246 research outputs found
Effects of cortical FoxP1 knockdowns on learned song preference in female zebra finches
The search for molecular underpinnings of human vocal communication has focused on genes encoding forkhead-box transcription factors, as rare disruptions of FOXP1, FOXP2, and FOXP4 have been linked to disorders involving speech and language deficits. In male songbirds, an animal model for vocal learning, experimentally altered expression levels of these transcription factors impair song production learning. The relative contributions of auditory processing, motor function or auditory-motor integration to the deficits observed after different FoxP manipulations in songbirds are unknown. To examine the potential effects on auditory learning and development, we focused on female zebra finches (Taeniopygia guttata) that do not sing but develop song memories, which can be assayed in operant preference tests. We tested whether the relatively high levels of FoxP1 expression in forebrain areas implicated in female song preference learning are crucial for the development and/or maintenance of this behavior. Juvenile and adult female zebra finches received FoxP1 knockdowns targeted to HVC (proper name) or to the caudomedial mesopallium (CMM). Irrespective of target site and whether the knockdown took place before (juveniles) or after (adults) the sensitive phase for song memorization, all groups preferred their tutor’s song. However, adult females with FoxP1 knockdowns targeted at HVC showed weaker motivation to hear song and weaker song preferences than sham-treated controls, while no such differences were observed after knockdowns in CMM or in juveniles. In summary, FoxP1 knockdowns in the cortical song nucleus HVC were not associated with impaired tutor song memory but reduced motivation to actively request tutor songs.NWOAnimal science
Expanding the set of rhodococcal Baeyer–Villiger monooxygenases by high-throughput cloning, expression and substrate screening
To expand the available set of Baeyer–Villiger monooxygenases (BVMOs), we have created expression constructs for producing 22 Type I BVMOs that are present in the genome of Rhodococcus jostii RHA1. Each BVMO has been probed with a large panel of potential substrates. Except for testing their substrate acceptance, also the enantioselectivity of some selected BVMOs was studied. The results provide insight into the biocatalytic potential of this collection of BVMOs and expand the biocatalytic repertoire known for BVMOs. This study also sheds light on the catalytic capacity of this large set of BVMOs that is present in this specific actinomycete. Furthermore, a comparative sequence analysis revealed a new BVMO-typifying sequence motif. This motif represents a useful tool for effective future genome mining efforts.
The evolutionary state of Miras with changing pulsation periods
Context: Miras are long-period variables thought to be in the asymptotic
giant branch (AGB) phase of evolution. In about one percent of known Miras, the
pulsation period is changing. It has been speculated that this changing period
is the consequence of a recent thermal pulse in these stars. Aims: We aim to
clarify the evolutionary state of these stars, and to determine in particular
whether or not they are in the thermally-pulsing (TP-)AGB phase. Methods: One
important piece of information that has been neglected so far when determining
the evolutionary state is the presence of the radio-active s-process element
technetium (Tc). We obtained high-resolution, high signal-to-noise-ratio
optical spectra of a dozen prominent Mira variables with changing pulsation
period to search for this indicator of TPs and dredge-up. We also use the
spectra to measure lithium (Li) abundances. Furthermore, we establish the
evolutionary states of our sample stars by means of their present-day periods
and luminosities. Results: Among the twelve sample stars observed in this
programme, five were found to show absorption lines of Tc. BH Cru is found to
be a carbon-star, its period increase in the past decades possibly having
stopped by now. We report a possible switch in the pulsation mode of T UMi from
Mira-like to semi-regular variability in the past two years. R Nor, on the
other hand, is probably a fairly massive AGB star, which could be true for all
meandering Miras. Finally, we assign RU Vul to the metal-poor thick disk with
properties very similar to the short-period, metal-poor Miras. Conclusions: We
conclude that there is no clear correlation between period change class and Tc
presence. The stars that are most likely to have experienced a recent TP are BH
Cru and R Hya, although their rates of period change are quite different.Comment: 11 pages, 7 figures, 2 tables; accepted for publication in A&
Cold Dust in Three Massive Evolved Stars in the LMC
Massive evolved stars can produce large amounts of dust, and far-infrared
(IR) data are essential for determining the contribution of cold dust to the
total dust mass. Using Herschel, we search for cold dust in three very dusty
massive evolved stars in the Large Magellanic Cloud: R71 is a Luminous Blue
Variable, HD36402 is a Wolf-Rayet triple system, and IRAS05280-6910 is a red
supergiant. We model the spectral energy distributions using radiative transfer
codes and find that these three stars have mass-loss rates up to 10^-3 solar
masses/year, suggesting that high-mass stars are important contributors to the
life-cycle of dust. We found far-IR excesses in two objects, but these excesses
appear to be associated with ISM and star-forming regions. Cold dust (T < 100
K) may thus not be an important contributor to the dust masses of evolved
stars.Comment: accepted to A&A as part of the Herschel first results special issu
Weissella confusa Infection in Primate (Cercopithecus mona)
We describe systemic infection by Weissella confusa in a mona monkey (Cercopithecus mona) on the basis of microbiologic, molecular genetic, and histologic data. The same strain of W. confusa, as determined by pulsed-field gel electrophoresis, was isolated in pure culture from the primate’s brain, liver, spleen, and intestine. Histologic lesions showed inflammatory infiltrates mainly composed of neutrophils, indicating an acute septicemic process
The ALMA detection of CO rotational line emission in AGB stars in the Large Magellanic Cloud
Context. Low- and intermediate-mass stars lose most of their stellar mass at the end of their lives on the asymptotic giant branch (AGB). Determining gas and dust mass-loss rates (MLRs) is important in quantifying the contribution of evolved stars to the enrichment of the interstellar medium.
Aims: This study attempts to spectrally resolve CO thermal line emission in a small sample of AGB stars in the Large Magellanic Cloud (LMC).
Methods: The Atacama Large Millimeter Array was used to observe two OH/IR stars and four carbon stars in the LMC in the CO J = 2-1 line.
Results: We present the first measurement of expansion velocities in extragalactic carbon stars. All four C stars are detected and wind expansion velocities and stellar velocities are directly measured. Mass-loss rates are derived from modelling the spectral energy distribution and Spitzer/IRS spectrum with the DUSTY code. The derived gas-to-dust ratios allow the predicted velocities to agree with the observed gas-to-dust ratios. The expansion velocities and MLRs are compared to a Galactic sample of well-studied relatively low MLRs stars supplemented with extreme C stars with properties that are more similar to the LMC targets. Gas MLRs derived from a simple formula are significantly smaller than those derived from dust modelling, indicating an order of magnitude underestimate of the estimated CO abundance, time-variable mass loss, or that the CO intensities in LMC stars are lower than predicted by the formula derived for Galactic objects. This could be related to a stronger interstellar radiation field in the LMC.
Conclusions: Although the LMC sample is small and the comparison to Galactic stars is non-trivial because of uncertainties in their distances (hence luminosities), it appears that for C stars the wind expansion velocities in the LMC are lower than in the solar neighbourhood, while the MLRs appear to be similar. This is in agreement with dynamical dust-driven wind models
Sexual Dimorphism of the Zebra Finch Syrinx Indicates Adaptation for High Fundamental Frequencies in Males
In many songbirds the larger vocal repertoire of males is associated with sexual dimorphism of the vocal control centers and muscles of the vocal organ, the syrinx. However, it is largely unknown how these differences are translated into different acoustic behavior.Here we show that the sound generating structures of the syrinx, the labia and the associated cartilaginous framework, also display sexual dimorphism. One of the bronchial half rings that position and tense the labia is larger in males, and the size and shape of the labia differ between males and females. The functional consequences of these differences were explored by denervating syringeal muscles. After denervation, both sexes produced equally low fundamental frequencies, but the driving pressure generally increased and was higher in males. Denervation strongly affected the relationship between driving pressure and fundamental frequency.The syringeal modifications in the male syrinx, in concert with dimorphisms in neural control and muscle mass, are most likely the foundation for the potential to generate an enhanced frequency range. Sexually dimorphic vocal behavior therefore arises from finely tuned modifications at every level of the motor cascade. This sexual dimorphism in frequency control illustrates a significant evolutionary step towards increased vocal complexity in birds
Long-period variables in NGC147 and NGC185
Previous studies on the stellar content of the two nearby dwarf galaxies
NGC147 and NGC185 reveal a rich population of late-type giants in both systems,
including a large number of carbon-rich objects. These stars are known to show
pronounced photometric variability, which can be used for a more detailed
characterisation of these highly evolved stars. Owing to their well-studied
parameters, these Local Group members are ideal candidates for comparative
studies. Trough photometric monitoring, we attempt to provide a catalogue of
long-period variables (LPVs), including Mira variables, semi-regular variables,
and even irregular variables in NGC 147 and NGC185. We investigate the light
variations and compare the characteristics of these two LPV populations with
the results found for other galaxies such as the LMC. We carried out
time-series photometry in the i-band of the two target galaxies with the Nordic
Optical Telescope (NOT), covering a time span of \approx2.5 years. These data
were then combined with single-epoch K-band photometry, also obtained with the
NOT. Narrow-band photometry data from the literature was used to distinguish
between O-rich and C-rich stars. We report the detection of 513 LPVs in NGC185
and 213 LPVs in NGC147, showing i-amplitudes of up to \approx 2mag and periods
ranging between 90 and 800 days. The period-luminosity diagram for each of our
target galaxies exhibits a well populated sequence of fundamental mode
pulsators. The resulting period-luminosity relations we obtained are compared
to relations from the literature. We discuss the universality of those
relations because of which, as a side result, a correction of the distance
modulus of NGC185 may be necessary. Only one of our two galaxies, namely
NGC185, has a significant fraction of possibly first overtone pulsators. An
interpretation of this finding in terms of differences in the star-formation
histories is suggested.Comment: 10 pages (excluding online tables) and 9 figure
Atypical birdsong and artificial languages provide insights into how communication systems are shaped by learning, use and transmission
In this article, I argue that a comparative approach focusing on the cognitive capacities and behavioral mechanisms that underlie vocal learning in songbirds and humans can provide valuable insights into the evolutionary origins of language. The experimental approaches I discuss use abnormal song and atypical linguistic input to study the processes of individual learning, social interaction, and cultural transmission. Atypical input places increased learning and communicative pressure on learners, so exploring how they respond to this type of input provides a particularly clear picture of the biases and constraints at work during learning and use. Furthermore, simulating the cultural transmission of these unnatural communication systems in the laboratory informs us about how learning and social biases influence the structure of communication systems in the long run. Findings based on these methods suggest fundamental similarities in the basic social–cognitive mechanisms underlying vocal learning in birds and humans, and continuing research promises insights into the uniquely human mechanisms and into how human cognition and social behavior interact, and ultimately impact on the evolution of language
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