Advanced optical microscopies for materials: new trends

Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166This article summarizes the new trends of Optical Microscopy applied to Materials, with examples of applications that illustrate the capabilities of the technique

Las teorías implícitas de los profesores de Educación Física

Este trabajo forma parte de una investigación mucho más amplia que, tomando la programación de la Educación Física como eje referencial, pretende estudiar el pensamiento del profesor y analizar los factores que inciden en las decisiones que éste toma cuando resuelve ciertos aspectos vinculados con sus tareas docentes. Entre el conjunto de aspectos que condicionan las diferentes decisiones del profesor se encuentran las teorías implícitas que éste posee respecto a la enseñanza. Estas teorías son el conjunto de creencias personales que rigen la manera de comportarse de los docentes delante de situaciones concretas. El presente estudio trata de acercarse al conocimiento de estas teorías y analiza con detalle la presencia y la incidencia de las mismas en el pensamiento de los docentes y sus consecuencias para el proceso de enseñanza y aprendizaje. La investigación sigue un diseño transversal y trata de describir a la vez de interpretar el cúmulo de factores que forman estas teorías en los docentes de las etapas obligatorias de educación

NO3- selective mini-electrodes as a tool to investigate the NO3- traffic in Chlamydomonas reinhardtii D.

Ion selective NO3- mini-electrodes were used to measure the external NO3- concentration in C. reinhardtii liquid cultures. Electrodes were prepared using glass capillaries (1.5 mm external diameter). Capillaries were cut in 10 cm long pieces, dehydrated for 45 minutes in an oven and silanized by addition of dimethyldichlorosilane in bencene 0.1% (V/V). Once silanized, the capillaries were baked again for 30 minutes. Once cold the capillaries were backfilled with the NO3- ionophore (Fluka: 72549), which contains PVC (5.75% w/w) dissolved in tetrahydrofurane. Then, the NO3- mini-electrodes were stored in dark in a desiccator until tetrahydrofurane gets evaporated. Before use, NO3- selective mini-electrodes were backfilled with 0.1 M NaNO3 and 0.1 M KCl and connected to a high-impedance differential amplifier (WPI FD223). Mini-electrodes were calibrated in N-free Beijerinck medium, which contains 0.1 mM Cl-. In those conditions, electrodes calibration slope was 54 mV/p NO3- in the range 1 - 1000 µM NO3-. The mini-electrodes were used to continuous monitoring of the external NO3- concentration in liquid culture of different C. reinhardtii strains, incubated in N-free Beijerinck medium supplemented with 100 µM NO3Na. Previous to the assays, strains were N starved for 6 days. In the light, wild type strain uptakes NO3- at a rate of 15 nmol NO3-·106 cells-1·h-1, in the dark this rate was one third of this figure. After 5 h, the external NO3- levelled off at 10 µM in the light and around 30 µM in the dark. C. reinhardtii cells cultured in the presence of 2 mM NO3NH4 do not show significant NO3- uptake nor a mutant strain, defective in nitrate transport and having an active nitrate reductase. However, a mutant strain lacking the nitrate reductase shows an enhanced NO3- uptake rate, compared with the value obtained for the wild type in the light.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

HCO3- enrichment causes cytosolic NO3- efflux in Posidonia oceanica leaf cells

Posidonia oceanica is a seagrass, the only group of vascular plants to colonize the marine environment. Seawater is an extreme yet stable environment, characterized by high salinity, alkaline pH and low availability of essential nutrients, such as nitrate or phosphate. In addition, in aquatic environments the supply of CO2 for the photosynthesis is limited by diffusion and therefore many aquatic plants use HCO3- as the inorganic carbon source for photosynthesis. Previous results have shown that Na+ -dependent transport systems operate on the plasma membrane of P. oceanica mesophyll leaf cells for the high-affinity NO3-, Pi or amino acids uptake. Also, a direct transport of HCO3- driven by H+ has been found in this species that provides inorganic carbon for photosynthesis and could be a significant component of a carbon concentrating mechanism in this species. Interestingly, this HCO3- direct uptake caused the efflux of chloride from the cytosol, probably through S-type anion channels, pointing that other anions could also be removed from the cytosol. This hypothesis could be relevant in the case of NO3-, since the decrease of cytosolic NO3- in response to HCO3- enrichment could limit N-assimilation. Here we analyse the effect of HCO3- increase on NO3- uptake and cytosolic homeostasis in P. oceanica. Enrichment of natural seawater with 3 mM HCO3- evokes the on-going decrease of cytosolic NO3-, from 5.7 ± 0.2 to 4.8 ± 0.7 mM after 40 min of treatment. The incubation of P. oceanica leaf pieces in 3 mM HCO3- NSW causes an initial increase of NO3- concentration in the medium. Maximum efflux (21 nmol NO3- gFM-1 min-1) occurs within the first minute of incubation. Then, external NO3- is depleted from the medium at lower net uptake rate than the value observed in non HCO3- -enriched natural seawater. These results fit the hypothesis that HCO3- enrichment causes the nitrogen loose and could impair nitrogen assimilation promoting N biomass impoverishment.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Spanish MINECO, projects BFU2017-85117-R and BIO2016-81957-RED

Linear orderings of random geometric graphs (extended abstract)

In random geometric graphs, vertices are randomly distributed on [0,1]^2 and pairs of vertices are connected by edges whenever they are sufficiently close together. Layout problems seek a linear ordering of the vertices of a graph such that a certain measure is minimized. In this paper, we study several layout problems on random geometric graphs: Bandwidth, Minimum Linear Arrangement, Minimum Cut, Minimum Sum Cut, Vertex Separation and Bisection. We first prove that some of these problems remain \NP-complete even for geometric graphs. Afterwards, we compute lower bounds that hold with high probability on random geometric graphs. Finally, we characterize the probabilistic behavior of the lexicographic ordering for our layout problems on the class of random geometric graphs.Postprint (published version

Convergence theorems for some layout measures on random lattice and random geometric graphs

This work deals with convergence theorems and bounds on the cost of several layout measures for lattice graphs, random lattice graphs and sparse random geometric graphs. For full square lattices, we give optimal layouts for the problems still open. Our convergence theorems can be viewed as an analogue of the Beardwood, Halton and Hammersley theorem for the Euclidian TSP on random points in the $d$-dimensional cube. As the considered layout measures are non-subadditive, we use percolation theory to obtain our results on random lattices and random geometric graphs. In particular, we deal with the subcritical regimes on these class of graphs.Postprint (published version