Beyond Never-Never Land: Integrating LiDAR and Geophysical Surveys at the Johnston Site, Pinson Mounds State Archaeological Park, Tennessee, USA

Archaeologists often use near-surface geophysics or LiDAR-derived topographic imagery in their research. However, rarely are the two integrated in a way that offers a robust understanding of the complex historical palimpsests embedded within a social landscape. In this paper we present an integrated aerial and terrestrial remote sensing program at the Johnston Site, part of the larger Pinson Mounds landscape in the American MidSouth. Our work at Johnston was focused on better understanding the history of human landscape use and change so that we can begin to compare the Johnston Site with other large Middle Woodland (200 BC-AD 500) ceremonial centers in the region. Our research allowed us to examine the accuracy of an early map of the Johnston Site made in the early 20th century. However, our integrated remote sensing approach allows us to go well beyond testing the usefulness of the map; it helps identify different uses of the site through time and across space. Our research emphasizes the importance of an integrated remote sensing methodology when examining complex social landscapes of the past and present

Beyond Never-Never Land: Integrating LiDAR and Geophysical Surveys at the Johnston Site, Pinson Mounds State Archaeological Park, Tennessee, USA

Archaeologists often use near-surface geophysics or LiDAR-derived topographic imagery in their research. However, rarely are the two integrated in a way that offers a robust understanding of the complex historical palimpsests embedded within a social landscape. In this paper we present an integrated aerial and terrestrial remote sensing program at the Johnston Site, part of the larger Pinson Mounds landscape in the American MidSouth. Our work at Johnston was focused on better understanding the history of human landscape use and change so that we can begin to compare the Johnston Site with other large Middle Woodland (200 BC-AD 500) ceremonial centers in the region. Our research allowed us to examine the accuracy of an early map of the Johnston Site made in the early 20th century. However, our integrated remote sensing approach allows us to go well beyond testing the usefulness of the map; it helps identify different uses of the site through time and across space. Our research emphasizes the importance of an integrated remote sensing methodology when examining complex social landscapes of the past and present

The role of CXCL10 in the pathogenesis of experimental septic shock

Introduction: The chemokine CXCL10 is produced during infection and inflammation to activate the chemokine receptor CXCR3, an important regulator of lymphocyte trafficking and activation. The goal of this study was to assess the contributions of CXCL10 to the pathogenesis of experimental septic shock in mice. Methods: Septic shock was induced by cecal ligation and puncture (CLP) in mice resuscitated with lactated Ringer's solution and, in some cases, the broad spectrum antibiotic Primaxin. Studies were performed in CXCL10 knockout mice and mice treated with anti-CXCL10 immunoglobulin G (IgG). Endpoints included leukocyte trafficking and activation, core body temperature, plasma cytokine concentrations, bacterial clearance and survival. Results: CXCL10 was present at high concentrations in plasma and peritoneal cavity during CLP-induced septic shock. Survival was significantly improved in CXCL10 knockout (CXCL10KO) mice and mice treated with anti-CXCL10 IgG compared to controls. CXCL10KO mice and mice treated with anti-CXCL10 IgG showed attenuated hypothermia, lower concentrations of interleukin-6 (IL-6) and macrophage inhibitory protein-2 (MIP-2) in plasma and lessened natural killer (NK) cell activation compared to control mice. Compared to control mice, bacterial burden in blood and lungs was lower in CXCL10-deficient mice but not in mice treated with anti-CXCL10 IgG. Treatment of mice with anti-CXCL10 IgG plus fluids and Primaxin at 2 or 6 hours after CLP significantly improved survival compared to mice treated with non-specific IgG under the same conditions. Conclusions: CXCL10 plays a role in the pathogenesis of CLP-induced septic shock and could serve as a therapeutic target during the acute phase of septic shock

The response of Tampa Bay to a legacy mining nutrient release in the year following the event

IntroductionCultural eutrophication threatens numerous ecological and economical resources of Florida’s coastal ecosystems, such as beaches, mangroves, and seagrasses. In April 2021, an infrastructure failure at the retired Piney Point phosphorus mining retention reservoir garnered national attention, as 814 million liters of nutrient rich water were released into Tampa Bay, Florida over 10 days. The release of nitrogen and phosphorus-rich water into Tampa Bay – a region that had been known as a restoration success story since the 1990s – has highlighted the potential for unexpected challenges for coastal nutrient management.MethodsFor a year after the release, we sampled bi-weekly at four sites to monitor changes in nutrients, stable isotopes, and phytoplankton communities, complemented with continuous monitoring by multiparameter sondes. Our data complement the synthesis efforts of regional partners, the Tampa Bay and Sarasota Bay Estuary Programs, to better understand the effects of anthropogenic nutrients on estuarine health.ResultsPhytoplankton community structure indicated an initial diatom bloom that dissipated by the end of April 2021. In the summer, the bay was dominated by Karenia brevis, with conditions improving into the fall. To determine if there was a unique carbon (C) and nitrogen (N) signature of the discharge water, stable isotope values of carbon (δ13C) and nitrogen (δ15N) were analyzed in suspended particulate material (SPM). The δ15N values of the discharge SPM were −17.88‰ ± 0.76, which is exceptionally low and was unique relative to other nutrient sources in the region. In May and early June of 2021, all sites exhibited a decline in the δ15N values of SPM, suggesting that discharged N was incorporated into SPM after the event. The occurrence of very low δ15N values at the reference site, on the Gulf Coast outside of the Bay, indicates that some of the discharge was transported outside of Tampa Bay.DiscussionThis work illustrates the need for comprehensive nutrient management strategies to assess and manage the full range of consequences associated with anthropogenic nutrient inputs into coastal ecosystems. Ongoing and anticipated impacts of climate change – such as increasing tropical storm intensity, temperatures, rainfall, and sea level rise – will exacerbate this need

New insights into the classification and nomenclature of cortical GABAergic interneurons.

A systematic classification and accepted nomenclature of neuron types is much needed but is currently lacking. This article describes a possible taxonomical solution for classifying GABAergic interneurons of the cerebral cortex based on a novel, web-based interactive system that allows experts to classify neurons with pre-determined criteria. Using Bayesian analysis and clustering algorithms on the resulting data, we investigated the suitability of several anatomical terms and neuron names for cortical GABAergic interneurons. Moreover, we show that supervised classification models could automatically categorize interneurons in agreement with experts' assignments. These results demonstrate a practical and objective approach to the naming, characterization and classification of neurons based on community consensus

Regional to global scale atmospheric effects of the emerging mainland Chinese transportation system

This is the final report of a one-year Laboratory Directed Research and Development (LDRD) project at Los Alamos National Laboratory (LANL). The People`s Republic of China will be moving toward considerably increased use of motorized vehicles over the next human generation. Estimates are for a fleet of 100 million vehicles or more. Large increases in ozone and other types of pollution associated with automobiles will result and this will have significant impact across Asia and into the Pacific. In this project the authors have taken a continental scale approach in a two-dimensional photochemical model to estimate the ozone concentrations to be expected. Potential levels of nitrate/soot in aerosols and increase in carbon dioxide are also discussed. Major pollution problems are forecast for the Pacific Rim if Chinese projections for their automotive sector are realized

Tree-Ring-Reconstructed Summer Temperatures from Northwestern North America during the Last Nine Centuries*

Northwestern North America has one of the highest rates of recent temperature increase in the world, but the putative “divergence problem” in dendroclimatology potentially limits the ability of tree-ring proxy data at high latitudes to provide long-term context for current anthropogenic change. Here, summer temperatures are reconstructed from a Picea glauca maximum latewood density (MXD) chronology that shows a stable relationship to regional temperatures and spans most of the last millennium at the Firth River in northeastern Alaska. The warmest epoch in the last nine centuries is estimated to have occurred during the late twentieth century, with average temperatures over the last 30 yr of the reconstruction developed for this study [1973–2002 in the Common Era (CE)] approximately 1.3° ± 0.4°C warmer than the long-term preindustrial mean (1100–1850 CE), a change associated with rapid increases in greenhouse gases. Prior to the late twentieth century, multidecadal temperature fluctuations covary broadly with changes in natural radiative forcing. The findings presented here emphasize that tree-ring proxies can provide reliable indicators of temperature variability even in a rapidly warming climate

Germline MBD4-deficiency causes a multi-tumor predisposition syndrome

We report an autosomal recessive, multi-organ tumor predisposition syndrome, caused by bi-allelic loss-of-function germline variants in the base excision repair (BER) gene MBD4. We identified five individuals with bi-allelic MBD4 variants within four families and these individuals had a personal and/or family history of adenomatous colorectal polyposis, acute myeloid leukemia, and uveal melanoma. MBD4 encodes a glycosylase involved in repair of G:T mismatches resulting from deamination of 5′-methylcytosine. The colorectal adenomas from MBD4-deficient individuals showed a mutator phenotype attributable to mutational signature SBS1, consistent with the function of MBD4. MBD4-deficient polyps harbored somatic mutations in similar driver genes to sporadic colorectal tumors, although AMER1 mutations were more common and KRAS mutations less frequent. Our findings expand the role of BER deficiencies in tumor predisposition. Inclusion of MBD4 in genetic testing for polyposis and multi-tumor phenotypes is warranted to improve disease management