Effects on Plant Growth and Reproduction of a Peach R2R3-MYB Transcription Factor Overexpressed in Tobacco

In plants, anthocyanin production is controlled by MYB and bHLH transcription factors. In peach, among the members of these families, MYB10.1 and bHLH3 have been shown to be the most important genes for production of these pigments during fruit ripening. Anthocyanins are valuable molecules, and the overexpression of regulatory genes in annual fast-growing plants has been explored for their biotechnological production. The overexpression of peach MYB10.1 in tobacco plants induced anthocyanin pigmentation, which was particularly strong in the reproductive parts. Pigment production was the result of an up-regulation of the expression level of key genes of the flavonoid biosynthetic pathway, such as NtCHS, NtCHI, NtF3H, NtDFR, NtANS, and NtUFGT, as well as of the proanthocyanidin biosynthetic pathway such as NtLAR. Nevertheless, phenotypic alterations in transgenic tobacco lines were not only limited to anthocyanin production. Lines showing a strong phenotype (type I) exhibited irregular leaf shape and size and reduced plant height. Moreover, flowers had reduced length of anther\u2019s filament, nondehiscent anthers, reduced pistil length, aborted nectary glands, and impaired capsule development, but the reproductive parts including androecium, gynoecium, and petals were more pigmented that in wild type. Surprisingly, overexpression of peach MYB10.1 led to suppression of NtMYB305, which is required for floral development and, of one of its target genes, NECTARIN1 (NtNCE1), involved in the nectary gland formation. MYB10.1 overexpression up-regulated JA biosynthetic (NtAOS) and signaling (NtJAZd) genes, as well as 1-aminocyclopropane-1-carboxylate oxidase (NtACO) in flowers. The alteration of these hormonal pathways might be among the causes of the observed floral abnormalities with defects in both male and female gametophyte development. In particular, approximately only 30% of pollen grains of type I lines were viable, while during megaspore formation, there was a block during FG1 (St3-II). This block seemed to be associated to an excessive accumulation of callose. It can be concluded that the overexpression of peach MYB10.1 in tobacco not only regulates flavonoid biosynthesis (anthocyanin and proanthocyanidin) in the reproductive parts but also plays a role in other processes such as vegetative and reproductive development

Selenium biofortification in the 21st century: status and challenges for healthy human nutrition

Background: Selenium (Se) is an essential element for mammals and its deficiency in the diet is a global problem. Plants accumulate Se and thus represent a major source of Se to consumers. Agronomic biofortification intends to enrich crops with Se in order to secure its adequate supply by people. Scope: The goal of this review is to report the present knowledge of the distribution and processes of Se in soil and at the plant-soil interface, and of Se behaviour inside the plant in terms of biofortification. It aims to unravel the Se metabolic pathways that affect the nutritional value of edible plant products, various Se biofortification strategies in challenging environments, as well as the impact of Se-enriched food on human health. Conclusions: Agronomic biofortification and breeding are prevalent strategies for battling Se deficiency. Future research addresses nanosized Se biofortification, crop enrichment with multiple micronutrients, microbial-integrated agronomic biofortification, and optimization of Se biofortification in adverse conditions. Biofortified food of superior nutritional quality may be created, enriched with healthy Se-compounds, as well as several other valuable phytochemicals. Whether such a food source might be used as nutritional intervention for recently emerged coronavirus infections is a relevant question that deserves investigation

Anticorrosion Protection by Amine-Ionic Liquid Mixtures: Experiments and Simulations

The mixtures of aqueous amines and ionic liquids (ILs) are considered as potential solvents for CO2 capture. We report corrosion and CO2 absorption behavior of the mixed IL-amine solutions. The absorption tests were performed at 318.15 K under 0.1-2.7 MPa. The corrosion tests were carried out at 318.15 K under 2.7 MPa. Addition of [bmim][BF4] in aqueous alkanolamine solutions reduces corrosion rate for MEA by up to 72%. The CO, absorption capacity in the mixtures falls between those of aqueous MDEA and pure IL. These results allow to choose the working pressure range as a function of other parameters, such as gas pressure and mixture viscosity. According to the simulations, [bmim][BF4] participates in the gas capture through H-bonding, although the number of amine molecules is enough to capture all supplied CO, molecules. The equilibrium of the chemisorption reaction is, therefore, modified upon the stepwise IL addition. An ideal IL content for preventing corrosion is 10% w/w.CNPqHewlett-Packard Brasil LtdaCAPESPontif Catholic Univ Rio Grande Sul PUCRS, Postgrad Program Mat Engn & Technol, Ave Ipiranga 6681, BR-90619900 Porto Alegre, RS, BrazilPontif Catholic Univ Rio Grande Sul PUCRS, Sch Chem, Ave Ipiranga 6681, BR-90619900 Porto Alegre, RS, BrazilPontif Catholic Univ Rio Grande Sul PUCRS, Sch Engn, Ave Ipiranga 6681, BR-90619900 Porto Alegre, RS, BrazilUniv Fed Sao Paulo, BR-04021001 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, BR-04021001 Sao Paulo, SP, BrazilWeb of Scienc

The VANDELS survey: Dust attenuation in star-forming galaxies at z=3−4\mathbf{z=3-4}

We present the results of a new study of dust attenuation at redshifts $3 < z < 4$ based on a sample of $236$ star-forming galaxies from the VANDELS spectroscopic survey. Motivated by results from the First Billion Years (FiBY) simulation project, we argue that the intrinsic spectral energy distributions (SEDs) of star-forming galaxies at these redshifts have a self-similar shape across the mass range $8.2 \leq$ log$(M_{\star}/M_{\odot}) \leq 10.6$ probed by our sample. Using FiBY data, we construct a set of intrinsic SED templates which incorporate both detailed star formation and chemical abundance histories, and a variety of stellar population synthesis (SPS) model assumptions. With this set of intrinsic SEDs, we present a novel approach for directly recovering the shape and normalization of the dust attenuation curve. We find, across all of the intrinsic templates considered, that the average attenuation curve for star-forming galaxies at $z\simeq3.5$ is similar in shape to the commonly-adopted Calzetti starburst law, with an average total-to-selective attenuation ratio of $R_{V}=4.18\pm0.29$. We show that the optical attenuation ($A_V$) versus stellar mass ($M_{\star}$) relation predicted using our method is consistent with recent ALMA observations of galaxies at $2<z<3$ in the \emph{Hubble} \emph{Ultra} \emph{Deep} \emph{Field} (HUDF), as well as empirical $A_V - M_{\star}$ relations predicted by a Calzetti-like law. Our results, combined with other literature data, suggest that the $A_V - M_{\star}$ relation does not evolve over the redshift range $0<z<5$, at least for galaxies with log$(M_{\star}/M_{\odot}) \gtrsim 9.5$. Finally, we present tentative evidence which suggests that the attenuation curve may become steeper at log$(M_{\star}/M_{\odot}) \lesssim 9.0$.Comment: 16 pages, 12 figures, accepted for publication in MNRA

The VANDELS survey: Dust attenuation in star-forming galaxies at z=3−4\mathbf{z=3-4}

We present the results of a new study of dust attenuation at redshifts $3 < z < 4$ based on a sample of $236$ star-forming galaxies from the VANDELS spectroscopic survey. Motivated by results from the First Billion Years (FiBY) simulation project, we argue that the intrinsic spectral energy distributions (SEDs) of star-forming galaxies at these redshifts have a self-similar shape across the mass range $8.2 \leq$ log$(M_{\star}/M_{\odot}) \leq 10.6$ probed by our sample. Using FiBY data, we construct a set of intrinsic SED templates which incorporate both detailed star formation and chemical abundance histories, and a variety of stellar population synthesis (SPS) model assumptions. With this set of intrinsic SEDs, we present a novel approach for directly recovering the shape and normalization of the dust attenuation curve. We find, across all of the intrinsic templates considered, that the average attenuation curve for star-forming galaxies at $z\simeq3.5$ is similar in shape to the commonly-adopted Calzetti starburst law, with an average total-to-selective attenuation ratio of $R_{V}=4.18\pm0.29$. We show that the optical attenuation ($A_V$) versus stellar mass ($M_{\star}$) relation predicted using our method is consistent with recent ALMA observations of galaxies at $2<z<3$ in the \emph{Hubble} \emph{Ultra} \emph{Deep} \emph{Field} (HUDF), as well as empirical $A_V - M_{\star}$ relations predicted by a Calzetti-like law. Our results, combined with other literature data, suggest that the $A_V - M_{\star}$ relation does not evolve over the redshift range $0<z<5$, at least for galaxies with log$(M_{\star}/M_{\odot}) \gtrsim 9.5$. Finally, we present tentative evidence which suggests that the attenuation curve may become steeper at log$(M_{\star}/M_{\odot}) \lesssim 9.0$.Comment: 16 pages, 12 figures, accepted for publication in MNRA

FATZ, a filamin-, actinin-, and telethonin-binding protein of the Z-disc of skeletal muscle

We report the identification and characterization of a novel 32-kDa protein expressed in skeletal muscle and located in the Z-disc of the sarcomere. We found that this protein binds to three other Z-disc proteins; therefore, we have-named it FATZ, gamma -filamin/ABP-L, alpha -actinin and telethonin binding protein of the Z-disc. From yeast two-hybrid experiments we are able to show that the SR3-SR4 domains of alpha -actinin 2 are required to bind the COOH-terminal region of the FATZ as does gamma -filamin/ABP-L, Furthermore, by using a glutathione S-transferase overlay assay we find that FATZ also binds telethonin. The level of FATZ protein in muscle cells increases during differentiation, being clearly detectable before the onset of myosin, Although FATZ has no known interaction domains, it would appear to be involved in a complex network of interactions with other Z-band components. On the basis of the information known about its binding partners, we could envisage a central role for FATZ in the: myofibrillogenesis, After screening our muscle expressed sequence tag data base and the public expressed sequence tag data bases, we were able to assemble two other muscle transcripts that show a high level of identity with FATZ in two different domains. Therefore, FATZ may be the first member of a small family of novel muscle proteins

Viscoelastic gels of guar and xanthan gum mixtures provide long-term stabilization of iron micro- and nanoparticles

Iron micro- and nanoparticles used for groundwater remediation and medical applications are prone to fast aggregation and sedimentation. Diluted single biopolymer water solutions of guar gum (GG) or xanthan gum (XG) can stabilize these particles for few hours providing steric repulsion and by increasing the viscosity of the suspension. The goal of the study is to demonstrate that amending GG solutions with small amounts of XG (XG/GG weight ratio 1:19; 3 g/L of total biopolymer concentration) can significantly improve the capability of the biopolymer to stabilize highly concentrated iron micro- and nanoparticle suspensions. The synergistic effect between GG and XG generates a viscoelastic gel that can maintain 20 g/L iron particles suspended for over 24 h. This is attributed to (i) an increase in the static viscosity, (ii) a combined polymer structure the yield stress of which contrasts the downward stress exerted by the iron particles, and (iii) the adsorption of the polymers to the iron surface having an anchoring effect on the particles. The XG/GG viscoelastic gel is characterized by a marked shear thinning behavior. This property, coupled with the low biopolymer concentration, determines small viscosity values at high shear rates, facilitating the injection in porous media. Furthermore, the thermosensitivity of the soft elastic polymeric network promotes higher stability and longer storage times at low temperatures and rapid decrease of viscosity at higher temperatures. This feature can be exploited in order to improve the flowability and the delivery of the suspensions to the target as well as to effectively tune and control the release of the iron particle

Renin Angiotensin System Blockers and Risk of Mortality in Hypertensive Patients Hospitalized for COVID-19: An Italian Registry

Background: It is uncertain whether exposure to renin\u2013angiotensin system (RAS) modifiers affects the severity of the new coronavirus disease 2019 (COVID-19) because most of the available studies are retrospective. Methods: We tested the prognostic value of exposure to RAS modifiers (either angiotensin-converting enzyme inhibitors [ACE-Is] or angiotensin receptor blockers [ARBs]) in a prospective study of hypertensive patients with COVID-19. We analyzed data from 566 patients (mean age 75 years, 54% males, 162 ACE-Is users, and 147 ARBs users) hospitalized in five Italian hospitals. The study used systematic prospective data collection according to a pre-specified protocol. All-cause mortality during hospitalization was the primary outcome. Results: Sixty-six patients died during hospitalization. Exposure to RAS modifiers was associated with a significant reduction in the risk of in-hospital mortality when compared to other BP-lowering strategies (odds ratio [OR]: 0.54, 95% confidence interval [CI]: 0.32 to 0.90, p = 0.019). Exposure to ACE-Is was not significantly associated with a reduced risk of in-hospital mortality when compared with patients not treated with RAS modifiers (OR: 0.66, 95% CI: 0.36 to 1.20, p = 0.172). Conversely, ARBs users showed a 59% lower risk of death (OR: 0.41, 95% CI: 0.20 to 0.84, p = 0.016) even after allowance for several prognostic markers, including age, oxygen saturation, occurrence of severe hypotension during hospitalization, and lymphocyte count (adjusted OR: 0.37, 95% CI: 0.17 to 0.80, p = 0.012). The discontinuation of RAS modifiers during hospitalization did not exert a significant effect (p = 0.515). Conclusions: This prospective study indicates that exposure to ARBs reduces mortality in hospitalized patients with COVID-19

Feedback and the Structure of Simulated Galaxies at redshift z=2

We study the properties of simulated high-redshift galaxies using cosmological N-body/gasdynamical runs from the OverWhelmingly Large Simulations (OWLS) project. The runs contrast several feedback implementations of varying effectiveness: from no-feedback, to supernova-driven winds to powerful AGN-driven outflows. These different feedback models result in large variations in the abundance and structural properties of bright galaxies at z=2. We find that feedback affects the baryonic mass of a galaxy much more severely than its spin, which is on average roughly half that of its surrounding dark matter halo in our runs. Feedback induces strong correlations between angular momentum content and galaxy mass that leave their imprint on galaxy scaling relations and morphologies. Encouragingly, we find that galaxy disks are common in moderate-feedback runs, making up typically ~50% of all galaxies at the centers of haloes with virial mass exceeding 1e11 M_sun. The size, stellar masses, and circular speeds of simulated galaxies formed in such runs have properties that straddle those of large star-forming disks and of compact early-type galaxies at z=2. Once the detailed abundance and structural properties of these rare objects are well established it may be possible to use them to gauge the overall efficacy of feedback in the formation of high redshift galaxies.Comment: 16 pages, 12 figures. Accepted for publication in MNRAS. Minor changes to match published versio