Dinosaur tracks from the Early Cretaceous (Albian) of Parede (Cascais, Portugal): new contributions for the sauropod palaeobiology of the Iberian Peninsula

A recently discovered Early Cretaceous (early late Albian) dinosaur tracksite at Parede beach (Cascais, Portugal) reveals evidence of dinoturbation and at least two sauropod trackways. One of these trackways can be classified as narrow-gauge, which represents unique evidence in the Albian of the Iberian Peninsula and provides for the improvement of knowledge of this kind of trackway and its probable trackmaker, in an age when the sauropod record is scarce. These dinosaur tracks are preserved on the upper surface of a marly limestone bed that belongs to the Galé Formation (Água Doce Member, middle to lower upper Albian). The study of thin-sections of the beds C22/24 and C26 in the Parede section has revealed a microfacies composed of foraminifers, radiolarians, ostracods, corals, bivalves, gastropods, and echinoids in a mainly wackestone texture with biomicritic matrix. These assemblages match with the lithofacies, marine molluscs, echinids, and ichnofossils sampled from the section and indicate a shallow marine, inner shelf palaeoenvironment with a shallowing-upward trend. The biofacies and the sequence analysis are compatible with the early late Albian age attributed to the tracksite. These tracks and the moderate dinoturbation index indicate sauropod activity in this palaeoenvironment. Titanosaurs can be dismissed as possible trackmakers on the basis of the narrow-gauge trackway, and probably by the kidney-shaped manus morphology and the pes-dominated configuration of the trackway. Narrow-gauge sauropod trackways have been positively associated with coastal palaeoenvironments, and the Parede tracksite supports this interpretation. In addition, this tracksite adds new data about the presence of sauropod pes-dominated trackways in cohesive substrates. As the Portuguese Cretaceous sauropod osteological remains are very scarce, the Parede tracksite yields new and relevant evidence of these dinosaurs. Furthermore, the Parede tracksite is the youngest evidence of sauropods in the Portuguese record and some of the rare evidence of sauropods in Europe during the Albian. This discovery enhances the palaeobiological data for the Early Cretaceous Sauropoda of the Iberian Peninsula, where the osteological remains of these dinosaurs are relatively scarce in this region of southwestern Europe. Therefore, this occurrence is also of overall interest due to its impact on Cretaceous Sauropoda palaeobiogeography.El reciente descubrimiento de huellas de dinosaurios del Cretácico Inferior (inicio del Albiense tardío) en la playa de Parede (Cascais, Portugal) ha revelado evidencias de dinoturbación y al menos dos rastros de saurópodos. Uno de ellos puede clasificarse como estrecho y representa la única evidencia de este tipo de rastros en el Albiense de la Península Ibérica. Estos nuevos datos permiten mejorar el conocimiento tanto de este tipo de rastros como de su posible productor, en una edad en la que el registro de saurópodos es escaso. Las huellas de dinosaurios se localizan en un nivel de caliza margosa perteneciente a la Formación Galé (Miembro Água Doce, Albiense medio-base del superior). El estudio de láminas delgadas de los niveles C22/24 y C26 de la sección estratigráfica de Parede ha mostrado ricas biomicrofacies compuestas por foraminíferos, radiolarios, ostrácodos, corales, bivalvos, gasterópodos y equinoideos, mayoritariamente en textura “wackestone” con matriz biomicrítica. Estas asociaciones coinciden con las litofacies, los moluscos marinos, los equínidos y los icnofósiles obtenidos en la sección, y corresponden a un paleoambiente marino somero de plataforma interna con tendencia somerizante hacia techo. Las biofacies y el análisis secuencial resultan compatibles con la edad del inicio del Albiense tardío atribuida al yacimiento. Estas huellas y el moderado índice de dinoturbación son indicadores de la actividad de saurópodos en este paleoambiente. En base a la estrechez del rastro, probablemente a la morfología arriñonada/semicircular de la mano y al hecho que se trata de un rastro “pes-dominated”, pueden descartarse a los titanosaurios como posibles productores. Los rastros estrechos han sido relacionados con paleoambientes costeros, y el yacimiento de Parede sustenta esta interpretación. Además, este yacimiento proporciona nuevos datos sobre la presencia de rastros de tipo “pes-dominated” en substratos cohesivos. Los restos osteológicos de saurópodos cretácicos portugueses son muy escasos, aportando el yacimiento de Parede una nueva y relevante evidencia de estos dinosaurios. Asimismo, el yacimiento de Parede constituye el hallazgo más moderno de saurópodos en el registro portugués, y una de las pocas evidencias de saurópodos en Europa durante el Albiense. Este descubrimiento incrementa los datos paleobiogeográficos de Sauropoda en el Cretácico Inferior en la Península Ibérica, en un período de tiempo en el que los restos de estos dinosaurios son escasos en esta región del Suroeste de Europa. Por tanto, este hallazgo muestra un elevado interés debido a su trascendencia para la paleobiogeografía de Sauropoda en el Cretácico

Ichnological evidence of Megalosaurid Dinosaurs crossing Middle Jurassic tidal flats

A new dinosaur tracksite in the Vale de Meios quarry (Serra de Aire Formation, Bathonian, Portugal) preserves more than 700 theropod tracks. They are organized in at least 80 unidirectional trackways arranged in a bimodal orientation pattern (W/NW and E/SE). Quantitative and qualitative comparisons reveal that the large tridactyl, elongated and asymmetric tracks resemble the typical Late JurassicEarly Cretaceous Megalosauripus ichnogenus in all morphometric parameters. Few of the numerous tracks are preserved as elite tracks while the rest are preserved as different gradients of modified true tracks according to water content, erosive factors, radial fractures and internal overtrack formations. Taphonomical determinations are consistent with paleoenvironmental observations that indicate an inter-tidal flat located at the margin of a coastal barrier. The Megalosauripus tracks represent the oldest occurrence of this ichnotaxon and are attributed to large megalosaurid dinosaurs. Their occurrence in Vale de Meios tidal flat represents the unique paleoethological evidence of megalosaurids moving towards the lagoon, most likley during the low tide periods with feeding purposes

Sauropod trackways of the Iberian Peninsula: palaeoetological and palaeoenvironmental implications

More than a hundred sauropod tracksites, ranging in age from Middle Jurassic (Bathonian) to Late Cretaceous (Maastrichtian), are repor­ted from the Iberian Peninsula (Spain and Portugal). Sauropod trackway orientation patterns throughout an entire geographic area such as the Iberian Peninsula and over different time periods may provide new data about the palaeoecology and behavioural patterns of sauropods. The studied tracksites exhibit two main trackway orientation patterns, directional and random, indicating different behaviours in grega­rious or solitary (milling) individuals. Gregarious behavioural patterns are mainly observed within Parabrontopodus/Breviparopus-like and Brontopodus-like ichnotypes of a single size class (either small or medium-sized), indicating age segregation. Among the gregarious sauropods the former ichnotype is linked with inland environments, while the latter is mainly linked with coastal environments. Solitary trackways are mainly of medium-sized to large individuals, are also linked with both inland (Parabrontopodus/Breviparopus-like) and coastal environments (Parabrontopodus/Breviparopus-like, Brontopodus-like), and belong to the above-mentioned ichnotypes as well as to Polyonyx-like ichnotypes. Thus, no obvious link between gregarious behaviour and the palaeoenvironmental setting can be inferred from the sauropod track record of the Iberian Peninsula. Nonetheless, sauropod trackways showing titanosauriform characters (Brontopodus-like) are more common in coastal environments, contrary to what might be expected given this group’s apparent taxonomic preference for inland environments.El registro Ibérico (España y Portugal) de icnitas de saurópodos está representado por más de cien yacimientos cuya edad varía desde el Jurásico Medio (Batoniense) al Cretácico Superior (Maastrichtiense). El estudio de la orientación de los rastros, en un área determinada como la Península Ibérica y en diferentes edades, nos ha permitido obtener nuevos datos sobre el comportamiento de los saurópodos. Los rastros de los yacimientos estudiados presentan dos patrones de movimiento distintos, direccionales y aleatorios, que reflejan distintos com­portamientos en individuos gregarios o solitarios (deambulantes). El patrón que indica comportamiento gregario se observa en los icnotipos “Parabrontopodus/Breviparopus-like” y “Brontopodus-like” en saurópodos del mismo tamaño (pequeño o medio), lo que indica segregación por edad. El primer icnotipo se ha conservado en sedimentos depositados en ambientes terrestres mientras que el segundo lo hace fundamen­talmente en ambientes costeros. Los rastros solitarios son, en su mayoría, de saurópodos de tamaño medio o grande, conservados tanto en medios terrestres (“Parabrontopodus/Breviparopus-like”), como costeros (“Parabrontopodus/Breviparopus-like” y “Brontopodus-like”) y pertenecen a los dos icnotipos mencionados anteriormente y a un tercero: “Polyonyx-like”. Por lo tanto, no hay una significativa influencia paleoambiental en el registro del comportamiento gregario de los saurópodos ibéricos. Sin embargo, los rastros saurópodos que muestran caracteres Titanosauriformes (Brontopodus-like) son más frecuentes en los medios costeros de la Península Ibérica. Estos resultados son diferentes a los expuestos por otros autores que apuntan una aparente preferencia de los Titanosauriformes por los medios terrestres

LOWER CRETACEOUS DINOSAUR TRACKS FROM THE SEASHORE AREAS OF

Trata-se da descoberta de uma pista na praia de Parede, uma famosa área turística localizada a cerca de 20 km a partir de Lisboa, perto de Cascais, onde um dos poucos tracksites dinossauro conhecido do Cretáceo Inferior de Portugal podem ser observados. Uma visão geral da pista quase horizontal nível situado em frente ao "Bar Terraço Xana" põe em evidência uma sequência principal de pegadas interpretado como um saurópode trackway. Tem uma grande importância pedagógica - turística pela grande facilidade de acesso e observação

Intra-trackway morphological variations due to substrate consistency: the El Frontal dinosaur tracksite (Lower Cretaceous, Spain).

An ichnological and sedimentological study of the El Frontal dinosaur tracksite (Early Cretaceous, Cameros basin, Soria, Spain) highlights the pronounced intra-trackway variation found in track morphologies of four theropod trackways. Photogrammetric 3D digital models revealed various and distinct intra-trackway morphotypes, which reflect changes in footprint parameters such as the pace length, the track length, depth, and height of displacement rims. Sedimentological analyses suggest that the original substrate was non-homogenous due to lateral changes in adjoining microfacies. Multidata analyses indicate that morphological differences in these deep and shallow tracks represent a part of a continuum of track morphologies and geometries produced by a gradient of substrate consistencies across the site. This implies that the large range of track morphologies at this site resulted from similar trackmakers crossing variable facies. The trackways at the El Frontal site present an exemplary case of how track morphology, and consequently potential ichnotaxa, can vary, even when produced by a single trackmaker

101 Dothideomycetes genomes: A test case for predicting lifestyles and emergence of pathogens.

Dothideomycetes is the largest class of kingdom Fungi and comprises an incredible diversity of lifestyles, many of which have evolved multiple times. Plant pathogens represent a major ecological niche of the class Dothideomycetes and they are known to infect most major food crops and feedstocks for biomass and biofuel production. Studying the ecology and evolution of Dothideomycetes has significant implications for our fundamental understanding of fungal evolution, their adaptation to stress and host specificity, and practical implications with regard to the effects of climate change and on the food, feed, and livestock elements of the agro-economy. In this study, we present the first large-scale, whole-genome comparison of 101 Dothideomycetes introducing 55 newly sequenced species. The availability of whole-genome data produced a high-confidence phylogeny leading to reclassification of 25 organisms, provided a clearer picture of the relationships among the various families, and indicated that pathogenicity evolved multiple times within this class. We also identified gene family expansions and contractions across the Dothideomycetes phylogeny linked to ecological niches providing insights into genome evolution and adaptation across this group. Using machine-learning methods we classified fungi into lifestyle classes with >95 % accuracy and identified a small number of gene families that positively correlated with these distinctions. This can become a valuable tool for genome-based prediction of species lifestyle, especially for rarely seen and poorly studied species

An overview of the lower cretaceous dinosaur tracksites from the mirambel formation in the iberian range (ne spain)

Up to now, the ichnological vertebrate record from the Barremian Mirambel Formation (NE Spain) has remained completely unknown despite the fact that osteological findings have been reported in recent years. Here we provide an overview of 11 new dinosaur tracksites found during a fieldwork campaign in the year 2011. The majority of these tracksites (seven) preserve small- to medium-sized tridactyl tracks here assigned to indeterminate theropods. Only one footprint presents enough characters to classify it as Megalosauripus isp. Ornithopod tracks identified as Caririchnium isp. and Iguanodontipodidae indet. and sauropod tracks are recorded at two tracksites. The footprints are preserved in a variety of paleoenvironmental conditions and thus display different kinds of preservation (true tracks, shallow undertracks, natural casts and undertrack casts). The ichnological record from the Mirambel Formation seems to be theropod dominated. This is a clear discrepancy with the osteological record identified in this formation, which shows a predominance of ornithopod dinosaurs

Manus track preservation bias as a key factor for assessing trackmaker identity and quadrupedalism in basal ornithopods.

BACKGROUND: The Las Cerradicas site (Tithonian-Berriasian), Teruel, Spain, preserves at least seventeen dinosaur trackways, some of them formerly attributed to quadrupedal ornithopods, sauropods and theropods. The exposure of new track evidence allows a more detailed interpretation of the controversial tridactyl trackways as well as the modes of locomotion and taxonomic affinities of the trackmakers. METHODOLOGY/PRINCIPAL FINDINGS: Detailed stratigraphic analysis reveals four different levels where footprints have been preserved in different modes. Within the tridactyl trackways, manus tracks are mainly present in a specific horizon relative to surface tracks. The presence of manus tracks is interpreted as evidence of an ornithopod trackmaker. Cross-sections produced from photogrammetric digital models show different depths of the pes and manus, suggesting covariance in loading between the forelimbs and the hindlimbs. CONCLUSIONS/SIGNIFICANCE: Several features (digital pads, length/width ratio, claw marks) of some ornithopod pes tracks from Las Cerradicas are reminiscent of theropod pedal morphology. This morphological convergence, combined with the shallow nature of the manus tracks, which reduces preservation potential, opens a new window into the interpretation of these tridactyl tracks. Thus, trackmaker assignations during the Jurassic-Cretaceous interval of purported theropod trackways may potentially represent ornithopods. Moreover, the Las Cerradicas trackways are further evidence for quadrupedalism among some basal small- to medium-sized ornithopods from this time interval

Enigmatic tracks of solitary sauropods roaming an extensive lacustrine megatracksite in Iberia

Sauropod remains are abundant on the Iberian Peninsula across the Jurassic-Cretaceous transition. Where the osteological record shows a high diversity of this kind of dinosaur, the ichnological findings are mainly limited to sauropod tracks characterized by kidney-shaped manus (with or without pollex impressions) and pes impressions with three claw imprints oriented laterally. Here, we present a new sauropod ichnotaxon, Iniestapodus burgensis, found at several exposures within the Las Sereas megatracksite (Burgos, Spain). These are preserved within lacustrine limestone strata of the Rupelo Formation (Tithonian-Berriasian). Iniestapodus burgensis is characterized by: semicircular manus tracks with small pollex impressions; unusual tetradactyl pes tracks with evidence of four claws oriented anteriorly (I-II) and laterally (III-IV), of variable sizes (short claw I and IV impressions, claw II and III being the largest). The combination of features and comparison with the osteological record allows us to propose a non-titanosaurian titanosauriform as a possible trackmaker. All the Iniestapodus tracks are represented by at least two different size classes of small and medium-sized individuals, and their trackways show different multidirectional orientations. The paleoenvironmental and paleoecological data suggest that Iniestapodus trackmakers were solitary individuals, likely representing different age classes, that crossed and used the Las Sereas shallow lacustrine-palustrine areas as their preferred habitat

101 Dothideomycetes genomes: a test case for predicting lifestyles and emergence of pathogens

Dothideomycetes is the largest class of kingdom Fungi and comprises an incredible diversity of lifestyles, many of which have evolved multiple times. Plant pathogens represent a major ecological niche of the class Dothideomycetes and they are known to infect most major food crops and feedstocks for biomass and biofuel production. Studying the ecology and evolution of Dothideomycetes has significant implications for our fundamental understanding of fungal evolution, their adaptation to stress and host specificity, and practical implications with regard to the effects of climate change and on the food, feed, and livestock elements of the agro-economy. In this study, we present the first large-scale, whole-genome comparison of 101 Dothideomycetes introducing 55 newly sequenced species. The availability of whole-genome data produced a high-confidence phylogeny leading to reclassification of 25 organisms, provided a clearer picture of the relationships among the various families, and indicated that pathogenicity evolved multiple times within this class. We also identified gene family expansions and contractions across the Dothideomycetes phylogeny linked to ecological niches providing insights into genome evolution and adaptation across this group. Using machine-learning methods we classified fungi into lifestyle classes with >95 % accuracy and identified a small number of gene families that positively correlated with these distinctions. This can become a valuable tool for genome-based prediction of species lifestyle, especially for rarely seen and poorly studied species.This manuscript has been authored by an author at Lawrence Berkeley National Laboratory under Contract No. DE-AC02-05CH11231 with the U.S. Department of Energy. R. A. and I. V. G. were supported by the NSF grants DEB-1354625 and IOS-1456958. Sequencing of Dothideomycetes genomes were conducted as part of the 1000 Fungal Genomes Project supported by the JGI Community Sequencing Project #662