Staphylococcus aureus-Fibronectin Interactions with and without Fibronectin-Binding Proteins and Their Role in Adhesion and Desorption

Adhesion and residence-time-dependent desorption of two Staphylococcus aureus strains with and without fibronectin (Fn) binding proteins (FnBPs) on Fn-coated glass were compared under flow conditions. To obtain a better understanding of the role of Fn-FnBP binding, the adsorption enthalpies of Fn with staphylococcal cell surfaces were determined using isothermal titration calorimetry (ITC). Interaction forces between staphylococci and Fn coatings were measured using atomic force microscopy (AFM). The strain with FnBPs adhered faster and initially stronger to an Fn coating than the strain without FnBPs, and its Fn adsorption enthalpies were higher. The initial desorption was high for both strains but decreased substantially within 2 s. These time scales of staphylococcal bond ageing were confirmed by AFM adhesion force measurement. After exposure of either Fn coating or staphylococcal cell surfaces to bovine serum albumin (BSA), the adhesion of both strains to Fn coatings was reduced, suggesting that BSA suppresses not only nonspecific but also specific Fn-FnBP interactions. Adhesion forces and adsorption enthalpies were only slightly affected by BSA adsorption. This implies that under the mild contact conditions of convective diffusion in a flow chamber, adsorbed BSA prevents specific interactions but does allow forced Fn-FnBP binding during AFM or stirring in ITC. The bond strength energies calculated from retraction force-distance curves from AFM were orders of magnitude higher than those calculated from desorption data, confirming that a penetrating Fn-coated AFM tip probes multiple adhesins in the outermost cell surface that remain hidden during mild landing of an organism on an Fn-coated substratum, like that during convective diffusional flow

Sodium alginate and Chitosan aided design of form-stable Polyrotaxane based phase change materials with ultra-high latent heat.

We prepared a series of highly porous Polyrotaxane/sodium alginate, and Polyrotaxane/Chitosan foam alloys according to a sustainable pathway by using water as the only solvent. The foam alloys were further used as supporter materials for poly (ethylene glycol) (PEG) encapsulation, to fabricate shape-stable bio-based phase change materials (PCMs). The pore morphology and the internal interface between PEG and foam alloys were characterized by scanning electron microscope (SEM). Due to the good compatibility between foam alloys and PEG, the PCM performed perfect anti-leakage properties. The introduction of sodium alginate or Chitosan ensures the shape stability of the PCMs during the phase transition. The PCMs performed good cycle stability and showed ultra-high latent heat (171.6 J g−1–189.5 J g−1). Finally, we compared the typical indicators of this work with those reported in the literature, and the comparison highlighted that the present PCMs have the significant advantages: high melting enthalpy, convenient preparation and outstanding sustainability. Notably, the work provided a sustainable idea for the design of anti-leakage and shape-stable PEG-based PCMs.pre-print1327 K

PACIAE 2.0: An updated parton and hadron cascade model (program) for the relativistic nuclear collisions

We have updated the parton and hadron cascade model PACIAE for the relativistic nuclear collisions, from based on JETSET 6.4 and PYTHIA 5.7 to based on PYTHIA 6.4, and renamed as PACIAE 2.0. The main physics concerning the stages of the parton initiation, parton rescattering, hadronization, and hadron rescattering were discussed. The structures of the programs were briefly explained. In addition, some calculated examples were compared with the experimental data. It turns out that this model (program) works well.Comment: 23 pages, 7 figure

Shape-stable and smart polyrotaxane-based phase change materials with enhanced flexibility and fire-safety.

post-print2142 K

Distributed fiber optic monitoring of a CFA pile with a central reinforcement bar bundle

Abstract: In this paper, we present an application of distributed fiber optic sensor (DFOS) technology to measure the strain of a continuous flight auger (CFA) test pile with a central reinforcement bar bundle, during a static load test carried out in London. Being distributed in nature, DFOS gives much more information about the pile performance as compared to traditional point sensors, such as identifying cross-sectional irregularities or other anomalies. The strain profiles recorded along the depth of the piles from the DFOS were used to calculate pile deformation (contraction), shaft friction, and tip resistance under various loads. Based on this pile load test, a finite element (FE) analysis was performed using a one-dimensional nonlinear load-transfer model. Calibrated by the shaft friction and tip resistance derived from the monitored data, the FE model was able to simulate the pile and soil performance during the load testing with good accuracy. The effect of the reinforcement cage and central reinforcement bar bundle were investigated, and it was found that the addition of a reinforcement cage would reduce the pile settlement by up to 20%

Ethyl 4-acetamido-3-acet­oxy-2-benzyl-3-methyl­butano­ate

The crystal structure of the title compound, C18H25NO5, is stabilized by inter­molecular N—H⋯O hydrogen bonds, which form inversion dimers. The ethyl group is disordered over two positions in a 0.651 (12):0.349 (12) ratio

Compact groups from the Millennium Simulations: I. Their Nature and the completeness of the Hickson sample

We identify compact groups of galaxies (CGs) within mock galaxy catalogues from the Millennium Simulation at z=0 with three semi-analytic models (SAMs) of galaxy formation. CGs are identified using the same 2D criteria as those visually applied by Hickson (1982) to his CGs (HCGs), but with a brightest galaxy magnitude limit, and the blending of close projected pairs. Half of the mock CGs identified in projection contain at least 4 accordant velocities (mvCGs), versus 70% for HCGs. In comparison to mvCGs, the HCGs are only 8% complete at distances < 9000 km/s, missing the CGs with small angular sizes, a strongly dominant galaxy, and (for one SAM) the CGs that are fainter and those with lower surface brightness. We explore different ways to determine the fraction of physically dense groups. Binding energy criteria turn out to be inapplicable given the segregation between galaxies and dark matter particles. We rely instead on the combination of the 3D length of the CGs (maximum real space galaxy separation) and their elongation along the line-of-sight (ratio of maximum line-of-sight to maximum projected separations), restricting ourselves in both cases to smallest quartets within the CGs. We find that between 64% and 80% (depending on the SAM) of the mvCGs have 3D lengths shorter than 200 kpc/h, between 71% and 80% have line-of-sight elongations less than 2, while between 59% and 76% have either 3D lengths shorter than 100 kpc/h or both lengths shorter than 200 kpc/h and elongations smaller than 2. Therefore, chance alignments (CAs) of galaxies concern at most 40% of the mvCGs. These CAs are mostly produced from larger host groups, but a few have galaxies extending a few Mpc beyond the host group. The mvCGs built with the Hickson selection with (without) the close projected pair blending criterion have 10% higher (lower) fractions of physically dense systems.Comment: 19 pages, 10 figures. Accepted for publication in MNRAS. The new version has a different title and a different author list. The analysis of the nature of compact groups has been thoroughly modified, while the subsections on compact group properties and correlations have been omitted and should appear elsewher

The association between serum complement C3a and severity in patients with community-acquired pneumonia

BackgroundA few studies found that the complement system may be involved in the onset and progression of community-acquired pneumonia (CAP). However, the role of the complement system in CAP was obscure. The goal of this study was to analyze the association of serum complement C3a with CAP severity scores based on a cross-sectional study.MethodsAll 190 CAP patients and 95 control subjects were enrolled. Demographic information and clinical data were extracted. Peripheral blood samples were collected on admission.ResultsSerum complement C3a on admission was elevated in CAP patients compared with healthy subjects. The level of complement C3a was gradually elevated in parallel with CAP severity scores (CURB-65, CRB-65, PSI, SMART-COP, and CURXO). Complement C3a was positively correlated with blood routine parameters, renal function markers, and inflammatory cytokines in CAP patients. Furthermore, multivariate linear and logistic regression models found that serum complement C3a on admission was positively associated with CAP severity scores. Mechanistic research suggested that complement system inhibition alleviated Streptococcus pneumoniae-induced upregulation of IL-1β, TNF-α, IL-6, and CRP in MLE-12 cells.ConclusionsSerum complement C3a on admission is positively associated with the severity of CAP patients. Inhibiting complement system attenuates S. pneumoniae-elevated secretion of inflammatory cytokines in pulmonary epithelial cells, indicating that complement C3a is involved in the pathophysiology of CAP. Serum complement C3a may serve as an earlier diagnostic biomarker for CAP

Study of the a_0(980) meson via the radiative decay phi->eta pi^0 gamma with the KLOE detector

We have studied the phi->a_0(980) gamma process with the KLOE detector at the Frascati phi-factory DAPhNE by detecting the phi->eta pi^0 gamma decays in the final states with eta->gamma gamma and eta->pi^+ pi^- pi^0. We have measured the branching ratios for both final states: Br(phi->eta pi^0 gamma)=(7.01 +/- 0.10 +/- 0.20)x10^-5 and (7.12 +/- 0.13 +/- 0.22)x10^-5 respectively. We have also extracted the a_0(980) mass and its couplings to eta pi^0, K^+ K^-, and to the phi meson from the fit of the eta pi^0 invariant mass distributions using different phenomenological models.Comment: 17 pages, 6 figures, submitted to Physics Letters B. Corrected typos in eq.