Putting an Emphasis on Local and Healthy Living

Local First is an organization located in Grand Rapids, Michigan, that works to promote a lifestyle built upon local product consumption. Local First works with a wide variety of compa-­‐ nies to achieve this goal and is not limited to one specific area of the market. Four students from Grand Valley State University have teamed up with Local First to address and improve ar-­‐ eas in need of attention. Nutrition, Diversity, and public awareness of Local First are current areas described as needing improvement by both the students and the Local First executive as-­‐ sistant. Students designed, printed, and distributed a brochure specifically focused on increas-­‐ ing public awareness of the importance of eating nutritional food, as well as how to do so through local food sources. Determining what information is most effective for the limited space a brochure offers is one of the challenge we dealt with during the early design stages of the project. Despite the initial success of the brochure, further work is needed with Local First in order to keep expanding on the importance of nutritional eating achieved locally. Future partners should focus on working with more Local First partners (restaurant owners, farmers, local market owners, etc…) in order to provide the Grand Rapids community with more options on where to obtain nutritional local food

Evaluation of two nutrient input methods of HSPF: monthly data block and manual time series

Proceedings of the Seventh International Conference on Hydroscience and Engineering, Philadelphia, PA, September 2006. http://hdl.handle.net/1860/732Use of AGCHEM modules within Hydrological Simulation Program Fortran (HSPF) requires extensive efforts of time series data preparation. In this research, two nutrient input methods, Monthly Data Block and Manual Time Series, were compared and evaluated with a developed St. Louis Bay watershed water quality model. The results indicated that HSPF responded to nutrient input very well and there was much difference in the generated nutrient loadings between these two methods. The Monthly Data Block method is easier to use but it misrepresents nutrient distribution, cannot preserve intended mass balance, and cannot simulate field fertilization practice. Monthly Data Block approach is suitable to provide nutrient inputs from atmospheric deposition. Manual Time Series method is more accurate and flexible to input nutrient from any sources, but is very time-consuming, especially for long time simulation. The users should understand the characteristics of model functions to ensure the correct input of boundary loading since correct input of nutrient boundary loadings has strong impacts on in-stream nutrient modeling

Fluorescent Labeling of Calmodulin for Future Application

Calmodulin (CaM) is a small dumbbell-shaped, intermediary protein. CaM binds with several hundred different molecules to help control bodily functions. At Northwestern, we use fluorescently labeled CaM to understand these functions further. The goal of this project is to successfully label CaM protein using the fluorescent dye Alexa Fluor 594. After labeling CaM, we will use buffer exchange chromatography to purify the sample. Following that, we will use three processes to verify the successful labeling of CaM: UV/VIS spectroscopy, mass spectroscopy, and fluorescent microscope imaging. Once labeled and purified, our CaM samples can be used for years to come by Northwestern students and professors to understand the unknown functions of bodily enzymes it interacts with

An Uncharged Amine in the Transition State of the Ribosomal Peptidyl Transfer Reaction

The ribosome has an active site comprised of RNA that catalyzes peptide bond formation. To understand how RNA promotes this reaction requires a detailed understanding of the chemical transition state. Here, we report the Brønsted coefficient of the α-amino nucleophile with a series of puromycin derivatives. Both 50S subunit- and 70S ribosome-catalyzed reactions displayed linear free-energy relationships with slopes close to zero under conditions where chemistry is rate limiting. These results indicate that, at the transition state, the nucleophile is neutral in the ribosome-catalyzed reaction, in contrast to the substantial positive charge reported for typical uncatalyzed aminolysis reactions. This suggests that the ribosomal transition state involves deprotonation to a degree commensurate with nitrogen-carbon bond formation. Such a transition state is significantly different from that of uncatalyzed aminolysis reactions in solution

Developmentally Sensitive Implementation of Core Elements of Evidence-Based Treatments: Practical Strategies for Youth With Internalizing Disorders

MANY TREATMENT APPROACHES for psychological disorders among children and adolescents are downward extensions of adult treatment models. According to Barrett (2000), when treatments for childhood disorders are based on cognitive behavioral models of adult disorders, clinicians may make inaccurate assumptions, such as viewing children as “little adults,” thereby failing to adjust treatment terminology for children and ignoring contextual factors such as families and peers. Subscribing to adult models may also result in a lack of awareness of research findings in the field of developmental psychology (e.g., cognitive abilities, social skills, emotion regulation) and, consequently, implementation of treatment strategies in a similar manner across levels of development (e.g., assuming all children possess the same level of meta-cognitive skills). As Kingery and colleagues (2006) emphasize, simply utilizing a treatment that has been developed for youth is not sufficient. Particularly when implementing manual-based CBT for youth with internalizing disorders, clinicians must be knowledgeable, creative, and flexible, taking each child’s individual cognitive, social, and emotional skills into consideration to provide the most developmentally appropriate intervention

Neuropeptide Deficient Mice Have Attenuated Nociceptive, Vascular, and Inflammatory Changes in a Tibia Fracture Model of Complex Regional Pain Syndrome

BACKGROUND: Distal limb fracture in man can induce a complex regional pain syndrome (CRPS) with pain, warmth, edema, and cutaneous inflammation. In the present study substance P (SP, Tac1(−/−)) and CGRP receptor (RAMP1(−/−)) deficient mice were used to investigate the contribution of neuropeptide signaling to CRPS-like changes in a tibia fracture mouse model. Wildtype, Tac1(−/−), and RAMP1(−/−) mice underwent tibia fracture and casting for 3 weeks, then the cast was removed and hindpaw mechanical allodynia, unweighting, warmth, and edema were tested over time. Hindpaw skin was collected at 3 weeks post-fracture for immunoassay and femurs were collected for micro-CT analysis. RESULTS: Wildtype mice developed hindpaw allodynia, unweighting, warmth, and edema at 3 weeks post-fracture, but in the Tac1(−/−) fracture mice allodynia and unweighting were attenuated and there was no warmth and edema. RAMP1(−/−) fracture mice had a similar presentation, except there was no reduction in hindpaw edema. Hindpaw skin TNFα, IL-1β, IL-6 and NGF levels were up-regulated in wildtype fracture mice at 3 weeks post-fracture, but in the Tac1(−/−) and RAMP1(−/−) fracture mice only IL-6 was increased. The epidermal keratinocytes were the cellular source for these inflammatory mediators. An IL-6 receptor antagonist partially reversed post-fracture pain behaviors in wildtype mice. CONCLUSIONS: In conclusion, both SP and CGRP are critical neuropeptide mediators for the pain behaviors, vascular abnormalities, and up-regulated innate immune responses observed in the fracture hindlimb. We postulate that the residual pain behaviors observed in the Tac1(−/−) and RAMP1(−/−) fracture mice are attributable to the increased IL-6 levels observed in the hindpaw skin after fracture

Large Meteoroid Impact on the Moon 17 March 2013

NASA's routine monitoring of lunar impact flashes has recorded nearly 300 impacts since 2006. On 17 March 2013 the brightest event to date was observed in two 0.35m telescopes at the Marshall Space Flight Center. With a peak red magnitude brighter than 4.3 and an impact flash visible for over 1 second, the impact kinetic energy was equivalent to nearly 5 tons of TNT. A possible association with a meteor shower observed in the Earth's atmosphere will be described. Corresponding crater dimensions and observability of the impact crater by Lunar Reconnaissance Orbiter will also be discussed

A driving simulator study to explore the effects of text size on the visual demand of in-vehicle displays

Modern vehicles increasingly utilise a large display within the centre console, often with touchscreen capability, to enable access to a wide range of driving and non-driving-related functionality. The text provided on such displays can vary considerably in size, yet little is known about the effects of different text dimensions on how drivers visually sample the interface while driving and the potential implications for driving performance and user acceptance. A study is described in which sixteen people drove motorway routes in a medium-fidelity simulator and were asked to read text of varying sizes (9 mm, 8 mm, 6.5 mm, 5 mm, or 4 mm) from a central in-vehicle display. Pseudo-text was used as a stimulus to ensure that participants scanned the text in a consistent fashion that was unaffected by comprehension. There was no evidence of an effect of text size on the total time spent glancing at the display, but significant differences arose regarding how glances were distributed. Specifically, larger text sizes were associated with a high number of relatively short glances, whereas smaller text led to a smaller number of long glances. No differences were found in driving performance measures (speed, lateral lane position). Drivers overwhelmingly preferred the ‘compromise’ text sizes (6.5 mm and 8 mm). Results are discussed in relation to the development of large touchscreens within vehicles

Bioactive Endophytes Warrant Intensified Exploration and Conservation

A key argument in favor of conserving biodiversity is that as yet undiscovered biodiversity will yield products of great use to humans. However, the link between undiscovered biodiversity and useful products is largely conjectural. Here we provide direct evidence from bioassays of endophytes isolated from tropical plants and bioinformatic analyses that novel biology will indeed yield novel chemistry of potential value.We isolated and cultured 135 endophytic fungi and bacteria from plants collected in Peru. nrDNAs were compared to samples deposited in GenBank to ascertain the genetic novelty of cultured specimens. Ten endophytes were found to be as much as 15–30% different than any sequence in GenBank. Phylogenetic trees, using the most similar sequences in GenBank, were constructed for each endophyte to measure phylogenetic distance. Assays were also conducted on each cultured endophyte to record bioactivity, of which 65 were found to be bioactive.The novelty of our contribution is that we have combined bioinformatic analyses that document the diversity found in environmental samples with culturing and bioassays. These results highlight the hidden hyperdiversity of endophytic fungi and the urgent need to explore and conserve hidden microbial diversity. This study also showcases how undergraduate students can obtain data of great scientific significance