A Case Study in Matching Test and Proof Coverage

AbstractThis paper studies the complementarity of test and deductive proof processes for Java programs specified in JML (Java Modeling Language). The proof of a program may be long and difficult, especially when automatic provers give up. When a theorem is not automatically proved, there are two possibilities: either the theorem is correct and there are not enough pieces of information to deal with the proof, or the theorem is incorrect. In order to discriminate between those two alternatives, testing techniques can be used. Here, we present experiments around the use of the JACK tool to prove Java programs annotated with JML assertions. When JACK fails to decide proof obligations, we use a combinatorial testing tool, TOBIAS, to produce large test suites that exercise the unproved program parts. The key issue is to establish the relevance of the test suite with respect to the unproved proof obligations. Therefore, we use code coverage techniques: our approach takes advantage of the statement orientation of the JACK tool to compare the statements involved in the unproved proof obligations and the statements covered by the test suite. Finally, we ensure our confidence within the test suites, by evaluating them on mutant program killing exercises. These techniques have been put into practice and are illustrated by a simple case study

Vegetation and climate changes in the forest of Campinas, São Paulo State, Brazil, during the last 25,000 cal yr BP

A paleoenvironmental reconstruction was performed in a Riparian Forest near Campinas to improve knowledge of paleoclimate and paleoenvironment in the State of São Paulo, Brazil. A sediment core of 182 cm depth was collected in a swamp located within a Cerrado/Seasonal Semi-deciduous ecotone forest. Te chronological frame is given by eight radiocarbon dating methods. Pollen and stable isotope analyses (d 13C and d 15N) were performed all along the core. Modern pollen rain is based on fve surface samples collected along the Riparian Forest. Results show a sequence of changes in vegetation and climate between 25 and 13 cal kyr before present (BP), and from 4 cal kyr BP to the present time, with a hiatus between 11 and 4 kyr cal BP. Drier climatic conditions characterized the late Pleistocene and early Holocene, although they had moisture peaks able to maintain an open forest. Te Riparian Forest became fully installed from 4 cal kyr BP onward. Our results are in agreement with other regional studies and contribute to build a regional frame for past climatic conditions at the latitude of São Paulo.493CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informaçãoSem informação2010/16507-

Infrared emission spectrum and potentials of 0u+0_u^+ and 0g+0_g^+ states of Xe2_2 excimers produced by electron impact

We present an investigation of the Xe$_{2}$ excimer emission spectrum observed in the near infrared range about 7800 cm$^{-1}$ in pure Xe gas and in an Ar (90%) --Xe (10%) mixture and obtained by exciting the gas with energetic electrons. The Franck--Condon simulation of the spectrum shape suggests that emission stems from a bound--free molecular transition never studied before. The states involved are assigned as the bound $(3)0_{u}^{+}$ state with $6p [1/2]_{0}$ atomic limit and the dissociative $(1)0_{g}^{+}$ state with $6s [3/2]_{1}$ limit. Comparison with the spectrum simulated by using theoretical potentials shows that the dissociative one does not reproduce correctly the spectrum features.Comment: 4 pages, 3 figures, submitted to Phys. Rev. Let

Countering elevated CO2 induced Fe and Zn reduction in Arabidopsis seeds

Growth at increased concentrations of CO2 induces a reduction in seed zinc (Zn) and iron (Fe). Using Arabidopsis thaliana, we investigated whether this could be mitigated by reducing the elevated CO2-induced decrease in transpiration. We used an infrared imaging-based screen to isolate mutants in At1g08080 that encodes ALPHA CARBONIC ANHYDRASE 7 (ACA7). aca7 mutant alleles display wild-type (WT) responses to abscisic acid (ABA) and light but are compromised in their response to elevated CO2. ACA7 is expressed in guard cells. When aca7 mutants are grown at 1000 ppm CO2 they exhibit higher transpiration and higher seed Fe and Zn content than WT grown under the same conditions. Our data show that by increasing transpiration it is possible to partially mitigate the reduction in seed Fe and Zn content when Arabidopsis is grown at elevated CO2

Climate variability and human impact in South America during the last 2000 years: synthesis and perspectives from pollen records

An improved understanding of present-day climate variability and change relies on high-quality data sets from the past 2 millennia. Global efforts to model regional climate modes are in the process of being validated against, and integrated with, records of past vegetation change. For South America, however, the full potential of vegetation records for evaluating and improving climate models has hitherto not been sufficiently acknowledged due to an absence of information on the spatial and temporal coverage of study sites. This paper therefore serves as a guide to high-quality pollen records that capture environmental variability during the last 2 millennia. We identify 60 vegetation (pollen) records from across South America which satisfy geochronological requirements set out for climate modelling, and we discuss their sensitivity to the spatial signature of climate modes throughout the continent. Diverse patterns of vegetation response to climate change are observed, with more similar patterns of change in the lowlands and varying intensity and direction of responses in the highlands. Pollen records display local-scale responses to climate modes; thus, it is necessary to understand how vegetation–climate interactions might diverge under variable settings. We provide a qualitative translation from pollen metrics to climate variables. Additionally, pollen is an excellent indicator of human impact through time. We discuss evidence for human land use in pollen records and provide an overview considered useful for archaeological hypothesis testing and important in distinguishing natural from anthropogenically driven vegetation change. We stress the need for the palynological community to be more familiar with climate variability patterns to correctly attribute the potential causes of observed vegetation dynamics. This manuscript forms part of the wider LOng-Term multi-proxy climate REconstructions and Dynamics in South America – 2k initiative that provides the ideal framework for the integration of the various palaeoclimatic subdisciplines and palaeo-science, thereby jump-starting and fostering multidisciplinary research into environmental change on centennial and millennial timescales

Le gisement épipaléolithique à pointes de Malaurie de Champ-Chalatras (Les Martres d’Artière, Puy-de-Dôme)

Le site épipaléolithique de Champ-Chalatras se caractérise par la présence de trois concentrations de vestiges archéologiques répartis autour de plusieurs foyers construits. Les analyses spatiales ont permis de mettre en évidence la structuration de l’espace domestique des locus avec des aires d’activités spécifiques liées au débitage du silex, au façonnage des outils, au rejet d’une partie des déchets de taille et à l’utilisation de certains types d’outils en relation avec les vestiges osseux. Les données fournies par l’étude archéozoologique montrent la prédominance des bovinés (Aurochs) sur les autres espèces. L’étude technologique de l’industrie lithique a permis de déterminer les modes d’introduction des différentes matières premières jurassiques, crétacées et tertiaires en fonction de leur origine géographique. Le débitage est orienté vers la production de supports laminaires rectilignes obtenus au percuteur de pierre tendre, à partir de nucléus à un ou deux plans de frappe opposés. L’outillage se caractérise par la présence de pointes à dos rectiligne et base tronquée (pointes de Malaurie), de pièces tronquées, de rectangles, de grattoirs et de pièces esquillées. Les données techno-typologiques permettent de rapprocher le site de Champ-Chalatras des niveaux laboriens des sites de la Borie del Rey et de Pont d’Ambon dans le sud-ouest de la France. Les datations au radiocarbone (10 000 ± 100 BP, 9920±120 BP et 9580±140 BP) confirment également cet état de fait, tout comme l’assemblage faunique.The Epipalaeolithic site of Champ-Chalatras is caracterised by the existence of three concentrations of archaeological pieces around several built fire places. The spatial analysis have permitted to put in evidence the domestic space structuration of locus with specific activities areas linked up flint debitage, tools shaping, part of cut wastes throwing out and the utilisation of particular tools types in relation with bone remnants. The data provided by archaeozoological study show the predominance of aurochs among the other species. The technological study of lithic industry has permitted to determine the introduction modes of different raw materials (Jurassic, Cretaceous and Tertiary) according to their geographical origins. The debitage is oriented to a straight blades production obtained with a soft stone hammer from bipolar cores. Toolkit is caracterised by the presence of Malaurie points, truncated pieces, rectangles, end-scrapers and “pièces esquillées”. Techno-typological data of Champ-Chalatras are similar to laborian levels of La Borie del Rey and Pont d’Ambon in south western France, which is confirmed by C14 dates (10 000±100 BP et 9920±120 BP)

Brazilian montane rainforest expansion induced by Heinrich Stadial 1 event

The origin of modern disjunct plant distributions in the Brazilian Highlands with strong floristic affinities to distant montane rainforests of isolated mountaintops in the northeast and northern Amazonia and the Guyana Shield remains unknown. We tested the hypothesis that these unexplained biogeographical patterns reflect former ecosystem rearrangements sustained by widespread plant migrations possibly due to climatic patterns that are very dissimilar from present-day conditions. To address this issue, we mapped the presence of the montane arboreal taxa Araucaria, Podocarpus, Drimys, Hedyosmum, Ilex, Myrsine, Symplocos, and Weinmannia, and cool-adapted plants in the families Myrtaceae, Ericaceae, and Arecaceae (palms) in 29 palynological records during Heinrich Stadial 1 Event, encompassing a latitudinal range of 30°S to 0°S. In addition, Principal Component Analysis and Species Distribution Modelling were used to represent past and modern habitat suitability for Podocarpus and Araucaria. The data reveals two long-distance patterns of plant migration connecting south/southeast to northeastern Brazil and Amazonia with a third short route extending from one of them. Their paleofloristic compositions suggest a climatic scenario of abundant rainfall and relative lower continental surface temperatures, possibly intensified by the effects of polar air incursions forming cold fronts into the Brazilian Highlands. Although these taxa are sensitive to changes in temperature, the combined pollen and speleothems proxy data indicate that this montane rainforest expansion during Heinrich Stadial 1 Event was triggered mainly by a less seasonal rainfall regime from the subtropics to the equatorial region.This work was funded by FAPESP research grant 2015/50683-2 to P.E. De Oliveira, VULPES Project, Belmount Forum

Biochemical characterisation of an α1,4 galactosyltransferase from Neisseria weaveri for the synthesis of α1,4-linked galactosides

The human cell surface trisaccharide motifs globotriose and P1 antigen play key roles in infections by pathogenic bacteria, which makes them important synthetic targets as antibacterial agents. Enzymatic strategies to install the terminal α1,4-galactosidic linkage are very attractive but have only been demonstrated for a limited set of analogues. Herein, a new bacterial α1,4 galactosyltransferase from N. weaveri was cloned and produced recombinantly in E. coli BL21 (DE3) cells, followed by investigation of its substrate specificity. We demonstrate that the enzyme can tolerate galactosamine (GalN) and also 6-deoxygalactose and 6-deoxy-6-fluorogalactose as donors, and lactose and N-acetyllactosamine as acceptors, leading directly to analogues of Gb3 and P1 that are valuable chemical probes and showcase how biocatalysis can provide fast access to a number of unnatural carbohydrate analogues