The Drosophila Anion Exchanger (DAE) lacks a detectable interaction with the spectrin cytoskeleton

<p>Abstract</p> <p>Background</p> <p>Current models suggest that the spectrin cytoskeleton stabilizes interacting ion transport proteins at the plasma membrane. The human erythrocyte anion exchanger (AE1) was the first membrane transport protein found to be associated with the spectrin cytoskeleton. Here we evaluated a conserved anion exchanger from Drosophila (DAE) as a marker for studies of the downstream effects of spectrin cytoskeleton mutations.</p> <p>Results</p> <p>Sequence comparisons established that DAE belongs to the SLC4A1-3 subfamily of anion exchangers that includes human AE1. Striking sequence conservation was observed in the C-terminal membrane transport domain and parts of the N-terminal cytoplasmic domain, but not in the proposed ankyrin-binding site. Using an antibody raised against DAE and a recombinant transgene expressed in <it>Drosophila </it>S2 cells DAE was shown to be a 136 kd plasma membrane protein. A major site of expression was found in the stomach acid-secreting region of the larval midgut. DAE codistributed with an infolded subcompartment of the basal plasma membrane of interstitial cells. However, spectrin did not codistribute with DAE at this site or in anterior midgut cells that abundantly expressed both spectrin and DAE. Ubiquitous knockdown of DAE with dsRNA eliminated antibody staining and was lethal, indicating that DAE is an essential gene product in <it>Drosophila</it>.</p> <p>Conclusions</p> <p>Based on the lack of colocalization and the lack of sequence conservation at the ankyrin-binding site, it appears that the well-characterized interaction between AE1 and the spectrin cytoskeleton in erythrocytes is not conserved in <it>Drosophila</it>. The results establish a pattern in which most of the known interactions between the spectrin cytoskeleton and the plasma membrane in mammals do not appear to be conserved in <it>Drosophila</it>.</p

Remote Monitoring of Fish in Small Streams: A Unified Approach Using Pit Tags

Accurate assessments of fish populations are often limited by re-observation or recapture events. Since the early 1990s, passive integrated transponders (PIT tags) have been used to understand the biology of many fish species. Until recently, PIT applications in small streams have been limited to physical recapture events. To maximize recapture probability, we constructed PIT antenna arrays in small streams to remotely detect individual fish. Experiences from two different laboratories (three case studies) allowed us to develop a unified approach to applying PIT technology for enhancing data assessments. Information on equipment, its installation, tag considerations, and array construction is provided. Theoretical and practical definitions are introduced to standardize metrics for assessing detection efficiency. We demonstrate how certain conditions (stream discharge, vibration, and ambient radio frequency noise) affect the detection efficiency and suggest that by monitoring these conditions, expectations of efficiency can be modified. We emphasize the importance of consistently estimating detection efficiency for fisheries applications

Promoting Harmonious Work Passion among Unmotivated Employees: A Two-Nation Investigation of the Compensatory Function of Cooperative Psychological Climate

This research draws on self-determination theory to investigate (a) the role of cooperative psychological climate in promoting harmonious work passion among employees with low intrinsic motivation; and (b) the mediating role of harmonious passion in linking cooperative psychological climate to behavioral outcomes. We propose that cooperative psychological climate facilitates harmonious passion and, in particular, plays a compensatory role among employees with low intrinsic motivation. In turn, harmonious passion is expected to facilitate both task performance and interpersonal helping, thereby linking cooperative psychological climate to these employee behaviors. We test the model using data from employees and their supervisors across two countries (Singapore and Brazil) and find cross-national evidence that cooperative psychological climate compensates for low intrinsic motivation to predict harmonious passion. Harmonious passion also positively predicts task performance and interpersonal helping, but only in the Brazilian sample. This research enriches the nomological network of harmonious passion, provides an alternative pathway to driving employee passion when intrinsic motivation is lacking, and underscores the value of considering the joint roles of passion predictors so as to reap the performance benefits of harmonious work passion

Optical performance of the JWST MIRI flight model: characterization of the point spread function at high-resolution

The Mid Infra Red Instrument (MIRI) is one of the four instruments onboard the James Webb Space Telescope (JWST), providing imaging, coronagraphy and spectroscopy over the 5-28 microns band. To verify the optical performance of the instrument, extensive tests were performed at CEA on the flight model (FM) of the Mid-InfraRed IMager (MIRIM) at cryogenic temperatures and in the infrared. This paper reports on the point spread function (PSF) measurements at 5.6 microns, the shortest operating wavelength for imaging. At 5.6 microns the PSF is not Nyquist-sampled, so we use am original technique that combines a microscanning measurement strategy with a deconvolution algorithm to obtain an over-resolved MIRIM PSF. The microscanning consists in a sub-pixel scan of a point source on the focal plane. A data inversion method is used to reconstruct PSF images that are over-resolved by a factor of 7 compared to the native resolution of MIRI. We show that the FWHM of the high-resolution PSFs were 5-10% wider than that obtained with Zemax simulations. The main cause was identified as an out-of-specification tilt of the M4 mirror. After correction, two additional test campaigns were carried out, and we show that the shape of the PSF is conform to expectations. The FWHM of the PSFs are 0.18-0.20 arcsec, in agreement with simulations. 56.1-59.2% of the total encircled energy (normalized to a 5 arcsec radius) is contained within the first dark Airy ring, over the whole field of view. At longer wavelengths (7.7-25.5 microns), this percentage is 57-68%. MIRIM is thus compliant with the optical quality requirements. This characterization of the MIRIM PSF, as well as the deconvolution method presented here, are of particular importance, not only for the verification of the optical quality and the MIRI calibration, but also for scientific applications.Comment: 13 pages, submitted to SPIE Proceedings vol. 7731, Space Telescopes and Instrumentation 2010: Optical, Infrared, and Millimeter Wav

The warm interstellar medium around the Cygnus Loop

Observations of the oxygen lines [OII]3729 and [OIII]5007 in the medium immediately beyond the Cygnus Loop supernova remnant were carried out with the scanning Fabry-P\'erot spectrophotometer ESOP. Both lines were detected in three different directions - east, northeast and southwest - and up to a distance of 15 pc from the shock front. The ionized medium is in the immediate vicinity of the remnant, as evinced by the smooth brightening of both lines as the adiabatic shock transition (defined by the X-ray perimeter) is crossed. These lines are usually brighter around the Cygnus Loop than in the general background in directions where the galactic latitude is above 5 degrees. There is also marginal (but significant) evidence that the degree of ionization is somewhat larger around the Cygnus Loop. We conclude that the energy necessary to ionize this large bubble of gas could have been supplied by an O8 or O9 type progenitor or the particles heated by the expanding shock front. The second possibility, though highly atractive, would have to be assessed by extensive modelling.Comment: 18 pages, 8 figures, ApJ 512 in pres

Methodological enhancements in MDO process investigated in the AGILE European project

This paper presents methodological investigations performed in research activities in the field of MDO in overall aircraft design in the ongoing EU funded research project AGILE. AGILE is developing the next generation of aircraft Multidisciplinary Design and Optimization processes, which target significant reductions in aircraft development costs and time to market, leading to cheaper and greener aircraft solutions. The paper introduces the AGILE project structure and describes the achievements of the 1st year (Design Campaign 1) leading to a reference distributed MDO system. A focus is then made on the different novel optimization techniques studied during the 2nd year, all willing to ease the optimization of complex work flows, characterized by high degree of discipline interdependencies, high number of design variables in the context of multi-level and multi-partner collaborative engineering projects. Then the implementation of these methods in the enhanced MDO framework is discussed