Participatory modelling for stakeholder involvement in the development of flood risk management intervention options

Advancing stakeholder participation beyond consultation offers a range of benefits for local flood risk management, particularly as responsibilities are increasingly devolved to local levels. This paper details the design and implementation of a participatory approach to identify intervention options for managing local flood risk. Within this approach, Bayesian networks were used to generate a conceptual model of the local flood risk system, with a particular focus on how different interventions might achieve each of nine participant objectives. The model was co-constructed by flood risk experts and local stakeholders. The study employs a novel evaluative framework, examining both the process and its outcomes (short-term substantive and longer-term social benefits). It concludes that participatory modelling techniques can facilitate the identification of intervention options by a wide range of stakeholders, and prioritise a subset for further investigation. They can help support a broader move towards active stakeholder participation in local flood risk management

Genomic instability and telomere fusion of canine osteosarcoma cells.

Canine osteosarcoma (OSA) is known to present with highly variable and chaotic karyotypes, including hypodiploidy, hyperdiploidy, and increased numbers of metacentric chromosomes. The spectrum of genomic instabilities in canine OSA has significantly augmented the difficulty in clearly defining the biological and clinical significance of the observed cytogenetic abnormalities. In this study, eight canine OSA cell lines were used to investigate telomere fusions by fluorescence in situ hybridization (FISH) using a peptide nucleotide acid probe. We characterized each cell line by classical cytogenetic studies and cellular phenotypes including telomere associated factors and then evaluated correlations from this data. All eight canine OSA cell lines displayed increased abnormal metacentric chromosomes and exhibited numerous telomere fusions and interstitial telomeric signals. Also, as evidence of unstable telomeres, colocalization of γ-H2AX and telomere signals in interphase cells was observed. Each cell line was characterized by a combination of data representing cellular doubling time, DNA content, chromosome number, metacentric chromosome frequency, telomere signal level, cellular radiosensitivity, and DNA-PKcs protein expression level. We have also studied primary cultures from 10 spontaneous canine OSAs. Based on the observation of telomere aberrations in those primary cell cultures, we are reasonably certain that our observations in cell lines are not an artifact of prolonged culture. A correlation between telomere fusions and the other characteristics analyzed in our study could not be identified. However, it is important to note that all of the canine OSA samples exhibiting telomere fusion utilized in our study were telomerase positive. Pending further research regarding telomerase negative canine OSA cell lines, our findings may suggest telomere fusions can potentially serve as a novel marker for canine OSA

Mapping the climate sceptical blogosphere

While mainstream scientific knowledge production has been extensively examined in the academic literature, comparatively little is known about alternative networks of scientific knowledge production. Online sources such as blogs are an especially under-investigated site of knowledge contestation. Using degree centrality and node betweenness tests from social network analysis, and thematic content analysis of individual posts, this research identifies and critically examines the climate sceptical blogosphere and investigates whether a focus on particular themes contributes to the positioning of the most central blogs. A network of 171 individual blogs is identified, with three blogs in particular found to be the most central: Climate Audit, JoNova and Watts Up With That. These blogs predominantly focus on the scientific element of the climate debate, providing either a direct scientifically-based challenge to mainstream climate science, or a critique of the conduct of the climate science system. This overt scientific framing, as opposed to explicitly highlighting differences in values, politics, or ideological worldview, appears to be an important contributory factor in the positioning of the most central blogs. It is suggested that these central blogs are key protagonists in a process of attempted expert knowledge de-legitimisation and contestation, acting not only as translators between scientific research and lay audiences, but, in their reinterpretation of existing climate science knowledge claims, are acting themselves as alternative public sites of expertise for a climate sceptical audience