The High Angular Resolution Multiplicity of Massive Stars

We present the results of a speckle interferometric survey of Galactic massive stars that complements and expands upon a similar survey made over a decade ago. The speckle observations were made with the KPNO and CTIO 4 m telescopes and USNO speckle camera, and they are sensitive to the detection of binaries in the angular separation regime between 0.03" and 5" with relatively bright companions (Delta V < 3). We report on the discovery of companions to 14 OB stars. In total we resolved companions of 41 of 385 O-stars (11%), 4 of 37 Wolf-Rayet stars (11%), and 89 of 139 B-stars (64%; an enriched visual binary sample that we selected for future orbital determinations). We made a statistical analysis of the binary frequency among the subsample that are listed in the Galactic O Star Catalog by compiling published data on other visual companions detected through adaptive optics studies and/or noted in the Washington Double Star Catalog and by collecting published information on radial velocities and spectroscopic binaries. We find that the binary frequency is much higher among O-stars in clusters and associations compared to the numbers for field and runaway O-stars, consistent with predictions for the ejection processes for runaway stars. We present a first orbit for the O-star Delta Orionis, a linear solution of the close, apparently optical, companion of the O-star Iota Orionis, and an improved orbit of the Be star Delta Scorpii. Finally, we list astrometric data for another 249 resolved and 221 unresolved targets that are lower mass stars that we observed for various other science programs.Comment: 76 pages, 6 figures, 11 table

Visual orbit for the low-mass binary Gliese 22 AC from speckle interferometry

Based on 14 data points obtained with near-infrared speckle interferometry and covering an almost entire revolution, we present a first visual orbit for the low-mass binary system Gliese 22 AC. The quality of the orbit is largely improved with respect to previous astrometric solutions. The dynamical system mass is 0.592 +- 0.065 solar masses, where the largest part of the error is due to the Hipparcos parallax. A comparison of this dynamical mass with mass-luminosity relations on the lower main sequence and theoretical evolutionary models for low-mass objects shows that both probably underestimate the masses of M dwarfs. A mass estimate for the companion Gliese 22 C indicates that this object is a very low-mass star with a mass close to the hydrogen burning mass limit.Comment: Accepted by Astronomy and Astrophysics, 6 pages, 2 figure

Orbit Determination of Close Binary Systems using Lucky Imaging

We present relative positions of visual binaries observed during 2009 with the FastCam "lucky-imaging" camera at the 1.5-m Carlos Sanchez Telescope (TCS) at the Observatorio del Teide. We obtained 424 CCD observations (averaged in 198 mean relative positions) of 157 binaries with angular separations in the range 0.14-15.40", with a median separation of 0.51". For a given system, each CCD image represents the sum of the best 10-25% images from 1000-5000 short-exposure frames. Derived internal errors were 7 mas in r and 1.2^{\circ} (9 mas) in q. When comparing to systems with very well-known orbits, we find that the rms deviation in r residuals is 23 mas, while the rms deviation in q residuals is 0.73 deg/r. We confirmed 18 Hipparcos binaries and we report new companions to BVD 36 A and J 621 B. For binaries with preliminary orbital parameters, the relative radial velocity was estimated as well. We also present four new revised orbits computed for LDS 873, BU 627 A-BC, BU 628 and HO 197 AB. This work is the first results on visual binaries using the FastCam lucky-imaging camera.Comment: 23 pages, 10 figures, 14 tables, accepted August 18th, 2011, to be published in MNRA

Effect of RecA inactivation and detoxification systems on the evolution of ciprofloxacin resistance in Escherichia coli

Background Suppression of SOS response and overproduction of reactive oxygen species (ROS) through detoxification system suppression enhance the activity of fluoroquinolones. Objectives To evaluate the role of both systems in the evolution of resistance to ciprofloxacin in an isogenic model of Escherichia coli. Methods Single-gene deletion mutants of E. coli BW25113 (wild-type) (ΔrecA, ΔkatG, ΔkatE, ΔsodA, ΔsodB), double-gene (ΔrecA-ΔkatG, ΔrecA-ΔkatE, ΔrecA-ΔsodA, ΔrecA-ΔsodB, ΔkatG-ΔkatE, ΔsodB-ΔsodA) and triple-gene (ΔrecA-ΔkatG-ΔkatE) mutants were included. The response to sudden high ciprofloxacin pressure was evaluated by mutant prevention concentration (MPC). The gradual antimicrobial pressure response was evaluated through experimental evolution and antibiotic resistance assays. Results For E. coli BW25113 strain, ΔkatE, ΔsodB and ΔsodB/ΔsodA mutants, MPC values were 0.25 mg/L. The ΔkatG, ΔsodA, ΔkatG/katE and ΔrecA mutants showed 2-fold reductions (0.125 mg/L). The ΔkatG/ΔrecA, ΔkatE/ΔrecA, ΔsodA/ΔrecA, ΔsodB/ΔrecA and ΔkatG/ΔkatE/ΔrecA strains showed 4–8-fold reductions (0.03–0.06 mg/L) relative to the wild-type. Gradual antimicrobial pressure increased growth capacity for ΔsodA and ΔsodB and ΔsodB/ΔsodA mutants (no growth in 4 mg/L) compared with the wild-type (no growth in the range of 0.5–2 mg/L). Accordingly, increased growth was observed with the mutants ΔrecA/ΔkatG (no growth in 2 mg/L), ΔrecA/ΔkatE (no growth in 2 mg/L), ΔrecA/ΔsodA (no growth in 0.06 mg/L), ΔrecA/ΔsodB (no growth in 0.25 mg/L) and ΔrecA/ΔkatG/ΔkatE (no growth in 0.5 mg/L) compared with ΔrecA (no growth in the range of 0.002–0.015 mg/L). Conclusions After RecA inactivation, gradual exposure to ciprofloxacin reduces the evolution of resistance. After suppression of RecA and detoxification systems, sudden high exposure to ciprofloxacin reduces the evolution of resistance in E. coli.Plan Nacional de I+D+i 2013-2016 and the Instituto de Salud Carlos III (projects and PI17/01501 and PI20-00239)Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía, Industria y Competitividad, Spanish Network for Research in Infectious Diseases (REIPI; RD16/0016/0001 and REIPI RD16/ 0016/0009

Quinolone Resistance Reversion by Targeting the SOS Response

Suppression of the SOS response has been postulated as a therapeutic strategy for potentiating antimicrobial agents. We aimed to evaluate the impact of its suppression on reversing resistance using a model of isogenic strains of Escherichia coli representing multiple levels of quinolone resistance. E. coli mutants exhibiting a spectrum of SOS activity were constructed from isogenic strains carrying quinolone resistance mechanisms with susceptible and resistant phenotypes. Changes in susceptibility were evaluated by static (MICs) and dynamic (killing curves or flow cytometry) methodologies. A peritoneal sepsis murine model was used to evaluate in vivo impact. Suppression of the SOS response was capable of resensitizing mutant strains with genes encoding three or four different resistance mechanisms (up to 15-fold reductions in MICs). Killing curve assays showed a clear disadvantage for survival (Δlog10 CFU per milliliter [CFU/ml] of 8 log units after 24 h), and the in vivo efficacy of ciprofloxacin was significantly enhanced (Δlog10 CFU/g of 1.76 log units) in resistant strains with a suppressed SOS response. This effect was evident even after short periods (60 min) of exposure. Suppression of the SOS response reverses antimicrobial resistance across a range of E. coli phenotypes from reduced susceptibility to highly resistant, playing a significant role in increasing the in vivo efficacy

Impaired Virulence and In Vivo Fitness of Colistin-Resistant Acinetobacter baumannii

4 páginas, 2 figuras. Presentado en parte: 20 º Congreso Europeo de Microbiología Clínica y Enfermedades Infecciosas, Resumen 1389, Viena, Austria, 10-13 de abril de 2010.Acinetobacter baumannii (American Type Culture Collection strain 19606) acquires mutations in the pmrB gene during the in vitro development of resistance to colistin. The colistin-resistant strain has lower affinity for colistin, reduced in vivo fitness (competition index, .016), and decreased virulence, both in terms of mortality (0% lethal dose, 6.9 vs 4.9 log colony-forming units) and survival in a mouse model of peritoneal sepsis. These results may explain the low incidence and dissemination of colistin resistance in A. baumannii in clinical settings.This work was supported by the European Development Regional Fund “A way to achieve Europe” (ERDF); the Spanish Network for the Research in Infectious Diseases (REIPI RD06/0008), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación, Spain; and the Autonomous government of Madrid (COMBACT S-BIO-0260/2006, L.R.).Peer reviewe

Tracing the young massive high-eccentricity binary system Theta 1 Orionis C through periastron passage

The nearby high-mass star binary system Theta 1 Orionis C is the brightest and most massive of the Trapezium OB stars at the core of the Orion Nebula Cluster, and it represents a perfect laboratory to determine the fundamental parameters of young hot stars and to constrain the distance of the Orion Trapezium Cluster. Between January 2007 and March 2008, we observed T1OriC with VLTI/AMBER near-infrared (H- and K-band) long-baseline interferometry, as well as with bispectrum speckle interferometry with the ESO 3.6m and the BTA 6m telescopes (B'- and V'-band). Combining AMBER data taken with three different 3-telescope array configurations, we reconstructed the first VLTI/AMBER closure-phase aperture synthesis image, showing the T1OriC system with a resolution of approx. 2 mas. To extract the astrometric data from our spectrally dispersed AMBER data, we employed a new algorithm, which fits the wavelength-differential visibility and closure phase modulations along the H- and K-band and is insensitive to calibration errors induced, for instance, by changing atmospheric conditions. Our new astrometric measurements show that the companion has nearly completed one orbital revolution since its discovery in 1997. The derived orbital elements imply a short-period (P=11.3 yrs) and high-eccentricity orbit (e=0.6) with periastron passage around 2002.6. The new orbit is consistent with recently published radial velocity measurements, from which we can also derive the first direct constraints on the mass ratio of the binary components. We employ various methods to derive the system mass (M_system=44+/-7 M_sun) and the dynamical distance (d=410+/-20 pc), which is in remarkably good agreement with recently published trigonometric parallax measurements obtained with radio interferometry.Comment: 15 pages, 15 figures, accepted by A&