Essential and checkpoint functions of budding yeast ATM and ATR during meiotic prophase are facilitated by differential phosphorylation of a meiotic adaptor protein, Hop1

A hallmark of the conserved ATM/ATR signalling is its ability to mediate a wide range of functions utilizing only a limited number of adaptors and effector kinases. During meiosis, Tel1 and Mec1, the budding yeast ATM and ATR, respectively, rely on a meiotic adaptor protein Hop1, a 53BP1/Rad9 functional analog, and its associated kinase Mek1, a CHK2/Rad53-paralog, to mediate multiple functions: control of the formation and repair of programmed meiotic DNA double strand breaks, enforcement of inter-homolog bias, regulation of meiotic progression, and implementation of checkpoint responses. Here, we present evidence that the multi-functionality of the Tel1/Mec1-to-Hop1/Mek1 signalling depends on stepwise activation of Mek1 that is mediated by Tel1/Mec1 phosphorylation of two specific residues within Hop1: phosphorylation at the threonine 318 (T318) ensures the transient basal level Mek1 activation required for viable spore formation during unperturbed meiosis. Phosphorylation at the serine 298 (S298) promotes stable Hop1-Mek1 interaction on chromosomes following the initial phospho-T318 mediated Mek1 recruitment. In the absence of Dmc1, the phospho-S298 also promotes Mek1 hyper-activation necessary for implementing meiotic checkpoint arrest. Taking these observations together, we propose that the Hop1 phospho-T318 and phospho-S298 constitute key components of the Tel1/Mec1- based meiotic recombination surveillance (MRS) network and facilitate effective coupling of meiotic recombination and progression during both unperturbed and challenged meiosis

Molecular evolution of HoxA13 and the multiple origins of limbless morphologies in amphibians and reptiles

Developmental processes and their results, morphological characters, are inherited through transmission of genes regulating development. While there is ample evidence that cis-regulatory elements tend to be modular, with sequence segments dedicated to different roles, the situation for proteins is less clear, being particularly complex for transcription factors with multiple functions. Some motifs mediating protein-protein interactions may be exclusive to particular developmental roles, but it is also possible that motifs are mostly shared among different processes. Here we focus on HoxA13, a protein essential for limb development. We asked whether the HoxA13 amino acid sequence evolved similarly in three limbless clades: Gymnophiona, Amphisbaenia and Serpentes. We explored variation in ω (dN/dS) using a maximum-likelihood framework and HoxA13sequences from 47 species. Comparisons of evolutionary models provided low ω global values and no evidence that HoxA13 experienced relaxed selection in limbless clades. Branch-site models failed to detect evidence for positive selection acting on any site along branches of Amphisbaena and Gymnophiona, while three sites were identified in Serpentes. Examination of alignments did not reveal consistent sequence differences between limbed and limbless species. We conclude that HoxA13 has no modules exclusive to limb development, which may be explained by its involvement in multiple developmental processes

Watermelon production on stored rainwater in Sahelian sandy soils

Watermelon [Citrullus lanatus (Thumb) Matsun and Nakai ] is an important cash crop in West Africa where it is cultivated under rainfed conditions. The objective of this work was to identify best cultural practices for production of watermelons in the Sahel on stored rainwater in acid sandy soils. The experiments were carried out at the ICRISAT Sadore research center in Niger during two consecutive dry seasons, 2003 - 2004 and 2004 - 2005. Three soil management treatments were applied: microcatchments (also called half-moons), planting pits (also called zaï) and sowing on flat land. Each of these three treatments came with and without a soil amendment comprising of 500 g of manure mixed with 24 g of a complete (NPK) fertilizer (15-15-15) individually applied to each planting hill. Two watermelon cultivars were tested: ‘Malali’ and ‘Kaolack’. In each of the two years the experiments were sown on the 1st and on the 21st of September. Fruit and biomass yield, fruit Total Soluble Solids (TSS), days to fruiting and harvesting were determined. Soil fertility, root development and other physiological parameters were monitored to explain some of the differences between treatments. Soil amendments increased marketable yields from 1.3 to 3.5 tons ha-1 on average. Marketable yields at the first planting date were double the yields of the second planting date (3.2 vs. 1.6 tons ha-1). Yield differences were due to changes in fruit number not in fruit weight. Deep placement of soil amendments resulted in significant root development in deeper soil layers. Highest watermelon yields were achieved when sowing the Malali cultivar in amended planting pits on September 1st giving a yield of 8.2 tons ha-1

Plasmodium falciparum metacaspase PfMCA-1 triggers a z-VAD-fmk inhibitable protease to promote cell death.

Activation of proteolytic cell death pathways may circumvent drug resistance in deadly protozoan parasites such as Plasmodium falciparum and Leishmania. To this end, it is important to define the cell death pathway(s) in parasites and thus characterize proteases such as metacaspases (MCA), which have been reported to induce cell death in plants and Leishmania parasites. We, therefore, investigated whether the cell death function of MCA is conserved in different protozoan parasite species such as Plasmodium falciparum and Leishmania major, focusing on the substrate specificity and functional role in cell survival as compared to Saccharomyces cerevisae. Our results show that, similarly to Leishmania, Plasmodium MCA exhibits a calcium-dependent, arginine-specific protease activity and its expression in yeast induced growth inhibition as well as an 82% increase in cell death under oxidative stress, a situation encountered by parasites during the host or when exposed to drugs such as artemisins. Furthermore, we show that MCA cell death pathways in both Plasmodium and Leishmania, involve a z-VAD-fmk inhibitable protease. Our data provide evidence that MCA from both Leishmania and Plasmodium falciparum is able to induce cell death in stress conditions, where it specifically activates a downstream enzyme as part of a cell death pathway. This enzymatic activity is also induced by the antimalarial drug chloroquine in erythrocytic stages of Plasmodium falciparum. Interestingly, we found that blocking parasite cell death influences their drug sensitivity, a result which could be used to create therapeutic strategies that by-pass drug resistance mechanisms by acting directly on the innate pathways of protozoan cell death

The economics of low pressure drip irrigation and hand watering for vegetable production in the Sahel

Low pressure drip irrigation is being promoted in Sub Saharan Africa as an alternative to traditional methods of small scale irrigation of vegetables. The African Market Garden (AMG) is a horticultural production system for smallholders based on low-pressure drip irrigation combined with an improved crop management package. The agronomic and economic performance of the AMG is compared to two gardens irrigated manually with watering cans. One of these gardens is managed according to the same improved crop management package as in the AMG, this treatment is called Improved Management (IM). The other garden is managed according to common practices of vegetable producers in the area, this treatment is called the Farmer Practice (FP). Crop productivity, labor and water use were monitored for two vegetable species (okra and eggplants). The experiment was performed on-station in Niger on three adjacent 500 m2 plots in a sandy acid soil. It was found that improved crop management practices greatly enhance crop productivity over traditional methods at comparable production costs. The AMG gave higher crop yields and higher returns to investment than the treatments irrigated with watering cans. Labor accounts for up to 45% of the production cost in vegetable gardens irrigated by hand, where 80% of the producer time is spent on irrigation. The total labor requirement for the drip irrigated AMG was on average 1.1 man hours per day against 4.7 man hours per day for the Farmers Practice on a 500 m2 garden. Returns on labor are at least double for the AMG against the other treatments. The returns on land from eggplant were found to be US$1.7, 0.8 and 0.1 per m2 for the AMG, IM and FP respectively. The returns on water for the cultivation of eggplant are around US$ 2 per m3 in the AMG, against US$ 0.1 in the Farmers Practice. This experiment showed the strong positive impact of drip irrigation and improved crop management practices on profits at minimal environmental costs, indicating that transformation of existing practices poses a considerable potential towards sustainable agricultural developmen

The Dryland Eco-Farm: A Potential Solution to the Main Constraints of Rain-Fed Agriculture in the Semi-Arid Tropics of Africa

This chapter presents the results of studies on a production system called Dryland Eco-Farm (DEF) that addresses a range of constraints to agricultural productivity in dryland Africa. It combines the use of live hedges and alleys of Acacia colei, “demi-lunes” in which are planted domesticated Ziziphus mauritiania. Annual crops like pearl millet (Pennisetum glaucum (L.) R.Br.), cowpeas and roselle (Hibiscus sabdariffa) are planted in rotation. This trial tests the effect of the system on (1) soil erosion control, soil fertility and water use efficiency, (2) crop yield and biomass production, and (3) improving income generation and diversification. Average pearl millet yields in the DEF were twice the control (880 vs. 430 kg ha–1) when no mineral fertilizer was applied. With the application of NPK, millet yields were almost similar under both conditions (950 vs. 780 kg ha–1). Cowpea yields were on average seven times higher than the control without NPK (1,400 vs. 200 kg ha–1 total biomass) and three times with NPK (1,850 vs. 650 kg ha–1 total biomass). Roselle yield increased four times on average without NPK (205 vs. 60 kg ha–1 calices yield) and two times with NPK (234 vs. 114 kg ha–1). Therefore, the system has the potential to produce yield response similar to that of the recommended rate of 100 kg of the 15-15-15 fertilizer per ha. The return to land is estimated at US 224 for the DEF compared to US224fortheDEFcomparedtoUS77 for the traditional millet–cowpea system. This system has the potential to improve productivity and rural livelihood in the drylands of Africa while sustaining the natural resources base

Mouse HORMAD1 and HORMAD2, two conserved meiotic chromosomal proteins, are depleted from synapsed chromosome axes with the help of TRIP13 AAA-ATPase

Meiotic crossovers are produced when programmed double-strand breaks (DSBs) are repaired by recombination from homologous chromosomes (homologues). In a wide variety of organisms, meiotic HORMA-domain proteins are required to direct DSB repair towards homologues. This inter-homologue bias is required for efficient homology search, homologue alignment, and crossover formation. HORMA-domain proteins are also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs. We examined the behavior of two previously uncharacterized meiosis-specific mouse HORMA-domain proteins-HORMAD1 and HORMAD2-in wild-type mice and in mutants defective in DSB processing or SC formation. HORMADs are preferentially associated with unsynapsed chromosome axes throughout meiotic prophase. We observe a strong negative correlation between SC formation and presence of HORMADs on axes, and a positive correlation between the presumptive sites of high checkpoint-kinase ATR activity and hyper-accumulation of HORMADs on axes. HORMADs are not depleted from chromosomes in mutants that lack SCs. In contrast, DSB formation and DSB repair are not absolutely required for depletion of HORMADs from synapsed axes. A simple interpretation of these findings is that SC formation directly or indirectly promotes depletion of HORMADs from chromosome axes. We also find that TRIP13 protein is required for reciprocal distribution of HORMADs and the SYCP1/SC-component along chromosome axes. Similarities in mouse and budding yeast meiosis suggest that TRIP13/Pch2 proteins have a conserved role in establishing mutually exclusive HORMAD-rich and synapsed chromatin domains in both mouse and yeast. Taken together, our observations raise the possibility that involvement of meiotic HORMA-domain proteins in the regulation of homologue interactions is conserved in mammals

Considerations of Efficiency and Distributive Justice in Multidimensional Poverty Measurement

Ab den 1980er Jahren entwickelte Amartya Sen eine neue Wohlfahrtstheorie: den Capability Approach (Sen, 1979; 1985; 1992; 1999; 2009). Dabei ersetzen Capabilities und Functionings, d.h. das, was Personen tatsächlich in der Lage sind zu tun und zu sein, den traditionellen Einkommensansatz. Armut ist im Capability Approach das Unvermögen, ein bestimmtes Minimum an zentralen Capabilities zu erreichen, die benötigt werden, um das Leben nach den eigenen Vorstellungen zu gestalten. Der Capability Approach hat so viele interessante Eigenschaften, besonders in Bezug auf die Armutsmessung, dass er zunehmend Einfluss in der Wohlfahrtsökonomie gewinnt. Diese Entwicklung wird durch empirische Untersuchungen gefördert, die zeigen, dass dieser multidimensionale Ansatz zur Armutsmessung deutlich andere Ergebnisse generiert als der traditionelle Einkommensansatz (vgl. Klasen, 2000, Alkire und Santos, 2010, Figari, 2012). Der derzeitige multidimensionale Ansatz hat jedoch eine methodische Schwäche: Ungleichheit zwischen Armutsdimensionen wird entweder als Korrelationssensitivität definiert – womit Effizienz aber nicht Verteilungsgerechtigkeit berücksichtigt wird – oder als die Verteilung multipler Mangelerscheinungen in einer Gesellschaft – womit Verteilungsgerechtigkeit aber nicht Effizienz berücksichtigt wird. Die ersten beiden Kapitel dieser Dissertation widmen sich der Behebung dieser methodischen Schwäche. Dazu wird Ungleichheit zwischen Dimensionen zunächst als „korrelationssensitive Verteilung multipler Mangelerscheinungen in einer Gesellschaft“ definiert. Die ersten beiden Kapitel operationalisieren diese erweiterte Definition für den Fall ordinaler und kardinaler Armutsindices. Im Einzelnen wird ein neues Axiom für den ordinalen sowie den kardinalen Fall eingeführt, das das Ausmaß, mit dem ein Ungleichheitsfördernder Tausch Armut sinken (oder steigen) lässt, von der Beziehung zwischen den Armutsdimensionen abhängig macht. Diese Neuerung wird benutzt um eine neue Klasse ordinaler bzw. kardinaler Armutsindices herzuleiten. Diese zwei Klassen sind die ersten additiven Armutsindices die in der Lage sind, sowohl Ungleichheit als auch Korrelationssensitivität zu erfassen. Das dritte Kapitel nutzt das deutsche sozio-ökonomische Panel um zwei ordinale Armutsindices für Deutschland vorzuschlagen, die auf der zuvor entwickelten Methode basieren: den „Deutschen Korrelationssensitiven Armutsindex“ und den „Subjektiven Korrelationssensitiven Armutsindex“. Die beiden Indices werden mit dem offiziellen deutschen Armutsmaß, der Armutsgefährdungsquote, über Dimensionen, Regionen und über die Zeit hinweg verglichen. Die Resultate zeigen vor allem eines: die signifikanten Unterschiede in der Beurteilung von Armut und Armutstrends die durch die verschiedenen Indices versursacht werden und den hohen Mehrwert den die Operationalisierung des Capability Approachs darstellt

Interaction of plant growth regulators and reactive oxygen species to regulate petal senescence in wallflowers (Erysimum linifolium)

Background In many species floral senescence is coordinated by ethylene. Endogenous levels rise, and exogenous application accelerates senescence. Furthermore, floral senescence is often associated with increased reactive oxygen species, and is delayed by exogenously applied cytokinin. However, how these processes are linked remains largely unresolved. Erysimum linifolium (wallflower) provides an excellent model for understanding these interactions due to its easily staged flowers and close taxonomic relationship to Arabidopsis. This has facilitated microarray analysis of gene expression during petal senescence and provided gene markers for following the effects of treatments on different regulatory pathways. Results In detached Erysimum linifolium (wallflower) flowers ethylene production peaks in open flowers. Furthermore senescence is delayed by treatments with the ethylene signalling inhibitor silver thiosulphate, and accelerated with ethylene released by 2-chloroethylphosphonic acid. Both treatments with exogenous cytokinin, or 6-methyl purine (which is an inhibitor of cytokinin oxidase), delay petal senescence. However, treatment with cytokinin also increases ethylene biosynthesis. Despite the similar effects on senescence, transcript abundance of gene markers is affected differentially by the treatments. A significant rise in transcript abundance of WLS73 (a putative aminocyclopropanecarboxylate oxidase) was abolished by cytokinin or 6-methyl purine treatments. In contrast, WFSAG12 transcript (a senescence marker) continued to accumulate significantly, albeit at a reduced rate. Silver thiosulphate suppressed the increase in transcript abundance both of WFSAG12 and WLS73. Activity of reactive oxygen species scavenging enzymes changed during senescence. Treatments that increased cytokinin levels, or inhibited ethylene action, reduced accumulation of hydrogen peroxide. Furthermore, although auxin levels rose with senescence, treatments that delayed early senescence did not affect transcript abundance of WPS46, an auxin-induced gene. Conclusions A model for the interaction between cytokinins, ethylene, reactive oxygen species and auxin in the regulation of floral senescence in wallflowers is proposed. The combined increase in ethylene and reduction in cytokinin triggers the initiation of senescence and these two plant growth regulators directly or indirectly result in increased reactive oxygen species levels. A fall in conjugated auxin and/or the total auxin pool eventually triggers abscission