62 research outputs found
Agent-based spatiotemporal simulation of biomolecular systems within the open source MASON framework
Agent-based modelling is being used to represent biological systems with increasing frequency and success. This paper presents the implementation of a new tool for biomolecular reaction modelling in the open source Multiagent Simulator of Neighborhoods framework. The rationale behind this new tool is the necessity to describe interactions at the molecular level to be able to grasp emergent and meaningful biological behaviour. We are particularly interested in characterising and quantifying the various effects that facilitate biocatalysis. Enzymes may display high specificity for their substrates and this information is crucial to the engineering and optimisation of bioprocesses. Simulation results demonstrate that molecule distributions, reaction rate parameters, and structural parameters can be adjusted separately in the simulation allowing a comprehensive study of individual effects in the context of realistic cell environments. While higher percentage of collisions with occurrence of reaction increases the affinity of the enzyme to the substrate, a faster reaction (i.e., turnover number) leads to a smaller number of time steps. Slower diffusion rates and molecular crowding (physical hurdles) decrease the collision rate of reactants, hence reducing the reaction rate, as expected. Also, the random distribution of molecules affects the results significantly.The authors thank the Agrupamento INBIOMED from DXPCTSUG-FEDER unha maneira de facer Europa (2012/273). The research leading to these results has received funding from the European Union's Seventh Framework Programme FP7/REGPOT-2012-2013.1 under Grant Agreement no. 316265 (BIOCAPS) and the [14VI05] Contract-Programme from the University of Vigo. This document reflects only the authors' views and the European Union is not liable for any use that may be made of the information contained herein
WhichGenes: a web-based tool for gathering, building, storing and exporting gene sets with application in gene set enrichment analysis
WhichGenes is a web-based interactive gene set building tool offering a very simple interface to extract always-updated gene lists from multiple databases and unstructured biological data sources. While the user can specify new gene sets of interest by following a simple four-step wizard, the tool is able to run several queries in parallel. Every time a new set is generated, it is automatically added to the private gene-set cart and the user is notified by an e-mail containing a direct link to the new set stored in the server. WhichGenes provides functionalities to edit, delete and rename existing sets as well as the capability of generating new ones by combining previous existing sets (intersection, union and difference operators). The user can export his sets configuring the output format and selecting among multiple gene identifiers. In addition to the user-friendly environment, WhichGenes allows programmers to access its functionalities in a programmatic way through a Representational State Transfer web service. WhichGenes front-end is freely available at http://www.whichgenes.org/, WhichGenes API is accessible at http://www.whichgenes.org/api/
Proposing to use artificial neural Networks for NoSQL attack detection
[EN] Relationships databases have enjoyed a certain boom in software
worlds until now. These days, with the rise of modern applications, unstructured
data production, traditional databases do not completely meet the needs of all
systems. Regarding these issues, NOSQL databases have been developed and
are a good alternative. But security aspects stay behind. Injection attacks are the
most serious class of web attacks that are not taken seriously in NoSQL.
This paper presents a Neural Network model approach for NoSQL injection.
This method attempts to use the best and most effective features to identify an
injection. The features used are divided into two categories, the first one based
on the content of the request, and the second one independent of the request
meta parameters. In order to detect attack payloads features, we work on
character level analysis to obtain malicious rate of user inputs. The results
demonstrate that our model has detected more attack payloads compare with
models that work black list approach in keyword level
Predicting Specificities Under the Non-self Gametophytic Self-Incompatibility Recognition Model
Non-self gametophytic self-incompatibility (GSI) recognition system is characterized by
the presence of multiple F-box genes tandemly located in the S-locus, that regulate
pollen specificity. This reproductive barrier is present in Solanaceae, Plantaginacea and
Maleae (Rosaceae), but only in Petunia functional assays have been performed to get
insight on how this recognition mechanism works. In this system, each of the encoded
S-pollen proteins (called SLFs in Solanaceae and Plantaginaceae /SFBBs in Maleae)
recognizes and interacts with a sub-set of non-self S-pistil proteins, called S-RNases,
mediating their ubiquitination and degradation. In Petunia there are 17 SLF genes
per S-haplotype, making impossible to determine experimentally each SLF specificity.
Moreover, domain –swapping experiments are unlikely to be performed in large scale to
determine S-pollen and S-pistil specificities. Phylogenetic analyses of the Petunia SLFs
and those from two Solanum genomes, suggest that diversification of SLFs predate the
two genera separation. Here we first identify putative SLF genes from nine Solanum
and 10 Nicotiana genomes to determine how many gene lineages are present in the
three genera, and the rate of origin of new SLF gene lineages. The use of multiple
genomes per genera precludes the effect of incompleteness of the genome at the
S-locus. The similar number of gene lineages in the three genera implies a comparable
effective population size for these species, and number of specificities. The rate of origin
of new specificities is one per 10 million years. Moreover, here we determine the amino
acids positions under positive selection, those involved in SLF specificity recognition,
using 10 Petunia S-haplotypes with more than 11 SLF genes. These 16 amino acid
positions account for the differences of self-incompatible (SI) behavior described in the
literature. When SLF and S-RNase proteins are divided according to the SI behavior, and the positively selected amino acids classified according to hydrophobicity, charge, polarity and size, we identified fixed differences between SI groups. According to the in silico 3D structure of the two proteins these amino acid positions interact. Therefore, this methodology can be used to infer SLF/S-RNase specificity recognition.This work was financed by the project Norte-01-0145-FEDER-000008-Porto Neurosciences and Neurologic Disease Research Initiative at I3S, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). SR is supported by a post-doctoral fellowship under this project. HL-F is supported by a post-doctoral fellowship from Xunta de Galicia (ED481B 2016/068-0). SING group acknowledges Consellería de Educación, Universidades e Formación Profesional (Xunta de Galicia) for the ED431C2018/55-GRC grant and CITI (Centro de Investigación, Transferencia e Innovación) from University of Vigo for hosting its IT infrastructure
ATXN1 N-terminal region explains the binding differences of wild-type and expanded forms
BACKGROUND:
Wild-type (wt) polyglutamine (polyQ) regions are implicated in stabilization of protein-protein interactions (PPI). Pathological polyQ expansion, such as that in human Ataxin-1 (ATXN1), that causes spinocerebellar ataxia type 1 (SCA1), results in abnormal PPI. For ATXN1 a larger number of interactors has been reported for the expanded (82Q) than the wt (29Q) protein.
METHODS:
To understand how the expanded polyQ affects PPI, protein structures were predicted for wt and expanded ATXN1, as well as, for 71 ATXN1 interactors. Then, the binding surfaces of wt and expanded ATXN1 with the reported interactors were inferred.
RESULTS:
Our data supports that the polyQ expansion alters the ATXN1 conformation and that it enhances the strength of interaction with ATXN1 partners. For both ATXN1 variants, the number of residues at the predicted binding interface are greater after the polyQ, mainly due to the AXH domain. Moreover, the difference in the interaction strength of the ATXN1 variants was due to an increase in the number of interactions at the N-terminal region, before the polyQ, for the expanded form.
CONCLUSIONS:
There are three regions at the AXH domain that are essential for ATXN1 PPI. The N-terminal region is responsible for the strength of the PPI with the ATXN1 variants. How the predicted motifs in this region affect PPI is discussed, in the context of ATXN1 post-transcriptional modifications.This work was financed by the project Norte-01-0145-FEDER-000008 -Porto
Neurosciences and Neurologic Disease Research Initiative at I3S, supported
by Norte Portugal Regional Operational Programme (NORTE 2020), under the
PORTUGAL 2020 Partnership Agreement, through the European Regional Development
Fund (FEDER). Sara Rocha is supported by a post-doctoral fellowship
under this project. Hugo López-Fernández is supported by a postdoctoral
fellowship from Xunta de Galicia (ED481B 2016/068–0). SING group
thanks Consellería de Educación, Universidades e Formación Profesional
(Xunta de Galicia) for the ED431C2018/55-GRC grant and CITI (Centro de
Investigación, Transferencia e Innovación) from University of Vigo for hosting
its IT infrastructure. The funding bodies played no role in the design of the
study and collection, analysis, and interpretation of data and in writing the
manuscript
Mass-Up and Decision Peptide-Driven: two open-source applications for MALDI-TOF MS data analysis and protein quantification
Poster presentation at EUBIC Winter School 2017 (10th - 13th January 2017, Sporthotel Semmering, Austria)
Multiple independent L-gulonolactone oxidase (GULO) gene losses and vitamin C synthesis reacquisition events in non-Deuterostomian animal species
Background: L-ascorbate (Vitamin C) is an important antioxidant and co-factor in eukaryotic cells, and in mammals it is indispensable for brain development and cognitive function. Vertebrates usually become L-ascorbate auxothrophs when the last enzyme of the synthetic pathway, an L-gulonolactone oxidase (GULO), is lost. Since Protostomes were until recently thought not to have a GULO gene, they were considered to be auxothrophs for Vitamin C. Results: By performing phylogenetic analyses with tens of non-Bilateria and Protostomian genomes, it is shown, that a GULO gene is present in the non-Bilateria Placozoa, Myxozoa (here reported for the first time) and Anthozoa groups, and in Protostomians, in the Araneae family, the Gastropoda class, the Acari subclass (here reported for the first time), and the Priapulida, Annelida (here reported for the first time) and Brachiopoda phyla lineages. GULO is an old gene that predates the separation of Animals and Fungi, although it could be much older. We also show that within Protostomes, GULO has been lost multiple times in large taxonomic groups, namely the Pancrustacea, Nematoda, Platyhelminthes and Bivalvia groups, a pattern similar to that reported for Vertebrate species. Nevertheless, we show that Drosophila melanogaster seems to be capable of synthesizing L-ascorbate, likely through an alternative pathway, as recently reported for Caenorhabditis elegans. Conclusions: Non-Bilaterian and Protostomians seem to be able to synthesize Vitamin C either through the conventional animal pathway or an alternative pathway, but in this animal group, not being able to synthesize L-ascorbate seems to be the exception rather than the rule.This work is financed by the project Norte-01-0145-FEDER-000008 - Porto Neurosciences and Neurologic Disease Research Initiative at I3S, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). S. F. Henriques is supported by a post-doctoral fellowship also funded by the above mentioned project. SING group is supported by the Consellería de Educación, Universidades e Formación Pro-fesional (Xunta de Galicia) by the ED431C2018/55-GRC grant. H. López-Fer-nández is supported by a post-doctoral fellowship from Xunta de Galicia (ED481B 2016/068–0). The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript
A spam filtering mult-iobjective optimization study covering parsimony maximization and three-way classification
The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.Classifier performance optimization in machine learning can be stated as a multi-objective optimization problem. In this context, recent works have shown the utility of simple evolutionary multi-objective algorithms (NSGA-II, SPEA2) to conveniently optimize the global performance of different anti-spam filters. The present work extends existing contributions in the spam filtering domain by using three novel indicator-based (SMS-EMOA, CH-EMOA) and decomposition-based (MOEA/D) evolutionary multi-objective algorithms. The proposed approaches are used to optimize the performance of a heterogeneous ensemble of classifiers into two different but complementary scenarios: parsimony maximization and e-mail classification under low confidence level. Experimental results using a publicly available standard corpus allowed us to identify interesting conclusions regarding both the utility of rule-based classification filters and the appropriateness of a three-way classification system in the spam filtering domain
PileLineGUI: a desktop environment for handling genome position files in next-generation sequencing studies
Next-generation sequencing (NGS) technologies are making sequence data available on an unprecedented scale. In this context, new catalogs of Single Nucleotide Polymorphism and mutations generated by resequencing studies are usually stored in genome position files (e.g. Variant Call Format, SAMTools pileup, BED, GFF) comprising of large lists of genomic positions, which are difficult to handle by researchers. Here, we present PileLineGUI, a novel desktop application primarily designed for manipulating, browsing and analysing genome position files (GPF), with specific support to somatic mutation finding studies. The developed tool also integrates a new genome browser module specially designed for inspecting GPFs. PileLineGUI is free, multiplatform and designed to be intuitively used by biomedical researchers. PileLineGUI is available at: http://sing.ei.uvigo.es/pileline/pilelinegui.html
Learning Object Repositories with Federated Searcher over the Cloud
The education sector is a significant generator, consumer and depository for educational content. Educators and Learners have access to technologies that allow them to obtain information ubiquitously on demand. The problems arising from the integration of educational content are usually caused by the vast amount of educational content distributed among several repositories. This work presents a proposal for an architecture based on a cloud computing paradigm that will permit the evolution of current learning resource repositories by means of cloud computing paradigm and the integration of federated search system
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