Glassy Carbon Electrode Modified with Layering of Carbon Black/Poly(Allylamine Hydrochloride) Composite for Multianalyte Determination

© 2020 Wiley-VCH GmbH An electrochemical sensor using glassy carbon electrode modified with carbon black within a poly(allylamine hydrochloride) film is proposed in this work. The novel sensor was characterized by scanning electron microscopy, electrochemical impedance spectroscopy, and cyclic voltammetry using the redox probe Fe(CN)63−/4−. The sensor was applied for the simultaneous determination of dopamine (DA), paracetamol (PAR), amlodipine (AML), and rosuvastatin (RSV). The quantification of all four analytes was carried out by linear sweep voltammetry and presented a linear concentration range for all analytes from 1.0 to 90 μmol L−1, with limit of detection of 0.55, 1.3, 5.7, and 3.0 μmol L−1 for DA, PAR, AML, and RSV, respectively. This sensor was successfully applied in the simultaneous determination of these analytes in environmental, pharmaceutical, and biological samples

O lugar ocupado pela assistência fisioterapêutica: representações sociais de trabalhadores com DORT

INTRODUÇÃO: Os distúrbios osteomusculares relacionados ao trabalho (DORT) articulam-se diretamente à realização de atividades ocupacionais e às condições de trabalho. OBJETIVO: O artigo origina-se de uma pesquisa qualitativa que analisou as representações sociais da assistência fisioterapêutica entre trabalhadores portadores de Distúrbios Osteomusculares Relacionados ao Trabalho (DORT), frequentadores do Centro de Referência em Saúde do Trabalhador (CEREST), pelo Departamento de Saúde do Trabalhador (DSAT), Juiz de Fora (MG). MATERIAIS E MÉTODOS: O recurso de apreensão das informações foi a entrevista semiestruturada, realizada com 12 trabalhadoras com idades entre 29 e 55 anos. RESULTADOS: As informações submetidas à análise temática apontaram que a assistência fisioterapêutica recebida repercute em períodos longos de tratamento e na insatisfação dos resultados, por meio de uma prática curativista, segmentada e pouco resolutiva, na qual prevalece a utilização de equipamentos em detrimento das manobras corporais e holísticas. CONCLUSÃO: Diante dos relatos, verificamos a necessidade de uma atenção humanizada, integral e equânime com responsabilização e vínculo, além da valorização dos trabalhadores e usuários, promovendo melhor qualidade de vida. É imprescindível a realização de novos estudos que possibilitem melhor compreensão e aprofundamento teórico e prático da relação entre a atuação do fisioterapeuta e as representações sociais envolvidas

The evidence base for circulating tumour DNA blood-based biomarkers for the early detection of cancer: a systematic mapping review

Background: The presence of circulating cell-free DNA from tumours in blood (ctDNA) is of major importance to those interested in early cancer detection, as well as to those wishing to monitor tumour progression or diagnose the presence of activating mutations to guide treatment. In 2014, the UK Early Cancer Detection Consortium undertook a systematic mapping review of the literature to identify blood-based biomarkers with potential for the development of a non-invasive blood test for cancer screening, and which identified this as a major area of interest. This review builds on the mapping review to expand the ctDNA dataset to examine the best options for the detection of multiple cancer types. Methods: The original mapping review was based on comprehensive searches of the electronic databases Medline, Embase, CINAHL, the Cochrane library, and Biosis to obtain relevant literature on blood-based biomarkers for cancer detection in humans (PROSPERO no. CRD42014010827). The abstracts for each paper were reviewed to determine whether validation data were reported, and then examined in full. Publications concentrating on monitoring of disease burden or mutations were excluded. Results: The search identified 94 ctDNA studies meeting the criteria for review. All but 5 studies examined one cancer type, with breast, colorectal and lung cancers representing 60% of studies. The size and design of the studies varied widely. Controls were included in 77% of publications. The largest study included 640 patients, but the median study size was 65 cases and 35 controls, and the bulk of studies (71%) included less than 100 patients. Studies either estimated cfDNA levels non-specifically or tested for cancer-specific mutations or methylation changes (the majority using PCR-based methods). Conclusion: We have systematically reviewed ctDNA blood biomarkers for the early detection of cancer. Pre-analytical, analytical, and post-analytical considerations were identified which need to be addressed before such biomarkers enter clinical practice. The value of small studies with no comparison between methods, or even the inclusion of controls is highly questionable, and larger validation studies will be required before such methods can be considered for early cancer detection

The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence

Trost E, Ott L, Schneider J, et al. The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence. BMC Genomics. 2010;11(1): 728