Urinary Carnosinase-1 Excretion is Associated with Urinary Carnosine Depletion and Risk of Graft Failure in Kidney Transplant Recipients: Results of the TransplantLines Cohort Study

Carnosine affords protection against oxidative and carbonyl stress, yet high concentrations of the carnosinase-1 enzyme may limit this. We recently reported that high urinary carnosinase-1 is associated with kidney function decline and albuminuria in patients with chronic kidney disease. We prospectively investigated whether urinary carnosinase-1 is associated with a high risk for development of late graft failure in kidney transplant recipients (KTRs). Carnosine and carnosinase-1 were measured in 24 h urine in a longitudinal cohort of 703 stable KTRs and 257 healthy controls. Cox regression was used to analyze the prospective data. Urinary carnosine excretions were significantly decreased in KTRs (26.5 [IQR 21.4-33.3] µmol/24 h versus 34.8 [IQR 25.6-46.8] µmol/24 h; p < 0.001). In KTRs, high urinary carnosinase-1 concentrations were associated with increased risk of undetectable urinary carnosine (OR 1.24, 95%CI [1.06-1.45]; p = 0.007). During median follow-up for 5.3 [4.5-6.0] years, 84 (12%) KTRs developed graft failure. In Cox regression analyses, high urinary carnosinase-1 excretions were associated with increased risk of graft failure (HR 1.73, 95%CI [1.44-2.08]; p < 0.001) independent of potential confounders. Since urinary carnosine is depleted and urinary carnosinase-1 imparts a higher risk for graft failure in KTRs, future studies determining the potential of carnosine supplementation in these patients are warranted

Cognitive effects of the progressive exercise test in electricians wearing personal protective equipment.

Objetivo: verificar o efeito do esfor?o f?sico sobre as fun??es cognitivas de trabalhadores eletricistas utilizando equipamento de prote??o individual (EPI). M?todos: participaram 28 eletricistas que trabalhavam na constru??o, manuten??o e opera??o de redes de distribui??o de energia. Todos do sexo masculino, sadios e aptos para a pr?tica de exerc?cios f?sicos. As fun??es cognitivas foram representadas pelo teste de rea??o simples (TRS) e pelo n?vel de vigil?ncia mental (NVM). O TRS e o NVM foram mensurados pr? e p?s- -teste m?ximo progressivo, em esteira rolante, a 27 ?C de temperatura seca e umidade relativa do ar de 64%. O teste consistiu em aumentos progressivos na velocidade e na inclina??o da esteira at? a fadiga, com utiliza??o de EPI. Resultados: a m?dia (desvio padr?o) do TRS n?o foi significativamente diferente antes, 227,8 (35,1) ms, e ap?s o exerc?cio, 220,6 (24,6) ms. O NVM foi significativamente maior ap?s o exerc?cio em todas as situa??es: frequ?ncia crescente ? 36,5 (5,1) Hz vs 39,5 (2,7) Hz, frequ?ncia decrescente ? 36,0 (5,2) vs 39,0 (3,88) Hz, e frequ?ncia geral ? 36,2 (4,9) vs 39,2 (3,1) Hz. Conclus?o: o exerc?cio progressivo m?ximo realizado com EPI n?o modificou o tempo de rea??o simples e aumentou o n?vel de vigil?ncia mental de eletricistas.Objective: to verify the effects of physical effort on the cognitive functions of electricians wearing personal protective equipment (PPE). Methods: 28 electricians participated. They worked on the construction, maintenance and operation of electrical power distribution networks. All were male, healthy and able to practice physical exercises. The cognitive functions were checked by measuring simple reaction time (SRT) and mental alertness level (MAL). SRT and MAL were measured before and after progressive maximal exercise, on a treadmill, at 27 ?C dry temperature and 64% relative humidity, wearing PPE. The test consisted of progressive increases in treadmill speed and incline, until fatigue. Results: SRT mean difference was not significantly different before ? 227.8 (35.1) ms ? and after exercising ?220.6 (24.6) ms. MAL was significantly higher after exercise in all situations: increasing frequency - 36.5 (5.1) Hz vs. 39.5 (2.7) Hz; decreasing frequency-36.0 (5.2) Hz vs. 39.0 (3.88) Hz; and general frequency 36.2 (4.9) Hz vs. 39.2 (3.1) Hz. Conclusion: progressive maximal exercise performed while wearing PPE caused no change in simple reaction time, and increased electricians? mental alertness level

Rabdomiólise em militares: uma missão de reconhecimento para prevenção.

O treinamento físico regular faz parte do cotidiano militar, com intuito de aprimorar competências físicas essenciais para tarefas de combate. No entanto, quando executado de forma extenuante ou desenvolvido em condições climáticas adversas pode ocasionar o desenvolvimento da rabdomiólise por esforço físico. A rabdomiólise por esforço físico é definida como uma síndrome decorrente da necrose das células musculares, com sintomas variáveis, como dor muscular, fraqueza e aumento plasmático de enzimas musculares. A severidade da rabdomiólise por esforço físico varia individualmente, porém, pode se tornar clinicamente relevante, contribuindo para a hospitalização, insuficiência renal aguda, incapacidade permanente ou óbito. Sendo assim, o objetivo desta revisão narrativa é apresentar possíveis causas e fatores de risco para rabdomiólise, discutir sua fisiopatologia, seus possíveis diagnósticos e suas principais complicações, enfatizando o contexto militar

El papel de los estudios bioarqueológicos en las interpretaciones sobre las comunidades neolíticas del noreste peninsular

Resumen del trabajo presentado al VI Congreso del Neolítico en la Península Ibérica: "Los cambios económicos y sus implicaciones sociales durante el Neolítico de la Península Ibérica", celebrado en Granada del 22 al 26 de junio de 2016.-- et al.El marco del proyecto I+D: “Aproximación a las primeras comunidades neolíticas del NE peninsular a través de sus prácticas funerarias” (2011-2015), y su continuidad para los próximos cuatro años al haber sido renovado, tiene por objetivo conocer cada día más y mejor las comunidades de agricultores y pastores que entre finales del V e inicios del IV milenio cal BC ocuparon y enterraron a sus muertos en el noreste de la Península Ibérica. El contexto de estudio es excepcional, puesto que aquellas comunidades inhumaron sistemáticamente a sus congéneres en tumbas habitualmente individuales y ocasionalmente junto a otro individuo. En esta presentación no sólo queremos mostrar los nuevos análisis y metodologías que estamos aplicando al estudio de los restos humanos, sino también el modelo de trabajo que hemos seguido. A este respecto, tres aspectos son fundamentales: 1) las dataciones absolutas son el eje que vertebra los posteriores análisis; 2) la colaboración con los distintos investigadores/as y laboratorios debe ser estrecha (no es cuestión de solicitar los resultados de un análisis a un laboratorio sino trabajar con las personas que manipulan las muestras y conocen los pros y contras de cada una de las técnicas empleadas) y 3) los estudios y análisis a realizar confluyen para responder a las hipótesis planteadas. A este respecto, en el proyecto hemos tenido la fortuna de poder colaborar con numerosos investigadores/as cuya especialidad versa alrededor de los restos funerarios y que firman la presente comunicación: análisis isotópicos, Adn, tafonomía funeraria, estudios de stress muscular y análisis de morfología dental.Peer Reviewe

Analysis of physical performance in children of seven to ten years old.

Capacidade f?sica ? o termo utilizado para agrupar as capacidades for?a, velocidade, resist?ncia, flexibilidade e coordena??o. O desempenho f?sico de crian?as ? preocupa??o permanente entre os especialistas da ?rea da sa?de. Esse interesse justifica-se j? que a atividade f?sica desempenha importante papel na preven??o de doen?as e melhoria da capacidade funcional. O objetivo do presente estudo foi analisar as capacidades f?sicas em crian?as de ambos os sexos. Ap?s consentimento por escrito dos pais, participaram do estudo 232 crian?as de ambos os sexos entre as idades de sete e 10 anos. A capacidade f?sica foi avaliada utilizando-se os testes de sentar e alcan?ar, resist?ncia abdominal, salto horizontal e corrida de 30 metros. Foi utilizado ANOVA two way e post hoc de Tukey para compara??o das vari?veis entre as idades e o sexo. O n?vel de signific?ncia foi de 5%. A flexibilidade n?o foi modificada pela idade e sexo. O salto horizontal e o n?mero de abdominais foram maiores nos meninos comparados ?s meninas e nas idades de 10, nove e oito anos comparados a idade de sete anos. O tempo para percorrer 30 m foi menor nos meninos comparado ?s meninas, sendo que a idade de 10 anos apresentou valores menores comparado as demais idades e as idades de nove e oito anos demonstraram menores valores comparado a idade de sete anos. De modo geral, os meninos apresentam melhor desempenho f?sico comparado ?s meninas na faixa et?ria avaliada. A idade de sete anos parece representar um per?odo de transi??o no desempenho f?sico.Physical performance is the term used to group strength, speed, endurance, flexibility and coordination capacities. The physical performance in the children is concern among experts in the field of health. This interest justified because physical activity can prevent illness and increase functional capacity. The aim of present study was assessment physical capacity in children of both sex, boys and girls. After consent of parents, concerns of study 232 children among seven and 10 years-old of both sexes. The performance motor was assessment using sit-and-reach, horizontal jump, abdominal endurance and 30 meters tests run. ANOVA Two-way and Tukey post hoc was used to compared variables among sex and ages. The significant level was 5%. Flexibility did not differ among ages and sex. The horizontal jump distance and abdominals number were higher in male group compared to the female group and eight, nine and 10 years-old were higher compared to seven years-old. The abdominals number was higher in male group compared to female group and eight, nine and 10 years-old are higher compared to seven years-old. The time to going 30 meters was small in the male group compared to female group and running time was small in the 10 years-old compared to other ages and the nine and eight years-old was small than seven years-old. In general, the boys presented better performance than girls in the ages assessment. The age seven years-old apparent represented a period of transition in the physical performance

Efeitos do cabelo da cabe?a humana no desempenho e na resposta termorregulat?ria durante a corrida de 10 km ao ar livre em homens saud?veis.

The aim of the present study was to evaluate the effects of human head hair on performance and thermoregulatory responses during 10-km outdoor running in healthy men. Twelve healthy males (29.5 ? 3.7 years, 174.9 ? 4.3 cm, 72.7 ? 3.2 kg and VO2max 44.6 ? 3.4 ml.kg-1.min-1) participated in two self-paced outdoor 10-km running trials separated by 7 days: 1) HAIR, subjects ran with their natural head hair; 2) NOHAIR, subjects ran after their hair had been totally shaved. Average running velocity was calculated from each 2-km running time. Rectal temperature, heart rate and physiological strain index were measured before and after the 10-km runs and at the end of each 2 km. The rate of heat storage was measured every 2 km. The environmental stress (WBGT) was measured every 10 min. The running velocity (10.9 ? 1 and 10.9 ? 1.1 km.h-1), heart rate (183 ? 10 and 180 ? 12 bpm), rectal temperature (38.82 ? 0.29 and 38.81 ? 0.49oC), physiological strain index (9 ? 1 and 9 ? 1), or heat storage rate (71.9 ? 64.1 and 80.7 ? 56.7 W.m-1) did not differ between the HAIR and NOHAIR conditions, respectively (p>0.05). There was no difference in WBGT between the HAIR and NOHAIR conditions (24.0 ? 1.4 and 23.2 ? 1.5?C, respectively; p=0.10). The results suggest that shaved head hair does not alter running velocity or thermoregulatory responses during 10-km running under the sun.O objetivo do presente estudo foi avaliar os efeitos do cabelo da cabe?a humana no desempenho e na resposta termorregulat?ria durante 10 km de corrida ao ar livre em homens saud?veis. Doze saud?vel do sexo masculino (29,5 ? 3,7 anos, 174,9 ? 4,3 cm, 72,7 ? 3,2 kg e VO2m?x 44,6 ? 3,4 ml.kg-1.min-1) participaram de 2 corridas de 10km separadas por 7 dias de intervalo em ritmo auto regulado: 1) HAIR- volunt?rios correram com seus cabelos intactos, 2) NOHAIR- volunt?rios correrram ap?s terem seus cabelos totalmente raspado. A velocidade m?dia da corrida foi calculada a cada s?rie de 2 km. Temperatura retal, freq??ncia card?aca e ?ndice de estresse fisiol?gico foram medidos antes e depois dos 10 km da corrida e no fim de cada 2 km. Taxa de armazenamento de calor foi medida a cada 2 km. Al?m disso, o estresse ambiental (WBGT) foi medido a cada 10 min. A velocidade de corrida (10,9 ? 1 e 10,9 ? 1,1 km.h-1), freq??ncia card?aca (183 ? 10 e 180 ? 12 bpm), temperatura retal (38,82 ? 0,29 e 38,81 ? 0,49oC), ?ndice estresse fisiol?gico (9 ? 1 e 9 ? 1) e taxa de armazenamento de calor (71,9 ? 64,1 e 80,7 ? 56,7 Wm-1), n?o foi diferente entre as situa??es HAIR e NOHAIR, respectivamente (p>0,05). N?o houve diferen?a no WBGT entre HAIR e NOHAIR (24,0 ? 1.4? C e 23,2 ? 1,5? C, respectivamente; p=0,10). Os resultados sugerem que raspar o cabelo da cabe?a n?o altera a velocidade da corrida e as respostas termorregulat?rias durante 10 km de corrida sob o sol

First narrow-band search for continuous gravitational waves from known pulsars in advanced detector data

Spinning neutron stars asymmetric with respect to their rotation axis are potential sources of continuous gravitational waves for ground-based interferometric detectors. In the case of known pulsars a fully coherent search, based on matched filtering, which uses the position and rotational parameters obtained from electromagnetic observations, can be carried out. Matched filtering maximizes the signalto- noise (SNR) ratio, but a large sensitivity loss is expected in case of even a very small mismatch between the assumed and the true signal parameters. For this reason, narrow-band analysis methods have been developed, allowing a fully coherent search for gravitational waves from known pulsars over a fraction of a hertz and several spin-down values. In this paper we describe a narrow-band search of 11 pulsars using data from Advanced LIGO’s first observing run. Although we have found several initial outliers, further studies show no significant evidence for the presence of a gravitational wave signal. Finally, we have placed upper limits on the signal strain amplitude lower than the spin-down limit for 5 of the 11 targets over the bands searched; in the case of J1813-1749 the spin-down limit has been beaten for the first time. For an additional 3 targets, the median upper limit across the search bands is below the spin-down limit. This is the most sensitive narrow-band search for continuous gravitational waves carried out so far

The Microbiome of Brazilian Mangrove Sediments as Revealed by Metagenomics

Here we embark in a deep metagenomic survey that revealed the taxonomic and potential metabolic pathways aspects of mangrove sediment microbiology. The extraction of DNA from sediment samples and the direct application of pyrosequencing resulted in approximately 215 Mb of data from four distinct mangrove areas (BrMgv01 to 04) in Brazil. The taxonomic approaches applied revealed the dominance of Deltaproteobacteria and Gammaproteobacteria in the samples. Paired statistical analysis showed higher proportions of specific taxonomic groups in each dataset. The metabolic reconstruction indicated the possible occurrence of processes modulated by the prevailing conditions found in mangrove sediments. In terms of carbon cycling, the sequences indicated the prevalence of genes involved in the metabolism of methane, formaldehyde, and carbon dioxide. With respect to the nitrogen cycle, evidence for sequences associated with dissimilatory reduction of nitrate, nitrogen immobilization, and denitrification was detected. Sequences related to the production of adenylsulfate, sulfite, and H2S were relevant to the sulphur cycle. These data indicate that the microbial core involved in methane, nitrogen, and sulphur metabolism consists mainly of Burkholderiaceae, Planctomycetaceae, Rhodobacteraceae, and Desulfobacteraceae. Comparison of our data to datasets from soil and sea samples resulted in the allotment of the mangrove sediments between those samples. The results of this study add valuable data about the composition of microbial communities in mangroves and also shed light on possible transformations promoted by microbial organisms in mangrove sediments