POLA KOMUNIKASI ORANG TUA PADA ANAK BERKEBUTUHAN KHUSUS DI KOTA MANADO PROVINSI SULAWESI UTARA

Penelitian ini bertujuan untuk mengetahui pola komunikasi orangtua pada anak berkebutuhan khusus yang didasari pada konsep diri orangtua dalam memaknai kehadiran anak, motif orangtua dalam berkomunikasi serta intensitas hubungan antar orangtua dan anak berkebutuhan khusus.Metode yang digunakan adalah metode kualitatif dengan pendekatan fenomenologi. Teknik pengumpulan data berupa wawancara mendalam, dan pengamatan berperan serta dengan menggunakan teori fenomenologi dan interaksi simbolik. Penelitian ini diidentifikasikan sebagai berikut : (1) Bagaimana konsep diri orangtua dalam menyikapi kehadiran anak berkebutuhan khusus; (2) apa motif orangtua dalam berkomunikasi dengan anak berkebutuhan khusus; (3) bagaimana intensitas komunikasi orangtua dalam menjalin hubungan dengan anak berkebutuhan khusus. Berdasarkan hasil penelitian menyatakan bahwa pola komunikasi orangtua pada anak berkebutuhan khusus didasari pada konsep orientasi intensional dimana orangtua melihat keberadaan anak berkebutuhan khusus adalah anak yang tidak normal, tidak menarik dan tidak memiliki masa depan yang baik. sedangkan pola komunikasi orangtua yang didasari pada konsep orientasi ekstensional adalah orangtua yang memandang keberadaan anak melalui ciriciri-cirinya, kekhasahannya, keunikannya bahkan kelebihannya yang mungkin tidak dimiliki oleh anak normal lainnya. Orangtua yang berpandangan ekstensional adalah orangtua yang bisa menerima keberadaan anaknya dan memberikan perhatian, kasih sayang serta dukungan yang seutuhnya. Orangtua yang memiliki orientasi ekstensional dikategorikan berhasil dalam mengasuh anak berkebutuhan khusus dibandingkan dengan orangtua orientasi intensional yang hanya melihat keberadaan anaknya dari sisi kekurangan dan kelemahannya._____________________________________________________________________________Kata Kunci : Pola, Komunikasi, Orangtua, Anak, berkebutuhan khusus

Finding least fragmented holdings with factor analysis and a new methodology: a case study of kargılı land consolidation project from Turkey

ArticleLand fragmentation (LF) is a problem restrain agricultural activities and decrease mechanization level, production. Land consolidation (LC) projects are done in the World as well as Turkey to solve LF issues. Researchers created indicators to measure land fragmentation which is important to see success level of LC projects. The use of these indicators is controversial or not accurate. The core aim of the present study is to find new land fragmentation index and to find least fragmented holding with factor analysis using the other indicators which are Simmons, Januszevski, number of parcels, Shmook and Igbozurike besides new land fragmentation index. Kargılı Village land consolidation project in Mersin, Turkey was chosen as a material. Cadastral data before land consolidation, was used to calculate value of indicators, where number of parcels was 932, total area was 1,741.9 ha, the average parcel size was 1.9 ha, number of holdings was 542 and the average parcel size was village had 932 parcels. Data processing were performed with ArcMAP 10.6.1 and SPSS. A total of 18 holdings were identified randomly as sample size which were sufficient to carry out factor analysis including principle component to rank holdings (P < 0.01).As a result, new land fragmentation index found correlated with others (P < 0.01) and ranking according to new indicator performed better than ranking considering all indicators. In this context, it is possible to use new land fragmentation indicator to determine priority areas for land consolidation

Land Cover Based on Geophysical Characteristics in the Mount Sirimau Protection Forest Group, Ambon City, Maluku Province

The protected forest as life support ideally has a good performance in this case its vegetation, so that its main function can run well. However, as a forest with various functions, the protected forest of Ambon City, especially the Mount Sirimau Protection Forest Group as a strategic protected forest is located upstream of Ambon City which is very influential on the activities of Ambon City as its downstream, because it has a social and economic impact. The performance of a forest area can be seen from its land cover. In addition, the geophysical characteristics of an area will also affect the land cover of the area. Therefore, the purpose of this study was to determine the condition of land cover in the Mount Sirimau Protection Forest Group based on its geophysical characteristics. The research method used was the survey method and the data analysis method was descriptive analysis. The land covers in the Mount Sirimau Protection Forest Group consisted of seven land covers, spreading over soil types, rock types, topography, and slopes. Intensive management of protected forests is needed according to their geophysical characteristics so that the function of protected forests can be more optimal. &nbsp

Chemical profiling and cholinesterase inhibitory activity of five Phaedranassa Herb. (Amaryllidaceae) species from Ecuador

It is estimated that 50 million people in the world live with dementia, 60-70% of whom suffer from Alzheimer's disease (AD). Different factors are involved in the development of AD, including a reduction in the cholinergic neurotransmission level. The Amaryllidaceae plant family contains an exclusive, large, and still understudied alkaloid group characterized by a singular skeleton arrangement and a broad spectrum of biological activities. The chemistry and biodiversity of Ecuadorian representatives of the Phaedranassa genus (Amaryllidaceae) have not been widely studied. In this work, five Ecuadorian Phaedranassa species were examined in vitro for their activity towards the enzymes acetyl- (AChE) and butyrylcholinesterase (BuChE), and the alkaloid profile of bulb extracts was analyzed by GC-MS. The species Phaedranassa cuencana and Phaedranassa dubia showed the most AChE and BuChE inhibitory activity, respectively. To obtain insight into the potential role of the identified alkaloids in these inhibitory effects, docking experiments were carried out, and cantabricine showed in silico inhibitory activity against both cholinesterase structures. Our results show that Amaryllidaceae species from Ecuador are a potential source of new drugs for the palliative treatment of AD

Asymmetrical flow field-flow fractionation in purification of an enveloped bacteriophage φ 6

Basic and applied virus research requires specimens that are purified to high homogeneity. Thus, there is much interest in the efficient production and purification of viruses and their subassemblies. Advances in the production steps have shifted the bottle neck of the process to the purification. Nonetheless, the development of purification techniques for different viruses is challenging due to the complex biological nature of the infected cell cultures as well as the biophysical and -chemical differences in the virus particles. We used bacteriophage phi 6 as a model virus in our attempts to provide a new purification method for enveloped viruses. We compared asymmetrical flow field-flow fractionation (AF4)-based virus purification method to the well-established ultracentrifugation-based purification of phi 6. In addition, binding of phi 6 virions to monolithic anion exchange columns was tested to evaluate their applicability in concentrating the AF4 purified specimens. Our results show that AF4 enables one-hour purification of infectious enveloped viruses with specific infectivity of similar to 1 x 10(13) PFU/mg of protein and similar to 65-95% yields. Obtained purity was comparable with that obtained using ultracentrifugation, but the yields from AF4 purification were 2-3-fold higher. Importantly, high quality virus preparations could be obtained directly from crude cell lysates. Furthermore, when used in combination with inline light scattering detectors, AF4 purification could be coupled to simultaneous quality control of obtained virus specimen.Peer reviewe

High-throughput isolation and characterization of untagged membrane protein complexes: outer membrane complexes of Desulfovibrio vulgaris.

Cell membranes represent the "front line" of cellular defense and the interface between a cell and its environment. To determine the range of proteins and protein complexes that are present in the cell membranes of a target organism, we have utilized a "tagless" process for the system-wide isolation and identification of native membrane protein complexes. As an initial subject for study, we have chosen the Gram-negative sulfate-reducing bacterium Desulfovibrio vulgaris. With this tagless methodology, we have identified about two-thirds of the outer membrane- associated proteins anticipated. Approximately three-fourths of these appear to form homomeric complexes. Statistical and machine-learning methods used to analyze data compiled over multiple experiments revealed networks of additional protein-protein interactions providing insight into heteromeric contacts made between proteins across this region of the cell. Taken together, these results establish a D. vulgaris outer membrane protein data set that will be essential for the detection and characterization of environment-driven changes in the outer membrane proteome and in the modeling of stress response pathways. The workflow utilized here should be effective for the global characterization of membrane protein complexes in a wide range of organisms

A tuberculosis molecular bacterial load assay (TB-MBLA)

Funding: European and Developing Countries Clinical Trials Partnership (EDCTP) – Pan African Biomarker Expansion program (PanBIOME) grant SP.2011.41304.008. Support was also obtained the University of St Andrews School of Medicine research grant.Tuberculosis is caused by Mycobacterium tuberculosis (Mtb), a pathogen classified by the United Nations (UN) as a dangerous category B biological substance. For the sake of the workers’ safety, handling of all samples presumed to carry Mtb must be conducted in a containment level (CL) 3 laboratory. The TB molecular bacterial load assay (TB-MBLA) test is a reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) test that quantifies Mtb bacillary load using primers and dual-labelled probes for 16S rRNA. We describe the use of heat inactivation to render TB samples noninfectious while preserving RNA for the TB-MBLA. A 1 mL aliquot of the sputum sample in tightly closed 15 mL centrifuge tubes is boiled for 20 min at either 80 °C, 85 °C, or 95 °C to inactivate Mtb bacilli. Cultivation of the heat inactivated and control (live) samples for 42 days confirmed the death of TB. The inactivated sample is then spiked with 100 µL of the extraction control and RNA is extracted following the standard RNA isolation procedure. No growth was observed in the cultures of heat treated samples. The isolated RNA is subjected to real-time RT-qPCR, which amplifies a specific target in the Mtb 16S rRNA gene, yielding results in the form of quantification cycles (Cq). A standard curve is used to translate Cq into bacterial load, or estimated colony forming units per mL (eCFU/mL). There is an inverse relationship between Cq and the bacterial load of a sample. The limitation is that heat inactivation lyses some cells, exposing the RNA to RNases that cause a loss of <1 log10eCFU/mL (i.e., <10 CFU/mL). Further studies will determine the proportion of very low burden patients that cause false negative results due to heat inactivation.Publisher PDFPeer reviewe

Sosialisasi Pengenalan Dan Pencegahan Diabetes Melitus

Diabetes melitus didefinisikan sebagai kelainan metabolik dengan berbagai etiologi yang ditandai dengan hiperglikemia kronis dengan gangguan metabolisme karbohidrat, protein dan lemak yang dihasilkan dari defek pada sekresi insulin, kerja insulin, atau keduanya. Diabetes melitus saat ini menjadi salah satu ancaman kesehatan global. DM dapat diklasifikasikan menjadi 4 kelompok, yaitu DM tipe 1, DM tipe 2, DM gestasional dan DM tipe lain. Penemuan obat diabetes mellitus yang diciptakan juga semakin banyak namun terkadang memiliki efek samping yang tidak kita inginkan. Sehingga melalui penyuluhan ini dapat dimanfaatkan sebaik mungkin agar masyarakat dapat memahami bahwa penting sekali untuk menjaga pola sehat agar terhindar dari penyakit diabetes mellitus. Selama acara berlangsung, Proses sosialisasi berlangsung dengan sangat baik. Untuk memastikan para peserta sosialisasi memahami dan mengerti materi yang telah disampaikan, dilakukan evaluasi pertanyaan mengenai materi dan meminta para peserta untuk mengulang kembali materi yang telah dijelaskan. Sehingga diharapkan para peserta yang lainnya lebih antusias dalam mengikuti penyuluhan dan sesi tanya jawab terhadap materi yang telah disampaika

Effect of seven anti-tuberculosis treatment regimens on sputum microbiome : a retrospective analysis of the HIGHRIF study 2 and PanACEA MAMS-TB clinical trials

Funding: European and Developing Countries Clinical Trials Partnership and German Ministry of Education and Research.Background Respiratory tract microbiota has been described as the gatekeeper for respiratory health. We aimed to assess the impact of standard-of-care and experimental anti-tuberculosis treatment regimens on the respiratory microbiome and implications for treatment outcomes. Methods In this retrospective study, we analysed the sputum microbiome of participants with tuberculosis treated with six experimental regimens versus standard-of-care who were part of the HIGHRIF study 2 (NCT00760149 ) and PanACEA MAMS-TB (NCT01785186 ) clinical trials across a 3-month treatment follow-up period. Samples were from participants in Mbeya, Kilimanjaro, Bagamoyo, and Dar es Salaam, Tanzania. Experimental regimens were composed of different combinations of rifampicin (R), isoniazid (H), pyrazinamide (Z), ethambutol (E), moxifloxacin (M), and a new drug, SQ109 (Q). Reverse transcription was used to create complementary DNA for each participant's total sputum RNA and the V3-V4 region of the 16S rRNA gene was sequenced using the Illumina metagenomic technique. Qiime was used to analyse the amplicon sequence variants and estimate alpha diversity. Descriptive statistics were applied to assess differences in alpha diversity pre-treatment and post-treatment initiation and the effect of each treatment regimen. Findings Sequence data were obtained from 397 pre-treatment and post-treatment samples taken between Sept 26, 2008, and June 30, 2015, across seven treatment regimens. Pre-treatment microbiome (206 genera) was dominated by Firmicutes (2860 [44%] of 6500 amplicon sequence variants [ASVs]) at the phylum level and Streptococcus (2340 [36%] ASVs) at the genus level. Two regimens had a significant depressing effect on the microbiome after 2 weeks of treatment, HR20mg/kgZM (Shannon diversity index p=0·0041) and HR35mg/kgZE (p=0·027). Gram-negative bacteria were the most sensitive to bactericidal activity of treatment with the highest number of species suppressed being under the moxifloxacin regimen. By week 12 after treatment initiation, microbiomes had recovered to pre-treatment level except for the HR35mg/kgZE regimen and for genus Mycobacterium, which did not show recovery across all regimens. Tuberculosis culture conversion to negative by week 8 of treatment was associated with clearance of genus Neisseria, with a 98% reduction of the pre-treatment level. Interpretation HR20mg/kgZM was effective against tuberculosis without limiting microbiome recovery, which implies a shorter efficacious anti-tuberculosis regimen with improved treatment outcomes might be achieved without harming the commensal microbiota.Publisher PDFPeer reviewe