Relaciones intergeneracionales en la escuela: el proyecto Compartir la Infancia

El trabajo que se presenta es la investigación generada por el programa Compartiendo Infancias o Sharing Childhood (SACHI) desarrollado en dos fases. La primera (SACHI 1, 2013-2015) en el marco de Lifelong Learning de la Comisión Europea (Grundtvig) y la segunda (SACHI 2, 2016-2018) en el marco de los «Proyectos de Asociaciones Estratégicas orientadas al campo de la Educación de Personas Adultas (KA2)» de la Comisión Europea (convenio 2016-1-ES01-KA204-024999).8 Coordinado por el Grupo de Investigación y Formación Educativa y Social (GIFES) de la Universidad de las Islas Baleares (España), ha contado en la primera fase con la colaboración de Foundation for the Development of the Education System HIPOKAMP (Łódź, Polonia) y Bayat Halk Egitim Merkezi Mudurlugu (Afyonkarahisar, Turquía). En la segunda fase, los socios son Association Educational Center for Intergenerational Integration Hipocamp (Łódź, Polonia), University of Strathclyde (Glasgow, Reino Unido), Universidade do Porto (Porto, Portugal). El objetivo principal del proyecto es fomentar las relaciones positivas entre niños y niñas (10-12) y personas mayores de 50 años. Los objetivos específicos son generar un beneficio recíproco, compartir conocimientos y habilidades, adquirir valores positivos y diseminar una metodología intergeneracional en centros educativos. En los resultados del proyecto se han podido constatar beneficios para todos los colectivos participantes. Principalmente se pueden destacar, junto al cambio de actitudes, la transformación de las relaciones (experiencias, lazos afectivos, creencias y valores), aumento de la autoestima y el autoconcepto y una mayor motivación hacia el aprendizaje de jóvenes y mayores, formación permanente de los profesionales, aumento de la participación familiar y comunitaria y aumento del trabajo en red y creación de nuevos contactos profesionales

«Compartir la infància 2» (SACHI 2) projecte intergeneracional: l’experiència en tres escoles de Palma (2016-2018)

[cat] «Compartir la infància 2» (SACHI 2) és un projecte intergeneracional finançat per ERASMUS + (convocatòria de 2016-2017) i liderat pel Grup de Recerca i Formació Educativa i Social (GIFES). En aquest capítol es descriu la implementació realitzada en tres centres educatius de la ciutat de Palma i es posa en relleu el seu potencial per facilitar la cohesió social necessària per mantenir el pacte intergeneracional de la nostra societat i, per la satisfacció directa dels participants, la bona acollida que s’ha pogut demostrar que té. GIFES té una llarga trajectòria desenvolupant espais de participació de gent gran vinculats amb cursos de la UOM (Universitat Oberta per a Majors) i analitzant experiències intergeneracionals de fora de la universitat, algunes ja descrites al llarg dels anuaris dels darrers deu anys. SACHI, incloses les experiències 1 i 2, recull aquest bagatge i representa una aposta per un projecte que promou les relacions positives entre infants i grans, a fi de prevenir l’edatisme des dels centres educatius de primària. A més a més, es tracta d’un projecte estructurat i contrastat, com a projecte de investigació, mitjançant l’avaluació d’aquestes experiències pilot. Aquí s’ofereix l’oportunitat de conèixer beneficis i aportacions del projecte en el marc de: (a) l’envelliment actiu; (b) la millora de les actituds en vers la gent gran, i (c) la inclusió de la perspectiva intergeneracional als centres educatius: conèixer claus del procés d’implementació que s’ha realitzat durant els darrers dos cursos escolars a Palma.[spa] «Compartir la infancia 2» (SACHI 2) es un proyecto intergeneracional financiado por ERASMUS + (convocatoria de 2016-2017) y liderado por el Grupo de Investigación y Formación Educativa y Social (GIFES). En este artículo se describe la implementación realizada en tres centros educativos de la ciudad de Palma y se pone de relieve su potencial para facilitar la cohesión social necesaria para mantener el pacto intergeneracional de nuestra sociedad y, a nivel de satisfacción directa de los participantes, la buena acogida que se ha podido mostrar que tiene. GIFES tiene una larga trayectoria desarrollando espacios de participación de persones mayores vinculados a cursos de la UOM (Universitat Oberta per a Majors) y analizando experiencias intergeneracionales de fuera de la universidad, algunas de ellas descritas a lo largo de los anuarios de los últimos diez años. SACHI, incluyendo las experiencias 1 y 2, recoge este bagaje y representa una apuesta por un proyecto que promueve las relaciones positivas entre niños/as y personas mayores, a fin de prevenir el edadismo desde los centres educativos de primaria. Además, se trata de un proyecto estructurado y contrastado, como proyecto de investigación, mediante la evaluación de estas experiencias piloto

In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network

The purpose of the present work is to underline the importance of obtaining a standardized procedure to ensure and evaluate both clinical and research usability of human tissue samples. The study, which was carried out by the Biospecimen Science Working Group of the Spanish Biobank Network, is based on a general overview of the current situation about quality assurance in human tissue biospecimens. It was conducted an exhaustive review of the analytical techniques used to evaluate the quality of human tissue samples over the past 30 years, as well as their reference values if they were published, and classified them according to the biomolecules evaluated: (i) DNA, (ii) RNA, and (iii) soluble or/and fixed proteins for immunochemistry. More than 130 publications released between 1989 and 2019 were analysed, most of them reporting results focused on the analysis of tumour and biopsy samples. A quality assessment proposal with an algorithm has been developed for both frozen tissue samples and formalin-fixed paraffin-embedded (FFPE) samples, according to the expected quality of sample based on the available pre-analytical information and the experience of the participants in the Working Group. The high heterogeneity of human tissue samples and the wide number of pre-analytic factors associated to quality of samples makes it very difficult to harmonize the quality criteria. However, the proposed method to assess human tissue sample integrity and antigenicity will not only help to evaluate whether stored human tissue samples fit for the purpose of biomarker development, but will also allow to perform further studies, such as assessing the impact of different pre-analytical factors on very well characterized samples or evaluating the readjustment of tissue sample collection, processing and storing procedures. By ensuring the quality of the samples used on research, the reproducibility of scientific results will be guaranteed.This work was funded by the Ministerio de Ciencia, Innovacion y Universidades of Spain and Instituto de Salud Carlos III (PI16/00528, PI16/00946, PI16/01207 and PI16/01276), co-funded by the Spanish Biobank Network (PT13/0010/0030, PT17/0015/0001, PT17/0015/0021, PT17/0015/0049, PT17/0015/0018, PT17/0015/0002, PT17/0015/0016, PT17/0015/0038, PT17/0015/0027, PT17/0015/0004, PT17/0015/0047, PT17/0015/0014, PT17/0015/0041, and PT17/0015/0006), European Regional Development Fund (FEDER) "A way to make Europe" and granted by Conselleria d'Innovacio, Recerca i Turisme del Govern de les Illes Balears (TEC/002/2017).S

METTL3 as a master regulator of translation in cancer : mechanisms and implications

Translational regulation is an important step in the control of gene expression. In cancer cells, the orchestration of both global control of protein synthesis and selective translation of specific mRNAs promote tumor cell survival, angiogenesis, transformation, invasion and metastasis. N6-methyladenosine (m6A), the most prevalent mRNA modification in higher eukaryotes, impacts protein translation. Over the past decade, the development of m6A mapping tools has facilitated comprehensive functional investigations, revealing the involvement of this chemical mark, together with its writer METTL3, in promoting the translation of both oncogenes and tumor suppressor transcripts, with the impact being context-dependent. This review aims to consolidate our current understanding of how m6A and METTL3 shape translation regulation in the realm of cancer biology. In addition, it delves into the role of cytoplasmic METTL3 in protein synthesis, operating independently of its catalytic activity. Ultimately, our goal is to provide critical insights into the interplay between m6A, METTL3 and translational regulation in cancer, offering a deeper comprehension of the mechanisms sustaining tumorigenesis

Impact of different stabilization methods on RT-qPCR results using human lung tissue samples.

Aiming to increase the reproducibility of biomedical research results, biobanks obtain human tissues of the highest quality and carry out different storage methods adapted to the needs of analytical technique to be performed by the biomedical researchers. However, there is much controversy and little data concerning the real impact of different stabilization methods on tissue quality, integrity and functionality of derived biomolecules. The influence of four stabilization methods [RNAlater (RNL), snap freezing (SF), snap freezing using Optimal Cutting Tissue compound (SF-OCT) and formalin-fixed paraffin-embedded (FFPE)] on RNA quality and integrity was evaluated in paired samples of lung tissue. RNA integrity was evaluated through PCR-endpoint assays amplifying six fragments of different length of the HPRT1 gene and RNA Integrity Number (RIN). To evaluate the difference of tissue functionality among the stabilization methods tested, RT-qPCRs were performed focusing on the differential expression of the HPRT1, SNRPD3 and Jun genes. RNA from the samples preserved with the RNL or SF-OCT method showed better integrity compared to SF and FFPE, measured by PCR-endpoint and RT-qPCR assays. However, only statistically significant differences were observed between the RNA from FFPE and other stabilization methods when gene expression of HPRT1, SNRPD3 and Jun housekeeping genes were determined by RT-qPCR. For the three mentioned genes, Cq and RIN values were highly correlated. The present work describes the fragility of SF samples, being critical the moment just before RNA extraction, although further experiments of tissue RNA are needed. Standardization pre-analytic workflow can lead to improved reproducibility between biomedical research studies. The present study demonstrated clear evidences about the impact of the stabilization method on RNA derived from lung human tissue samples

Impact of different stabilization methods on RT-qPCR results using human lung tissue samples

[eng] Aiming to increase the reproducibility of biomedical research results, biobanks obtain human tissues of the highest quality and carry out different storage methods adapted to the needs of analytical technique to be performed by the biomedical researchers. However, there is much controversy and little data concerning the real impact of different stabilization methods on tissue quality, integrity and functionality of derived biomolecules. The influence of four stabilization methods [RNAlater (RNL), snap freezing (SF), snap freezing using Optimal Cutting Tissue compound (SF-OCT) and formalin-fixed paraffin-embedded (FFPE)] on RNA quality and integrity was evaluated in paired samples of lung tissue. RNA integrity was evaluated through PCR-endpoint assays amplifying six fragments of different length of the HPRT1 gene and RNA Integrity Number (RIN). To evaluate the difference of tissue functionality among the stabilization methods tested, RT-qPCRs were performed focusing on the differential expression of the HPRT1, SNRPD3 and Jun genes. RNA from the samples preserved with the RNL or SF-OCT method showed better integrity compared to SF and FFPE, measured by PCR-endpoint and RT-qPCR assays. However, only statistically significant differences were observed between the RNA from FFPE and other stabilization methods when gene expression of HPRT1, SNRPD3 and Jun housekeeping genes were determined by RT-qPCR. For the three mentioned genes, Cq and RIN values were highly correlated. The present work describes the fragility of SF samples, being critical the moment just before RNA extraction, although further experiments of tissue RNA are needed. Standardization pre-analytic workflow can lead to improved reproducibility between biomedical research studies. The present study demonstrated clear evidences about the impact of the stabilization method on RNA derived from lung human tissue samples

Hsa-Mir-320c, Hsa-Mir-200c-3p, and Hsa-Mir-449c-5p as Potential Specific miRNA Biomarkers of COPD : A Pilot Study

Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease commonly induced by cigarette smoke. The expression of miRNAs can be altered in patients with COPD and could be used as a biomarker. We aimed to identify a panel of miRNAs in bron-choalveolar lavage (BAL) to differentiate COPD patients from smokers and non-smokers with normal lung function. Accordingly, forty-five subjects classified as COPD, smokers, and non-smokers (n = 15 per group) underwent clinical, functional characterization and bronchoscopy with BAL. The mean age of the studied population was 61.61 ± 12.95 years, BMI 25.72 ± 3.82 Kg/m2, FEV1/FVC 68.37 ± 12.00%, and FEV1 80.07 ± 23.63% predicted. According to microarray analysis, three miRNAs of the most upregulated were chosen: miR-320c, miR-200c-3p, and miR-449c-5p. These miRNAs were validated by qPCR and were shown to be differently expressed in COPD patients. ROC analysis showed that these three miRNAs together had an area under the curve of 0.89 in differentiating COPD from controls. Moreover, in silico analysis of candidate miRNAs by DIANA-miRPath showed potential involvement in the EGFR and Hippo pathways. These results suggest a specific 3-miRNA signature that could be potentially used as a biomarker to distinguish COPD patients from smokers and non-smoker subjects

Detection of the EGFR G719S mutation in non-small cell lung cancer using Droplet Digital PCR (under review)

[eng] Objectives: The main objectives of the study were 1) to set-up a droplet digital PCR (ddPCR) assay 25 for the non-invasive detection of G719S EGFR mutation in NSCLC patients; 2) to determine the 26 limits of detection of the ddPCR assay for G719S mutation and 3) to compare COBAS® and ddPCR 27 System for G719S quantification in plasma. Materials and methods: Blood samples were collected from 19 patients diagnosed with clinical 29 stage IVA or IVB NSCLC according to the TNM Classification of Malignant Tumors. Then, plasma 30 ctDNA was extracted with the Qiagen Circulating Nucleic Acids kit and quantified by QuantiFluor® 31 dsDNA System. The mutational study of EGFR was carried out by digital droplet PCR (ddPCR) with 32 the QX200 Droplet Digital PCR System with specific probes and primers. Results: We observed the lowest percentage of G719S mutant allele could be detected in a wildtype 34 background was 0,058%. In the specificity analysis, low levels of G719S mutation were detected in 35 healthy volunteers with a peak of 21.65 mutant copies per millilitre of plasma and 6.35 MAFs. In 36 those patients whose tissue biopsy was positive for G719S mutation, mutant alleles could also be 37 detected in plasma using both ddPCR and COBAS® System. Finally, when mutational status was 38 studied using both genotyping techniques, higher mutant copies/ml and higher mutant allele fraction 39 (MAF) correlated with higher Semiquantitative Index obtained by COBAS®. Conclusions: Although tissue biopsies cannot be replaced due to the large amount of information 41 they provide regarding tumor type and structure, liquid biopsy and ddPCR represents a new 42 promising strategy for genetic analysis of tumors from plasma samples. In the present study, G719S 43 mutation was detected in a highly sensitive manner, allowing its monitorization with a non-invasive 44 technique

In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue biospecimen research working group of the spanish biobank network

The purpose of the present work is to underline the importance of obtaining a standardized procedure to ensure and evaluate both clinical and research usability of human tissue samples. The study, which was carried out by the Biospecimen Science Working Group of the Spanish Biobank Network, is based on a general overview of the cur‑ rent situation about quality assurance in human tissue biospecimens. It was conducted an exhaustive review of the analytical techniques used to evaluate the quality of human tissue samples over the past 30 years, as well as their reference values if they were published, and classifed them according to the biomolecules evaluated: (i) DNA, (ii) RNA, and (iii) soluble or/and fxed proteins for immunochemistry. More than 130 publications released between 1989 and 2019 were analysed, most of them reporting results focused on the analysis of tumour and biopsy samples. A qual‑ ity assessment proposal with an algorithm has been developed for both frozen tissue samples and formalin-fxed parafn-embedded (FFPE) samples, according to the expected quality of sample based on the available pre-analytical information and the experience of the participants in the Working Group. The high heterogeneity of human tissue samples and the wide number of pre-analytic factors associated to quality of samples makes it very difcult to harmo‑ nize the quality criteria. However, the proposed method to assess human tissue sample integrity and antigenicity will not only help to evaluate whether stored human tissue samples ft for the purpose of biomarker development, but will also allow to perform further studies, such as assessing the impact of diferent pre-analytical factors on very well characterized samples or evaluating the readjustment of tissue sample collection, processing and storing procedur