Molecular versus morphological markers to describe variability in sugar cane (Saccharum officinarum) for germplasm management and conservation = Marcadores moleculares y morfológicos para la descripción de variabilidad en caña de azúcar (Saccharum officinarum) con fines de manejo y conservación de germoplasma

Sugarcane is one of the most important industrial crops in tropical and subtropical regions. INTA (Argentina) administrates a Sugarcane Germplasm Bank and carries out a breeding program. The current study was designed to assess the phenotypic and genetic diversity among 65 sugarcane accessions selected from the INTA. Clustering and ordination methods based on quantitative and qualitative morphological traits and SSR data were applied. Generalized Procrustes Analysis allowed evaluating the correlation between relationships established with both markers A good of fit between dendrograms and similarity matrices were revealed by high cophenetic coefficients (r=0.82, p<0.0001; r=0.73, p<0.0001; r=0.82, p<0.0001 for phenotypic quantitative, phenotypic qualitative and molecular data respectively). The presence of different reliable population structure was observed when considering different data sources. Procrustes allowed finding those accessions that should have been responsible for the low correlation found between the individual configurations (73%). Both morphologic and molecular markers resulted discriminative enough to differentiate among accessions. It was not possible, however, to correlate associations of markers with the origin of materials. Phenotypic and genetic distances based on morphology and molecular information serves to assist conservation and organization of collection of materials, and the choice of parent combinations for breeding purposes.La caña de azúcar es uno de los cultivos industriales más importantes de regiones tropicales y subtropicales. El INTA (Argentina) administra un Banco de Germoplasma de caña de azúcar y lleva a cabo un programa de mejora. El presente trabajo fue diseñado para estimar la variabilidad fenotípica y genética entre 65 accesiones de caña de azúcar seleccionadas del INTA. Se aplicaron métodos de clasificación y ordenamiento en el análisis de datos morfológicos y de SSR. EL Análisis de Procrustes Generalizado permitió evaluar la correlación entre las relaciones establecidas a partir de ambos tipos de marca-dores. Un buen ajuste entre los dendrogramas y las matrices de similitud fue soportado por un alto coeficiente de correlación cofenética (r=0,82, p<0,0001; r=0,73, p<0,0001; r=0,82, p<0,0001 para datos cuantitativos, cualitativos y moleculares respectivamente). La presencia de una estructura poblacional fue reconocida cuando se consideraron los diferentes tipos de datos. El Procrustes permitió detectar aquellas accesiones que serían responsables de la baja correlación detectada entre configuraciones individuales (73%). Tanto los marcadores morfológicos como los moleculares resultaron lo suficientemente discriminativos para diferenciar accesiones. No obstante, no fue posible correlacionar las asociaciones establecidas por los marcadores con el origen de los materiales. Las distancias fenotípicas y genéticas basadas en información morfológica y molecular será de utilidad para asistir en la conservación y organización de los materiales de la colección y elegir combinaciones parentales con propósito de mejora.EEA FamailláFil: Pocovi, Mariana Ines. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; ArgentinaFil: Collavino, Norma Graciela. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; ArgentinaFil: Gutiérrez, Ángela. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; ArgentinaFil: Taboada, Gisel María. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; ArgentinaFil: Castillo, Verónica. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; ArgentinaFil: Delgado, Romina. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; ArgentinaFil: Mariotti Martinez, Jorge Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; Argentin

Design and implementation of a strategic process for an integrated management of the hydrologic resources of the Lerma-Chapala-Santiago watershed

La Red-Lerma tiene por objetivo facilitar y contribuir al fortalecimiento de las capacidades de la cuenca Lerma-Chapala-Santiago, a través de la generación conjunta de conocimientos, la formación de especialistas y la elaboración de alternativas a los problemas que los recursos hídricos enfrentan en la zona, permitiendo mejorar la calidad de vida de sus habitantes y su ambiente. En este contexto, la Red Lerma desarrolló una guía estructurada en torno a la Gestión Integrada de Recursos Hídricos (GIRH), con base en un proceso de planeación estratégica participativa, usando los indicadores Presión-Estado-Impacto-Respuesta y bajo un esquema de trabajo en Red, útil para los tomadores de decisiones de gobierno (Federal, Estatal y Municipal), organizaciones e instituciones, que convergen en in espacio inter-institucional e inter-disciplinario de una red local (Unidad Interdisciplinaria de Trabajo-UIT). En el ámbito regional, se han iniciado los procesos de integración de las UIT's en los estado de México, Nayarit, Guanajuato y Michoacán. Se participa además en el desarrollo y seguimiento del Plan Estratégico para la Recuperación de la Cuenca Alta del Río Lerma (CARL), así como en los trabajos de la Mesa Técnica de Educación Ambiental y Participación Social, ambos impulsados por la Universidad Autónoma del Estado de México y el Gobierno del Estado de México (GEM)

La antropología en la planificación regional como elemento para la gestión integrada de recursos hídricos

Aquí se considera que los escritos de Ángel Palerm sobre Planificación, son una excelente herramienta de introducción a la Gestión Integrada de los Recursos Hídricos (GIRH). De allí que el objetivo de este trabajo sea la revisión e identificación de las principales propuestas palermianas sobre los conceptos y herramientas de cómo llevar a cabo la planificación y el desarrollo, con el fi n de presentarlos como una introducción y reflexión a la gestión integrada de los recursos hídricos (GIRH). Para lograrlo, se analiza el material y los documentos reunidos y publicados de Ángel Palerm sobre el tema, que datan desde 1950 hasta 1980, ordenando los casos, los conceptos, las herramientas y las formas metodológicas que Palerm utilizó para la planificación

Metabolomics analysis of type 2 diabetes remission identifies 12 metabolites with predictive capacity: a CORDIOPREV clinical trial study.

Type 2 diabetes mellitus (T2DM) is one of the most widely spread diseases, affecting around 90% of the patients with diabetes. Metabolomics has proven useful in diabetes research discovering new biomarkers to assist in therapeutical studies and elucidating pathways of interest. However, this technique has not yet been applied to a cohort of patients that have remitted from T2DM. All patients with a newly diagnosed T2DM at baseline (n = 190) were included. An untargeted metabolomics approach was employed to identify metabolic differences between individuals who remitted (RE), and those who did not (non-RE) from T2DM, during a 5-year study of dietary intervention. The biostatistical pipeline consisted of an orthogonal projection on the latent structure discriminant analysis (O-PLS DA), a generalized linear model (GLM), a receiver operating characteristic (ROC), a DeLong test, a Cox regression, and pathway analyses. The model identified a significant increase in 12 metabolites in the non-RE group compared to the RE group. Cox proportional hazard models, calculated using these 12 metabolites, showed that patients in the high-score tercile had significantly (p-value < 0.001) higher remission probabilities (Hazard Ratio, HR, high versus low = 2.70) than those in the lowest tercile. The predictive power of these metabolites was further studied using GLMs and ROCs. The area under the curve (AUC) of the clinical variables alone is 0.61, but this increases up to 0.72 if the 12 metabolites are considered. A DeLong test shows that this difference is statistically significant (p-value = 0.01). Our study identified 12 endogenous metabolites with the potential to predict T2DM remission following a dietary intervention. These metabolites, combined with clinical variables, can be used to provide, in clinical practice, a more precise therapy. ClinicalTrials.gov, NCT00924937.The CORDIOPREV study is supported by the Ministerio de Economia y Competitividad, Spain, under the grants AGL2012/39615, PIE14/00005, and PIE14/00031 associated to J.L.-M.; AGL2015-67896-P to J.L.-M. and A.C.; CP14/00114 to A.C.; PI19/00299 to A.C.; DTS19/00007 to A.C.; FIS PI13/00023 to J.D.-L., PI16/01777 to F.P.-J. and P.P.-M.; Antonio Camargo is supported by an ISCIII research contract (Programa Miguel-Servet CPII19/00007); Marina Mora-Ortiz has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 847468; ‘Fundacion Patrimonio Comunal Olivarero’, Junta de Andalucía (Consejería de Salud, Consejeria de Agricultura y Pesca, Consejería de Innovacion, Ciencia y Empresa), ‘Diputaciones de Jaen y Cordoba’, ‘Centro de Excelencia en Investigación sobre Aceite de Oliva y Salud’ and ‘Ministerio de Medio Ambiente, Medio Rural y Marino’, Gobierno de España; ‘Consejeria de Innovación, Ciencia y Empresa, Proyectos de Investigación de Excelencia’, Junta de Andalucía under the grant CVI-7450 obtaiend by J.L.-M.; and we would also like to thank the ‘Fondo Europeo de Desarrollo Regional (FEDER)’.S

Quantitative Lipid Profiling Reveals Major Differences between Liver Organoids with Normal Pi*M and Deficient Pi*Z Variants of Alpha-1-antitrypsin.

Different mutations in the SERPINA1 gene result in alpha-1 antitrypsin (AAT) deficiency and in an increased risk for the development of liver diseases. More than 90% of severe deficiency patients are homozygous for Z (Glu342Lys) mutation. This mutation causes Z-AAT polymerization and intrahepatic accumulation which can result in hepatic alterations leading to steatosis, fibrosis, cirrhosis, and/or hepatocarcinoma. We aimed to investigate lipid status in hepatocytes carrying Z and normal M alleles of the SERPINA1 gene. Hepatic organoids were developed to investigate lipid alterations. Lipid accumulation in HepG2 cells overexpressing Z-AAT, as well as in patient-derived hepatic organoids from Pi*MZ and Pi*ZZ individuals, was evaluated by Oil-Red staining in comparison to HepG2 cells expressing M-AAT and liver organoids from Pi*MM controls. Furthermore, mass spectrometry-based lipidomics analysis and transcriptomic profiling were assessed in Pi*MZ and Pi*ZZ organoids. HepG2 cells expressing Z-AAT and liver organoids from Pi*MZ and Pi*ZZ patients showed intracellular accumulation of AAT and high numbers of lipid droplets. These latter paralleled with augmented intrahepatic lipids, and in particular altered proportion of triglycerides, cholesterol esters, and cardiolipins. According to transcriptomic analysis, Pi*ZZ organoids possess many alterations in genes and cellular processes of lipid metabolism with a specific impact on the endoplasmic reticulum, mitochondria, and peroxisome dysfunction. Our data reveal a relationship between intrahepatic accumulation of Z-AAT and alterations in lipid homeostasis, which implies that liver organoids provide an excellent model to study liver diseases related to the mutation of the SERPINA1 gene.This research was funded by INSTITUTO DE SALUD CARLOS III (ISCIII), grants numbers AESI PI20CIII/00015 and PT20CIII/00009, and the APC was funded by AESI PI20CIII/00015.S

Algorithm for the management of the geriatric patient at the anesthesia and geriatrics preoperative consultation

Introducción: los procedimientos quirúrgicos en pacientes adultos mayores son cada vez más frecuentes; se caracterizan por tener mayor morbimortalidad que en pacientes más jóvenes, por lo que es esencial una apropiada evaluación preoperatoria. Objetivo: diseñar un algoritmo para el adecuado tratamiento preoperatorio electivo del paciente geriátrico en el Hospital “Arnaldo Milián Castro”. Método: se realizó un estudio de desarrollo, descriptivo y transversal con enfoque cuantitativo y cualitativo. De un universo de 1 351 pacientes se seleccionó una muestra intencional de 1 346 pacientes que cumplieron criterios. Resultados: los adultos mayores acudieron con mayor frecuencia en los meses de febrero y agosto, predominó el sexo masculino y disminuyeron los casos en consultas a medida que aumentó la edad, Ranchuelo fue el municipio de mayor afluencia, prevalecieron los que tenían riesgo quirúrgico grado III y fueron diferidos, en su mayoría, los casos de mayor riesgo quirúrgico. Las Especialidades de Cirugía General y Ortopedia aportaron la mayor parte de los pacientes, aunque los que presentaron enfermedades ortopédicas fueron los más diferidos de la cirugía y las principales causas fueron la descompensación de enfermedades crónicas como la hipertensión arterial y la falta de complementarios. Conclusiones: con la participación de un grupo focal se elaboró una propuesta de algoritmo para el adecuado manejo de los adultos mayores que requieren interconsultas de Anestesia y Geriatría que fue valorada positivamente por expertos que emitieron sugerencias que permiten una propuesta de algoritmo más completa.Introduction: surgical procedures in elderly patients are increasingly frequent; they are characterized by a greater morbidity and mortality than in younger patients, so it is essential an appropriate preoperative assessment. Objective: to design an algorithm for the adequate elective preoperative treatment of the geriatric patient at the Arnaldo Milián Castro Hospital. Method: a descriptive and cross-sectional development study was conducted with a quantitative and qualitative approach. An intentional sample of 1346 patients, who met the inclusion criteria, was selected from a universe of 1351 patients. Results: older adults came with greater frequency in the months of February and August. Males predominated, and cases in consultations decreased as age increased. Ranchuelo was the municipality with greater inflow, and those patients who had surgical risk grade III predominated, and, in most cases, the cases of greater surgical risk were deferred. The Specialties of General Surgery and Orthopedics send most of the patients, although those who had orthopedic conditions were the most delayed in surgery, and the main causes were the decompensation of chronic diseases such as hypertension and the lack of complementary tests. Conclusions: with the participation of a focus group, an algorithm proposal was designed for the adequate management of older adults who need interconsultations of anesthesia and geriatrics. It was positively evaluated by experts who issued suggestions that allow a more complete algorithm proposal

NLRC4 -mediated activation of CD1c+ dendritic cells contributes to perpetuation of synovitis in rheumatoid arthritis.

The individual contribution of specific myeloid subsets such as CD1c+ conventional dendritic cells (cDC) to perpetuation of Rheumatoid Arthritis (RA) pathology remains unclear. In addition, the specific innate sensors driving pathogenic activation of CD1c+ cDCs in RA patients and their functional implications have not been characterized. Here, we assessed phenotypical, transcriptional and functional characteristics of CD1c+ and CD141+ cDCs and monocytes from the blood and synovial fluid of RA patients. Increased levels of CCR2 and the IgG receptor CD64 on circulating CD1c+ cDC associated with the presence of this DC subset in the synovial membrane in RA patients. Moreover, synovial CD1c+ cDCs are characterized by increased expression of proinflammatory cytokines and high abilities to induce pathogenic IFNγ+IL-17+ CD4+ T cells in vitro. Finally, we identified the crosstalk between Fcγ Receptors and NLRC4 as a new potential molecular mechanism mediating pathogenic activation, CD64 upregulation and functional specialization of CD1c+ cDCs in response to dsDNA-IgG in RA patients.E.M.G. was supported by Comunidad de Madrid Talento Program (2017-T1/BMD5396), Ramón y Cajal Program (RYC2018-024374-I), the MINECO RETOS program (RTI2018-097485-A-I00), La Caixa Foundation (HR20-00218), CIBERINF (CB21/13/00107) and the TV3 Marató (REDINCOV). C.D.A. was supported by Comunidad de Madrid Talento Program (2017-T1/BMD-5396). M.C.M was supported by the NIH (R21AI140930). HR17-00016 grant from “La Caixa Banking Foundation to F.S.M also supported the study. D.C-F. is supported by the Fellowship “la Caixa” Foundation LCF/BQ/DR19/11740010. A.T.M. was supported by a PhD fellowship from the Autonomous Region of Madrid (PEJD-2019-PRE/BMD-16851) and the RD21/0002/0027 grant. R.L. and G.H.B. were supported by PI18/00261 AND PI20/00349 grants from Ministerio de Ciencia e Innovación and Instituto de Salud Carlos III, respectively. O.P. was supported by the TV3 Marató (REDINCOV) and I.T. was funded by grant for the promotion of research studies master-UAM 2021. S.C. was supported by PI21/01474 grant from the Instituto de Salud Carlos III and co-funded by The European Regional Development Fund (ERDF). I.G.A was supported by RD21/0002/0027 and PI21/00526 grants from the Spanish MINECO and Instituto de Salud Carlos III and co-funded by The European Regional Development Fund (ERDF) “A way to make Europe.S

An intermittent extreme BL Lac: MWL study of 1ES 2344+514 in an enhanced state

Extreme high-frequency BL Lacs (EHBL) feature their synchrotron peak of the broad-band spectral energy distribution (SED) at vs ≥ 1017 Hz. The BL Lac object 1ES 2344+514 was included in the EHBL family because of its impressive shift of the synchrotron peak in 1996. During the following years, the source appeared to be in a low state without showing any extreme behaviours. In 2016 August, 1ES 2344+514 was detected with the groundbased γ -ray telescope FACT during a high γ -ray state, triggering multiwavelength (MWL) observations. We studied the MWL light curves of 1ES 2344+514 during the 2016 flaring state, using data from radio to very-high-energy (VHE) γ -rays taken with OVRO, KAIT, KVA, NOT, some telescopes of the GASP-WEBT collaboration at the Teide, Crimean, and St. Petersburg observatories, Swift-UVOT, Swift-XRT, Fermi-LAT, FACT, and MAGIC. With simultaneous observations of the flare, we built the broad-band SED and studied it in the framework of a leptonic and a hadronic model. The VHE γ -ray observations show a flux level of 55 per cent of the Crab Nebula flux above 300 GeV, similar to the historical maximum of 1995. The combination of MAGIC and Fermi-LAT spectra provides an unprecedented characterization of the inverse-Compton peak for this object during a flaring episode. The _ index of the intrinsic spectrum in the VHE γ -ray band is 2.04 ± 0.12stat ± 0.15sys.We find the source in an extreme state with a shift of the position of the synchrotron peak to frequencies above or equal to 1018 Hz.The financial support of the German BMBF and MPG, the Italian INFN and INAF, the Swiss National Fund SNF, the ERDF under the Spanish MINECO (FPA2017-87859-P, FPA2017-85668-P, FPA2017-82729-C6-2-R, FPA2017-82729-C6-6-R, FPA2017-82729-C6-5-R, AYA2015-71042-P, AYA2016-76012-C3-1-P, ESP2017-87055-C2-2-P, and FPA2017-90566-REDC), the Indian Department of Atomic Energy, the Japanese JSPS and MEXT, the Bulgarian Ministry of Education and Science, National RI Roadmap Project DO1-153/28.08.2018, and the Academy of Finland grant no. 320045 is gratefully acknowledged. This work was also supported by the Spanish Centro de Excelencia ‘Severo Ochoa’ SEV-2016-0588 and SEV-2015-0548, and Unidad de Excelencia ‘María de Maeztu’ MDM-2014-0369, by the Croatian Science Foundation (HrZZ) Project IP-2016-06-9782 and the University of Rijeka Project 13.12.1.3.02, by the DFG Collaborative Research Centers SFB823/C4 and SFB876/C3, the Polish National Research Centre grant UMO-2016/22/M/ST9/00382 and by the Brazilian MCTIC, CNPq, and FAPERJ. The FACT collaboration acknowledges the important contributions from ETH Zurich grants ETH-10.08-2 and ETH-27.12-1 as well as the funding by the Swiss SNF and the German BMBF (Verbundforschung Astro- und Astroteilchenphysik) and HAP (Helmoltz Alliance for Astroparticle Physics) are gratefully acknowledged. Part of this work is supported by Deutsche Forschungsgemeinschaft (DFG) within the Collaborative Research Center SFB 876 ‘Providing Information by Resource-Constrained Analysis’, project C3

Smart Solar Micro-exchangers for Sustainable Mobility of University Camps

Publicado el resumen en: https://www.wmcaus.org/files/WMCAUS2020_Book.pdf. Pendiente de publicación de las contribuciones en IOP Conference Series: Materials Science and Engineering.A significant number of universities have several campuses located in urban or rural settings, or with scattered university buildings that require the use of means of transportation. This implies the mobility and potential displacement of a large community of students, professors and researchers. The use of electric bicycles (e-bikes) is an intermediate alternative between the bicycle and electric cars. It can be an important stimulus for the promotion of the decarbonisation of the University Campus, avoiding the traffic congestion and reducing space requirements for parking. This paper presents the smart solar micro-exchanger model managed through a sustainable mobility web platform, applied to the case study of the University of Malaga (Spain). It is a solar charging station for e-bike, whose design is based on the principles of solar architecture (providing great security to e-bike). It managed by a web platform and app that allows the user to make reservations and learn about the savings in CO2 emissions. The system allows performing an aerobic sports activity without sweating problems when you reach the job. The platform also incorporates a database of quiet and safe routes for e-bike users.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Adverse drug reactions to the three doses of the severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) mRNA-1273 vaccine in a cohort of cancer patients under active treatment of a tertiary hospital in Madrid, Spain [version 2; peer review: 2 approved]

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines efficacy and safety have been tested in phase 3 studies in which cancer patients were not included or were underrepresented. Methods: The objective of this study is to evaluate the safety profile of the mRNA-1273 vaccine across cancer patients and its relationship to patients’ demographics. We selected from our records all 18-years or older solid cancer patients under active treatment vaccinated with the complete three-dose schedule mRNA-1273 vaccine whose adverse drug reactions (ADRs)  after each dose were recorded. Medical records were reviewed retrospectively to collect data between April 19, 2021, and December 31, 2021. Patients with documented previous infection by SARS-Cov-2 were excluded. Results: A total of 93 patients met the inclusion criteria. Local ADRs were reported more frequently after the first and second dose than after the third (41.9%, 43% and 31.1% of the patients respectively), while systemic ADRs followed the opposite pattern (16.1%, 34.4% and 52.6% of the patients respectively). We found a statistically significant association between sex and systemic adverse reactions after the third dose, p < 0.001 and between systemic adverse reactions after the second dose and systemic adverse reactions after the third dose, p = 0.001 A significant linear trend, p = 0.012, with a higher Eastern Cooperative Oncology Group (ECOG) score associated with a lower proportion of patients suffering from systemic side effects was found. Women had 5.79 times higher odds to exhibit systemic ADRs after the third dose (p=0.01) compared to males. Increasing age was associated with a decreased likelihood of exhibiting ADRs (p=0.016). Conclusion: The mRNA-1273 vaccine shows a tolerable safety profile. The likelihood of ADRs appears to be associated with gender and age. Its association with ECOG scores is less evident. Further studies are needed to elucidate this data in cancer patients