Crystallographic study on oligonucleotide coiled-coils

En la presente tesis doctoral se han realizado estudios estructurales de DNA. Estudios previos han demostrado que los coiled-coils de d(ATATATATATAT) y d(ATATATATAT) tienen unos parámetros geométricos muy diferentes. El objetivo de esta tesis es aclarar las propiedades de los coiled-coils.Con esta finalidad se han estudiado por cristalografía de Rayos X oligonucleótidos con diferentes secuencias y con extremos cohesivos que fijen la geometría de los coiled-coils. Se han utilizado oligonucleótidos con la secuencia d(CG)n(AT)m o (AT)m(CG)n y otros semejantes. En la mayor parte de ellos n=1 y m>1, con lo que el extremo cohesivo es normalmente la secuencia CG. La estructura (a una resolución de 3.1 Å) de los cristales generados por la secuencia d(CGATATATATAT) ha sido resuelta y publicada (De Luchi et al., ChemBiochem 2006, 7, 585-587). La estructura es isomorfa con la estructura de d(AT)6 y los enlaces a puente de hidrogeno entre las bases A y T son de tipo Hoogsteen, como en el caso de la secuencia d(ATATAT). Se han obtenido diferentes tipos de coiled-coils y se han estudiado sus características y propiedades.Hemos analizado las propiedades geométricas de los coiled coils: hemos visto que los parámetros que determinan el numero de oligonucleótidos por vuelta son el "kink angle" θ, y el ángulo de torsión τ. Ha sido estudiada la relación entre estos parámetro, en función del numero de oligonucleótidos por vuelta N y el ángulo de inclinación β del coiled-coil.Hemos intentado determinar si la formación de apareamientos de tipo Hoogsteen puede influir en la geometría de los coiled-coils, los resultados sugieren que los puentes de hidrogeno de tipo Hoosteeen favorecen la formación de coiled-coils, mientras los enlaces de tipo Watson-Crick generan mas fácilmente estructuras estándar de DNA pseudocontinuas. La secuencia d(CGATATGCATAT) genera columnas tradicionales de DNA, las bases centrales G y C, apareándose con enlaces Watson-Crick fuerzan las bases ATs a aparearse de la misma manera, generando así una hélice recta pseudocontinua.Como complemento de estos estudios se han obtenido las curvas de fusión de oligonucleótidos ricos en AT, los resultados, que incluyen una formula que permite un calculo aproximado de la temperatura de fusión de secuencias cortas de DNA, han sido publicados (De Luchi et al., Analytical Biochemistry, 2003, 322, 279-282).También he tenido la oportunidad de refinar la estructura de d(TAGG) en complejo con un derivado antraquinonico que ya había sido resuelta en nuestro laboratorio. Inesperadamente, encontramos que en esta estructura no se forman G-cuádruplex como fue descrito en solución por Kettani et al, las moléculas de fármaco, a través de interacciones de stacking forman un retículo de columnas perpendiculares una a la otra, estabilizado en los "crossing points" por las flexibles y cortas moléculas de DNA. La flexibilidad del DNA gracias a sus siete ángulos de torsión, le permite adoptar una conformación tal que se adapta a la estructura creada por las columnas de fármaco. La capacidad de formar diferentes tipos de enlaces a puente de hidrogeno estabiliza su conformación en este caso no canónica. En esta estructura hemos encontrado los siguientes tipos de enlaces a puente de hidrogeno: estándar Watson-Crick, reverse Watson-Crick, interacciones simétricas Guanina-Guanina

Sífilis congênita precoce: relato de caso.

Trabalho de Conclusão de Curso - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Departamento de Pediatria, Curso de Medicina, Florianópolis, 200

LO SVILUPPO PROFESSIONALE DELL’EDUCATORE LINGUISTICO INCLUSIVO ATTRAVERSO L’OSSERVAZIONE E L’AUTO-OSSERVAZIONE: UNO STRUMENTO A PORTATA DI SGUARDO

Questo contributo intende mettere l’accento sul ruolo dell’osservazione come strumento chiave per la formazione dell’educatore linguistico inclusivo inteso come professionista riflessivo. Dopo una breve disamina dell’importanza dell’osservazione nella formazione docente e della sua percezione da parte degli insegnanti, viene introdotto il termine “osservazione”, messo in relazione con il contesto scolastico italiano e agganciato alla dimensione della riflessione, attraverso una rassegna della letteratura in materia. Si passa quindi ad approfondire la dimensione osservativa, intesa come competenza dell’insegnante che si esplica non solo verso l’esterno ma anche (e forse soprattutto) in una pratica di auto-osservazione che diventa spinta circostanziata verso un’azione didattica efficace. Il contributo si conclude con una proposta di percorso (auto)formativo incentrato sulla riflessione e la decostruzione.   The professional development of the inclusive language educator through observation and self-observation: a tool within sight This contribution aims to emphasize the role of observation as a key tool for the training of the inclusive language educator seen as a reflective professional. After a brief examination of the importance of observation in teacher training and its perception by teachers, the term “observation” is introduced, related to the Italian school context and linked to the dimension of reflection, through a review of the literature on the subject. We then move on to deepen the observational dimension, seen as the competence of the teacher expressed not only towards the outside but also (and perhaps above all) in a practice of self-observation that becomes a detailed push towards an effective didactic action. The contribution concludes with a proposal for a (self-)training path focused on reflection and deconstruction

Syn-kinematic magma ascent and batholith inflation (Sierra de San Luis/Argentina)

The measurement of the anisotropy of the magnetic susceptibility (AMS) is now routinely used since more than four decades in the analyses of rock fabrics in granitic rocks (e.g. Stacy 1960, Henry 1975, Gleizes et al. 1993). Even though the intensity of fabrics in granitoids is often weakly developed the significance of orientation and shape of crystals is the same like in other deformed rock types. By revealing the distribution of fabrics in plutonic rocks one of the still ongoing discussions in granite tectonics may be addressed: How did those sometimes voluminous batholiths were inflated in the middle crust? We are presenting magnetic fabric data on a series of Devonian batholiths that intruded the polyphase deformed metaclastites of the Sierra de San Luis (32°100– 33°200 S / 65°150 – 66°200 W) in central Argentina. Regional considerations on the tectonic regime during the emplacement of the batholiths are inferred from combined field, microstructural and AMS observations...conferenc

Comparative study of several sinusitis experimental modelling techniques in rabbits

Experimental models for clinical studies of rhinosinusitis are needed. AIM: to define a reliable, solid and reproducible experimental model for inflammatory rhinosinusitis with no innoculation of infectious agents in rabbits. Study design: Experimental. MATERIAL AND METHOD: Twenty 20 rabbits were divided into 4 groups submitted to 4 different interventions: the placement of a unilateral nasal fossa sponge, unilateral obliteration of the nasal ostium with cyanoacrylate, unilateral placement of antigens in the maxillary cavity and unilateral placement of blood in the maxillary cavity. The animals were monitored for 15 days and then anesthetized and sacrificed; the maxillary sinuses were evaluated histologicaly and results were compared with controls and between the intervention groups. CONCLUSION: Sponje and glue as agents of meatal obstruction and toxoid aplied in the antrum are efficient as methods for rhinosinusitis modeling. Blood was not efficient in producing sinusitis.A criação de modelos de estudo das rinossinusites é necessária para compreensão de seus aspectos fisiopatológicos. OBJETIVO: Sugerimos neste estudo estabelecer um modelo experimental fidedigno, reproduzível e consistente para a rinossinusite inflamatória sem uso de inoculação de agentes infecciosos em coelhos. Tipo de Estudo: Experimental. MATERIAL E MÉTODO: Foram utilizados 20 coelhos neste estudo. Os animais foram submetidos a 4 intervenções diferentes: colocação de tampão de esponja unilateral, obliteração unilateral de óstio nasal com cianoacrilato, instilação unilateral de antígenos em seio maxilar e instilação de sangue em cavidade maxilar unilateral. Os animais foram acompanhados até 15 dias do início do estudo e ao final do período de seguimento foram anestesiados e sacrificados. Os seios maxilares foram avaliados histologicamente e os resultados comparados com os seios maxilares contralaterais para controle e entre os grupos de intervenção. RESULTADOS: Todos os animais do estudo desenvolveram rinorréia amarelada unilateral até o 15º dia de acompanhamento. Apenas os animais que receberam sangue não apresentaram alterações histológicas compatíveis com rinossinusite purulenta. CONCLUSÕES: O uso de esponja e cola para obstrução do óstio meatal e a aplicação de toxóide são eficientes como produtores de modelo de rinossinusite. A aplicação de sangue no antro não produz os mesmos resultados.Santa Casa de São Paulo Depto. de OtorrinolaringologiaSanta Casa de São Paulo programa de Pós-GraduaçãoSanta Casa de São PauloUNIFESPUNIFESPSciEL

Did Patagonia collide with Gondwana in the Late Paleozoic? Some insights from a multidisciplinary study of magmatic units of the North Patagonian Massif

The origin of Patagonia and its relations with the South American crustal blocks to the north have been a matter of debate for decades. We report results from a multidisciplinary study centered on Paleozoic granitoids exposed in the northeastern corner of the North Patagonian Massif. Microstructural and magnetofabric studies reveal two suites of granitoids. Late Carboniferous (?) granitoids (Yaminué Complex, Tardugno Granodiorite, Cabeza de Vaca leucogranite) were emplaced and subsequently deformed in a major NNE-SSW compressive stress regime that also provoked top-to-the-SW thrust deformation in shallow crustal levels. Gravity and geobarometric studies show that the same major deformation event has been recorded at different crustal levels. The age and type of deformation of this event recorded across the northern boundary of Patagonia strongly supports a Late Carboniferous – Early Permian frontal collision between Patagonia and Gondwana. This major deformation event ceased by 281 Ma when the Navarrete Plutonic Complex, which shows mainly magmatic fabrics, was emplaced under a far-field WNW-ESE stress regime. Crustal continuity between the North Patagonian Massif and the Pampia and Arequipa- Antofalla terranes is suggested by similar Late Paleoproterozoic crustal model ages, comparable detrital zircon ages in Early Paleozoic successions, the apparent continuity of an Early Ordovician continental magmatic arc and paleomagnetic data. Reconciliation of this evidence with the Late Paleozoic frontal collision is obtained in a tectonic model that suggests that the North Patagonian Massif is a parautochthonous crustal block

The Conlara metamorphic complex: Lithology, provenance, metamorphic constraints on the metabasic rocks, and chime monazite dating

The Conlara Metamorphic Complex, the easternmost complex of the Sierra de San Luis, is a key unit to understand the relationship between the late Proterozoic-Early Cambrian Pampean and the Upper Cambrian-Middle Ordovician Famatinian orogenies of the Eastern Sierras Pampeanas. The Conlara Metamorphic Complex extends to the east to the foothills of the Sierra de Comechingones and to the west up the Río Guzmán shear zone. The main rock types of the CMC are metaclastic and metaigneous rocks that are intruded by Ordovician and Devonian granitoids. The metaclastic units comprise fine to medium-grained metagreywackes and scarce metapelites with lesser amounts of tourmaline schists and tourmalinites whereas the metaigneous rocks encompass basic and granitoids rocks. The former occur as rare amphibolite interlayered within the metasedimentary rocks. The granitic component corresponds to a series of orthogneisses and migmatites (stromatite and diatexite). The CMC is divided in four groups based on the dominant lithological associations: San Martin and La Cocha correspond mainly to schists and some gneisses and Santa Rosa and San Felipe encompass mainly paragneisses, migmatites and orthogneisses. The Conlara Metamoprphic Complex underwent a polyphase metamorphic evolution. The penetrative D2-S2 foliation was affected by upright, generally isoclinal, N-NE trending D3 folds that control the NNE outcrop patterns of the different groups. An earlier, relic S1 is preserved in microlithons. Discontinuous high-T shear zones within the schists and migmatites are related with D4 whereas some fine-grained discontinuous shear bands attest for a D5 deformation phase. Geochemistry of both non-migmatitic metaclastic units and amphibolites suggest that the Conlara Metamorphic Complex represents an arc related basin. Maximun depositional ages indicate a pre- 570 Ma deposition of the sediments. An ample interval between sedimentation and granite emplacement in the already metamorphic complex is indicated by the 497 ± 8 Ma age of El Peñon granite. D1-D2 history took place at 564 ± 21 Ma as indicated by one PbSL age calculated for the M2 garnet of La Cocha Group. D3 is constrained by the pervasively solid-state deformed Early Ordovician granitoids which exhibits folded xenoliths of the D1-D2 deformed metaclastic rocks. Pressure-temperature pseudosections were calculated for one amphibolite using the geologically realistic system MnNCKFMASHTO (MnO–Na2O–CaO–K2O–FeO–MgO–Al2O3–SiO2–H2O–TiO2–Fe2O3). Peak metamophic conditions (M2) indicate 6 kbar and 620 °C. Late chlorite on the rims and in cracks of garnet, along with titanite rims on ilmenite and matrix plagioclase breaking down to albite suggests that the P-T path moved back down. Monazite analyses yield isochron Th–U–Pb ages ranging from 446 to 418 Ma. The oldest age of 446 ± 5 Ma correspond to a migmatite from the Santa Rosa Group. Monazites in samples from the La Cocha and the San Martin group crystallized at decreasing temperatures, followed by the 418 ± 10 Ma low-Y2O3 monazites in one sample of the la Cocha Group that was also obtained from a migmatite, and would likely mark a later stage of a retrograde metamorphism New CHIME monazite ages presented here likely represent post-peak fluid assisted recrystallization that are similar to amphibole and muscovite cooling ages. Therefore the monazite ages may represent a re-equilibration of the monazite on the cooling path of the basement complex.Fil: López de Luchi, Mónica G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Geocronología y Geología Isotópica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Geocronología y Geología Isotópica; ArgentinaFil: Martínez Dopico, Carmen Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Geocronología y Geología Isotópica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Geocronología y Geología Isotópica; ArgentinaFil: Cutts, Kathryn Ann. Universidade do Estado de Rio do Janeiro; BrasilFil: Schulz, Bernhard. Institute of Mineralogy; AlemaniaFil: Siegesmund, Siegfried. Universität Göttingen; AlemaniaFil: Wemmer, Klaus. Universität Göttingen; AlemaniaFil: Montenegro, Teresita Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Geociencias Básicas, Aplicadas y Ambientales de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Geociencias Básicas, Aplicadas y Ambientales de Buenos Aires; Argentin

ETNOGRAFIAS EM TEMPOS DE PANDEMIA

Apresentação do dossiê Etnografia em tempos de pandemia

Geochronology of quarz-monzodioritic to tonalitic Stock Musters, an Ordovician intrusive from Valcheta, Northpatagonian Massif

Esta contribución informa la primera edad de cristalización U-Pb en circón de un stock monzodiorítico cuarzoso a tonalítico, aquí denominado formalmente como Diorita Musters, y perteneciente al conjunto de granitoides asociados al plutón leucogranitíco Valchetaque forma parte del conjunto de granitoides de edad ordovícica temprana aflorantes en el sector nororiental del Macizo Norpatagónico(Complejo plutónico Punta Sierra, Busteros et al. 1998). Los resultados de los análisis U-Pb en circones revelan una edad de cristalización ordovícica inferior (470 ± 2 Ma; MSWD =0.99) para este pulso magmático, confirmando la extensión de este magmatismoordovícico por más de 200 km2 y afirmando su importancia regional y conexión con la orogenia Famatiniana.This contribution reports the first U-Pb age in zircons of a quartz-monzodioritic to tonalitic stock, here formally denominated as Musters Diorite and belonging to the group of Early Ordovician granitoids outcropping in the northeastern sector of the Northern Patagonian Massif (Punta Sierra Plutonic Complex, Busteros et al. 1998). Our results of U-Pb analyses in zircons reveal an Early Ordovician crystallization age (470 ± 2 Ma; MSWD =0.99; N=70/89) for this magmatic pulse, confirming the extension of this magmatism for more than 200 km2 and showing its regional importance and connection with the Famatinian OrogenyFil: Grillo Vidal, Carolina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Geocronología y Geología Isotópica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Geocronología y Geología Isotópica; Argentina. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ciencias Geológicas. Area de Petrologia; ArgentinaFil: Martínez Dopico, Carmen Irene. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ciencias Geológicas. Area de Petrologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Geocronología y Geología Isotópica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Geocronología y Geología Isotópica; ArgentinaFil: López de Luchi, Mónica G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Geocronología y Geología Isotópica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Geocronología y Geología Isotópica; Argentin