152 research outputs found
ANTESEDEN KEPUTUSAN PEMBELIAN PRODUK IPHONE PADA BRAND APPLE (STUDI KASUS PENGGUNA IPHONE DI KOTA BANDUNG)
ABSTRAK
Dari sekian banyak jenis smartphone yang beredar dipasaran, Apple merupakan salah satu vendor smartphone yang menguasai pasar Indonesia dengan salah satu perangkatnya iPhone. Pada tahun 2013 jumlah pengguna iPhone yang membeli iPhone 5s dan iPhone 5c menyentuh kurang dari 65 persen dan pada tahun berikutnya, yaitu tahun 2014 jumlah pengguna iPhone yang membeli iPhone 6 saat ini mencapai 80 persen sejak sebulan diluncurkan. Hal ini tentu menjadi sebuah fenomena karena produk iPhone yang dikenal memiliki harga yang tinggi namun tetap dapat menguasai pasar. Beberapa faktor yang mempengaruhi tingginya minat beli masyarakat diantaranya kualitas produk, harga, kepercayaan dan sikap penggunaan. Penelitian ini dilakukan kepada sampel sebanyak 350 responden dengan populasi masyarakat kota Bnadung yang menggunakan iPhone dimana penarikan sampel menggunakan metode purposive sampling. Data yang telah di kumpulkan diolah menggunakan teknik Structural Equation Modeling (SEM) engan menggunakan program Software aplikasi LISREL 8.80. hasil penelitian menunjukan bahwa kualitas produk, harga dan kepercayaan memberikan kontribusi yang besar terhadap sikap penggunaan dan sikap penggunaan dapat memoderasi kualitas produk, harga dan kepercayaan sehingga berdampak pada keputusan pembelian, sedangkan secara langsung kualitas produk, harga dan kepercayaan tidak memiliki dampak pada keputusan pembelian.
Kata kunci : kualitas produk, harga, kepercayaan, sikap penggunaan dan keputusan pembelia
Application of pharmacogenomics and bioinformatics to exemplify the utility of human <i>ex vivo</i> organoculture models in the field of precision medicine
Here we describe a collaboration between industry, the National Health Service (NHS) and academia that sought to demonstrate how early understanding of both pharmacology and genomics can improve strategies for the development of precision medicines. Diseased tissue ethically acquired from patients suffering from chronic obstructive pulmonary disease (COPD), was used to investigate inter-patient variability in drug efficacy using ex vivo organocultures of fresh lung tissue as the test system. The reduction in inflammatory cytokines in the presence of various test drugs was used as the measure of drug efficacy and the individual patient responses were then matched against genotype and microRNA profiles in an attempt to identify unique predictors of drug responsiveness. Our findings suggest that genetic variation in CYP2E1 and SMAD3 genes may partly explain the observed variation in drug response
Gene-environment interactions in sarcoidosis
Susceptibility to most human diseases is polygenic, with complex interactions between functional polymorphisms of single genes governing disease incidence, phenotype, or both. In this context, the contribution of any discrete gene is generally modest for a single individual, but may confer substantial attributable risk on a population level. Environmental exposure can modify the effects of a polymorphism, either by providing a necessary substrate for development of human disease or because the effects of a given exposure modulate the effects of the gene. In several diseases, genetic polymorphisms have been shown to be context-dependent, i.e. the effects of a genetic variant are realized only in the setting of a relevant exposure. Since sarcoidosis susceptibility is dependent on both genetic and environmental modifiers, the study of gene-environment interactions may yield important pathogenetic information and will likely be crucial for uncovering the range of genetic susceptibility loci. However, the complexity of these relationships implies that investigations of geneenvironment interactions will require the study of large cohorts with carefully-defined exposures and similar clinical phenotypes. A general principle is that the study of gene-environment interactions requires a sample size at least several-fold greater than for either factor alone. To date, the presence of environmental modifiers has been demonstrated for one sarcoidosis susceptibility locus, HLADQB1, in African-American families. This article reviews general considerations obtaining for the study of gene-environment interactions in sarcoidosis. It also describes the limited current understanding of the role of environmental influences on sarcoidosis susceptibility genes. Originally published Clinics in Dermatology, Vol. 25, No. 3, May-June 200
Dynamic gene expressions of peripheral blood mononuclear cells in patients with acute exacerbation of chronic obstructive pulmonary disease: a preliminary study
Bioinformatic analysis of microRNA and mRNA Regulation in peripheral blood mononuclear cells of patients with chronic obstructive pulmonary disease
MicroRNA and mRNA expression profiling in rat acute respiratory distress syndrome
Background: Acute respiratory distress syndrome (ARDS) is characterized by pulmonary epithelial injury and extensive inflammation of the pulmonary parenchyma. Systematic analyses of microRNA (miRNA) and mRNA expression profiling in ARDS provide insights into understanding of molecular mechanisms of the pathogenesis of ARDS. The objective of this study was to identify miRNA and mRNA interactions in a rat model of ARDS by combining miRNA and mRNA microarray analyses.Methods: Rat model of ARDS was induced by saline lavage and mechanical ventilation. The expression profiles of both mRNAs and miRNAs in rat ARDS model were performed by microarray analyses. Microarray data were further verified by quantitative RT-PCR. Functional annotation on dys-regulated mRNAs and miRNAs was carried out by bioinformatics analysis.Results: The expression of 27 miRNAs and 37 mRNAs were found to be significantly changed. The selected miRNAs and genes were further verified by quantitative real-time PCR. The down-regulated miRNAs included miR-24, miR-26a, miR-126, and Let-7a, b, c, f. The up-regulated miRNAs were composed of miR-344, miR-346, miR-99a, miR-127, miR-128b, miR-135b, and miR-30a/b. Gene ontology and functional annotation analyses indicated that up-regulated mRNAs, such as Apc, Timp1, and Sod2, were involved in the regulation of apoptosis. Bioinformatics analysis showed the inverse correlation of altered miRNAs with the expression of their predicted target mRNAs. While Sod2 was inversely correlated with Let-7a, b, c, f., Ebf1 and Apc were inversely correlated with miR-24 and miR-26a, respectively. miR-26a, miR-346, miR-135b, miR-30a/b, miR-344, and miR-18a targeted multiple altered mRNAs. Gabrb1, Sod2, Eif2ak1, Fbln5, and Tspan8 were targeted by multiple altered miRNAs.Conclusion: The expressions of miRNAs and mRNAs were altered in a rat model of ARDS. The identified miRNA-mRNA pairs may play critical roles in the pathogenesis of ARDS.Peer reviewedPathobiologyOklahoma Center for Respiratory and Infectious DiseasesPhysiological Science
Measurement of differential cross sections of isolated-photon plus heavy-flavour jet production in pp collisions at √s=8 TeV using the ATLAS detector
This Letter presents the measurement of differential cross sections of isolated prompt photons produced
in association with a b-jet or a c-jet. These final states provide sensitivity to the heavy-flavour content
of the proton and aspects related to the modelling of heavy-flavour quarks in perturbative QCD. The
measurement uses proton–proton collision data at a centre-of-mass energy of 8 TeV recorded by the
ATLAS detector at the LHC in 2012 corresponding to an integrated luminosity of up to 20.2 fb−1. The
differential cross sections are measured for each jet flavour with respect to the transverse energy of
the leading photon in two photon pseudorapidity regions: |ηγ | < 1.37 and 1.56 < |ηγ | < 2.37. The
measurement covers photon transverse energies 25 < Eγ
T < 400 GeV and 25 < Eγ
T < 350 GeV respectively
for the two |ηγ | regions. For each jet flavour, the ratio of the cross sections in the two |ηγ | regions is also
measured. The measurement is corrected for detector effects and compared to leading-order and nextto-leading-order
perturbative QCD calculations, based on various treatments and assumptions about the
heavy-flavour content of the proton. Overall, the predictions agree well with the measurement, but some
deviations are observed at high photon transverse energies. The total uncertainty in the measurement
ranges between 13% and 66%, while the central γ + b measurement exhibits the smallest uncertainty,
ranging from 13% to 27%, which is comparable to the precision of the theoretical predictions
Identification and rejection of pile-up jets at high pseudorapidity with the ATLAS detector
The rejection of forward jets originating from
additional proton–proton interactions (pile-up) is crucial for
a variety of physics analyses at the LHC, including Standard
Model measurements and searches for physics beyond
the Standard Model. The identification of such jets is challenging
due to the lack of track and vertex information in
the pseudorapidity range |η| > 2.5. This paper presents a
novel strategy for forward pile-up jet tagging that exploits
jet shapes and topological jet correlations in pile-up interactions.
Measurements of the per-jet tagging efficiency are
presented using a data set of 3.2 fb−1 of proton–proton collisions
at a centre-of-mass energy of 13 TeV collected with the
ATLAS detector. The fraction of pile-up jets rejected in the
range 2.5 < |η| < 4.5 is estimated in simulated events with
an average of 22 interactions per bunch-crossing. It increases
with jet transverse momentum and, for jets with transverse
momentum between 20 and 50 GeV, it ranges between 49%
and 67% with an efficiency of 85% for selecting hard-scatter
jets. A case study is performed in Higgs boson production
via the vector-boson fusion process, showing that these techniques
mitigate the background growth due to additional
proton–proton interactions, thus enhancing the reach for such
signatures
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