The effect of placenta previa on fetal weight and feto-maternal blood flow: a prospective cohort study

Background: The current study aims to evaluate the effect of placenta previa on the fetal weight and to explore its effect on the uterine and umbilical arteries blood flow.Methods: The current study was a prospective cohort study conducted at Assiut Women’s Health Hospital, Egypt from 1st of October 2016 to 30th of September 2017 including placenta previa and non-placenta previa women. They were followed up by two-dimensional ultrasound and Doppler blood flow in the uterine and umbilical arteries. The main study outcome was the number of low birth weight (LBW) babies delivered at or beyond 37 weeks and blood flow changes in uterine and umbilical arteries.Results: Two hundred twelve women were divided into two groups; group I included 106 placenta previa women (PP group) and 106 non-placenta previa women (NPP group). The number of LBW babies were comparable in both groups without statistically significant difference (P value= 0.555). Neither; uterine artery nor umbilical artery blood flow had any significant differences between the groups.  Preterm delivery was significantly higher in the PP group (p value=0.000).Conclusions: Although there is no agreement, in the literature, on the association between placenta previa and LBW, authors suggest that placenta previa is not a reason for LBW babies. In addition, placenta previa shows no effect on uterine artery or umbilical artery blood flow

The FGFR4-G388R Polymorphism Promotes Mitochondrial STAT3 Serine Phosphorylation to Facilitate Pituitary Growth Hormone Cell Tumorigenesis

Pituitary tumors are common intracranial neoplasms, yet few germline abnormalities have been implicated in their pathogenesis. Here we show that a single nucleotide germline polymorphism (SNP) substituting an arginine (R) for glycine (G) in the FGFR4 transmembrane domain can alter pituitary cell growth and hormone production. Compared with FGFR4-G388 mammosomatotroph cells that support prolactin (PRL) production, FGFR4-R388 cells express predominantly growth hormone (GH). Growth promoting effects of FGFR4-R388 as evidenced by enhanced colony formation was ascribed to Src activation and mitochondrial serine phosphorylation of STAT3 (pS-STAT3). In contrast, diminished pY-STAT3 mediated by FGFR4-R388 relieved GH inhibition leading to hormone excess. Using a knock-in mouse model, we demonstrate the ability of FGFR4-R385 to promote GH pituitary tumorigenesis. In patients with acromegaly, pituitary tumor size correlated with hormone excess in the presence of the FGFR4-R388 but not the FGFR4-G388 allele. Our findings establish a new role for the FGFR4-G388R polymorphism in pituitary oncogenesis, providing a rationale for targeting Src and STAT3 in the personalized treatment of associated disorders

Study of some cooking and eating quality characters on some Egyptian rice genotypes

Some Egyptian rice genotypes [i.e. Japonica (Sakha 104), Japonica/Indica (Egyptian hybrid1) and Indica (Giza 182)] were investigated to evaluate the cooking and eating quality characters. High significant differences in grain shape were observed among rice genotypes. Hulling, milling and head rice percentage were higher in Sakha 104 than other rice genotypes, while Indica type (Giza 182) recorded the lowest values in milling and physical characters. No significant differences were found in chemical composition of the three genotypes of rice was recorded, but Giza 182 had the highest protein content. All Egyptian rice genotypes were low in gelatinization temperature and soft in gel consistency. Japonica and Indica rice varieties were low in amylase content, while Japonica/Indica rice variety was intermediate. The use of RVA is considered a good index for palatability evaluation for milled rice flour and starch. The Indica and Japonica/Indica types are low in breakdown viscosity, but higher in cooked pasta than Japonica type. Japonica type recorded the best score in panel test, followed by Indica type, while Indica/Japonica rice variety was the least accepted by Egyptian consumer

Production and properties of spray dried Clinacanthus nutans using modified corn starch as drying agent

Clinacanthus nutans leaves is a medicinal plant with promising therapeutic effect. The objective of this study was to produce C. nutans powder using a spray dryer and to evaluate its physicochemical properties, followed by physical observation after 3 months of storage at room temperature (21±1°C). C. nutans juice with and without the addition of 5% (w/v) of modified corn starch (MCS) was spray dried at a feed flow rate of 23 rpm, inlet air temperature of 160°C and outlet air temperature of 100°C. Results showed that significantly lower (p <0.05) values of water activity, moisture content, flowability, particle size, solubility, wettability and dispersibility times, while significantly higher (p<0.05) values of hygroscopicity, bulk and tapped densities were recorded as compared to the spray dried powder without the addition of MCS. These indicated that MCS as drying agent has significantly improved the powder properties and the physical appearance during storage. Furthermore, produced C. nutans powder had a significantly higher (p<0.05) IC50 values, percentage of inhibition and total phenolics content. Also, a strong correlation was observed between total phenolic and antioxidant activity, indicated that encapsulation using MCS successfully protected the thermally sensitive compounds which contributed to high antioxidant activity

Identification and characterization of an inhibitory fibroblast growth factor receptor 2 (FGFR2) molecule, up-regulated in an Apert Syndrome mouse model

AS (Apert syndrome) is a congenital disease composed of skeletal, visceral and neural abnormalities, caused by dominant-acting mutations in FGFR2 [FGF (fibroblast growth factor) receptor 2]. Multiple FGFR2 splice variants are generated through alternative splicing, including PTC (premature termination codon)-containing transcripts that are normally eliminated via the NMD (nonsense-mediated decay) pathway. We have discovered that a soluble truncated FGFR2 molecule encoded by a PTC-containing transcript is up-regulated and persists in tissues of an AS mouse model. We have termed this IIIa–TM as it arises from aberrant splicing of FGFR2 exon 7 (IIIa) into exon 10 [TM (transmembrane domain)]. IIIa–TM is glycosylated and can modulate the binding of FGF1 to FGFR2 molecules in BIAcore-binding assays. We also show that IIIa–TM can negatively regulate FGF signalling in vitro and in vivo. AS phenotypes are thought to result from gain-of-FGFR2 signalling, but our findings suggest that IIIa–TM can contribute to these through a loss-of-FGFR2 function mechanism. Moreover, our findings raise the interesting possibility that FGFR2 signalling may be a regulator of the NMD pathway

Rewiring coral: Anthropogenic nutrients shift diverse coral–symbiont nutrient and carbon interactions toward symbiotic algal dominance

Improving coral reef conservation requires heightened understanding of the mechanisms by which coral cope with changing environmental conditions to maintain optimal health. We used a long‐term (10 month) in situ experiment with two phylogenetically diverse scleractinians (Acropora palmata and Porites porites) to test how coral–symbiotic algal interactions changed under real‐world conditions that were a priori expected to be beneficial (fish‐mediated nutrients) and to be harmful, but non‐lethal, for coral (fish + anthropogenic nutrients). Analyzing nine response variables of nutrient stoichiometry and stable isotopes per coral fragment, we found that nutrients from fish positively affected coral growth, and moderate doses of anthropogenic nutrients had no additional effects. While growing, coral maintained homeostasis in their nutrient pools, showing tolerance to the different nutrient regimes. Nonetheless, structural equation models revealed more nuanced relationships, showing that anthropogenic nutrients reduced the diversity of coral–symbiotic algal interactions and caused nutrient and carbon flow to be dominated by the symbiont. Our findings show that nutrient and carbon pathways are fundamentally “rewired” under anthropogenic nutrient regimes in ways that could increase corals’ susceptibility to further stressors. We hypothesize that our experiment captured coral in a previously unrecognized transition state between mutualism and antagonism. These findings highlight a notable parallel between how anthropogenic nutrients promote symbiont dominance with the holobiont, and how they promote macroalgal dominance at the coral reef scale. Our findings suggest more realistic experimental conditions, including studies across gradients of anthropogenic nutrient enrichment as well as the incorporation of varied nutrient and energy pathways, may facilitate conservation efforts to mitigate coral loss.We provide a long‐term field experiment to test the implications of different nutrient sources, fish excretion and moderate levels of anthropogenic nutrients, for coral health and coral–symbiont interactions. Our study identifies a potentially novel "transition state" whereby despite maintaining high growth rates and creating no apparent negative external effects, anthropogenic nutrient enrichment drives coral–algal interactions to be dominated by the algal symbiont—that is, increased prominence of energy and nutrient flow from the algal symbiont under conditions of Fish + anthropogenic nutrients (NPK) in the figure. We hypothesize that this “rewiring” of the coral–symbiont interactions may render the coral more vulnerable to additional stressors.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/162733/2/gcb15230_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/162733/1/gcb15230.pd

Manufactured Nano-Objects Confer Viral Protection against Cucurbit Chlorotic Yellows Virus (CCYV) Infecting Nicotiana benthamiana

Nanotechnology has emerged as a new tool to combat phytopathogens in agricultural crops. Cucurbit chlorotic yellows virus (CCYV) mainly infects Solanaceae crops and causes significant crop losses. Nanomaterials (NMs) may have efficacy against plant viruses, but the mechanisms underlying complex nanomaterials-plant-virus interactions remain elusive. We challenged Nicotiana benthamiana plants with GFP-tagged CCYV and observed morphological, physiological, and molecular changes in response to 21-d foliar exposure to nanoscale Fe and Zn and C60 fullerenes at 100 mg/L concentration for 21 days. We observed that in response to C60 (100 mg/L) treatment, plants displayed a normal phenotype while the viral infection was not seen until 5 days post-inoculation. On the contrary, Fe and Zn were unable to suppress viral progression. The mRNA transcriptional analysis for GFP and viral coat protein revealed that the transcripts of both genes were 5-fold reduced in response to C60 treatment. Evaluation of the chloroplast ultrastructure showed that NMs treatment maintained the normal chloroplast structure in the plants as compared to untreated plants. C60 upregulated the defense-related phytohormones (abscisic acid and salicylic acid) by 42–43%. Our results demonstrate the protective function of carbon-based NMs, with suppression of CCYV symptoms via inhibition of viral replication and systemic movement

Neuroprotective and Anti-Inflammatory Effects of \u3ci\u3eRhus coriaria\u3c/i\u3e Extract in a Mouse Model of Ischemic Optic Neuropathy

Modulating oxidative stresses and inflammation can potentially prevent or alleviate the pathological conditions of diseases associated with the nervous system, including ischemic optic neuropathy. In this study we evaluated the anti-neuroinflammatory and neuroprotective activities of Rhus coriaria (R. coriaria) extract in vivo. The half maximal inhibitory concentration (IC50) for DPPH, ABTS and B-carotene were 6.79 ± 0.009 µg/mL, 10.94 ± 0.09 µg/mL, and 6.25 ± 0.06 µg/mL, respectively. Retinal ischemia was induced by optic nerve crush injury in albino Balb/c mice. The anti-inflammatory activity of ethanolic extract of R. coriaria (ERC) and linoleic acid (LA) on ocular ischemia was monitored using Fluorescence Molecular Tomography (FMT). Following optic nerve crush injury, the mice treated with 400 mg/kg of ERC and LA exhibited an 84.87% and 86.71% reduction of fluorescent signal (cathepsin activity) respectively. The results of this study provide strong scientific evidence for the neuroprotective activity of the ERC, identifying LA as one of the main components responsible for the effect. ERC may be useful and worthy of further development for its adjunctive utilization in the treatment of optic neuropathy