Primary school education in the time of covid-19:a literature review

Abstract. The impact of the COVID-19 global pandemic which originated in Wuhan in 2019 has had an unprecedented impact on everyday life over the past year. This is especially true for both students and teachers across the globe, with UNESCO (2020) estimating that school closures had affected over one and a half billion students across the globe at the end of 2020. Due to the pandemic teachers were suddenly forced with little warning, or training to adapt their teaching and pedagogical approaches from face-to-face teaching to distance and hybrid teaching. This thesis examines, via means of a literature review, how well primary school teachers were prepared for this shift, the role of technology in their teaching and the evolution of teaching practises during this shift to distant education. Additionally, support mechanisms available for teachers during this shift to distance teaching were also examined. The theoretical background of the thesis first explores two educational trends, providing a definition, brief history, and different models available. The first educational theory is Computer-Based Education, a framework in which technology serves as a learning tool, facilitating learning through different practices, apps, and multimedia content; the second is distance education, which can simply be defined as “any form of providing education to students who are separated by distance (i.e., who are not physically present in the same space) and in which the pedagogical material is planned and prepared by an educational institution ranging from the first examples of correspondence courses arriving to today’s technology-based synchronous and asynchronous courses. The first research question shows how, despite a very limited number of exceptions, the literature available and the surveys conducted in different areas of the world report a certain degree of insecurity among teachers in switching from face-to-face to distance education for different reasons, such as lack of training, confidence or appliances. The thesis follows with the exploration of the role of technology in distance education and how the teaching practices evolved during social distancing, highlighting how tools such as videoconferencing became widespread in the teaching practices. Concerning changes in teacher education according to the standards of distance education, some pre pandemic frameworks are provided in order to prepare teachers better for ‘emergency remote teaching’ despite the literature on the topic is still limited. In addition to examining the issues facing teachers, several support mechanisms and educational technology solutions were also identified at the regional, national and community level. The private and public sectors have also provided a multitude of Educational technology solutions which teachers have also had the opportunity to utilise in the shift to online teaching

The Role of Eif6 in Skeletal Muscle Homeostasis Revealed by Endurance Training Co-expression Networks

Regular endurance training improves muscle oxidative capacity and reduces the risk of age-related disorders. Understanding the molecular networks underlying this phenomenon is crucial. Here, by exploiting the power of computational modeling, we show that endurance training induces profound changes in gene regulatory networks linking signaling and selective control of translation to energy metabolism and tissue remodeling. We discovered that knockdown of the mTOR-independent factor Eif6, which we predicted to be a key regulator of this process, affects mitochondrial respiration efficiency, ROS production, and exercise performance. Our work demonstrates the validity of a data-driven approach to understanding muscle homeostasis

Molecular systematics of the genus Artibeus (Chiroptera

a b s t r a c t A molecular phylogeny of the genus Artibeus using 19 of the 20 recognized species, many with samples from a broad geographic range, is presented. The analysis shows a clear distinction between the two subgenera (or genera), the 'large' Artibeus and the 'small' Dermanura, in both mitochondrial and nuclear genes. The placement and status of A. concolor remains inconclusive and is presented as the third subgenus Koopmania. The phylogenies and divergence time estimates show a marked influence of the Andes in the formation of the subgenera and the main lineages inside each subgenus. Nuclear genes showed a highly incomplete lineage sorting among species inside subgenera Artibeus and Dermanura. Indeed, shared alleles were also found between Artibeus and Koopmania, which are presumed to have split apart during the Miocene, showing that great care should be taken in using these markers. Cytochrome-b gene divergences and monophyly analyses suggest that A. lituratus and A. intermedius are indeed conspecifics. These analyses also suggested the existence of at least four 'new' species revealing a significant cryptic diversity inside the genus

Compartmentalized activities of the pyruvate dehydrogenase complex sustain lipogenesis in prostate cancer.

The mechanisms by which mitochondrial metabolism supports cancer anabolism remain unclear. Here, we found that genetic and pharmacological inactivation of pyruvate dehydrogenase A1 (PDHA1), a subunit of the pyruvate dehydrogenase complex (PDC), inhibits prostate cancer development in mouse and human xenograft tumor models by affecting lipid biosynthesis. Mechanistically, we show that in prostate cancer, PDC localizes in both the mitochondria and the nucleus. Whereas nuclear PDC controls the expression of sterol regulatory element-binding transcription factor (SREBF)-target genes by mediating histone acetylation, mitochondrial PDC provides cytosolic citrate for lipid synthesis in a coordinated manner, thereby sustaining anabolism. Additionally, we found that PDHA1 and the PDC activator pyruvate dehydrogenase phosphatase 1 (PDP1) are frequently amplified and overexpressed at both the gene and protein levels in prostate tumors. Together, these findings demonstrate that both mitochondrial and nuclear PDC sustain prostate tumorigenesis by controlling lipid biosynthesis, thus suggesting this complex as a potential target for cancer therapy

HER3 Is an Actionable Target in Advanced Prostate Cancer

\ua92021 The Authors; Published by the American Association for Cancer Research. It has been recognized for decades that ERBB signaling is important in prostate cancer, but targeting ERBB receptors as a therapeutic strategy for prostate cancer has been ineffective clinically. However, we show here that membranous HER3 protein is commonly highly expressed in lethal prostate cancer, associating with reduced time to castration resistance (CR) and survival. Multiplex immunofluorescence indicated that the HER3 ligand NRG1 is detectable primarily in tumor-infiltrating myelomonocytic cells in human prostate cancer; this observation was confirmed using single-cell RNA sequencing of human prostate cancer biopsies and murine transgenic prostate cancer models. In castration-resistant prostate cancer (CRPC) patient-derived xenograft organoids with high HER3 expression as well as mouse prostate cancer organoids, recombinant NRG1 enhanced proliferation and survival. Supernatant from murine bone marrow–derived macrophages and myeloid-derived suppressor cells promoted murine prostate cancer organoid growth in vitro, which could be reversed by a neutralizing anti-NRG1 antibody and ERBB inhibition. Targeting HER3, especially with the HER3-directed antibody–drug conjugate U3-1402, exhibited antitumor activity against HER3-expressing prostate cancer. Overall, these data indicate that HER3 is commonly overexpressed in lethal prostate cancer and can be activated by NRG1 secreted by myelomonocytic cells in the tumor microenvironment, supporting HER3-targeted therapeutic strategies for treating HER3-expressing advanced CRPC

HER3 Is an Actionable Target in Advanced Prostate Cancer.

It has been recognized for decades that ERBB signaling is important in prostate cancer, but targeting ERBB receptors as a therapeutic strategy for prostate cancer has been ineffective clinically. However, we show here that membranous HER3 protein is commonly highly expressed in lethal prostate cancer, associating with reduced time to castration resistance (CR) and survival. Multiplex immunofluorescence indicated that the HER3 ligand NRG1 is detectable primarily in tumor-infiltrating myelomonocytic cells in human prostate cancer; this observation was confirmed using single-cell RNA sequencing of human prostate cancer biopsies and murine transgenic prostate cancer models. In castration-resistant prostate cancer (CRPC) patient-derived xenograft organoids with high HER3 expression as well as mouse prostate cancer organoids, recombinant NRG1 enhanced proliferation and survival. Supernatant from murine bone marrow-derived macrophages and myeloid-derived suppressor cells promoted murine prostate cancer organoid growth in vitro, which could be reversed by a neutralizing anti-NRG1 antibody and ERBB inhibition. Targeting HER3, especially with the HER3-directed antibody-drug conjugate U3-1402, exhibited antitumor activity against HER3-expressing prostate cancer. Overall, these data indicate that HER3 is commonly overexpressed in lethal prostate cancer and can be activated by NRG1 secreted by myelomonocytic cells in the tumor microenvironment, supporting HER3-targeted therapeutic strategies for treating HER3-expressing advanced CRPC. SIGNIFICANCE: HER3 is an actionable target in prostate cancer, especially with anti-HER3 immunoconjugates, and targeting HER3 warrants clinical evaluation in prospective trials

Cervidae antlers exploited to manufacture prehistoric tools and hunting implements as a reliable source of ancient DNA

Antler is one of the primary animal raw materials exploited for technical purposes by the huntergatherer groups of the Eurasian Upper Palaeolithic (UP) all over the ecological range of deers, and beyond. It was exhaustively employed to produce one of the most critical tools for the survival of the UP societies: hunting weapons. However, antler implements can be made from diverse deer taxa, with different ecological requirements and ethological behaviours. Identifying the antler's origin at a taxonomic level is thus essential in improving our knowledge of humans' functional, practical and symbolic choices, as well as the human-animal interface during Prehistoric times. Nevertheless, palaeogenetics analyses have focused mainly on bone and teeth, with genetic studies of antler generally focused on modern deer conservation. Here we present the results of the first whole mitochondrial genome ancient DNA (aDNA) analysis by means of in-solution hybridisation capture of antlers from pre-Holocene archaeological contexts. We analysed a set of 50 Palaeolithic and Neolithic (c. 34-8ka) antler and osseous objects from South-Western Europe, Central Europe, South-Western Asia and the Caucasus. We successfully obtained aDNA, allowing us to identify the exploited taxa and demonstrate the archaeological relevance of those finds. Moreover, as most of the antlers were sampled using a minimally-invasive method, further analyses (morphometric, technical, genetic, radiometric and more) remain possible on these objects.Research of J.-M. T. is supported by a project of the Meitner Program of the Austrian Science Fund (FWF) (Project: Osseous Hunting Weapons of Early Modern Humans in Eurasia. Number M3112) and the program Ram´on y Cajal of the Spanish MCIN/AEI (MCIN/AEI/10.13039/501100011033. Project Number RYC2021-033759-I) and the European Community (NextGenerationEU»/PRTR). The University of Vienna Research Platform: Mineralogical Preservation of the Human Biome from the Depth of Time (MINERVA) supported the whole project. J.-M. T., P. G., and O. C. benefited from a Seed Grant from the HEAS (Human Evolution and Archaeological Sciences) of the University of Vienna (Project: Assessing the differential DNA preservation in Palaeolithic sediments and osseous tools from museum collections). D. M. B. supported by a Seal of Excellence Fellowship of the Austrian Academy of Sciences (‘TechnoBeads’ project no. 101061287). P. R. N. benefited from funding from the University of Vienna and the Land Nieder¨osterreich, Abteilung Wissenschaft & Forschung (project K3–F-530/005–2021). Research at La Garma (P.A. and R.O) is included in the R&D project PID2020-112832RBI00, funded by the Spanish Ministry of Science and Innovation (MCIN/AEI/10.13039/501100011033). Research at Tito Bustillo Cave (E.A.F, M.C. and J.T.) is included in the Project PID2020-114462GB-I00/AEI/10.13039/ 501100011033, funded by the Spanish Ministry of Science and Innovation

Coagulation factor X promotes resistance to androgen-deprivation therapy in prostate cancer

\ua9 2024 The Author(s). Although hypercoagulability is commonly associated with malignancies, whether coagulation factors directly affect tumor cell proliferation remains unclear. Herein, by performing single-cell RNA sequencing (scRNA-seq) of the prostate tumor microenvironment (TME) of mouse models of castration-resistant prostate cancer (CRPC), we report that immunosuppressive neutrophils (PMN-MDSCs) are a key extra-hepatic source of coagulation factor X (FX). FX activation within the TME enhances androgen-independent tumor growth by activating the protease-activated receptor 2 (PAR2) and the phosphorylation of ERK1/2 in tumor cells. Genetic and pharmacological inhibition of factor Xa (FXa) antagonizes the oncogenic activity of PMN-MDSCs, reduces tumor progression, and synergizes with enzalutamide therapy. Intriguingly, F10high PMN-MDSCs express the surface marker CD84 and CD84 ligation enhances F10 expression. Elevated levels of FX, CD84, and PAR2 in prostate tumors associate with worse survival in CRPC patients. This study provides evidence that FXa directly promotes cancer and highlights additional targets for PMN-MDSCs for cancer therapies