104 research outputs found
Thermal-oxidation of epoxy/amine followed by glass transition temperature changes
Thermal oxidation of three epoxy resins differing by the nature of prepolymer (bisphenol A diglycidyl ether and 1,4-butanediol diglycidyl ether) and hardener (isophorone diamine and 4,7,10-Trioxa-1,13-tridecanediamine) was studied by monitoring changes in glass transition temperature using DSC. Results were discussed using the DiMarzio's approach in which parameters are estimated from an additive group contribution. This theory allowed a fair assessment of Tg values for unaged networks. During oxidation, epoxy networks were shown to undergo chain scissions occurring in great part in hydroxypropyl ether and isophorone groups. However, the exploitation of Tg changes showed the coexistence and even the predominance of crosslinking in materials having linear aliphatic segments. The DiMarzio's approach was used to discuss the possibility of intramolecular cyclization or intermolecular crosslinks which were shown to predominate. Crosslinks were tentatively justified from a mechanistic point of view and quantified depending on experimental conditions
Prediction of stress induced by heterogeneous oxidation: Case of epoxy/amine networks
A methodology to predict the formation of superficial stress during the diffusion-limited oxidation of thick epoxy/amine samples is proposed. This quantitative methodology is based on the understanding of mechanisms responsible for this stress lead to superficial micro-cracks. The first step consists of simulating the homogeneous oxidation on the surface of thick samples. The extent of oxidation is assessed through the concentration of oxidation products as amide groups measured using Fourier-transform infrared spectroscopy (FTIR). These experimental results are compared to simulations obtained from a kinetic model based on a close loop mechanistic scheme in chain oxidation. Shrinkage and tensile behavior changes are correlated with an oxidation tracer, being amide groups in our case. The second step considers diffusion-limited oxidation in thick samples where the gradients of oxidation products characterized through thickness by micro-FTIR are simulated by coupling the previous kinetic model with oxygen diffusion. Finally, the gradient of strain and stress induced by oxidation of a thick epoxy/amine sample is simulated by inserting the mechanical behavior modifications and shrinkage gradient associated with the amide gradient in finite element code (Abaqus®). As a result, it is shown that it is possible to predict the superficial stress level as a function time/temperature of exposure and sample geometry.ANRT (CIFRE No 2013/0356
Effectiveness of the capsaicin 8% patch in the management of peripheral neuropathic pain in European clinical practice: the ASCEND study
Background
In randomised studies, the capsaicin 8% patch has demonstrated effective pain relief in patients with peripheral neuropathic pain (PNP) arising from different aetiologies.
Methods
ASCEND was an open-label, non-interventional study of patients with non-diabetes-related PNP who received capsaicin 8% patch treatment, according to usual clinical practice, and were followed for ≤52 weeks. Co-primary endpoints were percentage change in the mean numeric pain rating scale (NPRS) ‘average daily pain’ score from baseline to the average of Weeks 2 and 8 following first treatment; and median time from first to second treatment. The primary analysis was intended to assess analgesic equivalence between post-herpetic neuralgia (PHN) and other PNP aetiologies. Health-related quality of life (HRQoL, using EQ-5D), Patient Global Impression of Change (PGIC) and tolerability were also assessed.
Results
Following first application, patients experienced a 26.6% (95% CI: 23.6, 29.62; n = 412) reduction in mean NPRS score from baseline to Weeks 2 and 8. Equivalence was demonstrated between PHN and the neuropathic back pain, post-operative and post-traumatic neuropathic pain and ‘other’ PNP aetiology subgroups. The median time from first to second treatment was 191 days (95% CI: 147, 235; n = 181). Forty-four percent of all patients were responders (≥30% reduction in NPRS score from baseline to Weeks 2 and 8) following first treatment, and 86.9% (n = 159/183) remained so at Week 12. A sustained pain response was observed until Week 52, with a 37.0% (95% CI: 31.3, 42.7; n = 176) reduction in mean NPRS score from baseline. Patients with the shortest duration of pain (0–0.72 years) experienced the highest pain response from baseline to Weeks 2 and 8. Mean EQ-5D index score improved by 0.199 utils (responders: 0.292 utils) from baseline to Week 2 and was maintained until Week 52. Most patients reported improvements in PGIC at Week 2 and at all follow-up assessments regardless of number of treatments received. Adverse events were primarily mild or moderate reversible application site reactions.
Conclusion
In European clinical practice, the capsaicin 8% patch provided effective and sustained pain relief, substantially improved HRQoL, improved overall health status and was generally well tolerated in a heterogeneous PNP population
CDK5RAP2 primary microcephaly is associated with hypothalamic, retinal and cochlear developmental defects
International audienc
Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes
BACKGROUND: Toxoplasmosis is an infectious disease caused by the parasitic protozoan Toxoplasma gondii. It is endemic worldwide and, depending on the geographic location, 15 to 85% of the human population are asymptomatically infected. Routine diagnosis is based on serology. The parasite has emerged as a major opportunistic pathogen for immunocompromised patients, in whom it can cause life-threatening disease. Moreover, when a pregnant woman develops a primary Toxoplasma gondii infection, the parasite may be transmitted to the fetus and cause serious damnage. For these two subpopulations, a rapid and accurate diagnosis is required to initiate treatment. Serological diagnosis of active infection is unreliable because reactivation is not always accompanied by changes in antibody levels, and the presence of IgM does not necessarily indicate recent infection. Application of quantitative PCR has evolved as a sensitive, specific, and rapid method for the detection of Toxoplasma gondii DNA in amniotic fluid, blood, tissue samples, and cerebrospinal fluid. METHODS: Two separate, real-time fluorescence PCR assays were designed and evaluated with clinical samples. The first, targeting the 35-fold repeated B1 gene, and a second, targeting a newly described multicopy genomic fragment of Toxoplasma gondii. Amplicons of different intragenic copies were analyzed for sequence heterogeneity. RESULTS: Comparative LightCycler experiments were conducted with a dilution series of Toxoplasma gondii genomic DNA, 5 reference strains, and 51 Toxoplasma gondii-positive amniotic fluid samples revealing a 10 to 100-fold higher sensitivity for the PCR assay targeting the newly described 529-bp repeat element of Toxoplasma gondii. CONCLUSION: We have developed a quantitative LightCycler PCR protocol which offer rapid cycling with real-time, sequence-specific detection of amplicons. Results of quantitative PCR demonstrate that the 529-bp repeat element is repeated more than 300-fold in the genome of Toxoplasma gondii. Since individual intragenic copies of the target are conserved on sequence level, the high copy number leads to an ultimate level of analytical sensitivity in routine practice. This newly described 529-bp repeat element should be preferred to less repeated or more divergent target sequences in order to improve the sensitivity of PCR tests for the diagnosis of toxoplasmosis
Effectiveness of the capsaicin 8% patch in the management of peripheral neuropathic pain in European clinical practice: the ASCEND study
Polymethacrylates.Material Selection For Medical Applications:Requirements For Several Kinds of Medical Applications
This chapter reviews several cases of methacrylate-based polymers used for medical applications. The main chemicals and fillers used for elaborating biomaterials are presented, together with the main synthesis reactions. Their properties are recalled and discussed using the well-established structure-properties relationships of polymer physicochemistry. Last, the main degradation mechanisms are recalled, together with their consequences on the engineering properties of polymethacrylates, in order to predict the long-term in vivo behavior of such complex materials
Adresse de l' Académie Bretonne à l' Académie Basque
Escrito de la Academia Bretona, recientemente formada, dirigido a la Academia Vasca en el que se expone la situación del bretón, desde sus dialectos, las zonas en las que se habla, el peligro de desaparición, etc.Communiqué from the Breton Academy, recently formed, to the Basque Academy on the situation of the Breton language, its dialects, the areas in which it is spoken, the danger of disappearance, etc
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