496 research outputs found
Preparation of Îș-Casein by Gel Filtration
Abstract A procedure for the preparation of pure Îș-casein by Sephadex gel filtration is described. Unreduced casein is filtered through a 2.5- by 95-cm column of Sephadex G-150 equilibrated with 0.005m triscitrate buffer, pH 8.6, containing 6 m urea. Îș-Casein is excluded from the gel and emerges as a sharp peak at the void volume; all other caseins are retarded by the gel. The method is particularly suitable for preparing pure Îș-casein in good yield from small quantities of whole casein, but larger amounts of Îș-casein are readily prepared. The method is also suitable for the purification of Îș-casein-rich preparations
Underdetermined instantaneous audio source separation via local Gaussian modeling
International audienceUnderdetermined source separation is often carried out by modeling time-frequency source coefficients via a fixed sparse prior. This approach fails when the number of active sources in one time-frequency bin is larger than the number of channels or when active sources lie on both sides of an inactive source. In this article, we partially address these issues by modeling time-frequency source coefficients via Gaussian priors with free variances. We study the resulting maximum likelihood criterion and derive a fast non-iterative optimization algorithm that finds the global minimum. We show that this algorithm outperforms state-of-the- art approaches over stereo instantaneous speech mixtures
The Possibilist Transactional Interpretation and Relativity
A recent ontological variant of Cramer's Transactional Interpretation, called
"Possibilist Transactional Interpretation" or PTI, is extended to the
relativistic domain. The present interpretation clarifies the concept of
'absorption,' which plays a crucial role in TI (and in PTI). In particular, in
the relativistic domain, coupling amplitudes between fields are interpreted as
amplitudes for the generation of confirmation waves (CW) by a potential
absorber in response to offer waves (OW), whereas in the nonrelativistic
context CW are taken as generated with certainty. It is pointed out that
solving the measurement problem requires venturing into the relativistic domain
in which emissions and absorptions take place; nonrelativistic quantum
mechanics only applies to quanta considered as 'already in existence' (i.e.,
'free quanta'), and therefore cannot fully account for the phenomenon of
measurement, in which quanta are tied to sources and sinks.Comment: Final version with some minor corrections as published in Foundations
of Physics. This paper has significant overlap with Chapter 6 of my book on
the Transactional Interpretation, forthcoming from Cambridge University
Press:
http://www.cambridge.org/us/knowledge/isbn/item6860644/?site_locale=en_US
(Additional preview material is available at rekastner.wordpress.com)
Comments welcom
Out-of-equilibrium evolution of scalar fields in FRW cosmology: renormalization and numerical simulations
We present a renormalized computational framework for the evolution of a
self-interacting scalar field (inflaton) and its quantum fluctuations in an FRW
background geometry. We include a coupling of the field to the Ricci scalar
with a general coupling parameter . We take into account the classical and
quantum back reactions, i.e., we consider the the dynamical evolution of the
cosmic scale factor. We perform, in the one-loop and in the large-N
approximation, the renormalization of the equation of motion for the inflaton
field, and of its energy momentum tensor. Our formalism is based on a
perturbative expansion for the mode functions, and uses dimensional
regularization. The renormalization procedure is manifestly covariant and the
counter terms are independent of the initial state. Some shortcomings in the
renormalization of the energy-momentum tensor in an earlier publication are
corrected. We avoid the occurence of initial singularities by constructing a
suitable class of initial states. The formalism is implemented numerically and
we present some results for the evolution in the post-inflationary preheating
era.Comment: 44 pages, uses latexsym, 6 pages with 11 figures in a .ps fil
Superdeformed rotational bands in the Mercury region; A Cranked Skyrme-Hartree-Fock-Bogoliubov study
A study of rotational properties of the ground superdeformed bands in \Hg{0},
\Hg{2}, \Hg{4}, and \Pb{4} is presented. We use the cranked
Hartree-Fock-Bogoliubov method with the {\skm} parametrization of the Skyrme
force in the particle-hole channel and a seniority interaction in the pairing
channel. An approximate particle number projection is performed by means of the
Lipkin-Nogami prescription. We analyze the proton and neutron quasiparticle
routhians in connection with the present information on about thirty presently
observed superdeformed bands in nuclei close neighbours of \Hg{2}.Comment: 26 LaTeX pages, 14 uuencoded postscript figures included, Preprint
IPN-TH 93-6
Targeting the Ataxia Telangiectasia Mutated-null Phenotype in Chronic Lymphocytic Leukemia with Pro-oxidants
Inactivation of the Ataxia Telangiectasia Mutated gene in chronic lymphocytic leukemia results in resistance to p53-dependent apoptosis and inferior responses to treatment with DNA damaging agents. Hence, p53-independent strategies are required to target Ataxia
Telangiectasia Mutated-deficient chronic lymphocytic leukemia. As Ataxia Telangiectasia Mutated has been implicated in redox homeostasis, we investigated the effect of the Ataxia Telangiectasia Mutated-null chronic lymphocytic leukemia genotype on cellular responses to
oxidative stress with a view to therapeutic targeting. We found that in comparison to Ataxia Telangiectasia Mutated-wild type chronic lymphocytic leukemia, pro-oxidant treatment of Ataxia Telangiectasia Mutated-null cells led to reduced binding of NF-E2 p45-related factor-2 to antioxidant response elements and thus decreased expression of target genes. Furthermore, Ataxia Telangiectasia Mutated-null chronic lymphocytic leukemia cells contained lower levels of antioxidants and elevated mitochondrial reactive oxygen species. Consequently, Ataxia Telangiectasia Mutated-null chronic lymphocytic leukemia, but not tumours with 11q deletion or TP53 mutations, exhibited differentially increased sensitivity to pro-oxidants both in vitro and in vivo. We found that cell death was mediated by a p53- and caspase-independent mechanism associated with apoptosis inducing factor activity. Together, these data suggest that defective redox-homeostasis represents an attractive therapeutic target for Ataxia Telangiectasia Mutated-null chronic lymphocytic leukemia
Development and external validation study of a melanoma risk prediction model incorporating clinically assessed naevi and solar lentigines
Background:
Melanoma risk prediction models could be useful for matching preventive interventions to patientsâ risk.
Objectives:
To develop and validate a model for incident firstâprimary cutaneous melanoma using clinically assessed risk factors.
Methods:
We used unconditional logistic regression with backward selection from the Australian Melanoma Family Study (461 cases and 329 controls) in which age, sex and city of recruitment were kept in each step, and we externally validated it using the Leeds Melanoma CaseâControl Study (960 cases and 513 controls). Candidate predictors included clinically assessed wholeâbody naevi and solar lentigines, and selfâassessed pigmentation phenotype, sun exposure, family history and history of keratinocyte cancer. We evaluated the predictive strength and discrimination of the model risk factors using odds per ageâ and sexâadjusted SD (OPERA) and the area under curve (AUC), and calibration using the HosmerâLemeshow test.
Results:
The final model included the number of naevi â„ 2 mm in diameter on the whole body, solar lentigines on the upper back (a sixâlevel scale), hair colour at age 18 years and personal history of keratinocyte cancer. Naevi was the strongest risk factor; the OPERA was 3·51 [95% confidence interval (CI) 2·71â4·54] in the Australian study and 2·56 (95% CI 2·23â2·95) in the Leeds study. The AUC was 0·79 (95% CI 0·76â0·83) in the Australian study and 0·73 (95% CI 0·70â0·75) in the Leeds study. The HosmerâLemeshow test Pâvalue was 0·30 in the Australian study and < 0·001 in the Leeds study.
Conclusions:
This model had good discrimination and could be used by clinicians to stratify patients by melanoma risk for the targeting of preventive interventions.
What's already known about this topic?
Melanoma risk prediction models may be useful in prevention by tailoring interventions to personalized risk levels.
For reasons of feasibility, time and cost many melanoma prediction models use selfâassessed risk factors. However, individuals tend to underestimate their naevus numbers.
What does this study add?
We present a melanoma risk prediction model, which includes clinicallyâassessed wholeâbody naevi and solar lentigines, and selfâassessed risk factors including pigmentation phenotype and history of keratinocyte cancer.
This model performs well on discrimination, the model's ability to distinguish between individuals with and without melanoma, and may assist clinicians to stratify patients by melanoma risk for targeted preventive interventions
HI in the Outskirts of Nearby Galaxies
The HI in disk galaxies frequently extends beyond the optical image, and can
trace the dark matter there. I briefly highlight the history of high spatial
resolution HI imaging, the contribution it made to the dark matter problem, and
the current tension between several dynamical methods to break the disk-halo
degeneracy. I then turn to the flaring problem, which could in principle probe
the shape of the dark halo. Instead, however, a lot of attention is now devoted
to understanding the role of gas accretion via galactic fountains. The current
cold dark matter theory has problems on galactic scales, such as
the core-cusp problem, which can be addressed with HI observations of dwarf
galaxies. For a similar range in rotation velocities, galaxies of type Sd have
thin disks, while those of type Im are much thicker. After a few comments on
modified Newtonian dynamics and on irregular galaxies, I close with statistics
on the HI extent of galaxies.Comment: 38 pages, 17 figures, invited review, book chapter in "Outskirts of
Galaxies", Eds. J. H. Knapen, J. C. Lee and A. Gil de Paz, Astrophysics and
Space Science Library, Springer, in pres
Dimensionless cosmology
Although it is well known that any consideration of the variations of
fundamental constants should be restricted to their dimensionless combinations,
the literature on variations of the gravitational constant is entirely
dimensionful. To illustrate applications of this to cosmology, we explicitly
give a dimensionless version of the parameters of the standard cosmological
model, and describe the physics of Big Bang Neucleosynthesis and recombination
in a dimensionless manner. The issue that appears to have been missed in many
studies is that in cosmology the strength of gravity is bound up in the
cosmological equations, and the epoch at which we live is a crucial part of the
model. We argue that it is useful to consider the hypothetical situation of
communicating with another civilization (with entirely different units),
comparing only dimensionless constants, in order to decide if we live in a
Universe governed by precisely the same physical laws. In this thought
experiment, we would also have to compare epochs, which can be defined by
giving the value of any {\it one} of the evolving cosmological parameters. By
setting things up carefully in this way one can avoid inconsistent results when
considering variable constants, caused by effectively fixing more than one
parameter today. We show examples of this effect by considering microwave
background anisotropies, being careful to maintain dimensionlessness
throughout. We present Fisher matrix calculations to estimate how well the fine
structure constants for electromagnetism and gravity can be determined with
future microwave background experiments. We highlight how one can be misled by
simply adding to the usual cosmological parameter set
'Education, education, education' : legal, moral and clinical
This article brings together Professor Donald Nicolson's intellectual interest in professional legal ethics and his long-standing involvement with law clinics both as an advisor at the University of Cape Town and Director of the University of Bristol Law Clinic and the University of Strathclyde Law Clinic. In this article he looks at how legal education may help start this process of character development, arguing that the best means is through student involvement in voluntary law clinics. And here he builds upon his recent article which argues for voluntary, community service oriented law clinics over those which emphasise the education of students
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