Linajes uniparentales en una población mocoví de Chaco

Se analizaron muestras de hisopado bucal de individuos autodenominados Mocoví provenientes de la pcia. de Chaco (N=13). En las mismas se estudiaron los linajes uniparentales maternos y paternos. Para los primeros se analizó el ADNmit en busca de los haplogrupos A, B, C, D, y E. Para los linajes paternos se analizó la presencia de la transición C a T del marcador DYS199. En los linajes maternos se encontraron los haplogrupos A1, A2, D2 y C2. Los más representados fueron A1 y C2 con igual frecuencia (0,33). En los linajes paternos la frecuencia de los alelos C y T en el locus DYS199 fue de 0,58 y 0,42 respectivamente. Al comparar la muestra con datos preexistentes de una colonia mocoví de la provincia de Santa Fe (N=69) se encontraron diferencias significativas en la variabilidad de los linajes maternos. No puede descartarse que estas diferencias se deban al tamaño muestreal de la población chaqueña. No se encontraron diferencias significativas en cuanto a los linajes paternos. Éstos evidenciaron mezcla génica con europeos en ambas poblaciones, mientras que solo se encontraron linajes maternos de origen americanos.Asociación de Antropología Biológica de la República Argentin

Linajes uniparentales en una población mocoví de Chaco

Se analizaron muestras de hisopado bucal de individuos autodenominados Mocoví provenientes de la pcia. de Chaco (N=13). En las mismas se estudiaron los linajes uniparentales maternos y paternos. Para los primeros se analizó el ADNmit en busca de los haplogrupos A, B, C, D, y E. Para los linajes paternos se analizó la presencia de la transición C a T del marcador DYS199. En los linajes maternos se encontraron los haplogrupos A1, A2, D2 y C2. Los más representados fueron A1 y C2 con igual frecuencia (0,33). En los linajes paternos la frecuencia de los alelos C y T en el locus DYS199 fue de 0,58 y 0,42 respectivamente. Al comparar la muestra con datos preexistentes de una colonia mocoví de la provincia de Santa Fe (N=69) se encontraron diferencias significativas en la variabilidad de los linajes maternos. No puede descartarse que estas diferencias se deban al tamaño muestreal de la población chaqueña. No se encontraron diferencias significativas en cuanto a los linajes paternos. Éstos evidenciaron mezcla génica con europeos en ambas poblaciones, mientras que solo se encontraron linajes maternos de origen americanos.Asociación de Antropología Biológica de la República Argentin

Transverse Load and Temperature Sensing Using Multiplexed Long-Period Fiber Gratings

The simultaneous measurement of transverse load and temperature using two long-period fiber gratings multiplexed in the wavelength domain is presented experimentally. For this, a mechanically induced long-period fiber grating (MI-LPFG) and a long-period fiber grating inscribed by a continuous-wave CO2 laser (CO2 LPFG) are connected in cascade. First, the transverse load and the temperature measurements were individually performed by the multiplexed long-period fiber gratings configuration. The MI-LPFG is subject to a transverse load variation from 0–2000 g with steps of 500 g, whereas the CO2 LPFG is unloaded and they are kept at room temperature. Similarly, the CO2 LPFG is subject to a temperature variation from 30 to 110 °C by increments of 20 °C, while the MI-LPFG with a constant transverse load of 2000 g is kept at room temperature. Subsequently, the simultaneous measurement of the transverse load and the temperature is performed by the multiplexed long-period fiber grating following the steps outlined above. According to the experimental results, the transverse load and temperature measurement present high repeatability for the individual and simultaneous process. Moreover, the multiplexed LPFGs exhibit low cladding-mode crosstalk of transverse load and temperature. The coarse wavelength-division multiplexing (CWDM) of long-period fiber gratings is an attractive alternative technique in optical fiber distributed sensing applications

Continental Origin for Q Haplogroup Patrilineages in Argentina and Paraguay

Haplogroup Q originated in Eurasia around 30,000 years ago. It is present in Y-chromosomes from Asia and Europe at rather low frequencies. Since America is undoubtedly one of the continents where this haplogroup is highly represented, it has been defined as one of the founding haplogroups. Its M3 clade has been early described as the most frequent, with Pan-American representation. However, it was also possible to find several other haplogroup Q clades at low frequencies. Numerous mutations have been described for haplogroup Q, allowing the analysis of its variability and the assignment of its geographic origin. We have analyzed 442 samples belonging to haplogroup Q of unrelated men from Argentina and Paraguay, but this work is specifically referred to 27 Q (xM3) lineages. We tested 3 SNPs by APLP, 3 for RFLP, 15 SNPs by Sanger sequencing, and 17 STRs. Our approach allowed us to identify 5 sub-haplogroups. Q-M3 and Q-CTS2730/Z780 are undoubtedly autochthonous lineages and represent the most frequent sub-haplogroups. With significant representation in self-defined aboriginal populations, their autochthonous status has been previously described. The aim of present work is to identify the continental origin of the remaining Q lineages. Thus, we analyzed the STR haplotypes for the samples of our series and compared them with haplotypes described by other authors for the rest of the world. Even when haplogroup Qs have been extensively studied in America, some of them could have their origin in post Columbian human migration from Europe and Middle East

Regulation of a rat VL30 element in human breast cancer cells in hypoxia and anoxia: role of HIF-1

Novel approaches to cancer gene therapy currently exploit tumour hypoxia to achieve transcriptional targeting using oxygen-regulated enhancer elements called hypoxia response elements. The activity of such elements in hypoxic cells is directly dependent on upregulation of the hypoxia-inducible transcription factor-1 However tumours also contain areas of anoxia, which may be considered a more tumour-selective transcriptional stimulus than hypoxia for targeting gene therapy to tumours. Another element, from the rat virus-like retrotransposon, VL30 (termed the ‘secondary anoxia response element’) has been reported to be more highly inducible in rat fibroblasts under anoxia than hypoxia. To investigate anoxia as a potential transcriptional target in human tumours, we have examined secondary anoxia response element inducibility in two human breast cancer cell lines, MCF-7 and T47D, under anoxia, hypoxia and normoxia. In both cell types, the trimerised secondary anoxia response element showed greater inducibility in anoxia than hypoxia (1% and 0.5% O2). The anoxic response of the secondary anoxia response element was shown to be dependent on hypoxia-inducible transcription factor-1 and the presence of a hypoxia-inducible transcription binding site consensus (5′-ACGTG-3′). Mutational analysis demonstrated that the base immediately 5′ to this modulates the anoxic/hypoxic induction of the secondary anoxia response element, such that TACGTG>GACGTG>>CACGTG. A similar correlation was found for erythropoietin, phosphoglycerate kinase 1, and aldolase hypoxia response elements, which contain these respective 5′ flanking bases

PhcrTx2, a new crab-paralyzing peptide toxin from the sea anemone <i>Phymanthus crucifer</i>

Sea anemones produce proteinaceous toxins for predation and defense, including peptide toxins that act on a large variety of ion channels of pharmacological and biomedical interest. Phymanthus crucifer is commonly found in the Caribbean Sea; however, the chemical structure and biological activity of its toxins remain unknown, with the exception of PhcrTx1, an acid-sensing ion channel (ASIC) inhibitor. Therefore, in the present work, we focused on the isolation and characterization of new P. crucifer toxins by chromatographic fractionation, followed by a toxicity screening on crabs, an evaluation of ion channels, and sequence analysis. Five groups of toxic chromatographic fractions were found, and a new paralyzing toxin was purified and named PhcrTx2. The toxin inhibited glutamate-gated currents in snail neurons (maximum inhibition of 35%, IC50 4.7 µM), and displayed little or no influence on voltage-sensitive sodium/potassium channels in snail and rat dorsal root ganglion (DRG) neurons, nor on a variety of cloned voltage-gated ion channels. The toxin sequence was fully elucidated by Edman degradation. PhcrTx2 is a new β-defensin-fold peptide that shares a sequence similarity to type 3 potassium channels toxins. However, its low activity on the evaluated ion channels suggests that its molecular target remains unknown. PhcrTx2 is the first known paralyzing toxin in the family Phymanthidae

A method for genome-wide analysis of DNA helical tension by means of psoralen–DNA photobinding

The helical tension of chromosomal DNA is one of the epigenetic landmarks most difficult to examine experimentally. The occurrence of DNA crosslinks mediated by psoralen photobinding (PB) stands as the only suitable probe for assessing this problem. PB is affected by chromatin structure when is done to saturation; but it is mainly determined by DNA helical tension when it is done to very low hit conditions. Hence, we developed a method for genome-wide analysis of DNA helical tension based on PB. We adjusted in vitro PB conditions that discern DNA helical tension and applied them to Saccharomyces cerevisiae cells. We selected the in vivo cross-linked DNA sequences and identified them on DNA arrays. The entire procedure was robust. Comparison of PB obtained in vivo with that obtained in vitro with naked DNA revealed that numerous chromosomal regions had deviated PB values. Similar analyses in yeast topoisomerase mutants uncovered further PB alterations across specific chromosomal domains. These results suggest that distinct chromosome compartments might confine different levels of DNA helical tension in yeast. Genome-wide analysis of psoralen–DNA PB can be, therefore, a useful approach to uncover a trait of the chromosome architecture not amenable to other techniques

B7-H4 gene polymorphisms are associated with sporadic breast cancer in a Chinese Han population

© 2009 Zhang et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licens

Using a normative framework to explore the prototyping of wireless grids

The capacity for normative frameworks to capture the essential features of interactions between components in open architectures suggests they might also be of assistance in an early, rapid prototyping phase of system development, helping to refine concepts, identify actors, explore policies and evaluate feasibility. As an exercise to examine this thesis, we investigate the concept of the wireless grid. Wireless grids have been proposed to address the energy issues arising from a new generation of mobile phones, the idea being that local communication with other mobile phones, being cheaper, can be used in combination with network communication to achieve common goals while at the same time extending the battery duty cycle. This results in a social dilemma, as it is advantageous for rational users to benefit from the energy savings without any contribution to the cooperation, as every commitment has its price. We present a necessarily simplified model, whose purpose is to provide us with the foundation to explore issues in the management of such a framework, policies to encourage collaborative behaviour, and the means to evaluate the effects on energy consumption

Nucleosome Chiral Transition under Positive Torsional Stress in Single Chromatin Fibers

Using magnetic tweezers to investigate the mechanical response of single chromatin fibers, we show that fibers submitted to large positive torsion transiently trap positive turns, at a rate of one turn per nucleosome. A comparison with the response of fibers of tetrasomes (the (H3-H4)2 tetramer bound with ~50 bp of DNA) obtained by depletion of H2A-H2B dimers, suggests that the trapping reflects a nucleosome chiral transition to a metastable form built on the previously documented righthanded tetrasome. In view of its low energy, <8 kT, we propose this transition is physiologically relevant and serves to break the docking of the dimers on the tetramer which in the absence of other factors exerts a strong block against elongation of transcription by the main RNA polymerase.Comment: 33 pages (double spacing), 7 figure