Prediction of Displacements in Unstable Areas Using a Neural Model

In pipeline management the accurate prediction of weak displacements is a crucial factor in drawing up a prevention policy since the accumulation of these displacements over a period of several years can lead to situations of high risk. This work addresses the specific problem related to the prediction of displacements induced by rainfall in unstable areas, of known geology, and crossed by underground pipelines. A neural model has been configured which learns of displacements from instrumented sites (where inclinometric measurements are available) and is able to generalise to other sites not equipped with inclinometers

Biochemical Diversification through Foreign Gene Expression in Bdelloid Rotifers

Bdelloid rotifers are microinvertebrates with unique characteristics: they have survived tens of millions of years without sexual reproduction; they withstand extreme desiccation by undergoing anhydrobiosis; and they tolerate very high levels of ionizing radiation. Recent evidence suggests that subtelomeric regions of the bdelloid genome contain sequences originating from other organisms by horizontal gene transfer (HGT), of which some are known to be transcribed. However, the extent to which foreign gene expression plays a role in bdelloid physiology is unknown. We address this in the first large scale analysis of the transcriptome of the bdelloid Adineta ricciae: cDNA libraries from hydrated and desiccated bdelloids were subjected to massively parallel sequencing and assembled transcripts compared against the UniProtKB database by blastx to identify their putative products. Of ∼29,000 matched transcripts, ∼10% were inferred from blastx matches to be horizontally acquired, mainly from eubacteria but also from fungi, protists, and algae. After allowing for possible sources of error, the rate of HGT is at least 8%–9%, a level significantly higher than other invertebrates. We verified their foreign nature by phylogenetic analysis and by demonstrating linkage of foreign genes with metazoan genes in the bdelloid genome. Approximately 80% of horizontally acquired genes expressed in bdelloids code for enzymes, and these represent 39% of enzymes in identified pathways. Many enzymes encoded by foreign genes enhance biochemistry in bdelloids compared to other metazoans, for example, by potentiating toxin degradation or generation of antioxidants and key metabolites. They also supplement, and occasionally potentially replace, existing metazoan functions. Bdelloid rotifers therefore express horizontally acquired genes on a scale unprecedented in animals, and foreign genes make a profound contribution to their metabolism. This represents a potential mechanism for ancient asexuals to adapt rapidly to changing environments and thereby persist over long evolutionary time periods in the absence of sex

Relationship between haemostatic variables and the progression of carotid atherosclerosis

Increases in the thickness of common carotid intima-media (CC-IMT), as measured by B-mode ultrasonography, have been widely used in both population studies and clinical trials in the search for risk factors for early atherosclerosis progression. In this study we have investigated the relations between several baseline haemostatic and conventional risk factors and CC-IMT changes over 16 months in 64 peripheral arterial disease (PAD) patients, randomly selected from the prospective PLAT study series. Samples from 24 (37.5%) patients who showed increases in CC-IMT during the follow-up period were compared with those from 40 (62.5%) in which CC-IMT remained unchanged. Baseline conventional risk factors and coagulation variables were similar in the two groups except for higher plasma concentrations of von Willebrand factor (vWF) (178.3\ub153.6 vs 141.2\ub153.7 SD%, p=0.01) and Factor VII (FVII) (133 .9\ub136.4 vs 107.0\ub127.3, p=0.001) in the patients with increased CC-IMT. CC-IMT increases correlated positively with plasma levels of FVII (r=0 .31, p<0.01) and vWF (r=0.31, p<0.31 ). Multiple stepwise regression analysis identified FVII as the only independent variable associated with an increase in CC-IMT (\uf062=0.83 p<0.01). Thus, high plasma concentration of FVII and vWF may be associated with the progression of early carotid atherosclerosis in PAD patients

Conceptual Architecture and Service-oriented Implementation of a Regional Geoportal for Rice Monitoring

Agricultural monitoring has greatly benefited from the increased availability of a wide variety of remote-sensed satellite imagery, ground-sensed data (e.g., weather station networks) and crop models, delivering a wealth of actionable information to stakeholders to better streamline and improve agricultural practices. Nevertheless, as the degree of sophistication of agriculture monitoring systems increases, significant challenges arise due to the handling and integration of multi-scale data sources to present information to decision-makers in a way which is useful, understandable and user friendly. To address these issues, in this article we present the conceptual architecture and service-oriented implementation of a regional geoportal, specifically focused on rice crop monitoring in order to perform unified monitoring with a supporting system at regional scale. It is capable of storing, processing, managing, serving and visualizing monitoring and generated data products with different granularity and originating from different data sources. Specifically, we focus on data sources and data flow, and their importance for and in relation to different stakeholders. In the context of an EU-funded research project, we present an implementation of the regional geoportal for rice monitoring, which is currently in use in Europe’s three largest rice-producing countries, Italy, Greece and Spain

Hydrodynamics in evaporate-bearing fine-grained successions investigated through an interdisciplinary approach : A test study in southern Italy-hydrogeological behaviour of heterogeneous low-permeability media

Messinian evaporates are widely distributed in the Mediterranean Sea as outcropping sediments in small marginal basins and in marine cores. Progressive filling of subbasins led to the formation of complex aquifer systems in different regions where hypersaline and fresh water coexist and interact in different manner. It also generates a significant diversification of groundwater hydrochemical signature and different microbial communities. In the case study, the hydrogeology and hydrochemistry of the whole system are influenced by good hydraulic connection between the shallower pyroclastic horizon and the underlying evaporate-bearing fine-grained Messinian succession. This is demonstrated by the merge of hydrogeological, chemical, isotopic, and microbiological data. No mixing with deep ascending waters has been observed. As shown by geophysical, hydraulic, and microbiological investigations, the hydraulic heterogeneity of the Messinian bedrock, mainly due to karstified evaporitic interstrata/lenses, causes the hydraulic head to significantly vary with depth. Somewhere, the head increases with the depth's increase and artesian flow conditions are locally observed. Moreover, the metagenomic investigations demonstrated the existence of a poor hydraulic connection within the evaporate-bearing fine-grained succession at metric and decametric scales, therefore leading to a patchwork of geochemical (and microbiological) subenvironments

Variabilidad 3d de las aguas subterráneas en el Delta del Río Paraná. Argentina

En los sistemas costeros, el asumir simplificaciones de que los flujos se comportan bidimensionalmente, puede llevar a obtener conclusiones que no representen el real funcionamiento del sistema natural. En el caso el Delta del Río Paraná, esta variación temporo-espacial alcanza mayor magnitud. El sistema actúa con características de flujo 3D, con mayor variabilidad en estiajes y/o crecidas prolongadas del Río Paraná, al ser esta la condición de borde temporal que tiene el mayor impacto en el sistema. La superposición de estos efectos, producen limitaciones en la productividad del sistema, por lo que es incapaz de sustentar a una población estable. El objetivo ha sido el de caracterizar el impacto de las aguas subterráneas en el sistema ambiental-productivo del Delta del Río Paraná, su vinculación con las áreas de descarga y sus efectos en la variación en vertical de la salinidad.In coastal systems, the simplifications assume that two-dimensional flows behave can lead to draw co nclusions that do not represent the actual natural system. For the Parana River Delta, the spatial-temporal variation achieves greater magnitude. The system works with 3D flow characteristics, with greater variability in droughts and / or floods prolonged Paraná River, as this is the temporal boundary condition that has the greatest impact on the system. The superposition of these effects, constraints occur in the system productivity, so is unable to sustain a stable population. The aim has been to characterize the impact of groundwater in the environmental system-productive Parana River Delta, its connection with the discharge areas and their effects on the vertical variation in salinity.Universidad Nacional de La Plat

Identification of mobile lipids in human cancer tissues by ex vivo diffusion edited HR-MAS MRS

Magnetic Resonance Spectroscopy visible mobilelipids are considered important markers in the diagnosis ofhuman cancer and are thought to be closely involved in variousaspects of tumour transformation, such as cell proliferation,necrosis, apoptosis, hypoxia and drug resistance. A methodallowing the straightforward identification of the lipid classescontributing to the mobile lipids in human malignant tissuesis highly advisable. Ex vivo High Resolution Magic AngleSpinning Magnetic Resonance Spectroscopy was done directlyon human cerebral, renal and colorectal malignant tissuespecimens. A diffusion edited sequence, based on stimulatedecho and bipolar gradient pulses, was used to characterizemolecules with low diffusion rates, arising from mobile lipidcomponents. Cholesterol, triglycerides and phosphatidylcholineare simultaneously detected and all contribute to the mobilelipid resonances present in malignant glioma and clear cellrenal carcinoma tissue specimens spectra. On the contrary,papillary cell renal carcinoma spectrum is predominated byphosphatidylcholine resonances and that of colorectal adenocarcinoma is characterized by signals arising from triglycerides. Ex vivo diffusion edited High Resolution Magic AngleSpinning Magnetic Resonance Spectroscopy, done on intacttissue, is a powerful analytical tool to obtain a simple andimmediate identification of mobile lipid components. This can offer a significant contribution to better understanding their involvement in cancer tissues. Furthermore, ex vivo high resolution spectroscopic measurements allow to improve the interpretation of in vivo Magnetic Resonance spectra, increasing its clinical potentiality

Pre-operative micronutrient deficiencies in patients with severe obesity candidates for bariatric surgery

PURPOSE: In patients with obesity, micronutrient deficiencies have been reported both before and after bariatric surgery (BS). Obesity is a chronic pro-inflammatory status, and inflammation increases the risk of micronutrient malnutrition. Our objective was to assess in pre-BS patients the prevalence of micronutrient deficiencies and their correlation with blood values of C-reactive protein (CRP). METHODS: Anthropometric data, instrumental examinations, and blood variables were centrally measured in the first 200 patients undergoing a pre-BS evaluation at the “Città della Salute e della Scienza” Hospital of Torino, starting from January 2018. RESULTS: At least one micronutrient deficiency was present in 85.5% of pre-BS patients. Vitamin D deficiency was the most prevalent (74.5%), followed by folate (33.5%), iron (32%), calcium (13%), vitamin B12 (10%), and albumin (5.5%) deficiency. CRP values were high (> 5 mg/L) in 65% of the patients. These individuals showed increased rate of iron, folate, vitamin B12 deficiency, and a higher number of micronutrient deficiencies. In a multiple logistic regression model, increased CRP levels were significantly associated with deficiencies of vitamin B12 (OR = 5.84; 95% CI 1.25–27.2; p = 0.024), folate (OR = 4.02; 1.87–8.66; p < 0.001), and with the presence of ≥ 2 micronutrient deficiencies (OR = 2.31; 1.21–4.42; p = 0.01). CONCLUSIONS: Micronutrient deficiencies are common in patients with severe obesity undergoing BS, especially when inflammation is present. In the presence of increased CRP values before surgery, it might be advisable to search for possible multiple micronutrient deficiencies

ITA-MNGIE: an Italian regional and national survey for mitochondrial neuro-gastro-intestinal encephalomyopathy

Mitochondrial neuro-gastro-intestinal encephalomyopathy (MNGIE) is a rare and unavoidably fatal disease due to mutations in thymidine phosphorylase (TP). Clinically it is characterized by gastrointestinal dysfunction, malnutrition/cachexia and neurological manifestations. MNGIE diagnosis remains a challenge mainly because of the complexity and rarity of the disease. Thus, our purposes were to promote a better knowledge of the disease in Emilia-Romagna region (ERR) by creating an accurate and dedicated network; to establish the minimal prevalence of MNGIE in Italy starting from ERR. Blood TP activity level was used as screening test to direct candidates to complete diagnostic work-up. During the study period of 1 year, only 10/71 units of ERR recruited 14 candidates. Their screening did not show TP activity changes. An Italian patient not resident in ERR was actually proved to have MNGIE. At the end of study in Italy there were nine cases of MNGIE; thus, the Italian prevalence of the disease is ~0.15/1,000,000 as a gross estimation. Our study confirms that MNGIE diagnosis is a difficult process which reflects the rarity of the disease and, as a result, a low level of awareness among specialists and physicians. Having available novel therapeutic options (e.g., allogenic hematopoietic stem cell transplantation and, more recently, liver transplantation) and an easy screening test, an early diagnosis should be sought before tissue damage occurs irreversibly

UAV Remote Sensing for High-Throughput Phenotyping and for Yield Prediction of Miscanthus by Machine Learning Techniques

Miscanthus holds a great potential in the frame of the bioeconomy, and yield prediction can help improve Miscanthus’ logistic supply chain. Breeding programs in several countries are attempting to produce high-yielding Miscanthus hybrids better adapted to different climates and end-uses. Multispectral images acquired from unmanned aerial vehicles (UAVs) in Italy and in the UK in 2021 and 2022 were used to investigate the feasibility of high-throughput phenotyping (HTP) of novel Miscanthus hybrids for yield prediction and crop traits estimation. An intercalibration procedure was performed using simulated data from the PROSAIL model to link vegetation indices (VIs) derived from two different multispectral sensors. The random forest algorithm estimated with good accuracy yield traits (light interception, plant height, green leaf biomass, and standing biomass) using a VIs time series, and predicted yield using a peak descriptor derived from a VIs time series with 2.3 Mg DM ha−1 of the root mean square error (RMSE). The study demonstrates the potential of UAVs’ multispectral images in HTP applications and in yield prediction, providing important information needed to increase sustainable biomass production