55 research outputs found
Cation Discrimination in Organic Electrochemical Transistors by Dual Frequency Sensing
In this work, we propose a strategy to sense quantitatively and specifically
cations, out of a single organic electrochemical transistor (OECT) device
exposed to an electrolyte. From the systematic study of six different chloride
salts over 12 different concentrations, we demonstrate that the impedance of
the OECT device is governed by either the channel dedoping at low frequency and
the electrolyte gate capacitive coupling at high frequency. Specific cationic
signatures, which originates from the different impact of the cations behavior
on the poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS)
polymer and their conductivity in water, allow their discrimination at the same
molar concentrations. Dynamic analysis of the device impedance at different
frequencies could allow the identification of specific ionic flows which could
be of a great use in bioelectronics to further interpret complex mechanisms in
biological media such as in the brain.Comment: Full text and supporting informatio
Mitochondrial Uncoupling Protein-2 (UCP2) Mediates Leptin Protection Against MPP+ Toxicity in Neuronal Cells
Mitochondrial dysfunction is involved in the pathogenesis of neurodegenerative diseases, including Parkinsonâs disease (PD). Uncoupling proteins (UCPs) delink ATP production from biofuel oxidation in mitochondria to reduce oxidative stress. UCP2 is expressed in brain, and has neuroprotective effects under various toxic insults. We observed induction of UCP2 expression by leptin in neuronal cultures, and hypothesize that leptin may preserve neuronal survival via UCP2. We showed that leptin preserved cell survival in neuronal SH-SY5Y cells against MPP+ toxicity (widely used in experimental Parkinsonian models) by maintaining ATP levels and mitochondrial membrane potential (MMP); these effects were accompanied by increased UCP2 expression. Leptin had no effect in modulating reactive oxygen species levels. Stable knockdown of UCP2 expression reduced ATP levels, and abolished leptin protection against MPP+-induced mitochondrial depolarization, ATP deficiency, and cell death, indicating that UCP2 is critical in mediating these neuroprotective effects of leptin against MPP+ toxicity. Interestingly, UCP2 knockdown increased UCP4 expression, but not of UCP5. Our findings show that leptin preserves cell survival by maintaining MMP and ATP levels mediated through UCP2 in MPP+-induced toxicity
Palaeogenomics of Upper Palaeolithic to Neolithic European hunter-gatherers
: Modern humans have populated Europe for more than 45,000 years1,2. Our knowledge of the genetic relatedness and structure of ancient hunter-gatherers is however limited, owing to the scarceness and poor molecular preservation of human remains from that period3. Here we analyse 356 ancient hunter-gatherer genomes, including new genomic data for 116 individuals from 14 countries in western and central Eurasia, spanning between 35,000 and 5,000 years ago. We identify a genetic ancestry profile in individuals associated with Upper Palaeolithic Gravettian assemblages from western Europe that is distinct from contemporaneous groups related to this archaeological culture in central and southern Europe4, but resembles that of preceding individuals associated with the Aurignacian culture. This ancestry profile survived during the Last Glacial Maximum (25,000 to 19,000 years ago) in human populations from southwestern Europe associated with the Solutrean culture, and with the following Magdalenian culture that re-expanded northeastward after the Last Glacial Maximum. Conversely, we reveal a genetic turnover in southern Europe suggesting a local replacement of human groups around the time of the Last Glacial Maximum, accompanied by a north-to-south dispersal of populations associated with the Epigravettian culture. From at least 14,000 years ago, an ancestry related to this culture spread from the south across the rest of Europe, largely replacing the Magdalenian-associated gene pool. After a period of limited admixture that spanned the beginning of the Mesolithic, we find genetic interactions between western and eastern European hunter-gatherers, who were also characterized by marked differences in phenotypically relevant variants
Palaeogenomics of Upper Palaeolithic to Neolithic European hunter-gatherers
Publisher Copyright: © 2023, The Author(s).Modern humans have populated Europe for more than 45,000 years1,2. Our knowledge of the genetic relatedness and structure of ancient hunter-gatherers is however limited, owing to the scarceness and poor molecular preservation of human remains from that period3. Here we analyse 356 ancient hunter-gatherer genomes, including new genomic data for 116 individuals from 14 countries in western and central Eurasia, spanning between 35,000 and 5,000 years ago. We identify a genetic ancestry profile in individuals associated with Upper Palaeolithic Gravettian assemblages from western Europe that is distinct from contemporaneous groups related to this archaeological culture in central and southern Europe4, but resembles that of preceding individuals associated with the Aurignacian culture. This ancestry profile survived during the Last Glacial Maximum (25,000 to 19,000 years ago) in human populations from southwestern Europe associated with the Solutrean culture, and with the following Magdalenian culture that re-expanded northeastward after the Last Glacial Maximum. Conversely, we reveal a genetic turnover in southern Europe suggesting a local replacement of human groups around the time of the Last Glacial Maximum, accompanied by a north-to-south dispersal of populations associated with the Epigravettian culture. From at least 14,000 years ago, an ancestry related to this culture spread from the south across the rest of Europe, largely replacing the Magdalenian-associated gene pool. After a period of limited admixture that spanned the beginning of the Mesolithic, we find genetic interactions between western and eastern European hunter-gatherers, who were also characterized by marked differences in phenotypically relevant variants.Peer reviewe
Mudanças conceituais do desenvolvimento rural e suas influĂȘncias nas polĂticas pĂșblicas
Simultaneous Study of the Growth and Grazing Mortality Rates of Microbial Food Web Components in a Mediterranean Coastal Lagoon
The abundances of 17 cytometric groups encompassing four groups of bacteria, three groups of cyanobacteria, six groups of eukaryotic picophytoplankton (<2–3 µm), and four groups of small eukaryotic nanophytoplankton (between 3 and 6 µm) were studied in the Thau Lagoon across different seasonal conditions. Among them, the growth (µ) and mortality rates due to grazing (g) of 12 groups of bacteria, cyanobacteria, and eukaryotic pico- and nanophytoplankton were simultaneously studied in the Thau Lagoon via four dilution experiments across different seasonal conditions. The abundances of heterotrophic flagellates and ciliates and their potential predators were studied and linked to prey mortality. Bacteria were more active than phytoplankton and displayed the highest growth and grazing mortality rates. Most studied groups had g:µ ratios > 1, suggesting that predators efficiently grazed and transferred these preys. Surprisingly, the observed variations in predator abundance across seasons did not necessarily correspond with changes in grazing pressure. The significant positive relationship found between water temperature and bacterial grazing mortality rates and the reverse trend observed for larger eukaryotic prey suggested that warmer water increases small prokaryote transfer to higher trophic levels but disadvantages larger eukaryotic prey transfer
Role of protozooplankton and virioplankton on the bacterioplankton and phytoplankton control in Mediterranean coastal water.
Ce travail de thĂšse focalise sur le fonctionnement du rĂ©seau microbien de la lagune de Thau Ă travers une approche « RĂ©seau » ayant permis lâĂ©tude simultanĂ©e de lâensemble des groupes microbiens (21 groupes) des virus au microzooplancton. La croissance et la mortalitĂ© des micro-organismes ont Ă©tĂ© Ă©tudiĂ©es expĂ©rimentalement lors de plusieurs saisons puis dâune Ă©tude en mĂ©socosme. Nous avons dĂ©terminĂ© les proportions de mortalitĂ© dont sont responsables le microzooplancton et les virus au travers de la lyse virale. Un suivi in situ puis en mĂ©socosme dans la lagune de Thau nous a permis d'observer la rĂ©ponse des composantes du rĂ©seau microbien Ă un forçage typiquement MĂ©diterranĂ©en, la crue.Il apparait que les bactĂ©ries hĂ©tĂ©rotrophes ont les plus fortes croissances allant jusquâĂ 2,18 jour-1. La croissance pour divers groupes pico- et nano-phytoplanctoniques Ă©tait infĂ©rieure Ă 1,5 jour-1. La cause majeure de mortalitĂ© des diffĂ©rents groupes de micro-organismes Ă©tudiĂ©s est la prĂ©dation du microzooplancton (> 90% en moyenne), tandis que la lyse virale semble ĂȘtre un processus sporadique et nettement moins important. Le fait que les taux de prĂ©dation observĂ©s soient trĂšs proches des taux de croissance de diffĂ©rents micro-organismes suggĂšre que le rĂ©seau microbien Ă©tudiĂ© prĂ©sente une forte efficacitĂ© de transfert de la biomasse vers les niveaux trophiques supĂ©rieurs.Dans ce contexte, la crue provoque Ă court terme une diminution de la croissance microbienne et du broutage, alors quâelle dĂ©clenche la lyse virale chez certains groupes. Cependant, la rĂ©activitĂ© de ce rĂ©seau permet un rĂ©tablissement rapide indiquant une forte capacitĂ© de rĂ©silience de ce systĂšme.The aims of this thesis work were to shed new lights on the functioning of the microbial food web (MFW) in the Thau coastal lagoon through a âglobal approachâ that permit us to study simultaneously the entire MFW components (21 groups) from viruses to microzooplankton. Growth and mortality of microorganisms were studied experimentally along seasons and during a mesocosm experiment. We particularly focus on mortality due to microzooplankton (<200”m) grazing and viral lysis. Responses of the different components of the MFW under a typical Mediterranean forcing, flash flood, was also studied during an in situ monitoring and a mesocosm experiment in the Thau Lagoon.In conclusion, it appears that heterotrophic bacteria show the highest growth rates (until 2.18 day-1). Growth rates of pico- and nanophytolankton groups were always lower than 1.5 day-1. The major cause of mortality of the different group studied, reaching on average 90%, was due to the predation by the microzooplankton. Mortality due to viral lysis appeared to be a âsporadic processesâ, less important than microzooplankton grazing indeed. In addition, the observed grazing rates or bacterivory were close to growth rates of microorganisms, suggesting an efficient trophic transfer of the microbial biomass towards higher trophic levels.River flash flood; an important forcing in the Mediterranean coastal zone; triggered on a short time scale, a decrease in growth and grazing rates of microorganisms whereas viral lysis was enhanced. However, the important dynamics of this studied MFW allowed the rapid reestablishment of growth and grazing rates, indicating a strong resilience capability of this system
High Rectification Ratio in Polymer Diode Rectifier through Interface Engineering with Self-Assembled Monolayer
In this work, we demonstrate P3HT (poly 3-hexylthiophene) organic rectifier diode both in rigid and flexible substrate with a rectification ratio up to 106. This performance has been achieved through tuning the work function of gold with a self-assembled monolayer of 2,3,4,5,6-pentafluorobenzenethiol (PFBT). The diode fabricated on flexible paper substrate shows a very good electrical stability under bending tests and the frequency response is estimated at more than 20 MHz which is sufficient for radio frequency identification (RFID) applications. It is also shown that the low operating voltage of this diode can be a real advantage for use in a rectenna for energy harvesting systems. Simulations of the diode structure show that it can be used at GSM and Wi-Fi frequencies if the diode capacitance is reduced to a few pF and its series resistance to a few hundred ohms. Under these conditions, the DC voltages generated by the rectenna can reach a value up to 1 V
Concentric-Electrode Organic Electrochemical Transistors: Case Study for Selective Hydrazine Sensing
We report on hydrazine-sensing organic electrochemical transistors (OECTs) with a design consisting of concentric annular electrodes. The design engineering of these OECTs was motivated by the great potential of using OECT sensing arrays in fields such as bioelectronics. In this work, poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS)-based OECTs have been studied as aqueous sensors that are specifically sensitive to the lethal hydrazine molecule. These amperometric sensors have many relevant features for the development of hydrazine sensors, such as a sensitivity down to 10â5 M of hydrazine in water, an order of magnitude higher selectivity for hydrazine than for nine other water-soluble common analytes, the capability to entirely recover its base signal after water flushing, and a very low operation voltage. The specificity for hydrazine to be sensed by our OECTs is caused by its catalytic oxidation at the gate electrode, and enables an increase in the output current modulation of the devices. This has permitted the device-geometry study of the whole series of 80 micrometric OECT devices with sub-20-nm PEDOT:PSS layers, channel lengths down to 1 ”m, and a specific device geometry of coplanar and concentric electrodes. The numerous geometries unravel new aspects of the OECT mechanisms governing the electrochemical sensing behaviours of the deviceâmore particularly the effect of the contacts which are inherent at the micro-scale. By lowering the device cross-talk, micrometric gate-integrated radial OECTs shall contribute to the diminishing of the readout invasiveness and therefore further promote the development of OECT biosensors
Effects of experimental warming on small phytoplankton, bacteria and viruses in autumn in the Mediterranean coastal Thau Lagoon
International audienceTo investigate the responses of a natural microbial plankton community of coastal Mediterranean waters to warming, which are still poorly known, an in situ mesocosm experiment was carried out in Thau Lagoon during autumn 2018. Several microorganisms, including virio-, bacterio-, and phytoplankton \ 10 lm in size, were monitored daily and analysed using flow cytometry for 19 consecutive days in six mesocosms. Three mesocosms (control) had the same natural water temperature as the lagoon, and the other three were warmed by ? 3°C in relation to the control temperature. The cytometric analyses revealed an unexpected community dominated by picophytoplanktonic cells, including Prochlorococcus-like and Picochlorum-like cells, which had not previously been found in Thau Lagoon. The experimental warming treatment increased the abundances of nanophytoplankton, cyanobacteria, bacteria and viruses during the experiment and triggered earlier blooms of cyanobacteria and picoeukaryotes. Only the abundance of Picochlorum-like cells was significantly reduced under warmer conditions. The growth and Handling Editor: Télesphore Sime-Ngando
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