Site-fidelity and movement patterns of bottlenose dolphins (<i>Tursiops truncatus</i>) in central Argentina: essential information for effective conservation

The effectiveness of conservation measures such as marine protected areas (MPAs) for the conservation of cetaceans is determined by how well their home range or critical habitat is covered. The present study seeks to provide information on the site-fidelity and movement patterns of individual bottlenose dolphins (Tursiops truncatus) in central Argentina. Between 2007 and 2013, photo-identification data of bottlenose dolphins were collected in four study sites some 90-200km apart from each other along the central Argentinean coast. Results show long-term site-fidelity (over 5years) in one of the study areas. Re-sighting rates further suggest the existence of different sub-populations of bottlenose dolphins, but also confirm some connectivity (with movements over 200-290km) and thus potential for gene flow within the region. Considering the population declines of bottlenose dolphins in Argentina, information on site-fidelity and movement patterns will be of value to improve the effectiveness of existing MPAs for the conservation of the species as well as prioritizing areas for increased research

Activation of the E-cadherin/catenin complex in human MCF-7 breast cancer cells by all-trans-retinoic acid.

All-trans-retinoic acid (RA), like insulin-like growth factor I (IGF-I) and tamoxifen, inhibit invasion of human MCF-7/6 mammary cancer cells in vitro. For tamoxifen and for IGF-I, activation of the invasion-suppressor function of the E-cadherin/catenin complex was shown to be the most probable mechanism of the anti-invasive action. We did a series of experiments to determine whether the anti-invasive effect of RA also implicated the invasion-suppressor E-cadherin/catenin complex. Human MCF-7/6 mammary and HCT-8/R1 colon cancer cells, both with a dysfunctional E-cadherin/catenin complex, were treated with RA and the function of the complex was evaluated through Ca(2+)-dependent fast aggregation. Fast aggregation of both MCF-7/6 and HCT-8/R1 cells was induced by 1 microM RA. This effect was abolished by antibodies against E-cadherin. RA-induced fast aggregation was not sensitive to cycloheximide, tyrosine kinase inhibitors or antibodies against IGF-I or against the IGF-I receptor. RA did not stimulate IGF-I receptor phosphorylation or alter the E-cadherin/catenin complex, as evidenced by immunoprecipitation. RA up-regulates the function of the invasion-suppressor complex E-cadherin/catenin. Its action mechanism is different from that of IGF-I. RA may act as an anti-invasive agent with unique mechanisms of action

MX100, a new Escherichia coli tester strain for use in genotoxicity studies

The development of a new Escherichia coli tester strain for use in metabolic and mechanistic studies of genotoxins, strain MR2101/pKR11, has recently been reported. This strain, a derivative of the E.coli K12 laboratory strain AB1157, has sensitivity towards the detection of base-substitution mutagenesis, monitored by the reversion of arginine auxotrophy [argE3, (ochre)]. Besides arginine, MR2101/pKR11 is auxotrophic for histidine (hisG4), leucine (leuB6), proline (ΔproA) and threonine (thr-1). MX100 was developed to overcome the auxotrophy for four amino acids of MR2101/pKR11 which are non-essential for the mutagenic responsiveness of the strain. We restored the biosynthesis for these four amino acids in MR2101/pKR11, resulting in strain MX100. This strain showed an almost 2-fold increase in mutagenic activity relative to MR2101/pKR11 with a set of diagnostic mutagens (aflatoxin B1, benzo[α]pyrene, 4-nitroquinoline-1-oxide, 2,7-dimethyl-benz[a]anthracene and others) and was further characterized with other types of mutagens in which it showed sensitivity towards the detection of oxidative (H2O2, t-butyl-hydroperoxide, cumene-hydroperoxide, KO2) and carbonyl mutagens (methylglyoxal, malondialdehyde). As MX100 seems to have the right characteristics of a versatile genotoxicity tester strain and due to the extensive genetic and physiological knowledge of E.coli K12 in general and AB1157 in particular, we propose that MX100 could serve as mother strain for the development of specialized tester strains, of interest in studies of metabolism and/or mechanism of action of genotoxic carcinogens.publishersversionpublishe

Doppler Boosting, Superluminal Motion, and the Kinematics of AGN Jets

We discuss results from a decade long program to study the fine-scale structure and the kinematics of relativistic AGN jets with the aim of better understanding the acceleration and collimation of the relativistic plasma forming AGN jets. From the observed distribution of brightness temperature, apparent velocity, flux density, time variability, and apparent luminosity, the intrinsic properties of the jets including Lorentz factor, luminosity, orientation, and brightness temperature are discussed. Special attention is given to the jet in M87, which has been studied over a wide range of wavelengths and which, due to its proximity, is observed with excellent spatial resolution. Most radio jets appear quite linear, but we also observe curved non-linear jets and non-radial motions. Sometimes, different features in a given jet appear to follow the same curved path but there is evidence for ballistic trajectories as well. The data are best fit with a distribution of Lorentz factors extending up to gamma ~30 and intrinsic luminosity up to ~10^26 W/Hz. In general, gamma-ray quasars may have somewhat larger Lorentz factors than non gamma-ray quasars. Initially the observed brightness temperature near the base of the jet extend up to ~5x10^13 K which is well in excess of the inverse Compton limit and corresponds to a large excess of particle energy over magnetic energy. However, more typically, the observed brightness temperatures are ~2x10^11 K, i.e., closer to equipartition.Comment: 10 pages, 12 color figures; proceedings of the 5th Stromlo Symposium: Disks, Winds, and Jets - from Planets to Quasars; accepted in Astrophysics & Space Scienc

MOJAVE: monitoring of jets in active galactic nuclei with VLBA experiments. V. Multi-epoch VLBA images

We present images from a long-term program (MOJAVE: Monitoring of Jets in active galactic nuclei (AGNs) with VLBA Experiments) to survey the structure and evolution of parsec-scale jet phenomena associated with bright radio-loud active galaxies in the northern sky. The observations consist of 2424 15 GHz Very Long Baseline Array (VLBA) images of a complete flux-density-limited sample of 135 AGNs above declination –20°, spanning the period 1994 August to 2007 September. These data were acquired as part of the MOJAVE and 2 cm Survey programs, and from the VLBA archive. The sample-selection criteria are based on multi-epoch parsec-scale (VLBA) flux density, and heavily favor highly variable and compact blazars. The sample includes nearly all the most prominent blazars in the northern sky, and is well suited for statistical analysis and comparison with studies at other wavelengths. Our multi-epoch and stacked-epoch images show 94% of the sample to have apparent one-sided jet morphologies, most likely due to the effects of relativistic beaming. Of the remaining sources, five have two-sided parsec-scale jets, and three are effectively unresolved by the VLBA at 15 GHz, with essentially all of the flux density contained within a few tenths of a milliarcsecond

TransCom N2O model inter-comparison - Part 2:Atmospheric inversion estimates of N2O emissions

This study examines N2O emission estimates from five different atmospheric inversion frameworks based on chemistry transport models (CTMs). The five frameworks differ in the choice of CTM, meteorological data, prior uncertainties and inversion method but use the same prior emissions and observation data set. The posterior modelled atmospheric N2O mole fractions are compared to observations to assess the performance of the inversions and to help diagnose problems in the modelled transport. Additionally, the mean emissions for 2006 to 2008 are compared in terms of the spatial distribution and seasonality. Overall, there is a good agreement among the inversions for the mean global total emission, which ranges from 16.1 to 18.7 TgN yr(-1) and is consistent with previous estimates. Ocean emissions represent between 31 and 38% of the global total compared to widely varying previous estimates of 24 to 38%. Emissions from the northern mid- to high latitudes are likely to be more important, with a consistent shift in emissions from the tropics and subtropics to the mid- to high latitudes in the Northern Hemisphere; the emission ratio for 0-30A degrees N to 30-90A degrees N ranges from 1.5 to 1.9 compared with 2.9 to 3.0 in previous estimates. The largest discrepancies across inversions are seen for the regions of South and East Asia and for tropical and South America owing to the poor observational constraint for these areas and to considerable differences in the modelled transport, especially inter-hemispheric exchange rates and tropical convective mixing. Estimates of the seasonal cycle in N2O emissions are also sensitive to errors in modelled stratosphere-to-troposphere transport in the tropics and southern extratropics. Overall, the results show a convergence in the global and regional emissions compared to previous independent studies

Climate smart agriculture (CSA)

Climate change fundamentally shifts the agricultural development agenda. Changing temperature and precipitation, sea level rise, and the rising frequency of extreme climate events will significantly reduce global food production in this century unless action is taken. Major investments, private and public, will be needed.! Adapting agriculture to climate change is necessary to achieve food security, and agricultural mitigation can also reduce atmospheric greenhouse gas concentrations and slow climate change itself. There are many drivers of change affecting agricultural sectors around the world, including population growth, changes in consumer demand and market integration. Climate Smart Agriculture is an integrated approach to achieve food security in the face of climate change, while also mitigating climate change and contribute to other development goals