Typologie des eaux de surface du bassin du Sebou par multi-approche : corrélation entre indice biologique global des réseaux de contrôle et de surveillance (IBG-RCS) et l’approche physicochimique et microbiologique

L’étude consiste en l’utilisation de la démarche biologique, particulièrement les indices IBGN et IBG-RCS, pour montrer l’évolution de la qualité biologique des eaux de surface du bassin du Sebou dans la période 2009-2011. La caractérisation physicochimique/microbiologique de la qualité des eaux de surface et la caractérisation biologique moyennant l’indice biocénotique IBG-RCS de l’an 2011ont fait l’objet d’un traitement statistique. Les résultats obtenus montrent une certaine amélioration dans la qualité des eaux de surface du bassin d’étude. L’analyse statistique a montré la formation de deux groupes corrélés négativement, le premier composé de l’oxygène dissous et l’indice IBG-RCS alors que le deuxième est formé des paramètres révélateurs de pollution organique (DBO5, DCO, NH4+, Phosphate Total PT et coliformes fécaux : CF).Mots-clés : Bassin Hydraulique du Sebou, indice IBG-RCS, qualité - biologique, macro-invertébrés, qualité physicochimique.Sebou’s water surface multi-approaches typology: correlation between the global biological index of control and surveillance networks (IBG-RCS) and the physicochemical/microbiological approachThe study consists of using the biological approach, especially the IBGN and the IBG-RCS indexes, to show the evolution of the biological surface waters quality of the Sebou basin in the period of 2009-2011. The physicochemical/microbiological surface waters quality characterization and the biological characterization (IBG-RCS index) of the year 2011 were the subject of a statistical processing. The results showed some improvement in surface waters quality. The statistical analysis showed the formation of two negatively correlated groups, the first consisting of dissolved oxygen and the IBG-RCS index while the second consists of the parameters indicative of pollution (BOD5, COD, NH4+, PT and CF).Keywords : hydraulic basin of Sebou, IBG-RCS index, biological quality, macroinvertebrates, physicochimical quality

Effects of sludge compost as an amendment on the morphological responses and on production parameters of radish and potato

The elimination of sewage sludge is one of the current environmental problems; their valorisation appears as a matter of organization and optimization of the techniques of their management or elimination can play the role of organic fertilizer because of their richness of organic matter and mineral compounds.This study examines the use of sludge compost elaborated as a fertilizer and compares it with compost without sludge, Manure (traditional input) and bare soil (no input). We studied the impact of these fertilizers on the soil (before and after cultivation), on the parameters of vegetative growth (morphological) and on the parameters of production. The application test was carried out on two crops: radishes and potatoes.The obtained results showed a significant increase in vegetative growth and production levels compared to bare soil (without input) and manure. For the physicochemical characterizations studied of the soil before and after the planting of the culture tested, the results show that the compost has an effect on soil

Analysis and modelling of tsunami-induced tilt for the 2007, M = 7.6, Tocopilla and the 2010, M = 8.8 Maule earthquakes, Chile, from long-base tiltmeter and broadband seismometer records

We present a detailed study of tsunami-induced tilt at in-land sites, to test the interest and feasibility of such analysis for tsunami detection and modelling. We studied tiltmeter and broadband seismometer records of northern Chile, detecting a clear s

Dietary soy and meat proteins induce distinct physiological and gene expression changes in rats

This study reports on a comprehensive comparison of the effects of soy and meat proteins given at the recommended level on physiological markers of metabolic syndrome and the hepatic transcriptome. Male rats were fed semi-synthetic diets for 1 wk that differed only regarding protein source, with casein serving as reference. Body weight gain and adipose tissue mass were significantly reduced by soy but not meat proteins. The insulin resistance index was improved by soy, and to a lesser extent by meat proteins. Liver triacylglycerol contents were reduced by both protein sources, which coincided with increased plasma triacylglycerol concentrations. Both soy and meat proteins changed plasma amino acid patterns. The expression of 1571 and 1369 genes were altered by soy and meat proteins respectively. Functional classification revealed that lipid, energy and amino acid metabolic pathways, as well as insulin signaling pathways were regulated differently by soy and meat proteins. Several transcriptional regulators, including NFE2L2, ATF4, Srebf1 and Rictor were identified as potential key upstream regulators. These results suggest that soy and meat proteins induce distinct physiological and gene expression responses in rats and provide novel evidence and suggestions for the health effects of different protein sources in human diets

Effect of nanosilica addition on the fresh properties and shrinkage of mortars with fly ash and superplasticizer

The ongoing use of various mineral additions along with chemical admixtures such as superplasticizers justifies the need for further research. Understanding and quantifying their effects and possible synergies on the fresh and hardened properties of cement-based materials is necessary, especially if some of these components are known to have a pozzolanic effect. This paper describes and models the fresh and hardened properties of cement mortars including nanosilica and fly ash, and relates their properties to the proportioning of these materials and the superplasticizer dosage. Mini-slump, Marsh cone and Lombardi cone tests were used to examine the properties of the fresh mortars, and to assess density, plastic shrinkage, and drying shrinkage up to 20 days. The equations presented in this paper make it possible to optimize mortar proportionings to the required levels of performance in both fresh and hardened states

Biallelic loss of function variants in PPP1R21 cause a neurodevelopmental syndrome with impaired endocytic function

Next‐generation sequencing (NGS) has been instrumental in solving the genetic basis of rare inherited diseases, especially neurodevelopmental syndromes. However, functional workup is essential for precise phenotype definition and to understand the underlying disease mechanisms. Using whole exome (WES) and whole genome sequencing (WGS) in four independent families with hypotonia, neurodevelopmental delay, facial dysmorphism, loss of white matter, and thinning of the corpus callosum, we identified four previously unreported homozygous truncating PPP1R21 alleles: c.347delT p.(Ile116Lysfs*25), c.2170_2171insGGTA p.(Ile724Argfs*8), c.1607dupT p.(Leu536Phefs*7), c.2063delA p.(Lys688Serfs*26) and found that PPP1R21 was absent in fibroblasts of an affected individual, supporting the allele's loss of function effect. PPP1R21 function had not been studied except that a large scale affinity proteomics approach suggested an interaction with PIBF1 defective in Joubert syndrome. Our co‐immunoprecipitation studies did not confirm this but in contrast defined the localization of PPP1R21 to the early endosome. Consistent with the subcellular expression pattern and the clinical phenotype exhibiting features of storage diseases, we found patient fibroblasts exhibited a delay in clearance of transferrin‐488 while uptake was normal. In summary, we delineate a novel neurodevelopmental syndrome caused by biallelic PPP1R21 loss of function variants, and suggest a role of PPP1R21 within the endosomal sorting process or endosome maturation pathway

Interepidemic Rift Valley Fever Virus Seropositivity, Northeastern Kenya

Exposure is associated with long-term retinal disease and is most common in rural settings among older men who have contact with aborting animals

The effects of phenoxodiol on the cell cycle of prostate cancer cell lines

Background: Prostate cancer is associated with a poor survival rate. The ability of cancer cells to evade apoptosis and exhibit limitless replication potential allows for progression of cancer from a benign to a metastatic phenotype. The aim of this study was to investigate in vitro the effect of the isoflavone phenoxodiol on the expression of cell cycle genes. Methods: Three prostate cancer cell lines-LNCaP, DU145, and PC3 were cultured in vitro, and then treated with phenoxodiol (10 μM and 30 μM) for 24 and 48 h. The expression of cell cycle genes p21WAF1, c-Myc, Cyclin-D1, and Ki-67 was investigated by Real Time PCR. Results: Here we report that phenoxodiol induces cell cycle arrest in the G1/S phase of the cell cycle, with the resultant arrest due to the upregulation of p21WAF1 in all the cell lines in response to treatment, indicating that activation of p21WAF1 and subsequent cell arrest was occurring via a p53 independent manner, with induction of cytotoxicity independent of caspase activation. We found that c-Myc and Cyclin-D1 expression was not consistently altered across all cell lines but Ki-67 signalling expression was decreased in line with the cell cycle arrest. Conclusions: Phenoxodiol demonstrates an ability in prostate cancer cells to induce significant cytotoxicity in cells by interacting with p21WAF1 and inducing cell cycle arrest irrespective of p53 status or caspase pathway interactions. These data indicate that phenoxodiol would be effective as a potential future treatment modality for both hormone sensitive and hormone refractory prostate cancer

Postepidemic Analysis of Rift Valley Fever Virus Transmission in Northeastern Kenya: A Village Cohort Study

RVFV infection causes significant disease in both human and animal populations, resulting in significant agricultural, economic and public health consequences. We conducted a cohort study on residents of a high-risk area to measure human anti-RVFV seroprevalence, to identify risk factors, and to estimate the durability of prior RVFV immunity. One hundred two individuals tested for RVFV exposure before the 2006–2007 RVF outbreak were restudied to determine interval anti-RVFV seroconversion and persistence of humoral immunity since 2006. Ninety-two additional subjects were enrolled from randomly selected households to help identify risk factors for current seropositivity. Seroprevalence in the region was high (23%). 1/85 at-risk individuals restudied in the follow-up cohort had seroconverted since early 2006. 29% of newly tested individuals were seropositive. After adjustment in multivariable logistic models, age, village, and drinking raw milk were significantly associated with RVFV seropositivity. Visual impairment (defined as ≤20/80) was much more likely in the RVFV-seropositive group. Among those with previous exposure, RVFV titers remained at protective levels (>1∶40) for more than 3 years. This study highlights the high seroprevalence among Northeastern Kenyans and the ongoing surge in seroprevalence with each RVF outbreak