Heterofertilization in Grain Sorghum

Agronom

Consensus guidelines for the use and interpretation of angiogenesis assays

The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference

Comparison between methods of demarcation of probe of RFLP R2430E used in the selection of resistance of peanut to Meloidogyne arenaria

Neste trabalho foram comparados o método de hibridização com digoxigenina e o sistema de detecção por quimioluminescência com o método de marcação radioativa. Foram utilizados o cultivar suscetível Florunner e o resistente COAN. O DNA genômico foi isolado de folhas jovens e a análise de Southern blotting  foi  conduzida  utilizando  a  sonda  de  RFLP  R2430E. A  detecção  da  sonda  marcada  com digoxigenina foi tão sensível quanto àquela marcada pela radioatividadade, porém com vantagens sobre o método radioativo. Por ser um método seguro e apresentar custos reduzidos, o método de marcação baseado na digoxigenina e detecção por quimioluminescência é altamente recomendável.This  work  had  the  aim  of  comparing  the  method  of  hybridization  with  digoxigenin  and the chemiluminescent detection  system with  the method of  radioactive  labeled. The cultivars used were susceptible Florunner  and  resistant COAN. The genomic DNA was  isolated  from young  leaves  and Southern blotting analysis was conducted using RFLP probe R2430E. The detection of the probe labeled with digoxigenin was as sensitive as labeled by the radioactivity, however with advantages above the method  radioactive. Due  to be a  safe method and  to present  reduced costs,  the demarcation method based on the digoxigenin and chemiluminescent detection is highly recommendable