Development of value-added fishery products

Studies on the development of value-added products using arrow squid (Loligo sp.) and bullet tuna (Auxis rocheii, R.) were conducted. The recommended steps in new product development were systematically followed while establishing control to all steps in these product development studies to ensure that the requirements for quality fishery products are met. New product concepts were generated, evaluated, and refined. The most plausible concepts were then advanced to the product optimization stage wherein samples were subjected to a series of sensory evaluations to come up with the standardized formulations. Three (3) squid products, namely squid nuggets, squid loaf, and squid rolls, and two (2) bullet tuna products, namely tuna loaf and dried seasoned tuna, were then subjected to either accelerated or actual shelf life testings, consumer acceptability testings, and cost analyses. In collaboration with a private company, the most promising technologies generated from the preceding activities were advanced to pilot-scale production in which the HACCP-concept of quality assurance was applied, and the hazards and critical control points associated with each step in the production of the new products were established. The resulting products were finally subjected to consumer testing at the Japan Food Expo '95 which proved their acceptability to the export market as well as the products' feasibility for commercialization

Validation of the HPV Impact Profile in Portuguese women with human papilloma virus

Objective: Human papilloma virus (HPV) is the world's most common causative agent in sexually transmitted viral infections; thus requiring clinical guidance, before and after treatment, to promote better quality of life in women diagnosed with HPV. The HPV Impact Profile (HIP) is a comprehensive instrument used to assess the psychosocial impact of HPV and related interventions in a wide range of domains. The main purpose of this study was to validate the HIP in Portuguese women, so that it can be used in this population, both in research and in clinical settings. Methods: A sample of 178 Portuguese women infected with HPV were assessed with the following instruments: Index of Sexual Satisfaction, Hospital Anxiety and Depression Scale and HPV Impact Profile. Results: The results of the confirmatory factor analysis showed some changes to the original model, which resulted in an adapted version with six domains, compared to the seven domains of the original model. The final model showed a good fit to the data. All Cronbach's alphas were higher than 0.79 indicating a high level of internal consistency of the subscales. As in the original HIP questionnaire, the exception was sexual impact, with a lower, but acceptable, Cronbach's alpha. Convergent and discriminant validities of the instrument were also assessed. Conclusions: The Portuguese version of the HIP presents good psychometric properties, allowing its use in clinical trials as well as in clinical practice in order to evaluate quality of life in women with HPV.This study was supported by the Portuguese Foundation for Science and Technology and the Portuguese Ministry of Science, Technology and Higher Education through national funds and co-financed by FEDER through COMPETE2020 under the PT2020 Partnership Agreement [POCI-01-0145-FEDER-007653]

Folic acid-mesoporous silicon nanoparticles enhance the anticancer activity of the p73-activating small molecule LEM2

Many drugs with anticancer potential fail in their translation to the clinics due to problems related to pharmacokinetics. LEM2 is a new dual inhibitor of MDM2/mutp53-TAp73 interactions with interesting in vitro anticancer activity, which opens new hopes as an unconventional anticancer therapeutic strategy against cancers lacking p53 or with impaired p53 pathways. As others xanthone derivatives, LEM2 has limited aqueous solubility, posing problems to pursue in vivo assays, and therefore limiting its potential clinical translation. In this work, a mesopomus silicon (PSi)-based nanodelivery system was developed with folate functionalization (APTES-TCPSi-PEG-FA) for targeted delivery, which successfully increased LEM2 solubility when compared to bulk LEM2, evidenced in payload release study. Such effect was reflected on the increase of LEM2 cytotoxicity in HCT116 and MDA-MB-231 cancer cells when treated with LEM2-loaded APTES-TCPSi-PEG-FA, by reducing cell viability lower than 50% in comparison with bulk LEM2. Despite the reduced LEM2 loading degree, which still limits its application in further in vivo assays, the results obtained herein recognize PSi-based nanodelivery systems as a promising strategy to improve LEM2 anticancer activity and bioavailability, which will be relevant for the potential use of this potent TAp73 activator in anticancer therapy.Peer reviewe

Cannabinoids reduce ErbB2-driven breast cancer progression through Akt inhibition.

RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.BACKGROUND: ErbB2-positive breast cancer is characterized by highly aggressive phenotypes and reduced responsiveness to standard therapies. Although specific ErbB2-targeted therapies have been designed, only a small percentage of patients respond to these treatments and most of them eventually relapse. The existence of this population of particularly aggressive and non-responding or relapsing patients urges the search for novel therapies. The purpose of this study was to determine whether cannabinoids might constitute a new therapeutic tool for the treatment of ErbB2-positive breast tumors. We analyzed their antitumor potential in a well established and clinically relevant model of ErbB2-driven metastatic breast cancer: the MMTV-neu mouse. We also analyzed the expression of cannabinoid targets in a series of 87 human breast tumors. RESULTS: Our results show that both Delta9-tetrahydrocannabinol, the most abundant and potent cannabinoid in marijuana, and JWH-133, a non-psychotropic CB2 receptor-selective agonist, reduce tumor growth, tumor number, and the amount/severity of lung metastases in MMTV-neu mice. Histological analyses of the tumors revealed that cannabinoids inhibit cancer cell proliferation, induce cancer cell apoptosis, and impair tumor angiogenesis. Cannabinoid antitumoral action relies, at least partially, on the inhibition of the pro-tumorigenic Akt pathway. We also found that 91% of ErbB2-positive tumors express the non-psychotropic cannabinoid receptor CB2. CONCLUSIONS: Taken together, these results provide a strong preclinical evidence for the use of cannabinoid-based therapies for the management of ErbB2-positive breast cancer

Antifouling Marine Coatings with a Potentially Safer and Sustainable Synthetic Polyphenolic Derivative

The development of harmless substances to replace biocide-based coatings used to prevent or manage marine biofouling and its unwanted consequences is urgent. The formation of biofilms on submerged marine surfaces is one of the first steps in the marine biofouling process, which facilitates the further settlement of macrofoulers. Anti-biofilm properties of a synthetic polyphenolic compound, with previously described anti-settlement activity against macrofoulers, were explored in this work. In solution this new compound was able to prevent biofilm formation and reduce a pre-formed biofilm produced by the marine bacterium, Pseudoalteromonas tunicata. Then, this compound was applied to a marine coating and the formation of P. tunicata biofilms was assessed under hydrodynamic conditions to mimic the marine environment. For this purpose, polyurethane (PU)-based coating formulations containing 1 and 2 wt.% of the compound were prepared based on a prior developed methodology. The most effective formulation in reducing the biofilm cell number, biovolume, and thickness was the PU-based coating containing an aziridine-based crosslinker and 2 wt.% of the compound. To assess the marine ecotoxicity impact of this compound, its potential to disrupt endocrine processes was evaluated through the modulation of two nuclear receptors (NRs), peroxisome proliferator-activated receptor gamma (PPAR gamma), and pregnane X receptor (PXR). Transcriptional activation of the selected NRs upon exposure to the polyphenolic compound (10 mu M) was not observed, thus highlighting the eco-friendliness towards the addressed NRs of this new dual-acting anti-macro- and anti-microfouling agent towards the addressed NRs

REPENSANDO A AVALIAÇÃO DA APRENDIZAGEM

RESUMO: Este trabalho busca conhecer e compreender como o Curso de Graduação em Enfermagem da Universidade Federal de Santa Catarina (UFSC), avalia seus alunos. Analisa como a avaliação está estruturada nos planos de ensino de oito disciplinas do período profissionalizante. A partir da análise realizada percebe-se que as disciplinas utilizam como critério as notas, e todas atribuem pesos diferenciados para cada atividade, de acordo com critérios estabelecidos somente pelos professores. Assim, pode-se dizer que a prática “dita” como avaliação da aprendizagem, não passa de uma verificação. Neste sentido, as autoras apontam alguns caminhos que possam redirecionar esta prática. PALAVRAS CHAVES: Avaliação; Ensino-Aprendizagem; Graduação; Enfermagem

Computational approaches to Explainable Artificial Intelligence: Advances in theory, applications and trends

Financiado para publicación en acceso aberto: Universidad de Granada / CBUA.[Abstract]: Deep Learning (DL), a groundbreaking branch of Machine Learning (ML), has emerged as a driving force in both theoretical and applied Artificial Intelligence (AI). DL algorithms, rooted in complex and non-linear artificial neural systems, excel at extracting high-level features from data. DL has demonstrated human-level performance in real-world tasks, including clinical diagnostics, and has unlocked solutions to previously intractable problems in virtual agent design, robotics, genomics, neuroimaging, computer vision, and industrial automation. In this paper, the most relevant advances from the last few years in Artificial Intelligence (AI) and several applications to neuroscience, neuroimaging, computer vision, and robotics are presented, reviewed and discussed. In this way, we summarize the state-of-the-art in AI methods, models and applications within a collection of works presented at the 9th International Conference on the Interplay between Natural and Artificial Computation (IWINAC). The works presented in this paper are excellent examples of new scientific discoveries made in laboratories that have successfully transitioned to real-life applications.Funding for open access charge: Universidad de Granada / CBUA. The work reported here has been partially funded by many public and private bodies: by the MCIN/AEI/10.13039/501100011033/ and FEDER “Una manera de hacer Europa” under the RTI2018-098913-B100 project, by the Consejeria de Economia, Innovacion, Ciencia y Empleo (Junta de Andalucia) and FEDER under CV20-45250, A-TIC-080-UGR18, B-TIC-586-UGR20 and P20-00525 projects, and by the Ministerio de Universidades under the FPU18/04902 grant given to C. Jimenez-Mesa, the Margarita-Salas grant to J.E. Arco, and the Juan de la Cierva grant to D. Castillo-Barnes. This work was supported by projects PGC2018-098813-B-C32 & RTI2018-098913-B100 (Spanish “Ministerio de Ciencia, Innovacón y Universidades”), P18-RT-1624, UMA20-FEDERJA-086, CV20-45250, A-TIC-080-UGR18 and P20 00525 (Consejería de econnomía y conocimiento, Junta de Andalucía) and by European Regional Development Funds (ERDF). M.A. Formoso work was supported by Grant PRE2019-087350 funded by MCIN/AEI/10.13039/501100011033 by “ESF Investing in your future”. Work of J.E. Arco was supported by Ministerio de Universidades, Gobierno de España through grant “Margarita Salas”. The work reported here has been partially funded by Grant PID2020-115220RB-C22 funded by MCIN/AEI/10.13039/501100011033 and, as appropriate, by “ERDF A way of making Europe”, by the “European Union” or by the “European Union NextGenerationEU/PRTR”. The work of Paulo Novais is financed by National Funds through the Portuguese funding agency, FCT - Fundaça̋o para a Ciência e a Tecnologia within project DSAIPA/AI/0099/2019. Ramiro Varela was supported by the Spanish State Agency for Research (AEI) grant PID2019-106263RB-I00. José Santos was supported by the Xunta de Galicia and the European Union (European Regional Development Fund - Galicia 2014–2020 Program), with grants CITIC (ED431G 2019/01), GPC ED431B 2022/33, and by the Spanish Ministry of Science and Innovation (project PID2020-116201GB-I00). The work reported here has been partially funded by Project Fondecyt 1201572 (ANID). The work reported here has been partially funded by Project Fondecyt 1201572 (ANID). In [247], the project has received funding by grant RTI2018-098969-B-100 from the Spanish Ministerio de Ciencia Innovación y Universidades and by grant PROMETEO/2019/119 from the Generalitat Valenciana (Spain). In [248], the research work has been partially supported by the National Science Fund of Bulgaria (scientific project “Digital Accessibility for People with Special Needs: Methodology, Conceptual Models and Innovative Ecosystems”), Grant Number KP-06-N42/4, 08.12.2020; EC for project CybSPEED, 777720, H2020-MSCA-RISE-2017 and OP Science and Education for Smart Growth (2014–2020) for project Competence Center “Intelligent mechatronic, eco- and energy saving sytems and technologies”BG05M2OP001-1.002-0023. The work reported here has been partially funded by the support of MICIN project PID2020-116346GB-I00. The work reported here has been partially funded by many public and private bodies: by MCIN/AEI/10.13039/501100011033 and “ERDF A way to make Europe” under the PID2020-115220RB-C21 and EQC2019-006063-P projects; by MCIN/AEI/10.13039/501100011033 and “ESF Investing in your future” under FPU16/03740 grant; by the CIBERSAM of the Instituto de Salud Carlos III; by MinCiencias project 1222-852-69927, contract 495-2020. The work is partially supported by the Autonomous Government of Andalusia (Spain) under project UMA18-FEDERJA-084, project name Detection of anomalous behavior agents by DL in low-cost video surveillance intelligent systems. Authors gratefully acknowledge the support of NVIDIA Corporation with the donation of a RTX A6000 48 Gb. This work was conducted in the context of the Horizon Europe project PRE-ACT, and it has received funding through the European Commission Horizon Europe Program (Grant Agreement number: 101057746). In addition, this work was supported by the Swiss State Secretariat for Education, Research and Innovation (SERI) under contract nummber 22 00058. S.B Cho was supported by Institute of Information & Communications Technology Planning & Evaluation (IITP) grant funded by the Korean government (MSIT) (No. 2020-0-01361, Artificial Intelligence Graduate School Program (Yonsei University)).Junta de Andalucía; CV20-45250Junta de Andalucía; A-TIC-080-UGR18Junta de Andalucía; B-TIC-586-UGR20Junta de Andalucía; P20-00525Junta de Andalucía; P18-RT-1624Junta de Andalucía; UMA20-FEDERJA-086Portugal. Fundação para a Ciência e a Tecnologia; DSAIPA/AI/0099/2019Xunta de Galicia; ED431G 2019/01Xunta de Galicia; GPC ED431B 2022/33Chile. Agencia Nacional de Investigación y Desarrollo; 1201572Generalitat Valenciana; PROMETEO/2019/119Bulgarian National Science Fund; KP-06-N42/4Bulgaria. Operational Programme Science and Education for Smart Growth; BG05M2OP001-1.002-0023Colombia. Ministerio de Ciencia, Tecnología e Innovación; 1222-852-69927Junta de Andalucía; UMA18-FEDERJA-084Suíza. State Secretariat for Education, Research and Innovation; 22 00058Institute of Information & Communications Technology Planning & Evaluation (Corea del Sur); 2020-0-0136

CCR5 Expression Influences the Progression of Human Breast Cancer in a p53-dependent Manner

Chemokines are implicated in tumor pathogenesis, although it is unclear whether they affect human cancer progression positively or negatively. We found that activation of the chemokine receptor CCR5 regulates p53 transcriptional activity in breast cancer cells through pertussis toxin–, JAK2-, and p38 mitogen–activated protein kinase–dependent mechanisms. CCR5 blockade significantly enhanced proliferation of xenografts from tumor cells bearing wild-type p53, but did not affect proliferation of tumor xenografts bearing a p53 mutation. In parallel, data obtained in a primary breast cancer clinical series showed that disease-free survival was shorter in individuals bearing the CCR5Δ32 allele than in CCR5 wild-type patients, but only for those whose tumors expressed wild-type p53. These findings suggest that CCR5 activity influences human breast cancer progression in a p53-dependent manner

Moderate Consumption of Beer (with and without Ethanol) and Menopausal Symptoms: Results from a Parallel Clinical Trial in Postmenopausal Women

The menopausal transition can be a challenging period for women's health and a trigger of uncomfortable symptoms. Beer is the main food source of isoxanthohumol, a precursor of 8-prenylnaringenin, the strongest phytoestrogen identified to date. As phytoestrogens are reported to reduce perimenopausal symptoms, we evaluated if a daily moderate consumption of beer with (AB) and without alcohol (NAB) could improve menopausal symptoms and modify cardiovascular risk factors. A total of 37 postmenopausal women were enrolled in a parallel controlled intervention trial and assigned to three study groups: 16 were administered AB (330 mL/day), 7 NAB (660 mL/day), and 14 were in the control group. After a 6-month follow-up of the 34 participants who finished the trial, both interventions (AB and NAB) significantly reduced the severity of the menopause-related symptoms (p-value AB vs. Control: 0.009; p-value NAB vs. Control: 0.033). Moreover, AB had a beneficial net effect on psychological menopausal discomforts compared to the control group. As the sex hormone profile did not differ significantly between the study groups, the effects of both types of beers (AB and NAB) are attributed to the non-alcoholic fraction of beer. Furthermore, moderate NAB consumption improved the lipid profile and decreased blood pressure in postmenopausal women. View Full-Text Keywords: phytoestrogens; prenylflavonoids; polyphenols; health; menopause; alcohol; cardiovascular risk factor

Maternidade e adolescência: sentimentos e atitudes

Em um estudo de 96 puérperas adolescentes, observamos os sentimentos e atitudes das mesmas com relação ao futuro, no que se refere ao trabalho e à escola. Foram também avaliados os problemas ocorridos com as adolescentes quanto à aceitação delas pelo seu meio social. O relacionamento com o pai da criança também foi objeto do estudo, assim como quais as expectativas das mesmas frente ao atendimento recebido através dos profissionais da área da Saúde