462 research outputs found
“They Don’t Think Like Us”: Exploring Attitudes of Non-Transgender Students Toward Transgender People Using Discourse Analysis
Solutions for 10,000 Eclipsing Binaries in the Bulge Fields of OGLE II Using DEBiL
We have developed a fully-automated pipeline for systematically identifying
and analyzing eclipsing binaries within large datasets of light curves. The
pipeline is made up of multiple tiers which subject the light curves to
increasing levels of scrutiny. After each tier, light curves that did not
conform to a given criteria were filtered out of the pipeline, reducing the
load on the following, more computationally intensive tiers. As a central
component of the pipeline, we created the fully automated Detached Eclipsing
Binary Light curve fitter (DEBiL), which rapidly fits large numbers of light
curves to a simple model. Using the results of DEBiL, light curves of interest
can be flagged for follow-up analysis. As a test case, we analyzed the 218699
light curves within the bulge fields of the OGLE II survey and produced 10862
model fits. We point out a small number of extreme examples as well as
unexpected structure found in several of the population distributions. We
expect this approach to become increasingly important as light curve datasets
continue growing in both size and number.Comment: Accepted for publication in ApJ, 36 pages, 15 figures, 5 tables. See
http://cfa-www.harvard.edu/~jdevor/DEBiL.html for high-resolution figures and
further informatio
A Method For Eclipsing Component Identification In Large Photometric Datasets
We describe an automated method for assigning the most likely physical
parameters to the components of an eclipsing binary (EB), using only its
photometric light curve and combined color. In traditional methods (e.g. WD and
EBOP) one attempts to optimize a multi-parameter model over many iterations, so
as to minimize the chi-squared value. We suggest an alternative method, where
one selects pairs of coeval stars from a set of theoretical stellar models, and
compares their simulated light curves and combined colors with the
observations. This approach greatly reduces the EB parameter-space over which
one needs to search, and allows one to determine the components' masses, radii
and absolute magnitudes, without spectroscopic data. We have implemented this
method in an automated program using published theoretical isochrones and
limb-darkening coefficients. Since it is easy to automate, this method lends
itself to systematic analyses of datasets consisting of photometric time series
of large numbers of stars, such as those produced by OGLE, MACHO, TrES, HAT,
and many others surveys.Comment: 6 pages, 5 figures. To appear in the conference proceedings of "Close
Binaries in the 21st Century: New Opportunities and Challenges", Syros,
Greece, 27-30 June, 200
Searching for transits in the Wide Field Camera Transit Survey with difference-imaging light curves
The Wide Field Camera Transit Survey is a pioneer program aiming at for searching extra-solar planets in the near-infrared. The images from the survey are processed by a data reduction pipeline, which uses aperture photometry to construct the light curves. We produce an alternative set of light curves using the difference-imaging method for the most complete field in the survey and carry out a quantitative comparison between the photometric precision achieved with both methods. The results show that differencephotometry light curves present an important improvement for stars with J > 16. We report an implementation on the box-fitting transit detection algorithm, which performs a trapezoid-fit to the folded light curve, providing more accurate results than the boxfitting model. We describe and optimize a set of selection criteria to search for transit candidates, including the V-shape parameter calculated by our detection algorithm. The optimized selection criteria are applied to the aperture photometry and difference-imaging light curves, resulting in the automatic detection of the best 200 transit candidates from a sample of ~475 000 sources. We carry out a detailed analysis in the 18 best detections and classify them as transiting planet and eclipsing binary candidates. We present one planet candidate orbiting a late G-type star. No planet candidate around M-stars has been found, confirming the null detection hypothesis and upper limits on the occurrence rate of short-period giant planets around M-dwarfs presented in a prior study. We extend the search for transiting planets to stars with J ≤ 18, which enables us to set a stricter upper limit of 1.1%. Furthermore, we present the detection of five faint extremely-short period eclipsing binaries and three M-dwarf/M-dwarf binary candidates. The detections demonstrate the benefits of using the difference-imaging light curves, especially when going to fainter magnitudes.Peer reviewe
A Small Conductance Calcium-Activated K<sup>+</sup> Channel in C. elegans, KCNL-2, Plays a Role in the Regulation of the Rate of Egg-Laying
In the nervous system of mice, small conductance calcium-activated potassium (SK) channels function to regulate neuronal excitability through the generation of a component of the medium afterhyperpolarization that follows action potentials. In humans, irregular action potential firing frequency underlies diseases such as ataxia, epilepsy, schizophrenia and Parkinson's disease. Due to the complexity of studying protein function in the mammalian nervous system, we sought to characterize an SK channel homologue, KCNL-2, in C. elegans, a genetically tractable system in which the lineage of individual neurons was mapped from their early developmental stages. Sequence analysis of the KCNL-2 protein reveals that the six transmembrane domains, the potassium-selective pore and the calmodulin binding domain are highly conserved with the mammalian homologues. We used widefield and confocal fluorescent imaging to show that a fusion construct of KCNL-2 with GFP in transgenic lines is expressed in the nervous system of C. elegans. We also show that a KCNL-2 null strain, kcnl-2(tm1885), demonstrates a mild egg-laying defective phenotype, a phenotype that is rescued in a KCNL-2-dependent manner. Conversely, we show that transgenic lines that overexpress KCNL-2 demonstrate a hyperactive egg-laying phenotype. In this study, we show that the vulva of transgenic hermaphrodites is highly innervated by neuronal processes and by the VC4 and VC5 neurons that express GFP-tagged KCNL-2. We propose that KCNL-2 functions in the nervous system of C. elegans to regulate the rate of egg-laying. © 2013 Chotoo et al
Four ultra-short period eclipsing M-dwarf binaries in the WFCAM Transit Survey
We report on the discovery of four ultra-short period (P<0.18 days) eclipsing
M-dwarf binaries in the WFCAM Transit Survey. Their orbital periods are
significantly shorter than of any other known main-sequence binary system, and
are all significantly below the sharp period cut-off at P~0.22 days as seen in
binaries of earlier type stars. The shortest-period binary consists of two M4
type stars in a P=0.112 day orbit. The binaries are discovered as part of an
extensive search for short-period eclipsing systems in over 260,000 stellar
lightcurves, including over 10,000 M-dwarfs down to J=18 mag, yielding 25
binaries with P<0.23 days. In a popular paradigm, the evolution of short period
binaries of cool main-sequence stars is driven by loss of angular momentum
through magnetised winds. In this scheme, the observed P~0.22 day period
cut-off is explained as being due to timescales that are too long for
lower-mass binaries to decay into tighter orbits. Our discovery of low-mass
binaries with significantly shorter orbits implies that either these timescales
have been overestimated for M-dwarfs, e.g. due to a higher effective magnetic
activity, or that the mechanism for forming these tight M-dwarf binaries is
different from that of earlier type main-sequence stars.Comment: 22 pages, 17 figures, 3 tables Accepted for publication in MNRA
The K+ Channel Opener 1-EBIO Potentiates Residual Function of Mutant CFTR in Rectal Biopsies from Cystic Fibrosis Patients
BACKGROUND: The identification of strategies to improve mutant CFTR function remains a key priority in the development of new treatments for cystic fibrosis (CF). Previous studies demonstrated that the K⁺ channel opener 1-ethyl-2-benzimidazolone (1-EBIO) potentiates CFTR-mediated Cl⁻ secretion in cultured cells and mouse colon. However, the effects of 1-EBIO on wild-type and mutant CFTR function in native human colonic tissues remain unknown. METHODS: We studied the effects of 1-EBIO on CFTR-mediated Cl⁻ secretion in rectal biopsies from 47 CF patients carrying a wide spectrum of CFTR mutations and 57 age-matched controls. Rectal tissues were mounted in perfused micro-Ussing chambers and the effects of 1-EBIO were compared in control tissues, CF tissues expressing residual CFTR function and CF tissues with no detectable Cl⁻ secretion. RESULTS: Studies in control tissues demonstrate that 1-EBIO activated CFTR-mediated Cl⁻ secretion in the absence of cAMP-mediated stimulation and potentiated cAMP-induced Cl⁻ secretion by 39.2±6.7% (P<0.001) via activation of basolateral Ca²⁺-activated and clotrimazole-sensitive KCNN4 K⁺ channels. In CF specimens, 1-EBIO potentiated cAMP-induced Cl⁻ secretion in tissues with residual CFTR function by 44.4±11.5% (P<0.001), but had no effect on tissues lacking CFTR-mediated Cl⁻ conductance. CONCLUSIONS: We conclude that 1-EBIO potentiates Cl⁻secretion in native CF tissues expressing CFTR mutants with residual Cl⁻ channel function by activation of basolateral KCNN4 K⁺ channels that increase the driving force for luminal Cl⁻ exit. This mechanism may augment effects of CFTR correctors and potentiators that increase the number and/or activity of mutant CFTR channels at the cell surface and suggests KCNN4 as a therapeutic target for CF
Imaging of Functional Connectivity in the Mouse Brain
Functional neuroimaging (e.g., with fMRI) has been difficult to perform in mice, making it challenging to translate between human fMRI studies and molecular and genetic mechanisms. A method to easily perform large-scale functional neuroimaging in mice would enable the discovery of functional correlates of genetic manipulations and bridge with mouse models of disease. To satisfy this need, we combined resting-state functional connectivity mapping with optical intrinsic signal imaging (fcOIS). We demonstrate functional connectivity in mice through highly detailed fcOIS mapping of resting-state networks across most of the cerebral cortex. Synthesis of multiple network connectivity patterns through iterative parcellation and clustering provides a comprehensive map of the functional neuroarchitecture and demonstrates identification of the major functional regions of the mouse cerebral cortex. The method relies on simple and relatively inexpensive camera-based equipment, does not require exogenous contrast agents and involves only reflection of the scalp (the skull remains intact) making it minimally invasive. In principle, fcOIS allows new paradigms linking human neuroscience with the power of molecular/genetic manipulations in mouse models
Global and regional brain metabolic scaling and its functional consequences
Background: Information processing in the brain requires large amounts of
metabolic energy, the spatial distribution of which is highly heterogeneous
reflecting complex activity patterns in the mammalian brain.
Results: Here, it is found based on empirical data that, despite this
heterogeneity, the volume-specific cerebral glucose metabolic rate of many
different brain structures scales with brain volume with almost the same
exponent around -0.15. The exception is white matter, the metabolism of which
seems to scale with a standard specific exponent -1/4. The scaling exponents
for the total oxygen and glucose consumptions in the brain in relation to its
volume are identical and equal to , which is significantly larger
than the exponents 3/4 and 2/3 suggested for whole body basal metabolism on
body mass.
Conclusions: These findings show explicitly that in mammals (i)
volume-specific scaling exponents of the cerebral energy expenditure in
different brain parts are approximately constant (except brain stem
structures), and (ii) the total cerebral metabolic exponent against brain
volume is greater than the much-cited Kleiber's 3/4 exponent. The
neurophysiological factors that might account for the regional uniformity of
the exponents and for the excessive scaling of the total brain metabolism are
discussed, along with the relationship between brain metabolic scaling and
computation.Comment: Brain metabolism scales with its mass well above 3/4 exponen
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