Seasonal dynamics of dissolved organic matter in the Mackenzie Delta, Canadian Arctic waters

Increasing air temperatures and associated permafrost thaw in Arctic river watersheds, such as the Mackenzie River catchment, are directly affecting the aquatic environment. As a consequence, the quantity and the quality of dissolved organic carbon (DOC) that is transported via the Mackenzie River into the Arctic Ocean is expected to change. Particularly in these remote permafrost regions of the Arctic, monitoring of terrigenous organic carbon fluxes is insufficient and knowledge of distribution and fate of organic carbon when released to the coastal waters is remarkably lacking. Despite its poorly evaluated performance in Arctic coastal waters, Satellite Ocean Colour Remote Sensing (SOCRS) remains a powerful tool to complement monitoring of land-ocean DOC fluxes, detect their trends, and help in understanding their propagation in the Arctic Ocean. In this study, we use in situ and SOCRS data to show the strong seasonal dynamics of the Mackenzie River plume and the spatial distribution of associated terrigenous DOC on the Beaufort Sea Shelf for the first time. Using a dataset collected during an extensive field campaign in 2019, the performance of three commonly-used atmospheric correction (AC) algorithms and two available colored dissolved organic matter (CDOM) retrieval algorithms were evaluated using the Ocean and Land Colour Instrument (OLCI). Our results showed that in optically-complex Arctic coastal waters the Polymer AC algorithm performed the best. For the retrieval of CDOM, the gsmA algorithm (Mean Percentage Error (MPE) = 35.7%) showed slightly more consistent results compared to the ONNS algorithm (MPE = 37.9%). By merging our measurements with published datasets, the newly-established DOC-CDOM relationship for the Mackenzie-Beaufort Sea region allowed estimations of DOC concentrations from SOCRS across the entire fluvial-marine transition zone with an MPE of 20.5%. Finally, we applied SOCRS with data from the Sentinel-3 OLCI sensor to illustrate the seasonal variation of DOC concentrations in the surface waters of the Beaufort Sea on a large spatial scales and high frequency throughout the entire open water period. Highest DOC concentrations and largest lateral extent of the plume were observed in spring right after the Mackenzie River ice break-up indicating that the freshet was the main driver of plume propagation and DOC distribution on the shelf. Satellite-derived images of surface water DOC concentration placed the in situ observations into a larger temporal and spatial context and revealed a strong seasonal variability in transport pathways of DOC in the Mackenzie- Beaufort Sea region

Development of a Fully Automated Flow Injection Analyzer Implementing Bioluminescent Biosensors for Water Toxicity Assessment

This paper describes the development of an automated Flow Injection analyzer for water toxicity assessment. The analyzer is validated by assessing the toxicity of heavy metal (Pb2+, Hg2+ and Cu2+) solutions. One hundred μL of a Vibrio fischeri suspension are injected in a carrier solution containing different heavy metal concentrations. Biosensor cells are mixed with the toxic carrier solution in the mixing coil on the way to the detector. Response registered is % inhibition of biosensor bioluminescence due to heavy metal toxicity in comparison to that resulting by injecting the Vibrio fischeri suspension in deionised water. Carrier solutions of mercury showed higher toxicity than the other heavy metals, whereas all metals show concentration related levels of toxicity. The biosensor’s response to carrier solutions of different pHs was tested. Vibrio fischeri’s bioluminescence is promoted in the pH 5–10 range. Experiments indicate that the whole cell biosensor, as applied in the automated fluidic system, responds to various toxic solutions

Ice-rich (periglacial) vs icy (glacial) depressions in the Argyre region, Mars: a proposed cold-climate dichotomy of landforms

On Mars, so-called “scalloped depressions” are widely observed in Utopia Planitia (UP) and Malea Planum (MP). Typically, they are rimless, metres- to decametres-deep, incised sharply, tiered inwardly, polygonised and sometimes pitted. The depressions seemingly incise terrain that is icy and possibly thermokarstic, i.e. produced by the thermal destabilisation of the icy terrain. Agewise, the depressions are thought to be relatively youthful, originating in the Late Amazonian Epoch.Here, we report the presence of similar depressions in the Argyre region (AR) (30–60° S; 290–355° E). More importantly, we separate and differentiate these landforms into two groups: (ice-rich) periglacial depressions (Type-1); and, (icy) glacial depressions (Type-2a-c). This differentiation is presented to the Mars community for the first time.Based on a suite of morphological and geological characteristics synonymous with ice-complexes in the Lena Peninsula (eastern Russia) and the Tuktoyaktuk Coastlands (Northwest Territories, Canada), we propose that the Type-1 depressions are ice-rich periglacial basins that have undergone volatile depletion largely by sublimation and as the result of thermal destabilisation. In keeping with the terms and associated definitions derived of terrestrial periglacial-geomorphology, ice-rich refers to permanently frozen-ground in which ice lenses or segregation ice (collectively referenced as excess ice) have formed.We suggest that the depressions are the product of a multi-step, cold-climate geochronology:(1) Atmospheric precipitation and surface accumulation of an icy mantle during recent high obliquities.(2) Regional or local triple-point conditions and thaw/evaporation of the mantle, either by exogenic forcing, i.e. obliquity-driven rises of aerial and sub-aerial temperatures, or endogenic forcing, i.e. along Argyre impact-related basement structures.(3) Meltwater migration into the regolith, at least to the full depth of the depressions.(4) Freeze-thaw cycling and the formation of excess ice.(5) Sublimation of the excess ice and depression formation as high obliquity dissipates and near-surface ice becomes unstable.The Type-2 depressions exhibit characteristics suggestive of (supra-glacial) dead-ice basins and snow/ice suncups observed in high-alpine landscapes on Earth, e.g. the Swiss Alps and the Himalayas. Like the Type-1 depressions, the Type-2 depressions could be the work of sublimation; however, the latter differ from the former in that they seem to develop within a glacial-like icy mantle that blankets the surface rather than within an ice-rich and periglacially-revised regolith at/near the surface.Interestingly, the Type-2 depressions overlie the Type-1 depressions at some locations. If the periglacial/glacial morphological and stratigraphical dichotomy of depressions is valid, then this points to recent glaciation at some locations within the AR being precursed by at least one episode of periglaciation. This also suggests that periglaciation has a deeper history in the region than has been thought hitherto. Moreover, if the hypothesised differences amongst the Argyre-based depressions are mirrored in Utopia Planitia and Malea Planum, then perhaps this periglacial-glacial dichotomy and its associated geochronology are as relevant to understanding late period landscape-evolution in these two regions as it is in the AR

An ice-rich flow origin for the banded terrain in the Hellas basin, Mars

The interior of Hellas Basin displays a complex landscape and a variety of geomorphological domains. One of these domains, the enigmatic banded terrain covers much of the northwestern part of the basin. We use high-resolution (Context Camera and High-Resolution Imaging Science Experiment) Digital Terrain Models to show that most of the complex viscous flowing behavior exhibited by the banded terrain is controlled by topography and flow-like interactions between neighboring banded terrain. Furthermore, the interior of the basin hosts several landforms suggestive of the presence of near-surface ice, which include polygonal patterns with elongated pits, scalloped depressions, isolated mounds, and collapse structures. We suggest that thermal contraction cracking and sublimation of near-surface ice are responsible for the formation and the development of most of the ice-related landforms documented in Hellas. The relatively pristine form, lack of superposed craters, and strong association with the banded terrain, suggest an Amazonian (<3 Ga) age of formation for these landforms. Finally, relatively high surface pressures (above the triple point of water) expected in Hellas and summertime temperatures often exceeding the melting point of water ice suggest that the basin may have recorded relatively “temperate” climatic conditions compared to other places on Mars. Therefore, the potentially ice-rich banded terrain may have deformed with lower viscosity and stresses compared to other locations on Mars, which may account for its unique morphology

Exploring Powered Wheelchair Users and Their Caregivers’ Perspectives on Potential Intelligent Power Wheelchair Use: A Qualitative Study

Power wheelchairs (PWCs) can have a positive impact on user well-being, self-esteem, pain, activity and participation. Newly developed intelligent power wheelchairs (IPWs), allowing autonomous or collaboratively-controlled navigation, could enhance mobility of individuals not able to use, or having difficulty using, standard PWCs. The objective of this study was to explore the perspectives of PWC users (PWUs) and their caregivers regarding if and how IPWs could impact on current challenges faced by PWUs, as well as inform current development of IPWs. A qualitative exploratory study using individual interviews was conducted with PWUs (n = 12) and caregivers (n = 4). A semi-structured interview guide and video were used to facilitate informed discussion regarding IPWs. Thematic analysis revealed three main themes: (1) "challenging situations that may be overcome by an IPW" described how the IPW features of obstacle avoidance, path following, and target following could alleviate PWUs' identified mobility difficulties; (2) "cautious optimism concerning IPW use revealed participants" addresses concerns regarding using an IPW as well as technological suggestions; (3) "defining the potential IPW user" revealed characteristics of PWUs that would benefit from IPW use. Findings indicate how IPW use may help overcome PWC difficulties and confirm the importance of user input in the ongoing development of IPWs

Complex geomorphologic assemblage of terrains in association with the banded terrain in Hellas basin, Mars

Hellas basin acts as a major sink for the southern highlands of Mars and is likely to have recorded several episodes of sedimentation and erosion. The north-western part of the basin displays a potentially unique Amazonian landscape domain in the deepest part of Hellas, called “banded terrain”, which is a deposit characterized by an alternation of narrow band shapes and inter-bands displaying a sinuous and relatively smooth surface texture suggesting a viscous flow origin. Here we use high-resolution (HiRISE and CTX) images to assess the geomorphological interaction of the banded terrain with the surrounding geomorphologic domains in the NW interior of Hellas to gain a better understanding of the geological evolution of the region as a whole. Our analysis reveals that the banded terrain is associated with six geomorphologic domains: a central plateau named Alpheus Colles, plain deposits (P1 and P2), reticulate (RT1 and RT2) and honeycomb terrains. Based on the analysis of the geomorphology of these domains and their cross-cutting relationships, we show that no widespread deposition post-dates the formation of the banded terrain, which implies that this domain is the youngest and latest deposit of the interior of Hellas. Therefore, the level of geologic activity in the NW Hellas during the Amazonian appears to have been relatively low and restricted to modification of the landscape through mechanical weathering, aeolian and periglacial processes. Thermophysical data and cross-cutting relationships support hypotheses of modification of the honeycomb terrain via vertical rise of diapirs such as ice diapirism, and the formation of the plain deposits through deposition and remobilization of an ice-rich mantle deposit. Finally, the observed gradual transition between honeycomb and banded terrain suggests that the banded terrain may have covered a larger area of the NW interior of Hellas in the past than previously thought. This has implications on the understanding of the evolution of the deepest part of Hellas

MAP Kinase Phosphatase-2 Plays a Critical Role in Response to Infection by Leishmania mexicana

In this study we generated a novel dual specific phosphatase 4 (DUSP4) deletion mouse using a targeted deletion strategy in order to examine the role of MAP kinase phosphatase-2 (MKP-2) in immune responses. Lipopolysaccharide (LPS) induced a rapid, time and concentration-dependent increase in MKP-2 protein expression in bone marrow-derived macrophages from MKP-2+/+ but not from MKP-2−/− mice. LPS-induced JNK and p38 MAP kinase phosphorylation was significantly increased and prolonged in MKP-2−/− macrophages whilst ERK phosphorylation was unaffected. MKP-2 deletion also potentiated LPS-stimulated induction of the inflammatory cytokines, IL-6, IL-12p40, TNF-α, and also COX-2 derived PGE2 production. However surprisingly, in MKP-2−/− macrophages, there was a marked reduction in LPS or IFNγ-induced iNOS and nitric oxide release and enhanced basal expression of arginase-1, suggesting that MKP-2 may have an additional regulatory function significant in pathogen-mediated immunity. Indeed, following infection with the intracellular parasite Leishmania mexicana, MKP-2−/− mice displayed increased lesion size and parasite burden, and a significantly modified Th1/Th2 bias compared with wild-type counterparts. However, there was no intrinsic defect in MKP-2−/− T cell function as measured by anti-CD3 induced IFN-γ production. Rather, MKP-2−/− bone marrow-derived macrophages were found to be inherently more susceptible to infection with Leishmania mexicana, an effect reversed following treatment with the arginase inhibitor nor-NOHA. These findings show for the first time a role for MKP-2 in vivo and demonstrate that MKP-2 may be essential in orchestrating protection against intracellular infection at the level of the macrophage

Pesticide use and opportunities of exposure among farmers and their families: cross-sectional studies 1998-2006 from Hebron governorate, occupied Palestinian territory

<p>Abstract</p> <p>Background</p> <p>Adverse health effects caused by pesticide exposure have been reported in occupied Palestinian territory and the world at large. The objective of this paper is to compare patterns of pesticide use in Beit-U'mmar village, West Bank, between 1998 and 2006.</p> <p>Methods</p> <p>We studied two populations in Beit-U'mmar village, comprised of: 1) 61 male farmers and their wives in 1998 and 2) 250 male farmers in 2006. Both populations completed a structured interview, which included questions about socio-demographic factors, types of farming tasks, as well as compounds, quantities, and handling of pesticides. Using the 1998 population as a reference, we applied generalized linear regression models (GLM) and 95% confidence intervals (CI) in order to estimate prevalence differences (PD) between the two populations.</p> <p>Results</p> <p>In 1998, farmers used 47 formulated pesticides on their crops. In 2006, 16 of these pesticides were still in use, including five internationally banned compounds. There were positive changes with less use of large quantities of pesticides (>40 units/year) (PD -51; CI -0.60, -0.43), in applying the recommended dosage of pesticides (PD +0.57; CI +0.48, +0.68) and complying with the safety period (PD +0.89; CI+0.83, +0.95). Changes also included farmers' habits while applying pesticides, such as less smoking (PD -0.20; CI-0.34, -0.07) and eating at the work place (PD -0.33; CI-0.47, -0.19). No significant changes were found from 1998 to 2006 regarding use of personal protective equipment, pesticide storage, farmers' habits after applying pesticides, and in using some highly hazardous pesticides.</p> <p>Conclusions</p> <p>The results were based on two cross-sectional surveys and should be interpreted with caution due to potential validity problems. The results of the study suggest some positive changes in the handling of pesticides amongst participants in 2006, which could be due to different policy interventions and regulations that were implemented after 1998. However, farm workers in Beit -U'mmar village are still at risk of health effects because of ongoing exposure to pesticides. To the best of our knowledge, no studies on long-term changes in pesticide use have been reported from developing countries.</p

Association between the NBS1 E185Q polymorphism and cancer risk: a meta-analysis

<p>Abstract</p> <p>Background</p> <p>NBS1 is a key DNA repair protein in the homologous recombination repair pathway and a signal modifier in the intra-S phase checkpoint that plays important roles in maintaining genomic stability. The <it>NBS1 </it>8360G>C (<it>Glu185Gln</it>) is one of the most commonly studied polymorphisms of the gene for their association with risk of cancers, but the results are conflicting.</p> <p>Methods</p> <p>We performed a meta-analysis using 16 eligible case-control studies (including 17 data sets) with a total of 9,734 patients and 10,325 controls to summarize the data on the association between the <it>NBS1 </it>8360G>C (E185Q) polymorphism and cancer risk.</p> <p>Results</p> <p>Compared with the common 8360GG genotype, the carriers of variant genotypes (i.e., 8360 GC/CC) had a 1.06-fold elevated risk of cancer (95% CI = 1.00–1.12, <it>P </it>= 0.05) in a dominant genetic model as estimated in a fixed effect model. However, the association was not found in an additive genetic model (CC <it>vs </it>GG) (odds ratio, OR = 0.98, 95% CI = 0.85–1.13, <it>P </it>= 0.78) nor in a recessive genetic model (CC <it>vs </it>GC +GG) (OR = 0.94, 95% CI = 0.82–1.07, <it>P </it>= 0.36). The effect of the 8360G>C (E185Q) polymorphism was further evaluated in stratification analysis. It was demonstrated that the increased risk of cancer associated with 8360G>C variant genotypes was more pronounced in the Caucasians (OR = 1.07, 95% CI = 1.01–1.14, <it>P </it>= 0.03).</p> <p>Conclusion</p> <p>Our meta-analysis suggests that the <it>NBS1 </it>E185Q variant genotypes (8360 <it>GC/CC</it>) might be associated with an increased risk of cancer, especially in Caucasians.</p