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

NMR Metabolomics Protocols for Drug Discovery

Drug discovery is an extremely difficult and challenging endeavor with a very high failure rate. The task of identifying a drug that is safe, selective and effective is a daunting proposition because disease biology is complex and highly variable across patients. Metabolomics enables the discovery of disease biomarkers, which provides insights into the molecular and metabolic basis of disease and may be used to assess treatment prognosis and outcome. In this regard, metabolomics has evolved to become an important component of the drug discovery process to resolve efficacy and toxicity issues, and as a tool for precision medicine. A detailed description of an experimental protocol is presented that outlines the application of NMR metabolomics to the drug discovery pipeline. This includes: (1) target identification by understanding the metabolic dysregulation in diseases, (2) predicting the mechanism of action of newly discovered or existing drug therapies, (3) and using metabolomics to screen a chemical lead to assess biological activity. Unlike other OMICS approaches, the metabolome is “fragile”, and may be negatively impacted by improper sample collection, storage and extraction procedures. Similarly, biologically-irrelevant conclusions may result from incorrect data collection, pre-processing or processing procedures, or the erroneous use of univariate and multivariate statistical methods. These critical concerns are also addressed in the protocol