Detection of reactive oxygen metabolites in malignant and adjacent normal tissues of patients with lung cancer

BACKGROUND: Different types of reactive oxygen metabolites (ROMs) are known to be involved in carcinogenesis. Several studies have emphasized the formation of ROMs in ischemic tissues and in cases of inflammation. The increased amounts of ROMs in tumor tissues can either be because of their causative effects or because they are produced by the tumor itself. Our study aimed to investigate and compare the levels of ROMs in tumor tissue and adjacent lung parenchyma obtained from patients with lung cancer. METHODS: Fifteen patients (all male, mean age 63.6 ± 9 years) with non-small cell lung cancer were enrolled in the study. All patients were smokers. Of the patients with lung cancer, twelve had epidermoid carcinoma and three had adenocarcinoma. During anatomical resection of the lung, tumor tissue and macroscopically adjacent healthy lung parenchyma (control) that was 5 cm away from the tumor were obtained. The tissues were freshly frozen and stored at −20°C. The generation of ROMs was monitored using luminol- and lucigenin-enhanced chemiluminescence (CL) techniques. RESULTS: Both luminol (specific for (.)OH, H(2)O(2), and HOCl(-)) and lucigenin (selective for O(2)(.-)) CL measurements were significantly higher in tumor tissues than in control tissues (P <0.001). Luminol and lucigenin CL measurements were 1.93 ± 0.71 and 2.5 ± 0.84 times brighter, respectively, in tumor tissues than in the adjacent parenchyma (P = 0.07). CONCLUSION: In patients with lung cancer, all ROM levels were increased in tumor tissues when compared with the adjacent lung tissue. Because the increase in lucigenin concentration, which is due to tissue ischemia, is higher than the increase in luminol, which is directly related to the presence and severity of inflammation, ischemia may be more important than inflammation for tumor development in patients with lung cancer

Renal complications of lipodystrophy: A closer look at the natural history of kidney disease

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/144612/1/cen13732_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/144612/2/cen13732.pd

INEQUALITIES AND EXPONENTIAL DECAY OF CERTAIN DIFFERENTIAL EQUATIONS OF FIRST ORDER IN TIME VARYING DELAY

WOS: 000401400600010In this paper, we give sufficient conditions to guarantee exponential decay of solutions to zero of the time varying delay differential equation of first order. By using the Lyapunov-Krasovskii functional approach, we establish new results on the exponential decay of solutions, which include and improve some related results in the literature

On Some Gualitative Behaviors of Certain Differential Equations of Fourth Order With Multiple Retardations

In this paper, we give sufficient conditions to guarantee the asymptotic stability and boundedness of solutions to a kind of fourth-order functional differential equations with multiple retardations. By using the Lyapunov-Krasovskii functional approach, we establish two new results on the stability and boundedness of solutions, which include and improve some related results in the literature. © 2016, Wilmington Scientific Publisher. All Rights Reserved

Boundedness and square integrability of solutions of nonlinear fourth-order differential equations with bounded delay

In this article, we give sufficient conditions for the boundedness, uniformly asymptotic stability and square integrability of the solutions to a fourth-order non-autonomous differential equation with bounded delay by using Lyapunov's second method

On the Qualitative Analysis of Volterra IDDEs with Infinite Delay

WOS:000539014600025This investigation deals with a nonlinear Volterra integro-differential equation with infinite retardation (IDDE). We will prove three new results on the stability, uniformly stability (US) and square integrability (SI) of solutions of that IDDE. The proofs of theorems rely on the use of an appropriate Lyapunov-Krasovskii functional (LKF). By the outcomes of this paper, we generalize and obtain some former results in mathematical literature under weaker conditions

Phenotypic correlations in a patient with ring chromosome 22

Ring chromosome 22, a rare cytogenetic anomaly, has been described in over 60 cases in the medical literature. The aim of this report was to present a case carrying ring chromosome 22, and her family

Investigation of the antioxidant effects of pheniramine maleate and nebivolol on testicular damage in rats with experimentally induced testis torsion

KISA, Ucler/0000-0002-8131-6810WOS: 000426974600004PubMed: 29513811Purpose: To investigate the biochemical, histopathologic, and spermatogenetic changes in the detorsionated testicle after experimental torsion and to study the antioxidant effects of pheniramine maleate and nebivolol. Methods: Twenty-four Sprague-Dawley male rats were divided into 4 groups: Group 1: Sham; Group 2: Torsion/Detorsion (T/D); Group 3: T/D + Pheniramine maleate (PM); Group 4: T/D + Nebivolol (NB) group. Paroxanase (PON), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stres index (OSI) were measured, and spermatogenetic and histopathologic evaluation was performed in tissue and blood samples. Results: The evaluation of tissue TAS indicated no statistically significant difference in Group 3 compared to Group 2. A statistically significant increase was detected in Group 4 compared to Group 2. Serum PON levels revealed a statistically significant increase in Groups 3 and 4 compared to Groups 1 and 2. The Johnsen testicular biopsy score decreased in Groups 3 and 4, but the decrease was not statistically significant. Conclusions: Pheniramine maleate and nebivolol have antioxidant effects against ischemia-reperfusion damage. They also support tissue recovery, which is more significantly observed by nebivolol

International Evaluation of Unrecognizably Uglifying Human Faces in Late and Severe Secondary Hyperparathyroidism in Chronic Kidney Disease. Sagliker Syndrome. A Unique Catastrophic Entity, Cytogenetic Studies for Chromosomal Abnormalities, Calcium-Sensing Receptor Gene and GNAS1 Mutations. Striking and Promising Missense Mutations on the GNAS1 Gene Exons 1, 4, 10, 4

WOS: 000298638800030PubMed ID: 22200434Hypotheses explaining pathogenesis of secondary hyperparathyroidism (SH) in late and severe CKD as a unique entity called Sagliker syndrome (SS) are still unclear. This international study contains 60 patients from Turkey, India, Malaysia, China, Romania, Egypt, Tunisia, Taiwan, Mexico, Algeria, Poland, Russia, and Iran. We examined patients and first degree relatives for cytogenetic chromosomal abnormalities, calcium sensing receptor (Ca SR) genes in exons 2 and 3 abnormalities and GNAS1 genes mutations in exons 1, 4, 5, 7, 10, 13. Our syndrome could be a new syndrome in between SH, CKD, and hereditary bone dystrophies. We could not find chromosomal abnormalities in cytogenetics and on Ca SR gene exons 2 and 3. Interestingly, we did find promising missense mutations on the GNAS1 gene exons 1, 4, 10, 4. We finally thought that those catastrophic bone diseases were severe SH and its late treatments due to monetary deficiencies and iatrogenic mistreatments not started as early as possible. This was a sine qua non humanity task. Those brand new striking GNAS1 genes missense mutations have to be considered from now on for the genesis of SS. (C) 2012 by the National Kidney Foundation, Inc. All rights reserved.Cukurova University, Adana, Turkey under Cukurova UniversityCukurova University [TF2009BAP8, TF2009BAP43, TF2009BAP21, TF2009D2]; ISURAT(International Society on Uremia Research and Toxicity)This study was mostly sponsored by Y.S. and by Cukurova University, Adana, Turkey for genetical material supplements under Cukurova University grants TF2009BAP8, TF2009BAP43, TF2009BAP21, and TF2009D2. Y.S. also participated to the ISURAT Congress, May 12-14, 2001, Nagoya, Japan as a speaker on this subject; his flight was sponsored by ISURAT(International Society on Uremia Research and Toxicity)