Positive Schnellschnittränder sind mit einer erhöhten Wahrscheinlichkeit eines Lokalrezidivs beim R0-resezierten Kopf-Hals-Plattenepithelkarzinom assoziiert

Diese Dissertation beschäftigt sich mit dem operativen Verfahren bei Kopf-Hals-Plattenepithelkarzinomen in Bezug zu deren Rezidivrat

The relation between oxidative stress and adhesion molecules in Egyptian children and adolescents with type 1 diabetes mellitus

Background: Antioxidant potential decreases while plasma lipid peroxidation products increase in type1 diabetes mellitus. The vascular endothelium is a major target of oxidative stress (OS). Reactive oxygen species signal events leading to impairment of endothelial function and promotion of leukocyte adhesion to the vascular endothelium.. Objective: To explore the relation between OS and adhesion molecules in type1 diabetes and correlate it with the state of metabolic control, disease duration and microvascular complications (MVCs). Design: Thirty-eight type 1 diabetics were included: 22 patients with disease duration less than 5 years and 16 patients with duration of 5 years or more. Thirty healthy age and sex matched subjects served as controls. They were assessed clinically. Laboratory investigations included, random blood sugar (RBS), glycated hemoglobin (HbA1c), fasting lipid profile and measurement of serum malondialdehyde (MDA) as a marker of lipid peroxidation and serum soluble P-selectin as a marker of endothelial/platelet activation. Results: Serum MDA and P-selectin were significantly elevated in type 1 diabetics compared to controls with the highest level in diabetics with disease duration of 5 years or more (p <0.0001). Both MDA and P-selectin levels were significantly elevated in complicated compared to non complicated diabetics (P < 0.0001) with strong relation to complication severity. Serum MDA level was positively correlated with serum P-selectin level in diabetics (p 0.0001). Serum MDA and P-selectin were positively and significantly correlated with disease duration (p < 0.0001 ), RBS (p < 0.0001,p=0.001 respectively), HbA1c (p < 0.0001), diastolic blood pressure (p=0.03,p=0.005 respectively), total cholesterol (p=0.04,p=0.02, respectively), triglycerides (p=0.006, p < 0.0001 respectively) and low density lipoproteins (p=0.03,p=0.05 respectively) but negatively correlated with high density lipoproteins(p=0.03). On multiple regression analysis, HbA1c had the strongest effect on both MDA and P-selectin levels (P <0.0001). Cut off values for serum MDA and Pselectin equal to 8.035 nmoles/ml and 45.15ng/dl respectively for early detection of diabetic MVCs were defined. Conclusion: Levels of MAD and P-selectin are elevated in type1 diabetics with evident relation to disease duration, metabolic control and severity of MVCs. Hence both of them might act as good markers to identify diabetics who are more susceptible to develop vascular disease.Keywords: Type1 diabetes, oxidative stress, P-selectin, adhesion molecules, microvascular complicationsEgypt J Pediatr Allergy Immunol 2009; 7(2): 65-7

SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM CALLUS AND SUSPENSION CULTURES OF IPHIONA MUCRONATA (FORSSK)

A protocol was designed for plant regeneration of Iphiona mucronata from embryogenic callus via somatic embryogenesis to enable micro propagation of this endangered plant. The embryogenic callus was induced using seedling cultured for nine months on Murashig and Skoog medium (MS) supplemented with 0.1 mg l-1 naphthalene acetic acid (NAA), 0.1 mg l-1 kinetin (Kn) and 5 mg l-1 ascorbic acid and incubated in the dark followed by growing on hormone free medium. Transfer of developed embryos to MS medium supplemented with 0.5 mg l-1 kinetin induced shoot formation. Four treatments were further tried for plant development by using indole acetic acid (IAA) or indole butyric acid (IBA) alone or in combination with kinetin. The results showed that 2 mg l-1 IAA was the best for in vitro plantlet regeneration. Embryogenic suspension was induced by transfer of embryogenic callus to liquid medium having the same composition followed by hormone free medium where different stages of embryos were monitored. Shoots were developed upon transfer to liquid medium supplemented with 0.5 mg l-1 Kn. However, no further development appeared upon transfer to semi solid medium containing different phytohormones. Embryogenic callus showed the highest phenolic contents when compared with embryogenic suspension, regenerated plantlets and the parent plant while flavonoids were detected only in embryogenic callus

Case report 476

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46788/1/256_2004_Article_BF00351012.pd

Influence of the chirality of short peptide supramolecular hydrogels in protein crystallogenesis

For the first time the influence of the chirality of the gel fibers in protein crystallogenesis has been studied. Enantiomeric hydrogels 1 and 2 were tested with model proteins lysozyme and glucose isomerase and a formamidase from B. cereus. Crystallization behaviour and crystal quality of these proteins in both hydrogels are presented and compared.MICINN (Spain) projects BIO2010-16800 (JAG), CTQ-2011.22455 (LAC & JMC), CTQ2012-34778 (JJDM & ALG), “Factoría Española de Cristalización” Consolider-Ingenio 2010 (JAG & MCM) and EDRF Funds (JAG, LAC & JMC), P12-FQM-2721 (LAC) Junta de Andalucía.MINECO,Project No. FIS2013-41821-R