Cloning and characterisation of cDNAs encoding the major, pea storage proteins, and expression of vicilin in E.coli

A cDNA library was constructed using mRNA from developing seeds of pea (Pisim s'ativum L.). Clones encoding legumin and vicilin, the major storage proteins, were isolated and characterised, in several cases to the extent of complete DNA sequencing. The composite DNA sequence of the two longest legumin cDNAs extended over almost 90% of a complete legumin gene coding sequence. Both these clones contained three ~54bp tandem repeats in the region1encoding the acidic subunit. Evidence is presented that these repeats may be present in all chromosomal legumin genes and consequently, that the absence of the repeats from a previously isolated legumin cDNA probably represented a cloning artefact. Two, near full-length, vicilin cDNAs encoding 50000-Mr vicilin subunits, and another encoding a 47000-Mr subunit were sequenced. The 50000-Mr vicilin cDNAs were almost identical over most of their lengths, but one contained an artefactual, inverse repeat at its 3' terminus, while sequence differences at the 3' termini indicated the use of alternative polyadenylation sites. Comparisons of protein and cDNA-encoded amino acid sequences indicated that vicilins are synthesised as polypeptide precursors which subsequently undergo the removal of an N-terminal signal pep tide and possibly a C-terminal extension, as well as being susceptible to endo-proteolytic processing. Extending these comparisons to legumin and lectin sequences suggests that endo-proteolysis of these seed proteins occurs on the C-terminal side of asparagine residues located within B-turn conformations in hydrophilic regions of the proteins. Two vicilin cDNAs were expressed as both fused and unfused products in Escherichia coli under the influence of the phage lambda, leftward promoter (λP(_L)). Levels of expression obtained with different expression plasmid constructions supported previous hypotheses that translational efficiencies were lowered when the Shine-Dalgarno sequence was sequestered into double-stranded regions of the mRNA. There was also some indication that synthesis of a vicilin polypeptide bearing a signal peptide had a deleterious effect on the viability of the host strain

La Rivière-de-Corps – Rue Neuve

En amont d’un projet de lotissement sur la commune de La-Rivière-de-Corps, à l’est de la ville de Troyes, un diagnostic mené entre octobre et novembre 2006 par M. Kasprzyk (Inrap) a permis la mise au jour d’un site principalement attribuable à la période romaine sur la partie nord de l’aménagement. Libérés de toute contrainte archéologique, les deux tiers sud de la surface ont été aménagés et la partie nord abandonnée. En 2015, suite à la reprise par un autre aménageur de ce projet, une fouil..

Biofouling protection for marine environmental sensors

These days, many marine autonomous environment monitoring networks are set up in the world. These systems take advantage of existing superstructures such as offshore platforms, lightships, piers, breakwaters or are placed on specially designed buoys or underwater oceanographic structures. These systems commonly use various sensors to measure parameters such as dissolved oxygen, turbidity, conductivity, pH or fluorescence. Emphasis has to be put on the long term quality of measurements, yet sensors may face very short-term biofouling effects. Biofouling can disrupt the quality of the measurements, sometimes in less than a week. <br><br> Many techniques to prevent biofouling on instrumentation are listed and studied by researchers and manufacturers. Very few of them are implemented on instruments and of those very few have been tested in situ on oceanographic sensors for deployment of at least one or two months. <br><br> This paper presents a review of techniques used to protect against biofouling of in situ sensors and gives a short list and description of promising techniques

Acute Myeloid Leukemia: Focus on Novel Therapeutic Strategies

Acute myeloid leukemia (AML) is a heterogeneous disease with variable clinical outcomes. Cytogenetic analysis reveals which patients may have favorable risk disease, but 5-year survival in this category is only approximately 60%, with intermediate and poor risk groups faring far worse. Advances in our understanding of the biology of leukemia pathogenesis and prognosis have not been matched with clinical improvements. Unsatisfactory outcomes persist for the majority of patients with AML, particularly the elderly. Novel agents and treatment approaches are needed in the induction, post-remission and relapsed settings. The additions of clofarabine for relapsed or refractory disease and the hypomethylating agents represent recent advances. Clinical trials of FLT3 inhibitors have yielded disappointing results to date, with ongoing collaborations attempting to identify the optimal role for these agents. Potential leukemia stem cell targeted therapies and treatments in the setting of minimal residual disease are also under investigation. In this review, we will discuss recent advances in AML treatment and novel therapeutic strategies

Wireless Sensor Networks for Oceanographic Monitoring: A Systematic Review

Monitoring of the marine environment has come to be a field of scientific interest in the last ten years. The instruments used in this work have ranged from small-scale sensor networks to complex observation systems. Among small-scale networks, Wireless Sensor Networks (WSNs) are a highly attractive solution in that they are easy to deploy, operate and dismantle and are relatively inexpensive. The aim of this paper is to identify, appraise, select and synthesize all high quality research evidence relevant to the use of WSNs in oceanographic monitoring. The literature is systematically reviewed to offer an overview of the present state of this field of study and identify the principal resources that have been used to implement networks of this kind. Finally, this article details the challenges and difficulties that have to be overcome if these networks are to be successfully deployed

Sustained transatlantic coastal observations Report: Strategy for transatlantic sustained measurements in the coastal ocean, based on the strengthened forum for interaction between US IOOS, GOOS regional alliances and EuroGOOS

DoA: Task 4.4 Transatlantic cooperation and sustainability This task will enhance the European Forum for Coastal Technologies and establish a formal link between the US Alliance for Coastal Technologies and the European Forum for Coastal Technologies. We will strengthen links with coastal observing initiatives around the Atlantic basin including links between EuroGOOS ROOSes, and the regional operational oceanographic systems in the US and Canada. In doing so, we will create a forum for interaction between US IOOS, GOOS regional alliances, and EuroGOOS. The task will develop a strategy for transatlantic sustained measurements in the coastal ocean, building on developments such as OceanObs, Coastal GOOS and JERICO FP7 project, to deliver data for social and economic benefi

Toward a European coastal observing network to provide better answers to science and to societal challenges : The JERICO research infrastructure

The coastal area is the most productive and dynamic environment of the world ocean, offering significant resources and services for mankind. As exemplified by the UN Sustainable Development Goals, it has a tremendous potential for innovation and growth in blue economy sectors. Due to the inherent complexity of the natural system, the answers to many scientific and societal questions are unknown, and the impacts of the cumulative stresses imposed by anthropogenic pressures (such as pollution) and climate change are difficult to assess and forecast. A major challenge for the scientific community making observations of the coastal marine environment is to integrate observations of Essential Ocean Variables for physical, biogeochemical, and biological processes on appropriate spatial and temporal scales, and in a sustained and scientifically based manner. Coastal observations are important for improving our understanding of the complex biotic and abiotic processes in many fields of research such as ecosystem science, habitat protection, and climate change impacts. They are also important for improving our understanding of the impacts of human activities such as fishing and aquaculture, and underpin risk monitoring and assessment. The observations enable us to better understand ecosystems and the societal consequences of overfishing, disease (particularly shellfish), loss of biodiversity, coastline withdrawal, and ocean acidification, amongst others. The European coastal observing infrastructure JERICO-RI, has gathered and organized key communities embracing new technologies and providing a future strategy, with recommendations on the way forward and on governance. Particularly, the JERICO community acknowledges that the main providers of coastal observations are: (1) research infrastructures, (2) national monitoring programs, and (3) monitoring activities performed by marine industries. The scope of this paper is to present some key elements of our coastal science strategy to build it on long term. It describes how the pan-European JERICO community is building an integrated and innovation-driven coastal research infrastructure for Europe. The RI embraces emerging technologies which will revolutionize the way the ocean is observed. Developments in biotechnology (molecular and optical sensors, omics-based biology) will soon provide direct and online access to chemical and biological variables including in situ quantification of harmful algae and contaminants. Using artificial intelligence (AI), Internet of Things will soon provide operational platforms and autonomous and remotely operated smart sensors. Embracing key technologies, high quality open access data, modeling and satellite observations, it will support sustainable blue growth, warning and forecasting coastal services and healthy marine ecosystem. JERICO-FP7 is the European 7th framework project named JERICO under Grant Agreement No. 262584. JERICO-NEXT is the European Horizon-2020 project under Grant Agreement No. 654410. JERICO-RI is the European coastal observing research infrastructure established and structured through JERICO-FP7 and JERICO-NEXT, and beyond

Akaline, saline and mixed saline-alkaline stresses induce physiological and morpho-anatomical changes in Lotus tenuis shoots

Saline, alkaline and mixed saline–alkaline conditions frequently co-occur in soil. In this work, we compared these plant stress sources on the legume Lotus tenuis, regarding their effects on shoot growth and leaf and stem anatomy. In addition, we aimed to gain insight on the plant physiological status of stressed plants. We performed pot experiments with four treatments: control without salt (pH = 5.8; EC = 1.2 dS·m−1) and three stress conditions, saline (100 mm NaCl, pH = 5.8; EC = 11.0 dS·m−1), alkaline (10 mm NaHCO3, pH = 8.0, EC = 1.9 dS·m−1) and mixed salt–alkaline (10 mm NaHCO3 + 100 mm NaCl, pH = 8.0, EC = 11.0 dS·m−1). Neutral and alkaline salts produced a similar level of growth inhibition on L. tenuis shoots, whereas their mixture exacerbated their detrimental effects. Our results showed that none of the analysed morpho-anatomical parameters categorically differentiated one stress from the other. However, NaCl- and NaHCO3-derived stress could be discriminated to different extents and/or directions of changes in some of the anatomical traits. For example, alkalinity led to increased stomatal opening, unlike NaCl-treated plants, where a reduction in stomatal aperture was observed. Similarly, plants from the mixed saline–alkaline treatment characteristically lacked palisade mesophyll in their leaves. The stem cross-section and vessel areas, as well as the number of vascular bundles in the sectioned stem were reduced in all treatments. A rise in the number of vessel elements in the xylem was recorded in NaCl-treated plants, but not in those treated exclusively with NaHCO3.Fil: Paz, Rosalia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); ArgentinaFil: Reinoso, Herminda Elmira. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Biología Agrícola. Cátedra de Morfología Vegetal; ArgentinaFil: Espasandin, Fabiana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Gonzalez Antivilo, Francisco Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Rocco, Ruben Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); ArgentinaFil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); ArgentinaFil: Menendez, Ana Bernardina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentin